P35128 (UBE2N_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 N
EC=6.3.2.19

Alternative name(s):
Protein bendless
Ubiquitin carrier protein N
Ubiquitin-conjugating enzyme E2-17 kDa
Ubiquitin-protein ligase D3
Ubiquitin-protein ligase N

Gene names

Name:	ben
Synonyms:	UbcD3
ORF Names:	CG18319

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Disruption phenotype	Mutants in this gene exhibit several, largely neuronal defects including lesions affecting the neuronal connectivity of the giant fiber with the "jumping muscle", and the axons of photoreceptor cells R7 and R8 fail to make the proper right-angle turn into the medulla (hence the term "bendless"). Ref.1
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon target recognition Inferred from mutant phenotype Ref.1. Source: FlyBase flight behavior Inferred from mutant phenotype Ref.2. Source: FlyBase grooming behavior Inferred from mutant phenotype PubMed 8454205. Source: FlyBase jump response Inferred from mutant phenotype PubMed 6808394. Source: FlyBase photoreceptor cell morphogenesis Inferred from mutant phenotype Ref.1. Source: FlyBase response to anesthetic Inferred from mutant phenotype PubMed 19863272. Source: FlyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity Ref.2. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 151	151	Ubiquitin-conjugating enzyme E2 N		PRO_0000082521
Sites
	Active site	87	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35128 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 1D096E72A7AEA420	FASTA	151	17,236
	10 20 30 40 50 60 MSSLPRRIIK ETQRLMQEPV PGINAIPDEN NARYFHVIVT GPNDSPFEGG VFKLELFLPE 70 80 90 100 110 120 DYPMSAPKVR FITKIYHPNI DRLGRICLDV LKDKWSPALQ IRTILLSIQA LLSAPNPDDP 130 140 150 LANDVAELWK VNEAEAIRNA REWTQKYAVE D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila bendless gene encodes a neural protein related to ubiquitin-conjugating enzymes." Muralidhar M., Thomas J.B. Neuron 11:253-266(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE.
[2]	"Bendless, a Drosophila gene affecting neuronal connectivity, encodes a ubiquitin-conjugating enzyme homolog." Oh C.E., McMahon R., Benzer S., Tanouye M.A. J. Neurosci. 14:3166-3179(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Hitchhiking effects of recurrent beneficial amino acid substitutions in the Drosophila melanogaster genome." Andolfatto P. Genome Res. 17:1755-1762(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ZW104, ZW109, ZW122, ZW123, ZW133, ZW136, ZW139, ZW140, ZW141, ZW142, ZW143 and ZW144.
[4]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[5]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[6]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L20126 mRNA. Translation: AAA28392.1. S70118 mRNA. Translation: AAB30753.1. EU217226 Genomic DNA. Translation: ABW92183.1. EU217227 Genomic DNA. Translation: ABW92184.1. EU217228 Genomic DNA. Translation: ABW92185.1. EU217229 Genomic DNA. Translation: ABW92186.1. EU217230 Genomic DNA. Translation: ABW92187.1. EU217231 Genomic DNA. Translation: ABW92188.1. EU217232 Genomic DNA. Translation: ABW92189.1. EU217233 Genomic DNA. Translation: ABW92190.1. EU217234 Genomic DNA. Translation: ABW92191.1. EU217235 Genomic DNA. Translation: ABW92192.1. EU217236 Genomic DNA. Translation: ABW92193.1. EU217237 Genomic DNA. Translation: ABW92194.1. AE014298 Genomic DNA. Translation: AAF48338.1. AY069527 mRNA. Translation: AAL39672.1.
PIR	S35793.
RefSeq	NP_001162752.1. NM_001169281.1. NP_001245663.1. NM_001258734.1. NP_001259540.1. NM_001272611.1. NP_001259541.1. NM_001272612.1. NP_511150.1. NM_078595.2.
UniGene	Dm.2088.
3D structure databases
ProteinModelPortal	P35128.
SMR	P35128. Positions 4-149.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-22866N.
MINT	MINT-1003000.
STRING	7227.FBpp0289793.
Proteomic databases
PaxDb	P35128.
PRIDE	P35128.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0073855; FBpp0073686; FBgn0000173. FBtr0300566; FBpp0289793; FBgn0000173. FBtr0307296; FBpp0298297; FBgn0000173. FBtr0332843; FBpp0305066; FBgn0000173. FBtr0332844; FBpp0305067; FBgn0000173.
GeneID	32358.
KEGG	dme:Dmel_CG18319.
UCSC	CG18319-RA. d. melanogaster.
Organism-specific databases
CTD	32358.
FlyBase	FBgn0000173. ben.
Phylogenomic databases
eggNOG	COG5078.
GeneTree	ENSGT00540000070023.
InParanoid	P35128.
KO	K10580.
OMA	DDNLRYF.
OrthoDB	EOG402V8G.
PhylomeDB	P35128.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Bgee	P35128.
GermOnline	CG18319. Drosophila melanogaster.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GTF2IRD1. drosophila.
GenomeRNAi	32358.
NextBio	778098.

Entry information

Entry name

UBE2N_DROME

Accession

Primary (citable) accession number: P35128
Secondary accession number(s): A9YHJ7, Q9VY67

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project