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P35129

- UBC2_CAEEL

UniProt

P35129 - UBC2_CAEEL

Protein

Ubiquitin-conjugating enzyme E2 2

Gene

let-70

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin protein ligase binding Source: WormBase
    4. ubiquitin-protein transferase activity Source: WormBase

    GO - Biological processi

    1. protein ubiquitination Source: WormBase
    2. protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: WormBase

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_107985. Signaling by BMP.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 2 (EC:6.3.2.19)
    Alternative name(s):
    Lethal protein 70
    Ubiquitin carrier protein 2
    Ubiquitin-protein ligase 2
    Gene namesi
    Name:let-70
    Synonyms:ubc-2
    ORF Names:M7.1
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome IV

    Organism-specific databases

    WormBaseiM7.1; CE03482; WBGene00002344; let-70.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 147147Ubiquitin-conjugating enzyme E2 2PRO_0000082515Add
    BLAST

    Proteomic databases

    PaxDbiP35129.
    PRIDEiP35129.

    Interactioni

    Protein-protein interaction databases

    BioGridi43106. 5 interactions.
    DIPiDIP-24306N.
    IntActiP35129. 2 interactions.
    MINTiMINT-1108744.
    STRINGi6239.M7.1.

    Structurei

    Secondary structure

    147
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi1 – 1515
    Beta strandi19 – 268
    Beta strandi29 – 3810
    Turni44 – 474
    Beta strandi49 – 557
    Turni58 – 614
    Beta strandi66 – 716
    Helixi87 – 893
    Turni90 – 923
    Helixi99 – 11113
    Helixi121 – 1299
    Helixi131 – 14515

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Z2UX-ray1.10A1-147[»]
    ProteinModelPortaliP35129.
    SMRiP35129. Positions 1-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP35129.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00740000115162.
    HOGENOMiHOG000233455.
    InParanoidiP35129.
    KOiK06689.
    OMAiNDLGRDP.
    PhylomeDBiP35129.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35129-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALKRIQKEL QDLGRDPPAQ CSAGPVGDDL FHWQATIMGP PESPYQGGVF    50
    FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS 100
    KVLLSICSLL CDPNPDDPLV PEIARIYKTD RERYNQLARE WTQKYAM 147
    Length:147
    Mass (Da):16,705
    Last modified:February 1, 1994 - v1
    Checksum:i9E91884D307F2120
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti75 – 751H → Y in let-70(S1132); which shows growth arrest in larval stage.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S56051 Genomic DNA. Translation: AAB25489.2.
    Z68337 Genomic DNA. Translation: CAA92745.1.
    PIRiA48145.
    T23820.
    RefSeqiNP_502065.1. NM_069664.4.
    UniGeneiCel.18192.

    Genome annotation databases

    EnsemblMetazoaiM7.1; M7.1; WBGene00002344.
    GeneIDi178006.
    KEGGicel:CELE_M7.1.
    UCSCiM7.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S56051 Genomic DNA. Translation: AAB25489.2 .
    Z68337 Genomic DNA. Translation: CAA92745.1 .
    PIRi A48145.
    T23820.
    RefSeqi NP_502065.1. NM_069664.4.
    UniGenei Cel.18192.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Z2U X-ray 1.10 A 1-147 [» ]
    ProteinModelPortali P35129.
    SMRi P35129. Positions 1-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 43106. 5 interactions.
    DIPi DIP-24306N.
    IntActi P35129. 2 interactions.
    MINTi MINT-1108744.
    STRINGi 6239.M7.1.

    Proteomic databases

    PaxDbi P35129.
    PRIDEi P35129.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai M7.1 ; M7.1 ; WBGene00002344 .
    GeneIDi 178006.
    KEGGi cel:CELE_M7.1.
    UCSCi M7.1. c. elegans.

    Organism-specific databases

    CTDi 178006.
    WormBasei M7.1 ; CE03482 ; WBGene00002344 ; let-70.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00740000115162.
    HOGENOMi HOG000233455.
    InParanoidi P35129.
    KOi K06689.
    OMAi NDLGRDP.
    PhylomeDBi P35129.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_107985. Signaling by BMP.

    Miscellaneous databases

    EvolutionaryTracei P35129.
    NextBioi 899334.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ubc-2 gene of Caenorhabditis elegans encodes a ubiquitin-conjugating enzyme involved in selective protein degradation."
      Zhen M., Heinlein R., Jones D., Jentsch S., Candido E.P.M.
      Mol. Cell. Biol. 13:1371-1377(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "An essential ubiquitin-conjugating enzyme with tissue and developmental specificity in the nematode Caenorhabditis elegans."
      Zhen M., Scheni J.E., Baillie D.L., Candido E.P.M.
      EMBO J. 15:3229-3237(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION AS LET-70.
    3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiUBC2_CAEEL
    AccessioniPrimary (citable) accession number: P35129
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3