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Protein

Profilin-1

Gene

PRO1

Organism
Phleum pratense (Common timothy)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Profilin-1
Alternative name(s):
Allergen Phl p 11
Pollen allergen Phl p 12
Allergen: Phl p 12
Gene namesi
Name:PRO1
Synonyms:PHLPXI
OrganismiPhleum pratense (Common timothy)
Taxonomic identifieri15957 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaePoodaePoeaePoeae Chloroplast Group 2 (Poeae type)PoinaePhleum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3416. Phl p 12.0101.
553. Phl p 12.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 131130Profilin-1PRO_0000199663Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi13 ↔ 1151 Publication
Modified residuei111 – 1111PhosphothreonineBy similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Interactioni

Subunit structurei

Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio.

Structurei

3D structure databases

ProteinModelPortaliP35079.
SMRiP35079. Positions 2-131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi81 – 9717Involved in PIP2 interactionAdd
BLAST

Sequence similaritiesi

Belongs to the profilin family.Curated

Family and domain databases

InterProiIPR005455. PFN.
IPR027310. Profilin_CS.
[Graphical view]
PANTHERiPTHR11604. PTHR11604. 1 hit.
PfamiPF00235. Profilin. 1 hit.
[Graphical view]
PRINTSiPR00392. PROFILIN.
PR01640. PROFILINPLNT.
SMARTiSM00392. PROF. 1 hit.
[Graphical view]
SUPFAMiSSF55770. SSF55770. 1 hit.
PROSITEiPS00414. PROFILIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35079-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWQTYVDEH LMCEIEGHHL ASAAILGHDG TVWAQSADFP QFKPEEITGI
60 70 80 90 100
MKDFDEPGHL APTGMFVAGA KYMVIQGEPG RVIRGKKGAG GITIKKTGQA
110 120 130
LVVGIYDEPM TPGQCNMVVE RLGDYLVEQG M
Length:131
Mass (Da):14,235
Last modified:February 1, 1994 - v1
Checksum:i1B97A7FCD339714C
GO

Polymorphismi

Several isoforms of the allergen exist due to polymorphism.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77583 mRNA. Translation: CAA54686.1.
PIRiJC2080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77583 mRNA. Translation: CAA54686.1.
PIRiJC2080.

3D structure databases

ProteinModelPortaliP35079.
SMRiP35079. Positions 2-131.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3416. Phl p 12.0101.
553. Phl p 12.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR005455. PFN.
IPR027310. Profilin_CS.
[Graphical view]
PANTHERiPTHR11604. PTHR11604. 1 hit.
PfamiPF00235. Profilin. 1 hit.
[Graphical view]
PRINTSiPR00392. PROFILIN.
PR01640. PROFILINPLNT.
SMARTiSM00392. PROF. 1 hit.
[Graphical view]
SUPFAMiSSF55770. SSF55770. 1 hit.
PROSITEiPS00414. PROFILIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning and expression of timothy grass (Phleum pratense) pollen profilin in Escherichia coli: comparison with birch pollen profilin."
    Valenta R., Ball T., Vrtala S., Duchene M., Kraft D., Scheiner O.
    Biochem. Biophys. Res. Commun. 199:106-118(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pollen.
  2. "Analysis of the effects of polymorphism on pollen profilin structural functionality and the generation of conformational, T- and B-cell epitopes."
    Jimenez-Lopez J.C., Rodriguez-Garcia M.I., Alche J.D.
    PLoS ONE 8:E76066-E76066(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING, DISULFIDE BOND.

Entry informationi

Entry nameiPROF1_PHLPR
AccessioniPrimary (citable) accession number: P35079
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 17, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The variability of the residues taking part of IgE-binding epitopes might be responsible of the difference in cross-reactivity among olive pollen cultivars, and between distantly related pollen species, leading to a variable range of allergy reactions among atopic patients.1 Publication

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.