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Protein

Glypican-1

Gene

Gpc1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell surface proteoglycan that bears heparan sulfate. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. Binds Cu2+ or Zn2+ ions (By similarity). Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandCopper, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glypican-1
Alternative name(s):
HSPG M12
Cleaved into the following chain:
Gene namesi
Name:Gpc1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61853 Gpc1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000001229924 – 530Glypican-1Add BLAST507
ChainiPRO_000033383924 – ?Secreted glypican-1
PropeptideiPRO_0000012300531 – 558Removed in mature formSequence analysisAdd BLAST28

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi32 ↔ 68By similarity
Disulfide bondi62 ↔ 256By similarity
Disulfide bondi69 ↔ 259By similarity
Glycosylationi79N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi116N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi191 ↔ 343By similarity
Disulfide bondi246 ↔ 279By similarity
Disulfide bondi268 ↔ 415By similarity
Disulfide bondi272 ↔ 401By similarity
Glycosylationi486O-linked (Xyl...) (heparan sulfate) serineSequence analysis1
Glycosylationi488O-linked (Xyl...) (heparan sulfate) serineSequence analysis1
Glycosylationi490O-linked (Xyl...) (heparan sulfate) serineSequence analysis1
Lipidationi530GPI-anchor amidated serineSequence analysis1

Post-translational modificationi

S-nitrosylated in a Cu2+-dependent manner. Nitric acid (NO) is released from the nitrosylated cysteines by ascorbate or by some other reducing agent, in a Cu2+ or Zn2+ dependent manner. This free nitric oxide is then capable of cleaving the heparan sulfate side chains.
N- and O-glycosylated. N-glycosylation is mainly of the complex type containing sialic acid. O-glycosylated with heparan sulfate. The heparan sulfate chains can be cleaved either by the action of heparanase or, degraded by a deaminative process that uses nitric oxide (NO) released from the S-nitrosylated cysteines. This process is triggered by ascorbate, or by some other reducing agent, in a Cu2+- or Zn2+ dependent manner. Cu2+ ions are provided by ceruloproteins such as APP, PRNP or CP which associate with GCP1 in intracellular compartments or lipid rafts.1 Publication
This cell-associated glypican is further processed to give rise to a medium-released species.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Heparan sulfate, Lipoprotein, Proteoglycan

Proteomic databases

PaxDbiP35053
PRIDEiP35053

PTM databases

iPTMnetiP35053
UniCarbKBiP35053

Expressioni

Tissue specificityi

Nervous system.1 Publication

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248661, 1 interactor
IntActiP35053, 1 interactor
STRINGi10116.ENSRNOP00000066597

Structurei

3D structure databases

SMRiP35053
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glypican family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3821 Eukaryota
ENOG410XST2 LUCA
HOVERGENiHBG003464
InParanoidiP35053
KOiK08107
PhylomeDBiP35053

Family and domain databases

InterProiView protein in InterPro
IPR001863 Glypican
IPR015502 Glypican-1
IPR019803 Glypican_CS
PANTHERiPTHR10822 PTHR10822, 1 hit
PTHR10822:SF8 PTHR10822:SF8, 1 hit
PfamiView protein in Pfam
PF01153 Glypican, 1 hit
PROSITEiView protein in PROSITE
PS01207 GLYPICAN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35053-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELRARGWWL LCAAAALVAC TRGDPASKSR SCSEVRQIYG AKGFSLSDVP
60 70 80 90 100
QAEISGEHLR ICPQGYTCCT SEMEENLANH SRMELETALH DSSRALQATL
110 120 130 140 150
ATQLHGIDDH FQRLLNDSER TLQDAFPGAF GDLYTQNTRA FRDLYAELRL
160 170 180 190 200
YYRGANLHLE ETLAEFWARL LERLFKQLHP QLLLPDDYLD CLGKQAEALR
210 220 230 240 250
PFGDAPRELR LRATRAFVAA RSFVQGLGVA SDVVRKVAQV PLAPECSRAV
260 270 280 290 300
MKLVYCAHCR GVPGARPCPD YCRNVLKGCL ANQADLDAEW RNLLDSMVLI
310 320 330 340 350
TDKFWGPSGA EYVIGSVHMW LAEAINALQD NKDTLTAKVI QGCGNPKVNP
360 370 380 390 400
HGSGPEEKRR RAKLALQEKS STGTLEKLVS EAKAQLRDIQ DYWISLPGTL
410 420 430 440 450
CSEKMAMSPA SDDRCWNGIS KGRYLPEVMG DGLANQINNP EVEVDITKPD
460 470 480 490 500
MTIRQQIMQL KIMTNRLRGA YGGNDVDFQD ASDDGSGSGS GGGCPDDACG
510 520 530 540 550
RRVSKKSSSS RTPLIHALPG LSEQEGQKTS AATRPEPHYF FLLFLFTLVL

AAARPRWR
Length:558
Mass (Da):61,734
Last modified:February 1, 1994 - v1
Checksum:iE2878A854B9A1D7F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21T → A in AAA41251 (PubMed:8207484).Curated1
Sequence conflicti312Y → N in AAA41251 (PubMed:8207484).Curated1
Sequence conflicti362A → G in AAA41251 (PubMed:8207484).Curated1
Sequence conflicti437I → G AA sequence (PubMed:8207484).Curated1
Sequence conflicti443E → D AA sequence (PubMed:8207484).Curated1
Sequence conflicti515I → T in AAA41251 (PubMed:8207484).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02896 mRNA Translation: AAA86439.1
L34067 mRNA Translation: AAA41251.1
PIRiI56545
RefSeqiNP_110455.1, NM_030828.1
UniGeneiRn.7044

Genome annotation databases

GeneIDi58920
KEGGirno:58920

Similar proteinsi

Entry informationi

Entry nameiGPC1_RAT
AccessioniPrimary (citable) accession number: P35053
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health