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P35129

- UBC2_CAEEL

UniProt

P35129 - UBC2_CAEEL

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Protein

Ubiquitin-conjugating enzyme E2 2

Gene
let-70, ubc-2, M7.1
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin protein ligase binding Source: WormBase
  4. ubiquitin-protein transferase activity Source: WormBase

GO - Biological processi

  1. protein ubiquitination Source: WormBase
  2. protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_107985. Signaling by BMP.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 2 (EC:6.3.2.19)
Alternative name(s):
Lethal protein 70
Ubiquitin carrier protein 2
Ubiquitin-protein ligase 2
Gene namesi
Name:let-70
Synonyms:ubc-2
ORF Names:M7.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome IV

Organism-specific databases

WormBaseiM7.1; CE03482; WBGene00002344; let-70.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 147147Ubiquitin-conjugating enzyme E2 2PRO_0000082515Add
BLAST

Proteomic databases

PaxDbiP35129.
PRIDEiP35129.

Interactioni

Protein-protein interaction databases

BioGridi43106. 5 interactions.
DIPiDIP-24306N.
IntActiP35129. 2 interactions.
MINTiMINT-1108744.
STRINGi6239.M7.1.

Structurei

Secondary structure

147
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1 – 1515
Beta strandi19 – 268
Beta strandi29 – 3810
Turni44 – 474
Beta strandi49 – 557
Turni58 – 614
Beta strandi66 – 716
Helixi87 – 893
Turni90 – 923
Helixi99 – 11113
Helixi121 – 1299
Helixi131 – 14515

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z2UX-ray1.10A1-147[»]
ProteinModelPortaliP35129.
SMRiP35129. Positions 1-147.

Miscellaneous databases

EvolutionaryTraceiP35129.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00740000115162.
HOGENOMiHOG000233455.
InParanoidiP35129.
KOiK06689.
OMAiNDLGRDP.
PhylomeDBiP35129.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35129-1 [UniParc]FASTAAdd to Basket

« Hide

MALKRIQKEL QDLGRDPPAQ CSAGPVGDDL FHWQATIMGP PESPYQGGVF    50
FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS 100
KVLLSICSLL CDPNPDDPLV PEIARIYKTD RERYNQLARE WTQKYAM 147
Length:147
Mass (Da):16,705
Last modified:February 1, 1994 - v1
Checksum:i9E91884D307F2120
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti75 – 751H → Y in let-70(S1132); which shows growth arrest in larval stage.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S56051 Genomic DNA. Translation: AAB25489.2.
Z68337 Genomic DNA. Translation: CAA92745.1.
PIRiA48145.
T23820.
RefSeqiNP_502065.1. NM_069664.4.
UniGeneiCel.18192.

Genome annotation databases

EnsemblMetazoaiM7.1; M7.1; WBGene00002344.
GeneIDi178006.
KEGGicel:CELE_M7.1.
UCSCiM7.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S56051 Genomic DNA. Translation: AAB25489.2 .
Z68337 Genomic DNA. Translation: CAA92745.1 .
PIRi A48145.
T23820.
RefSeqi NP_502065.1. NM_069664.4.
UniGenei Cel.18192.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Z2U X-ray 1.10 A 1-147 [» ]
ProteinModelPortali P35129.
SMRi P35129. Positions 1-147.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 43106. 5 interactions.
DIPi DIP-24306N.
IntActi P35129. 2 interactions.
MINTi MINT-1108744.
STRINGi 6239.M7.1.

Proteomic databases

PaxDbi P35129.
PRIDEi P35129.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai M7.1 ; M7.1 ; WBGene00002344 .
GeneIDi 178006.
KEGGi cel:CELE_M7.1.
UCSCi M7.1. c. elegans.

Organism-specific databases

CTDi 178006.
WormBasei M7.1 ; CE03482 ; WBGene00002344 ; let-70.

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00740000115162.
HOGENOMi HOG000233455.
InParanoidi P35129.
KOi K06689.
OMAi NDLGRDP.
PhylomeDBi P35129.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_107985. Signaling by BMP.

Miscellaneous databases

EvolutionaryTracei P35129.
NextBioi 899334.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The ubc-2 gene of Caenorhabditis elegans encodes a ubiquitin-conjugating enzyme involved in selective protein degradation."
    Zhen M., Heinlein R., Jones D., Jentsch S., Candido E.P.M.
    Mol. Cell. Biol. 13:1371-1377(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "An essential ubiquitin-conjugating enzyme with tissue and developmental specificity in the nematode Caenorhabditis elegans."
    Zhen M., Scheni J.E., Baillie D.L., Candido E.P.M.
    EMBO J. 15:3229-3237(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION AS LET-70.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiUBC2_CAEEL
AccessioniPrimary (citable) accession number: P35129
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 3, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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