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Protein

Ubiquitin-conjugating enzyme E2 2

Gene

let-70

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity). Mediates the selective degradation of short-lived and abnormal proteins. Plays a role in the DNA damage response (PubMed:16628214). In particular, in response to ionizing radiation, associates with the E3 ubiquitin-protein ligase brc-1-brd-1 heterodimer on chromatin to activate E3-ubiquitin ligase activity of the heterodimer, and thus its DNA damage repair mechanisms (PubMed:16628214).By similarityCurated1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin protein ligase binding Source: WormBase
  • ubiquitin-protein transferase activity Source: WormBase

GO - Biological processi

  • protein ubiquitination Source: WormBase
  • ubiquitin-dependent protein catabolic process Source: WormBase

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-CEL-201451 Signaling by BMP
R-CEL-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 2 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 2
Lethal protein 70
Ubiquitin carrier protein 2
Ubiquitin-protein ligase 2
Gene namesi
Name:let-701 PublicationImported
Synonyms:ubc-21 PublicationImported
ORF Names:M7.1Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiM7.1 ; CE03482 ; WBGene00002344 ; let-70

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown results in reduced ubiquitination at DNA damage sites.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi75H → Y in s1132; hermaphrodites arrest during the early stages of larval stages. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825151 – 147Ubiquitin-conjugating enzyme E2 2Add BLAST147

Proteomic databases

EPDiP35129
PaxDbiP35129
PeptideAtlasiP35129
PRIDEiP35129

Expressioni

Tissue specificityi

Expressed in the nervous system.1 Publication

Developmental stagei

Expressed at all stages of development (PubMed:8441382, PubMed:8670823). Expressed in most embryonic tissues and larval tissues at the early stages (PubMed:8670823). Highly expressed in neurons, pharynx, the hyperdermis and body muscles in L1, L2, L3 and dauer larvae (PubMed:8670823). From the L4 stage of larval development and in adults, expression is mainly restricted to the nervous system, but is also expressed in the pharynx and hyperdermis (PubMed:8670823).2 Publications

Gene expression databases

BgeeiWBGene00002344

Interactioni

Subunit structurei

Interacts with the brc-1-brd-1 heterodimer following ionizing irradiation.1 Publication

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: WormBase

Protein-protein interaction databases

BioGridi43106, 5 interactors
DIPiDIP-24306N
IntActiP35129, 3 interactors
STRINGi6239.M7.1.1

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 15Combined sources15
Beta strandi19 – 26Combined sources8
Beta strandi29 – 38Combined sources10
Turni44 – 47Combined sources4
Beta strandi49 – 55Combined sources7
Turni58 – 61Combined sources4
Beta strandi66 – 71Combined sources6
Helixi87 – 89Combined sources3
Turni90 – 92Combined sources3
Helixi99 – 111Combined sources13
Helixi121 – 129Combined sources9
Helixi131 – 145Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z2UX-ray1.10A1-147[»]
ProteinModelPortaliP35129
SMRiP35129
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35129

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00910000143990
HOGENOMiHOG000233455
InParanoidiP35129
KOiK06689
OMAiNDLGRDP
OrthoDBiEOG091G0GF8
PhylomeDBiP35129

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P35129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKRIQKEL QDLGRDPPAQ CSAGPVGDDL FHWQATIMGP PESPYQGGVF
60 70 80 90 100
FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS
110 120 130 140
KVLLSICSLL CDPNPDDPLV PEIARIYKTD RERYNQLARE WTQKYAM
Length:147
Mass (Da):16,705
Last modified:February 1, 1994 - v1
Checksum:i9E91884D307F2120
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S56051 Genomic DNA Translation: AAB25489.2
Z68337 Genomic DNA Translation: CAA92745.1
PIRiA48145
T23820
RefSeqiNP_502065.1, NM_069664.4
UniGeneiCel.18192

Genome annotation databases

EnsemblMetazoaiM7.1; M7.1; WBGene00002344
GeneIDi178006
KEGGicel:CELE_M7.1
UCSCiM7.1 c. elegans

Similar proteinsi

Entry informationi

Entry nameiUBC2_CAEEL
AccessioniPrimary (citable) accession number: P35129
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 25, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health