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Protein

Ubiquitin-conjugating enzyme E2 N

Gene

ben

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins.

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin conjugating enzyme binding Source: FlyBase
  • ubiquitin protein ligase binding Source: FlyBase
  • ubiquitin-protein transferase activity Source: FlyBase

GO - Biological processi

  • axonogenesis Source: FlyBase
  • axon target recognition Source: FlyBase
  • flight behavior Source: FlyBase
  • grooming behavior Source: FlyBase
  • jump response Source: FlyBase
  • photoreceptor cell morphogenesis Source: FlyBase
  • positive regulation of cell death Source: FlyBase
  • positive regulation of JNK cascade Source: FlyBase
  • positive regulation of mitotic cell cycle, embryonic Source: FlyBase
  • positive regulation of protein polyubiquitination Source: FlyBase
  • postreplication repair Source: GO_Central
  • protein K63-linked ubiquitination Source: GO_Central
  • protein polyubiquitination Source: FlyBase
  • response to anesthetic Source: FlyBase
  • response to oxidative stress Source: FlyBase
  • synapse maturation Source: FlyBase
  • synaptic growth at neuromuscular junction Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-1169408. ISG15 antiviral mechanism.
R-DME-446652. Interleukin-1 signaling.
R-DME-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-DME-5696395. Formation of Incision Complex in GG-NER.
R-DME-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
R-DME-937039. IRAK1 recruits IKK complex.
R-DME-975110. TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling.
R-DME-975144. IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation.
SignaLinkiP35128.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 N (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme N
Protein bendless
Ubiquitin carrier protein N
Ubiquitin-conjugating enzyme E2-17 kDa
Ubiquitin-protein ligase D3
Ubiquitin-protein ligase N
Gene namesi
Name:ben
Synonyms:UbcD3
ORF Names:CG18319
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000173. ben.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: FlyBase
  • perinuclear region of cytoplasm Source: FlyBase
  • UBC13-UEV1A complex Source: FlyBase
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Mutants in this gene exhibit several, largely neuronal defects including lesions affecting the neuronal connectivity of the giant fiber with the "jumping muscle", and the axons of photoreceptor cells R7 and R8 fail to make the proper right-angle turn into the medulla (hence the term "bendless").1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825211 – 151Ubiquitin-conjugating enzyme E2 NAdd BLAST151

Proteomic databases

PaxDbiP35128.
PRIDEiP35128.

Expressioni

Gene expression databases

BgeeiFBgn0000173.
ExpressionAtlasiP35128. baseline.
GenevisibleiP35128. DM.

Interactioni

GO - Molecular functioni

  • ubiquitin conjugating enzyme binding Source: FlyBase
  • ubiquitin protein ligase binding Source: FlyBase

Protein-protein interaction databases

BioGridi58728. 4 interactors.
DIPiDIP-22866N.
MINTiMINT-1003000.
STRINGi7227.FBpp0073686.

Structurei

3D structure databases

ProteinModelPortaliP35128.
SMRiP35128.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00540000070023.
InParanoidiP35128.
KOiK10580.
OMAiYFDVEIH.
OrthoDBiEOG091G0VPD.
PhylomeDBiP35128.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35128-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLPRRIIK ETQRLMQEPV PGINAIPDEN NARYFHVIVT GPNDSPFEGG
60 70 80 90 100
VFKLELFLPE DYPMSAPKVR FITKIYHPNI DRLGRICLDV LKDKWSPALQ
110 120 130 140 150
IRTILLSIQA LLSAPNPDDP LANDVAELWK VNEAEAIRNA REWTQKYAVE

