Ubiquitin carboxyl-terminal hydrolase YUH1 - Saccharomyces cerevisiae (Baker's yeast)

Contribute

Send feedback

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P35127 (UBL1_YEAST)

Last modified June 16, 2009. Version 84. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Ubiquitin carboxyl-terminal hydrolase YUH1
    EC=3.4.19.12
Alternative name(s):
    Ubiquitin thioesterase

Gene names

Name:	YUH1
Ordered Locus Names:	YJR099W
ORF Names:	J1941

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Hide | Top

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C12 family.

Hide | Top

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred by curator. Source: SGD
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 236

236

Ubiquitin carboxyl-terminal hydrolase YUH1

PRO_0000211073

Regions

Region

43 – 60

Ubiquitin binding 1 Potential

Region

175 – 183

Ubiquitin binding 2 Potential

Sites

Active site

Nucleophile Ref.4

Active site

166

Proton donor Ref.4

Active site

181

Proton donor Ref.4

Site

Transition state stabilizer

Amino acid modifications

Modified residue

186

Phosphoserine Ref.3

Secondary structure

236

Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

P35127-1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: D239FEE25798B395
Show »

FASTA

236

26,385

        10         20         30         40         50         60 
MSGENRAVVP IESNPEVFTN FAHKLGLKNE WAYFDIYSLT EPELLAFLPR PVKAIVLLFP 

        70         80         90        100        110        120 
INEDRKSSTS QQITSSYDVI WFKQSVKNAC GLYAILHSLS NNQSLLEPGS DLDNFLKSQS 

       130        140        150        160        170        180 
DTSSSKNRFD DVTTDQFVLN VIKENVQTFS TGQSEAPEAT ADTNLHYITY VEENGGIFEL 

       190        200        210        220        230 
DGRNLSGPLY LGKSDPTATD LIEQELVRVR VASYMENANE EDVLNFAMLG LGPNWE

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a yeast ubiquitin-protein cleaving activity in Escherichia coli." Miller H.I., Henzel W.J., Ridgway J.B., Kuang W.-J., Chisholm V., Liu C.-C. Biotechnology (N.Y.) 7:698-704(1989) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, MASS SPECTROMETRY.
[4]	"Structural basis for the specificity of ubiquitin C-terminal hydrolases." Johnston S.C., Riddle S.M., Cohen R.E., Hill C.P. EMBO J. 18:3877-3887(1999) [PubMed: 10406793] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) IN COMPLEX WITH UBIQUITIN, ACTIVE SITES.
+	Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

Z49599 Genomic DNA. Translation: CAA89629.1.

PIR

S51332.

RefSeq

NP_012633.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CMX	X-ray	2.25	A/C	1-235	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:5052N.

IntAct

P35127. 3 interactions.

Protein family/group databases

MEROPS

C12.002.

Genome annotation databases

Ensembl

YJR099W. Saccharomyces cerevisiae. [Contig view]

GeneID

853562.

GenomeReviews

Gene locus YJR099W in contig Y13136_GR.

KEGG

sce:YJR099W.

NMPDR

fig|4932.3.peg.3607.

Organism-specific databases

CYGD

YJR099w.

SGD

S000003860. YUH1.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

P35127.

OMA

P35127. GRCVELD.

Enzyme and pathway databases

BRENDA

3.4.19.12. 250.

Gene expression databases

ArrayExpress

P35127.

GermOnline

YJR099W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001578. Peptidase_C12.
[Graphical view]

Gene3D

G3DSA:3.40.532.10. Peptidase_C12. 1 hit.

PANTHER

PTHR10589. Peptidase_C12. 1 hit.

Pfam

PF01088. Peptidase_C12. 1 hit.
[Graphical view]

PRINTS

PR00707. UBCTHYDRLASE.

ProDom

PD350662. Peptidase_C12. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00140. UCH_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

974317.

Hide | Top

Entry information

Entry name

UBL1_YEAST

Accession

Primary (citable) accession number: P35127

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

Hide | Top