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Protein

Ubiquitin-conjugating enzyme E2 2

Gene

let-70

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin protein ligase binding Source: WormBase
  • ubiquitin-protein transferase activity Source: WormBase

GO - Biological processi

  • protein ubiquitination Source: WormBase
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: WormBase

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 2 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 2
Lethal protein 70
Ubiquitin carrier protein 2
Ubiquitin-protein ligase 2
Gene namesi
Name:let-70
Synonyms:ubc-2
ORF Names:M7.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiM7.1; CE03482; WBGene00002344; let-70.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825151 – 147Ubiquitin-conjugating enzyme E2 2Add BLAST147

Proteomic databases

EPDiP35129.
PaxDbiP35129.
PeptideAtlasiP35129.
PRIDEiP35129.

Expressioni

Gene expression databases

BgeeiWBGene00002344.

Interactioni

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: WormBase

Protein-protein interaction databases

BioGridi43106. 5 interactors.
DIPiDIP-24306N.
IntActiP35129. 2 interactors.
MINTiMINT-1108744.
STRINGi6239.M7.1.1.

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 15Combined sources15
Beta strandi19 – 26Combined sources8
Beta strandi29 – 38Combined sources10
Turni44 – 47Combined sources4
Beta strandi49 – 55Combined sources7
Turni58 – 61Combined sources4
Beta strandi66 – 71Combined sources6
Helixi87 – 89Combined sources3
Turni90 – 92Combined sources3
Helixi99 – 111Combined sources13
Helixi121 – 129Combined sources9
Helixi131 – 145Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z2UX-ray1.10A1-147[»]
ProteinModelPortaliP35129.
SMRiP35129.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35129.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00900000141035.
HOGENOMiHOG000233455.
InParanoidiP35129.
KOiK06689.
OMAiIAHIYKQ.
OrthoDBiEOG091G0GF8.
PhylomeDBiP35129.

Family and domain databases

CDDicd00195. UBCc. 1 hit.
Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P35129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKRIQKEL QDLGRDPPAQ CSAGPVGDDL FHWQATIMGP PESPYQGGVF
60 70 80 90 100
FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS
110 120 130 140
KVLLSICSLL CDPNPDDPLV PEIARIYKTD RERYNQLARE WTQKYAM
Length:147
Mass (Da):16,705
Last modified:February 1, 1994 - v1
Checksum:i9E91884D307F2120
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti75H → Y in let-70(S1132); which shows growth arrest in larval stage. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S56051 Genomic DNA. Translation: AAB25489.2.
Z68337 Genomic DNA. Translation: CAA92745.1.
PIRiA48145.
T23820.
RefSeqiNP_502065.1. NM_069664.4.
UniGeneiCel.18192.

Genome annotation databases

EnsemblMetazoaiM7.1; M7.1; WBGene00002344.
GeneIDi178006.
KEGGicel:CELE_M7.1.
UCSCiM7.1. c. elegans.

Similar proteinsi

Entry informationi

Entry nameiUBC2_CAEEL
AccessioniPrimary (citable) accession number: P35129
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 25, 2017
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families