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P35122

- UCHL_DROME

UniProt

P35122 - UCHL_DROME

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Protein

Ubiquitin carboxyl-terminal hydrolase

Gene

Uch

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei93 – 931NucleophilePROSITE-ProRule annotation
Active sitei164 – 1641Proton donorPROSITE-ProRule annotation
Sitei179 – 1791Important for enzyme activityBy similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: FlyBase

GO - Biological processi

  1. protein deubiquitination Source: FlyBase
  2. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC12.008.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase (EC:3.4.19.12)
Alternative name(s):
Ubiquitin thioesterase
Gene namesi
Name:Uch
Synonyms:ubc-D
ORF Names:CG4265
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0010288. Uch.

Subcellular locationi

GO - Cellular componenti

  1. microtubule associated complex Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Ubiquitin carboxyl-terminal hydrolasePRO_0000211072Add
BLAST

Proteomic databases

PaxDbiP35122.

Expressioni

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiP35122.
ExpressionAtlasiP35122. differential.

Interactioni

Protein-protein interaction databases

BioGridi59626. 63 interactions.
MINTiMINT-1599795.

Structurei

3D structure databases

ProteinModelPortaliP35122.
SMRiP35122. Positions 4-225.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

eggNOGiNOG327708.
GeneTreeiENSGT00510000046640.
InParanoidiP35122.
KOiK05609.
OMAiYVCFVKG.
OrthoDBiEOG7S7SFK.
PhylomeDBiP35122.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
PROSITEiPS00140. UCH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35122-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLTWTPLESN PEVLTKYIHK LGVSPAWSVT DVIGLEDDTL EWIPRPVKAF
60 70 80 90 100
ILLFPCSETY EKHRAEEHDR IKEVEEQHPE DLFYMRQFTH NACGTVALIH
110 120 130 140 150
SVANNKEVDI DRGVLKDFLE KTASLSPEER GRALEKDEKF TADHEALAQE
160 170 180 190 200
GQTNAANHEK VIHHFIALVN KEGTLYELDG RKSFPIKHGP TSEETFVKDA
210 220
AKVCKEFMAR DPNEVRFTVL ALTAAQQ
Length:227
Mass (Da):25,850
Last modified:August 30, 2005 - v2
Checksum:i298A025569C3D409
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221G → A in AAA15411. (PubMed:7683284)Curated
Sequence conflicti22 – 221G → A in CAA49358. (PubMed:7683284)Curated
Sequence conflicti22 – 221G → A in CAA49359. (PubMed:7683284)Curated
Sequence conflicti65 – 651A → T(PubMed:1426603)Curated
Sequence conflicti65 – 651A → T(PubMed:7683284)Curated
Sequence conflicti107 – 1071E → G(PubMed:1426603)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S60346 Genomic DNA. Translation: AAA15411.1.
X69678 mRNA. Translation: CAA49358.1.
X69679 Genomic DNA. Translation: CAA49359.1.
AE014134 Genomic DNA. Translation: AAF51291.1.
AF145600 mRNA. Translation: AAD38575.1.
PIRiS33956.
RefSeqiNP_001188681.1. NM_001201752.2.
NP_476940.1. NM_057592.4.
UniGeneiDm.4794.

Genome annotation databases

EnsemblMetazoaiFBtr0077844; FBpp0077516; FBgn0010288.
FBtr0303459; FBpp0292511; FBgn0010288.
GeneIDi33397.
KEGGidme:Dmel_CG4265.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S60346 Genomic DNA. Translation: AAA15411.1 .
X69678 mRNA. Translation: CAA49358.1 .
X69679 Genomic DNA. Translation: CAA49359.1 .
AE014134 Genomic DNA. Translation: AAF51291.1 .
AF145600 mRNA. Translation: AAD38575.1 .
PIRi S33956.
RefSeqi NP_001188681.1. NM_001201752.2.
NP_476940.1. NM_057592.4.
UniGenei Dm.4794.

3D structure databases

ProteinModelPortali P35122.
SMRi P35122. Positions 4-225.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 59626. 63 interactions.
MINTi MINT-1599795.

Protein family/group databases

MEROPSi C12.008.

Proteomic databases

PaxDbi P35122.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0077844 ; FBpp0077516 ; FBgn0010288 .
FBtr0303459 ; FBpp0292511 ; FBgn0010288 .
GeneIDi 33397.
KEGGi dme:Dmel_CG4265.

Organism-specific databases

CTDi 33397.
FlyBasei FBgn0010288. Uch.

Phylogenomic databases

eggNOGi NOG327708.
GeneTreei ENSGT00510000046640.
InParanoidi P35122.
KOi K05609.
OMAi YVCFVKG.
OrthoDBi EOG7S7SFK.
PhylomeDBi P35122.

Miscellaneous databases

GenomeRNAii 33397.
NextBioi 783351.

Gene expression databases

Bgeei P35122.
ExpressionAtlasi P35122. differential.

Family and domain databases

Gene3Di 3.40.532.10. 1 hit.
InterProi IPR001578. Peptidase_C12_UCH.
[Graphical view ]
PANTHERi PTHR10589. PTHR10589. 1 hit.
Pfami PF01088. Peptidase_C12. 1 hit.
[Graphical view ]
PRINTSi PR00707. UBCTHYDRLASE.
PROSITEi PS00140. UCH_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Comparisons of neuronal (PGP 9.5) and non-neuronal ubiquitin C-terminal hydrolases."
    Wilkinson K.D., Deshpande S., Larsen C.N.
    Biochem. Soc. Trans. 20:631-637(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning and analysis of expression of a ubiquitin carboxyl terminal hydrolase expressed during oogenesis in Drosophila melanogaster."
    Zhang N., Wilkinson K., Bownes M.
    Dev. Biol. 157:214-223(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DEVELOPMENTAL STAGE.
    Strain: Oregon-R.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Head.

Entry informationi

Entry nameiUCHL_DROME
AccessioniPrimary (citable) accession number: P35122
Secondary accession number(s): Q4PIX7, Q9TWA0, Q9V417
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: August 30, 2005
Last modified: October 29, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3