P35100 (CLPC_PEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Chaperone protein ClpC, chloroplastic Alternative name(s): ATP-dependent Clp protease ATP-binding subunit ClpC homolog Casein lytic proteinase C
Organism	Pisum sativum (Garden pea)
Taxonomic identifier	3888 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	922 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Molecular chaperone tha may interact with a clpP-like protease involved in degradation of denatured proteins in the chloroplast. Ref.2
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the clpA/clpB family. ClpC subfamily. Contains 1 UVR domain.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Repeat Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Gene Ontology (GO)
Biological_process	protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 72	72	Chloroplast Potential
	Chain	73 – 922	850	Chaperone protein ClpC, chloroplastic		PRO_0000005504
Regions
	Domain	509 – 544	36	UVR
	Nucleotide binding	300 – 307	8	ATP By similarity
	Nucleotide binding	643 – 650	8	ATP By similarity
	Region	255 – 502	248	I
	Region	569 – 760	192	II

Sequences

	Sequence		Length	Mass (Da)
	P35100 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 1CA4C1BA7D453DFE	FASTA	922	102,711
	10 20 30 40 50 60 MARVLAQSLS VPGLVAGHKD SQHKGSGKSK RSVKTMCALR TSGLRMSGFS GLRTFNHLNT 70 80 90 100 110 120 MMRPGLDFHS KVSKAVSSRR ARAKRFIPRA MFERFTEKAI KVIMLAQEEA RRLGHNFVGT 130 140 150 160 170 180 EQILLGLIGE GTGIAAKVLK SMGINLKDAR VEVEKIIGRG SGFVAVEIPF TPRAKRVLEL 190 200 210 220 230 240 SQEEARQLGH NYIGSEHLLL GLLREGEGVA ARVLENLGAD PTNIRTQVIR MVGESADSVT 250 260 270 280 290 300 ATVGSGSSNN KTPTLEEYGT NLTKLAEEGK LDPVVGRQPQ IERVTQILGR RTKNNPCLIG 310 320 330 340 350 360 EPGVGKTAIA EGLAQRIANG DVPETIEGKK VITLDMGLLV AGTKYRGEFE ERLKKLMEEI 370 380 390 400 410 420 KQSDDIILFI DEVHTLIGAG AAEGAIDAAN ILKPALARGE LQCIGATTLD EYRKHIEKDP 430 440 450 460 470 480 DLERRFQPVK VPEPTVDETI QILKGLRERY EIHHKLRYTD EALIAAAQLS YQYISDRFLP 490 500 510 520 530 540 DKAIDLVDEA GSRVRLQHAQ LPEEAKELDK EVRKIVKEKE EYVRNQDFEK AGELRDKEMD 550 560 570 580 590 600 LKAQISALIE KGKEMSKAET ETADEGPIVT EVDIQHIVSS WTGIPVDKVS ADESDRLLKM 610 620 630 640 650 660 EDTLHKRIIG QDEAVQAISR AIRRARVGLK NPNRPIASFI FSGPTGVGKS ELAKALAAYY 670 680 690 700 710 720 FGSEEAMIRL DMSEFMERHT VSKLIGSPPG YVGYTEGGQL TEAVRRRPYT VVLFDEIEKA 730 740 750 760 770 780 HPDVFNMMLQ ILEDGRLTDS KGRTVDFKNT LLIMTSNVGS SVIEKGGRRI GFDLDYDEKD 790 800 810 820 830 840 SSYNRIKSLV TEELKQYFRP EFLNRLDEMI VFRQLTKLEV KEIADIMLKE VFQRLKTKEI 850 860 870 880 890 900 ELQVTERFRD RVVDEGYNPS YGARPLRRAI MRLLEDSMAE KMLAREIKEG DSVIVDVDSD 910 920 GKVIVLNGSS GTPESLPEAL SI

References

[1]	"Characterization of a cDNA clone encoding a chloroplast-targeted Clp homologue." Moore T., Keegstra K. Plant Mol. Biol. 21:525-537(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Little Marvel.
[2]	"The Clp proteins: proteolysis regulators or molecular chaperones?" Squires C., Squires C.L. J. Bacteriol. 174:1081-1085(1992) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CLASSIFICATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L09547 mRNA. Translation: AAA33680.1.
PIR	S31164.
3D structure databases
ProteinModelPortal	P35100.
SMR	P35100. Positions 255-444.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-729N.
IntAct	P35100. 2 interactions.
Proteomic databases
PRIDE	P35100.
ProMEX	P35100.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.1780.10. 1 hit.
InterPro	IPR003593. AAA+_ATPase. IPR013093. ATPase_AAA-2. IPR003959. ATPase_AAA_core. IPR018368. Chaperonin_ClpA/B_CS. IPR001270. Chaprnin_ClpA/B. IPR019489. Clp_ATPase_C. IPR004176. Clp_N. IPR023150. Dbl_Clp-N. IPR027417. P-loop_NTPase. IPR001943. UVR_dom. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF07724. AAA_2. 1 hit. PF02861. Clp_N. 2 hits. PF10431. ClpB_D2-small. 1 hit. PF02151. UVR. 1 hit. [Graphical view]
PRINTS	PR00300. CLPPROTEASEA.
SMART	SM00382. AAA. 2 hits. SM01086. ClpB_D2-small. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 2 hits.
PROSITE	PS00870. CLPAB_1. 1 hit. PS00871. CLPAB_2. 1 hit. PS50151. UVR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPC_PEA

Accession

Primary (citable) accession number: P35100

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 29, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program