D
Length:151
Mass (Da):17,236
Last modified:February 1, 1994 - v1
Checksum:i1D096E72A7AEA420
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20126 mRNA. Translation: AAA28392.1.
S70118 mRNA. Translation: AAB30753.1.
EU217226 Genomic DNA. Translation: ABW92183.1.
EU217227 Genomic DNA. Translation: ABW92184.1.
EU217228 Genomic DNA. Translation: ABW92185.1.
EU217229 Genomic DNA. Translation: ABW92186.1.
EU217230 Genomic DNA. Translation: ABW92187.1.
EU217231 Genomic DNA. Translation: ABW92188.1.
EU217232 Genomic DNA. Translation: ABW92189.1.
EU217233 Genomic DNA. Translation: ABW92190.1.
EU217234 Genomic DNA. Translation: ABW92191.1.
EU217235 Genomic DNA. Translation: ABW92192.1.
EU217236 Genomic DNA. Translation: ABW92193.1.
EU217237 Genomic DNA. Translation: ABW92194.1.
AE014298 Genomic DNA. Translation: AAF48338.1.
AY069527 mRNA. Translation: AAL39672.1.
PIRiS35793.
RefSeqiNP_001162752.1. NM_001169281.2.
NP_001245663.1. NM_001258734.2.
NP_001259540.1. NM_001272611.2.
NP_001259541.1. NM_001272612.2.
NP_511150.1. NM_078595.3.
UniGeneiDm.2088.

Genome annotation databases

EnsemblMetazoaiFBtr0073855; FBpp0073686; FBgn0000173.
FBtr0300566; FBpp0289793; FBgn0000173.
FBtr0307296; FBpp0298297; FBgn0000173.
FBtr0332843; FBpp0305066; FBgn0000173.
FBtr0332844; FBpp0305067; FBgn0000173.
GeneIDi32358.
KEGGidme:Dmel_CG18319.
UCSCiCG18319-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20126 mRNA. Translation: AAA28392.1.
S70118 mRNA. Translation: AAB30753.1.
EU217226 Genomic DNA. Translation: ABW92183.1.
EU217227 Genomic DNA. Translation: ABW92184.1.
EU217228 Genomic DNA. Translation: ABW92185.1.
EU217229 Genomic DNA. Translation: ABW92186.1.
EU217230 Genomic DNA. Translation: ABW92187.1.
EU217231 Genomic DNA. Translation: ABW92188.1.
EU217232 Genomic DNA. Translation: ABW92189.1.
EU217233 Genomic DNA. Translation: ABW92190.1.
EU217234 Genomic DNA. Translation: ABW92191.1.
EU217235 Genomic DNA. Translation: ABW92192.1.
EU217236 Genomic DNA. Translation: ABW92193.1.
EU217237 Genomic DNA. Translation: ABW92194.1.
AE014298 Genomic DNA. Translation: AAF48338.1.
AY069527 mRNA. Translation: AAL39672.1.
PIRiS35793.
RefSeqiNP_001162752.1. NM_001169281.2.
NP_001245663.1. NM_001258734.2.
NP_001259540.1. NM_001272611.2.
NP_001259541.1. NM_001272612.2.
NP_511150.1. NM_078595.3.
UniGeneiDm.2088.

3D structure databases

ProteinModelPortaliP35128.
SMRiP35128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58728. 4 interactors.
DIPiDIP-22866N.
MINTiMINT-1003000.
STRINGi7227.FBpp0073686.

Proteomic databases

PaxDbiP35128.
PRIDEiP35128.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0073855; FBpp0073686; FBgn0000173.
FBtr0300566; FBpp0289793; FBgn0000173.
FBtr0307296; FBpp0298297; FBgn0000173.
FBtr0332843; FBpp0305066; FBgn0000173.
FBtr0332844; FBpp0305067; FBgn0000173.
GeneIDi32358.
KEGGidme:Dmel_CG18319.
UCSCiCG18319-RA. d. melanogaster.

Organism-specific databases

CTDi32358.
FlyBaseiFBgn0000173. ben.

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00540000070023.
InParanoidiP35128.
KOiK10580.
OMAiYFDVEIH.
OrthoDBiEOG091G0VPD.
PhylomeDBiP35128.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-DME-1169408. ISG15 antiviral mechanism.
R-DME-446652. Interleukin-1 signaling.
R-DME-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-DME-5696395. Formation of Incision Complex in GG-NER.
R-DME-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
R-DME-937039. IRAK1 recruits IKK complex.
R-DME-975110. TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling.
R-DME-975144. IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation.
SignaLinkiP35128.

Miscellaneous databases

GenomeRNAii32358.
PROiP35128.

Gene expression databases

BgeeiFBgn0000173.
ExpressionAtlasiP35128. baseline.
GenevisibleiP35128. DM.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBE2N_DROME
AccessioniPrimary (citable) accession number: P35128
Secondary accession number(s): A9YHJ7, Q9VY67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.