Reviewed, UniProtKB/Swiss-Prot P35053 (GPC1_RAT)

Names and origin

Protein names

Recommended name:
    Glypican-1
Alternative name(s):
    HSPG M12
Cleaved into the following chain:
    1- Recommended name:
            Secreted glypican-1

Gene names

Name:

Gpc1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cell surface proteoglycan that bears heparan sulfate. May play an important role in the trophic and injury responses of neurons.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor; Extracellular side. Secreted glypican-1: Secreted › extracellular space By similarity.
Tissue specificity	Nervous system.
Post-translational modification	This cell-associated glypican is further processed to give rise to a medium-released species.
Sequence similarities	Belongs to the glypican family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Domain	Signal
PTM	GPI-anchor Glycoprotein Heparan sulfate Lipoprotein Proteoglycan
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell adhesion Ref.2 Non-traceable author statement. Source: RGD cell-cell signaling Non-traceable author statement. Source: RGD
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW proteinaceous extracellular matrix Inferred from electronic annotation. Source: InterPro
Molecular function	heparan sulfate proteoglycan binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.1
	Chain	24 – 530	507	Glypican-1		PRO_0000012299
	Chain	24 – ?		Secreted glypican-1		PRO_0000333839
	Propeptide	531 – 558	28	Removed in mature form Potential		PRO_0000012300
Amino acid modifications
	Lipidation	530	1	GPI-anchor amidated serine Potential
	Glycosylation	55	1	O-linked (Xyl...) (heparan sulfate) Potential
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	116	1	N-linked (GlcNAc...) Potential
	Glycosylation	486	1	O-linked (Xyl...) (heparan sulfate) Potential
	Glycosylation	488	1	O-linked (Xyl...) (heparan sulfate) Potential
	Glycosylation	490	1	O-linked (Xyl...) (heparan sulfate) Potential
Experimental info
	Sequence conflict	21	1	T → A in AAA41251. Ref.2
	Sequence conflict	312	1	Y → N in AAA41251. Ref.2
	Sequence conflict	362	1	A → G in AAA41251. Ref.2
	Sequence conflict	437	1	I → G AA sequence Ref.2
	Sequence conflict	443	1	E → D AA sequence Ref.2
	Sequence conflict	515	1	I → T in AAA41251. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P35053-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: E2878A854B9A1D7F	FASTA	558	61,734
	10 20 30 40 50 60 MELRARGWWL LCAAAALVAC TRGDPASKSR SCSEVRQIYG AKGFSLSDVP QAEISGEHLR 70 80 90 100 110 120 ICPQGYTCCT SEMEENLANH SRMELETALH DSSRALQATL ATQLHGIDDH FQRLLNDSER 130 140 150 160 170 180 TLQDAFPGAF GDLYTQNTRA FRDLYAELRL YYRGANLHLE ETLAEFWARL LERLFKQLHP 190 200 210 220 230 240 QLLLPDDYLD CLGKQAEALR PFGDAPRELR LRATRAFVAA RSFVQGLGVA SDVVRKVAQV 250 260 270 280 290 300 PLAPECSRAV MKLVYCAHCR GVPGARPCPD YCRNVLKGCL ANQADLDAEW RNLLDSMVLI 310 320 330 340 350 360 TDKFWGPSGA EYVIGSVHMW LAEAINALQD NKDTLTAKVI QGCGNPKVNP HGSGPEEKRR 370 380 390 400 410 420 RAKLALQEKS STGTLEKLVS EAKAQLRDIQ DYWISLPGTL CSEKMAMSPA SDDRCWNGIS 430 440 450 460 470 480 KGRYLPEVMG DGLANQINNP EVEVDITKPD MTIRQQIMQL KIMTNRLRGA YGGNDVDFQD 490 500 510 520 530 540 ASDDGSGSGS GGGCPDDACG RRVSKKSSSS RTPLIHALPG LSEQEGQKTS AATRPEPHYF 550 FLLFLFTLVL AAARPRWR

References

[1]	"Cloning of a major heparan sulfate proteoglycan from brain and identification as the rat form of glypican." Karthikeyan L., Maurel P., Rauch U., Margolis R.K., Margolis R.U. Biochem. Biophys. Res. Commun. 188:395-401(1992) [PubMed: 1417860] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-55 AND 424-445. Tissue: Brain.
[2]	"Neuronal expression of glypican, a cell-surface glycosylphosphatidylinositol-anchored heparan sulfate proteoglycan, in the adult rat nervous system." Litwack E.D., Stipp C.S., Kumbasar A., Lander A.D. J. Neurosci. 14:3713-3724(1994) [PubMed: 8207484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 83-112; 196-207 AND 422-443. Strain: New England Deaconess Hospital.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L02896 mRNA. Translation: AAA86439.1. L34067 mRNA. Translation: AAA41251.1.
IPI	IPI00194930.
PIR	I56545.
RefSeq	NP_110455.1.
UniGene	Rn.7044
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	58920.
KEGG	rno:58920.
Organism-specific databases
CTD	58920.
RGD	61853. Gpc1.
Phylogenomic databases
eggNOG	roNOG14876.
HOVERGEN	P35053.
Gene expression databases
Genevestigator	P35053.
Family and domain databases
InterPro	IPR001863. Glypican. IPR015502. Glypican-1. IPR019803. Glypican_CS. [Graphical view]
PANTHER	PTHR10822. Glypican. 1 hit. PTHR10822:SF8. Gpc1. 1 hit.
Pfam	PF01153. Glypican. 1 hit. [Graphical view]
PROSITE	PS01207. GLYPICAN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	611474.

Entry information

Entry name

GPC1_RAT

Accession

Primary (citable) accession number: P35053

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	February 9, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)