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P35129

- UBC2_CAEEL

UniProt

P35129 - UBC2_CAEEL

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Protein

Ubiquitin-conjugating enzyme E2 2

Gene

let-70

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin protein ligase binding Source: WormBase
  4. ubiquitin-protein transferase activity Source: WormBase

GO - Biological processi

  1. protein ubiquitination Source: WormBase
  2. protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_107985. Signaling by BMP.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 2 (EC:6.3.2.19)
Alternative name(s):
Lethal protein 70
Ubiquitin carrier protein 2
Ubiquitin-protein ligase 2
Gene namesi
Name:let-70
Synonyms:ubc-2
ORF Names:M7.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome IV

Organism-specific databases

WormBaseiM7.1; CE03482; WBGene00002344; let-70.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 147147Ubiquitin-conjugating enzyme E2 2PRO_0000082515Add
BLAST

Proteomic databases

PaxDbiP35129.
PRIDEiP35129.

Interactioni

Protein-protein interaction databases

BioGridi43106. 5 interactions.
DIPiDIP-24306N.
IntActiP35129. 2 interactions.
MINTiMINT-1108744.
STRINGi6239.M7.1.

Structurei

Secondary structure

147
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1 – 1515
Beta strandi19 – 268
Beta strandi29 – 3810
Turni44 – 474
Beta strandi49 – 557
Turni58 – 614
Beta strandi66 – 716
Helixi87 – 893
Turni90 – 923
Helixi99 – 11113
Helixi121 – 1299
Helixi131 – 14515

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z2UX-ray1.10A1-147[»]
ProteinModelPortaliP35129.
SMRiP35129. Positions 1-147.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35129.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00760000119012.
HOGENOMiHOG000233455.
InParanoidiP35129.
KOiK06689.
OMAiNDLGRDP.
PhylomeDBiP35129.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35129-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALKRIQKEL QDLGRDPPAQ CSAGPVGDDL FHWQATIMGP PESPYQGGVF
60 70 80 90 100
FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS
110 120 130 140
KVLLSICSLL CDPNPDDPLV PEIARIYKTD RERYNQLARE WTQKYAM
Length:147
Mass (Da):16,705
Last modified:February 1, 1994 - v1
Checksum:i9E91884D307F2120
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti75 – 751H → Y in let-70(S1132); which shows growth arrest in larval stage.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S56051 Genomic DNA. Translation: AAB25489.2.
Z68337 Genomic DNA. Translation: CAA92745.1.
PIRiA48145.
T23820.
RefSeqiNP_502065.1. NM_069664.4.
UniGeneiCel.18192.

Genome annotation databases

EnsemblMetazoaiM7.1; M7.1; WBGene00002344.
GeneIDi178006.
KEGGicel:CELE_M7.1.
UCSCiM7.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S56051 Genomic DNA. Translation: AAB25489.2 .
Z68337 Genomic DNA. Translation: CAA92745.1 .
PIRi A48145.
T23820.
RefSeqi NP_502065.1. NM_069664.4.
UniGenei Cel.18192.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Z2U X-ray 1.10 A 1-147 [» ]
ProteinModelPortali P35129.
SMRi P35129. Positions 1-147.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 43106. 5 interactions.
DIPi DIP-24306N.
IntActi P35129. 2 interactions.
MINTi MINT-1108744.
STRINGi 6239.M7.1.

Proteomic databases

PaxDbi P35129.
PRIDEi P35129.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai M7.1 ; M7.1 ; WBGene00002344 .
GeneIDi 178006.
KEGGi cel:CELE_M7.1.
UCSCi M7.1. c. elegans.

Organism-specific databases

CTDi 178006.
WormBasei M7.1 ; CE03482 ; WBGene00002344 ; let-70.

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00760000119012.
HOGENOMi HOG000233455.
InParanoidi P35129.
KOi K06689.
OMAi NDLGRDP.
PhylomeDBi P35129.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_107985. Signaling by BMP.

Miscellaneous databases

EvolutionaryTracei P35129.
NextBioi 899334.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The ubc-2 gene of Caenorhabditis elegans encodes a ubiquitin-conjugating enzyme involved in selective protein degradation."
    Zhen M., Heinlein R., Jones D., Jentsch S., Candido E.P.M.
    Mol. Cell. Biol. 13:1371-1377(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "An essential ubiquitin-conjugating enzyme with tissue and developmental specificity in the nematode Caenorhabditis elegans."
    Zhen M., Scheni J.E., Baillie D.L., Candido E.P.M.
    EMBO J. 15:3229-3237(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION AS LET-70.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiUBC2_CAEEL
AccessioniPrimary (citable) accession number: P35129
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3