Ubiquitin-conjugating enzyme E2 2 - Caenorhabditis elegans

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Reviewed, UniProtKB/Swiss-Prot P35129 (UBC2_CAEEL)

Last modified June 16, 2009. Version 74. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 2
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase 2
    Ubiquitin carrier protein 2
    Lethal protein 70

Gene names

Name:	let-70
Synonyms:	ubc-2
ORF Names:	M7.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

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Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	determination of adult life span Inferred from mutant phenotype. Source: WormBase embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW nematode larval development Inferred from mutant phenotype. Source: WormBase post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
C25D7.8	Q9XVR6	1	EBI-316050,EBI-316044

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 147

147

Ubiquitin-conjugating enzyme E2 2

PRO_0000082515

Sites

Active site

Glycyl thioester intermediate By similarity

Natural variations

Natural variant

H → Y in let-70(S1132); which shows growth arrest in larval stage.

Secondary structure

147

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P35129-1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 9E91884D307F2120
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FASTA

147

16,705

        10         20         30         40         50         60 
MALKRIQKEL QDLGRDPPAQ CSAGPVGDDL FHWQATIMGP PESPYQGGVF FLTIHFPTDY 

        70         80         90        100        110        120 
PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV 

       130        140 
PEIARIYKTD RERYNQLARE WTQKYAM

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ubc-2 gene of Caenorhabditis elegans encodes a ubiquitin-conjugating enzyme involved in selective protein degradation." Zhen M., Heinlein R., Jones D., Jentsch S., Candido E.P.M. Mol. Cell. Biol. 13:1371-1377(1993) [PubMed: 8441382] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"An essential ubiquitin-conjugating enzyme with tissue and developmental specificity in the nematode Caenorhabditis elegans." Zhen M., Scheni J.E., Baillie D.L., Candido E.P.M. EMBO J. 15:3229-3237(1996) [PubMed: 8670823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION AS LET-70.
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

S56051 Genomic DNA. Translation: AAB25489.2.
Z68337 Genomic DNA. Translation: CAA92745.1.

PIR

A48145.
T23820.

RefSeq

NP_502065.1.

UniGene

Cel.18192

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Z2U	X-ray	1.10	A	1-147	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:24306N.

IntAct

P35129. 3 interactions.

Genome annotation databases

Ensembl

M7.1. Caenorhabditis elegans. [Contig view]

GeneID

178006.

KEGG

cel:M7.1.

NMPDR

fig|6239.3.peg.15092.

Organism-specific databases

WormBase

WBGene00002344. let-70.

WormPep

M7.1. CE03482. [WorfDB]

Phylogenomic databases

OMA

P35129. NFTTRIY.

Enzyme and pathway databases

BRENDA

6.3.2.19. 672.

Gene expression databases

ArrayExpress

P35129.

Family and domain databases

InterPro

IPR016135. UBQ-conjugat/RWD-like.
IPR000608. UBQ-conjugat_E2.
[Graphical view]

Gene3D

G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

ProDom

PD000461. UBQ_conjugat. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00212. UBCc. 1 hit.
[Graphical view]

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

899334.

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Entry information

Entry name

UBC2_CAEEL

Accession

Primary (citable) accession number: P35129

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sites

Natural variations

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents