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Protein

Ubiquitin-conjugating enzyme E2 N

Gene

ben

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins.

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin conjugating enzyme binding Source: FlyBase
  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin protein ligase binding Source: FlyBase
  • ubiquitin-protein transferase activity Source: FlyBase

GO - Biological processi

  • axonogenesis Source: FlyBase
  • axon target recognition Source: FlyBase
  • flight behavior Source: FlyBase
  • grooming behavior Source: FlyBase
  • jump response Source: FlyBase
  • peptidoglycan recognition protein signaling pathway Source: FlyBase
  • photoreceptor cell morphogenesis Source: FlyBase
  • positive regulation of cell death Source: FlyBase
  • positive regulation of JNK cascade Source: FlyBase
  • positive regulation of mitotic cell cycle, embryonic Source: FlyBase
  • positive regulation of protein polyubiquitination Source: FlyBase
  • postreplication repair Source: GO_Central
  • protein K63-linked ubiquitination Source: GO_Central
  • protein polyubiquitination Source: FlyBase
  • response to anesthetic Source: FlyBase
  • response to oxidative stress Source: FlyBase
  • synapse maturation Source: FlyBase
  • synaptic growth at neuromuscular junction Source: FlyBase

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-209447 Activation of the IkappaB kinase complex, KEY:IRD5 dimer:KEY
R-DME-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-DME-5693571 Nonhomologous End-Joining (NHEJ)
R-DME-5696395 Formation of Incision Complex in GG-NER
R-DME-9020702 Interleukin-1 signaling
R-DME-937039 IRAK1 recruits IKK complex
R-DME-975110 TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling
R-DME-975144 IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation
R-DME-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiP35128
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 N (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme N
Protein bendless
Ubiquitin carrier protein N
Ubiquitin-conjugating enzyme E2-17 kDa
Ubiquitin-protein ligase D3
Ubiquitin-protein ligase N
Gene namesi
Name:ben
Synonyms:UbcD3
ORF Names:CG18319
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000173 ben

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Mutants in this gene exhibit several, largely neuronal defects including lesions affecting the neuronal connectivity of the giant fiber with the "jumping muscle", and the axons of photoreceptor cells R7 and R8 fail to make the proper right-angle turn into the medulla (hence the term "bendless").1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825211 – 151Ubiquitin-conjugating enzyme E2 NAdd BLAST151

Proteomic databases

PaxDbiP35128
PRIDEiP35128

Expressioni

Gene expression databases

BgeeiFBgn0000173
ExpressionAtlasiP35128 baseline and differential
GenevisibleiP35128 DM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Uev1AQ9VRL14EBI-101550,EBI-192329

GO - Molecular functioni

  • ubiquitin conjugating enzyme binding Source: FlyBase
  • ubiquitin protein ligase binding Source: FlyBase

Protein-protein interaction databases

BioGridi58728, 12 interactors
DIPiDIP-22866N
IntActiP35128, 3 interactors
STRINGi7227.FBpp0073686

Structurei

3D structure databases

ProteinModelPortaliP35128
SMRiP35128
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00540000070023
InParanoidiP35128
KOiK10580
OMAiYFDVTIQ
OrthoDBiEOG091G0VPD
PhylomeDBiP35128

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P35128-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLPRRIIK ETQRLMQEPV PGINAIPDEN NARYFHVIVT GPNDSPFEGG
60 70 80 90 100
VFKLELFLPE DYPMSAPKVR FITKIYHPNI DRLGRICLDV LKDKWSPALQ
110 120 130 140 150
IRTILLSIQA LLSAPNPDDP LANDVAELWK VNEAEAIRNA REWTQKYAVE

D
Length:151
Mass (Da):17,236
Last modified:February 1, 1994 - v1
Checksum:i1D096E72A7AEA420
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20126 mRNA Translation: AAA28392.1
S70118 mRNA Translation: AAB30753.1
EU217226 Genomic DNA Translation: ABW92183.1
EU217227 Genomic DNA Translation: ABW92184.1
EU217228 Genomic DNA Translation: ABW92185.1
EU217229 Genomic DNA Translation: ABW92186.1
EU217230 Genomic DNA Translation: ABW92187.1
EU217231 Genomic DNA Translation: ABW92188.1
EU217232 Genomic DNA Translation: ABW92189.1
EU217233 Genomic DNA Translation: ABW92190.1
EU217234 Genomic DNA Translation: ABW92191.1
EU217235 Genomic DNA Translation: ABW92192.1
EU217236 Genomic DNA Translation: ABW92193.1
EU217237 Genomic DNA Translation: ABW92194.1
AE014298 Genomic DNA Translation: AAF48338.1
AY069527 mRNA Translation: AAL39672.1
PIRiS35793
RefSeqiNP_001162752.1, NM_001169281.2
NP_001245663.1, NM_001258734.2
NP_001259540.1, NM_001272611.2
NP_001259541.1, NM_001272612.2
NP_511150.1, NM_078595.3
UniGeneiDm.2088

Genome annotation databases

EnsemblMetazoaiFBtr0073855; FBpp0073686; FBgn0000173
FBtr0300566; FBpp0289793; FBgn0000173
FBtr0307296; FBpp0298297; FBgn0000173
FBtr0332843; FBpp0305066; FBgn0000173
FBtr0332844; FBpp0305067; FBgn0000173
GeneIDi32358
KEGGidme:Dmel_CG18319
UCSCiCG18319-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiUBE2N_DROME
AccessioniPrimary (citable) accession number: P35128
Secondary accession number(s): A9YHJ7, Q9VY67
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 23, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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