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P35128 (UBE2N_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 N

EC=6.3.2.19
Alternative name(s):
Protein bendless
Ubiquitin carrier protein N
Ubiquitin-conjugating enzyme E2-17 kDa
Ubiquitin-protein ligase D3
Ubiquitin-protein ligase N
Gene names
Name:ben
Synonyms:UbcD3
ORF Names:CG18319
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length151 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Disruption phenotype

Mutants in this gene exhibit several, largely neuronal defects including lesions affecting the neuronal connectivity of the giant fiber with the "jumping muscle", and the axons of photoreceptor cells R7 and R8 fail to make the proper right-angle turn into the medulla (hence the term "bendless"). Ref.1

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaxon target recognition

Inferred from mutant phenotype Ref.1. Source: FlyBase

axonogenesis

Inferred from mutant phenotype PubMed 6808394. Source: FlyBase

flight behavior

Inferred from mutant phenotype Ref.2. Source: FlyBase

grooming behavior

Inferred from mutant phenotype PubMed 8454205. Source: FlyBase

jump response

Inferred from mutant phenotype PubMed 6808394. Source: FlyBase

photoreceptor cell morphogenesis

Inferred from mutant phenotype Ref.1. Source: FlyBase

positive regulation of mitotic cell cycle, embryonic

Inferred from mutant phenotype PubMed 19141674. Source: FlyBase

response to anesthetic

Inferred from mutant phenotype PubMed 19863272. Source: FlyBase

synapse maturation

Inferred from mutant phenotype PubMed 18716220. Source: FlyBase

synaptic growth at neuromuscular junction

Inferred from mutant phenotype PubMed 18716220. Source: FlyBase

   Cellular_componentUBC13-UEV1A complex

Inferred from direct assay PubMed 19141674. Source: FlyBase

nucleus

Inferred from direct assay PubMed 19141674. Source: FlyBase

perinuclear region of cytoplasm

Inferred from direct assay PubMed 19141674. Source: FlyBase

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 19141674. Source: FlyBase

ubiquitin protein ligase binding

Inferred from physical interaction PubMed 19141674. Source: FlyBase

ubiquitin-protein transferase activity

Inferred from sequence or structural similarity Ref.2. Source: FlyBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 151151Ubiquitin-conjugating enzyme E2 N
PRO_0000082521

Sites

Active site871Glycyl thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
P35128 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 1D096E72A7AEA420

FASTA15117,236
        10         20         30         40         50         60 
MSSLPRRIIK ETQRLMQEPV PGINAIPDEN NARYFHVIVT GPNDSPFEGG VFKLELFLPE 

        70         80         90        100        110        120 
DYPMSAPKVR FITKIYHPNI DRLGRICLDV LKDKWSPALQ IRTILLSIQA LLSAPNPDDP 

       130        140        150 
LANDVAELWK VNEAEAIRNA REWTQKYAVE D 

« Hide

References

« Hide 'large scale' references
[1]"The Drosophila bendless gene encodes a neural protein related to ubiquitin-conjugating enzymes."
Muralidhar M., Thomas J.B.
Neuron 11:253-266(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE.
[2]"Bendless, a Drosophila gene affecting neuronal connectivity, encodes a ubiquitin-conjugating enzyme homolog."
Oh C.E., McMahon R., Benzer S., Tanouye M.A.
J. Neurosci. 14:3166-3179(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Hitchhiking effects of recurrent beneficial amino acid substitutions in the Drosophila melanogaster genome."
Andolfatto P.
Genome Res. 17:1755-1762(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ZW104, ZW109, ZW122, ZW123, ZW133, ZW136, ZW139, ZW140, ZW141, ZW142, ZW143 and ZW144.
[4]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[5]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[6]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L20126 mRNA. Translation: AAA28392.1.
S70118 mRNA. Translation: AAB30753.1.
EU217226 Genomic DNA. Translation: ABW92183.1.
EU217227 Genomic DNA. Translation: ABW92184.1.
EU217228 Genomic DNA. Translation: ABW92185.1.
EU217229 Genomic DNA. Translation: ABW92186.1.
EU217230 Genomic DNA. Translation: ABW92187.1.
EU217231 Genomic DNA. Translation: ABW92188.1.
EU217232 Genomic DNA. Translation: ABW92189.1.
EU217233 Genomic DNA. Translation: ABW92190.1.
EU217234 Genomic DNA. Translation: ABW92191.1.
EU217235 Genomic DNA. Translation: ABW92192.1.
EU217236 Genomic DNA. Translation: ABW92193.1.
EU217237 Genomic DNA. Translation: ABW92194.1.
AE014298 Genomic DNA. Translation: AAF48338.1.
AY069527 mRNA. Translation: AAL39672.1.
PIRS35793.
RefSeqNP_001162752.1. NM_001169281.1.
NP_001245663.1. NM_001258734.1.
NP_001259540.1. NM_001272611.1.
NP_001259541.1. NM_001272612.1.
NP_511150.1. NM_078595.2.
UniGeneDm.2088.

3D structure databases

ProteinModelPortalP35128.
SMRP35128. Positions 4-149.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid58728. 4 interactions.
DIPDIP-22866N.
MINTMINT-1003000.
STRING7227.FBpp0289793.

Proteomic databases

PaxDbP35128.
PRIDEP35128.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0073855; FBpp0073686; FBgn0000173.
FBtr0300566; FBpp0289793; FBgn0000173.
FBtr0307296; FBpp0298297; FBgn0000173.
FBtr0332843; FBpp0305066; FBgn0000173.
FBtr0332844; FBpp0305067; FBgn0000173.
GeneID32358.
KEGGdme:Dmel_CG18319.
UCSCCG18319-RA. d. melanogaster.

Organism-specific databases

CTD32358.
FlyBaseFBgn0000173. ben.

Phylogenomic databases

eggNOGCOG5078.
GeneTreeENSGT00540000070023.
InParanoidP35128.
KOK10580.
OMADVAKHYK.
OrthoDBEOG7XWPQB.
PhylomeDBP35128.

Enzyme and pathway databases

SignaLinkP35128.
UniPathwayUPA00143.

Gene expression databases

BgeeP35128.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGTF2IRD1. drosophila.
GenomeRNAi32358.
NextBio778098.

Entry information

Entry nameUBE2N_DROME
AccessionPrimary (citable) accession number: P35128
Secondary accession number(s): A9YHJ7, Q9VY67
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 9, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase