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Protein

Peroxisomal targeting signal receptor

Gene

PEX5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import.

GO - Molecular functioni

  • peroxisome matrix targeting signal-1 binding Source: SGD
  • protein binding, bridging Source: SGD

GO - Biological processi

  • protein import into peroxisome matrix, docking Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29817-MONOMER.

Protein family/group databases

TCDBi3.A.20.1.5. the peroxisomal protein importer (ppi) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal targeting signal receptor
Short name:
PTS1 receptor
Short name:
PTS1R
Alternative name(s):
Peroxin-5
Peroxisomal protein PAS10
Gene namesi
Name:PEX5
Synonyms:PAS10
Ordered Locus Names:YDR244W
ORF Names:YD8419.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR244W.
SGDiS000002652. PEX5.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • peroxisomal importomer complex Source: SGD
  • peroxisomal membrane Source: SGD
  • peroxisome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi6C → R: Loss of UBE2D2-independent ubiquitination. 1 Publication1
Mutagenesisi18K → R: Loss of UBE2D2-dependent ubiquitination. No effect on its function. 1 Publication1
Mutagenesisi24K → R: Loss of UBE2D2-dependent ubiquitination. No effect on its function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001063161 – 612Peroxisomal targeting signal receptorAdd BLAST612

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Cross-linki6Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki18Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki24Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei61PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitination at Cys-6 is UBC4-independent but requires the presence of PEX4. Ubiquitination at Lys-18 and Lys-24 are UBC4-dependent.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Thioester bond, Ubl conjugation

Proteomic databases

MaxQBiP35056.
PRIDEiP35056.

PTM databases

iPTMnetiP35056.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PEX13P806676EBI-13170,EBI-13206
PEX14P531128EBI-13170,EBI-13212

GO - Molecular functioni

  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi32295. 78 interactors.
DIPiDIP-2475N.
IntActiP35056. 15 interactors.
MINTiMINT-433689.

Structurei

3D structure databases

ProteinModelPortaliP35056.
SMRiP35056.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati64 – 97TPR 1Add BLAST34
Repeati313 – 346TPR 2Add BLAST34
Repeati347 – 380TPR 3Add BLAST34
Repeati381 – 418TPR 4Add BLAST38
Repeati419 – 456TPR 5Add BLAST38
Repeati457 – 490TPR 6Add BLAST34
Repeati491 – 524TPR 7Add BLAST34
Repeati525 – 558TPR 8Add BLAST34

Sequence similaritiesi

Contains 8 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

GeneTreeiENSGT00390000013941.
HOGENOMiHOG000158146.
InParanoidiP35056.
KOiK13342.
OMAiERWISTK.
OrthoDBiEOG092C1NSE.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR024111. PTS1R_family.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10130. PTHR10130. 1 hit.
PfamiPF00515. TPR_1. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50005. TPR. 4 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35056-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVGSCSVGN NPLAQLHKHT QQNKSLQFNQ KNNGRLNESP LQGTNKPGIS
60 70 80 90 100
EAFISNVNAI SQENMANMQR FINGEPLIDD KRRMEIGPSS GRLPPFSNVH
110 120 130 140 150
SLQTSANPTQ IKGVNDISHW SQEFQGSNSI QNRNADTGNS EKAWQRGSTT
160 170 180 190 200
ASSRFQYPNT MMNNYAYASM NSLSGSRLQS PAFMNQQQSG RSKEGVNEQE
210 220 230 240 250
QQPWTDQFEK LEKEVSENLD INDEIEKEEN VSEVEQNKPE TVEKEEGVYG
260 270 280 290 300
DQYQSDFQEV WDSIHKDAEE VLPSELVNDD LNLGEDYLKY LGGRVNGNIE
310 320 330 340 350
YAFQSNNEYF NNPNAYKIGC LLMENGAKLS EAALAFEAAV KEKPDHVDAW
360 370 380 390 400
LRLGLVQTQN EKELNGISAL EECLKLDPKN LEAMKTLAIS YINEGYDMSA
410 420 430 440 450
FTMLDKWAET KYPEIWSRIK QQDDKFQKEK GFTHIDMNAH ITKQFLQLAN
460 470 480 490 500
NLSTIDPEIQ LCLGLLFYTK DDFDKTIDCF ESALRVNPND ELMWNRLGAS
510 520 530 540 550
LANSNRSEEA IQAYHRALQL KPSFVRARYN LAVSSMNIGC FKEAAGYLLS
560 570 580 590 600
VLSMHEVNTN NKKGDVGSLL NTYNDTVIET LKRVFIAMNR DDLLQEVKPG
610
MDLKRFKGEF SF
Length:612
Mass (Da):69,324
Last modified:February 1, 1994 - v1
Checksum:i553251971E0BFD8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23076 Genomic DNA. Translation: AAA64794.1.
Z49701 Genomic DNA. Translation: CAA89730.1.
AY723785 Genomic DNA. Translation: AAU09702.1.
BK006938 Genomic DNA. Translation: DAA12084.1.
PIRiA49403.
RefSeqiNP_010530.1. NM_001180552.1.

Genome annotation databases

EnsemblFungiiYDR244W; YDR244W; YDR244W.
GeneIDi851831.
KEGGisce:YDR244W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23076 Genomic DNA. Translation: AAA64794.1.
Z49701 Genomic DNA. Translation: CAA89730.1.
AY723785 Genomic DNA. Translation: AAU09702.1.
BK006938 Genomic DNA. Translation: DAA12084.1.
PIRiA49403.
RefSeqiNP_010530.1. NM_001180552.1.

3D structure databases

ProteinModelPortaliP35056.
SMRiP35056.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32295. 78 interactors.
DIPiDIP-2475N.
IntActiP35056. 15 interactors.
MINTiMINT-433689.

Protein family/group databases

TCDBi3.A.20.1.5. the peroxisomal protein importer (ppi) family.

PTM databases

iPTMnetiP35056.

Proteomic databases

MaxQBiP35056.
PRIDEiP35056.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR244W; YDR244W; YDR244W.
GeneIDi851831.
KEGGisce:YDR244W.

Organism-specific databases

EuPathDBiFungiDB:YDR244W.
SGDiS000002652. PEX5.

Phylogenomic databases

GeneTreeiENSGT00390000013941.
HOGENOMiHOG000158146.
InParanoidiP35056.
KOiK13342.
OMAiERWISTK.
OrthoDBiEOG092C1NSE.

Enzyme and pathway databases

BioCyciYEAST:G3O-29817-MONOMER.

Miscellaneous databases

PROiP35056.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR024111. PTS1R_family.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10130. PTHR10130. 1 hit.
PfamiPF00515. TPR_1. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50005. TPR. 4 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEX5_YEAST
AccessioniPrimary (citable) accession number: P35056
Secondary accession number(s): D6VSM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2070 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.