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P35129 (UBC2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 2

EC=6.3.2.19
Alternative name(s):
Lethal protein 70
Ubiquitin carrier protein 2
Ubiquitin-protein ligase 2
Gene names
Name:let-70
Synonyms:ubc-2
ORF Names:M7.1
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length147 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 147147Ubiquitin-conjugating enzyme E2 2
PRO_0000082515

Sites

Active site851Glycyl thioester intermediate By similarity

Natural variations

Natural variant751H → Y in let-70(S1132); which shows growth arrest in larval stage.

Secondary structure

....................... 147
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P35129 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 9E91884D307F2120

FASTA14716,705
        10         20         30         40         50         60 
MALKRIQKEL QDLGRDPPAQ CSAGPVGDDL FHWQATIMGP PESPYQGGVF FLTIHFPTDY 

        70         80         90        100        110        120 
PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV 

       130        140 
PEIARIYKTD RERYNQLARE WTQKYAM 

« Hide

References

« Hide 'large scale' references
[1]"The ubc-2 gene of Caenorhabditis elegans encodes a ubiquitin-conjugating enzyme involved in selective protein degradation."
Zhen M., Heinlein R., Jones D., Jentsch S., Candido E.P.M.
Mol. Cell. Biol. 13:1371-1377(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"An essential ubiquitin-conjugating enzyme with tissue and developmental specificity in the nematode Caenorhabditis elegans."
Zhen M., Scheni J.E., Baillie D.L., Candido E.P.M.
EMBO J. 15:3229-3237(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION AS LET-70.
[3]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S56051 Genomic DNA. Translation: AAB25489.2.
Z68337 Genomic DNA. Translation: CAA92745.1.
PIRA48145.
T23820.
RefSeqNP_502065.1. NM_069664.4.
UniGeneCel.18192.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z2UX-ray1.10A1-147[»]
ProteinModelPortalP35129.
SMRP35129. Positions 1-147.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid43106. 5 interactions.
DIPDIP-24306N.
IntActP35129. 2 interactions.
MINTMINT-1108744.
STRING6239.M7.1.

Proteomic databases

PaxDbP35129.
PRIDEP35129.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaM7.1.1; M7.1.1; M7.1.
M7.1.2; M7.1.2; M7.1.
GeneID178006.
KEGGcel:CELE_M7.1.
UCSCM7.1. c. elegans.

Organism-specific databases

CTD178006.
WormBaseM7.1; CE03482; WBGene00002344; let-70.

Phylogenomic databases

eggNOGCOG5078.
HOGENOMHOG000233455.
InParanoidP35129.
KOK06689.
OMAIAHIYKQ.
PhylomeDBP35129.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP35129.
NextBio899334.

Entry information

Entry nameUBC2_CAEEL
AccessionPrimary (citable) accession number: P35129
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase