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P35048

- TRYP_SIMVI

UniProt

P35048 - TRYP_SIMVI

Protein

Trypsin

Gene
N/A
Organism
Simulium vittatum (Striped black fly)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    • Comment

    Functioni

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei76 – 761Charge relay systemBy similarity
    Active sitei120 – 1201Charge relay systemBy similarity
    Sitei210 – 2101Required for specificityBy similarity
    Active sitei216 – 2161Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin (EC:3.4.21.4)
    OrganismiSimulium vittatum (Striped black fly)
    Taxonomic identifieri7192 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraChironomoideaSimuliidaeSimulium

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei‹1 – 21›21Sequence AnalysisAdd
    BLAST
    Propeptidei22 – 309Activation peptidePRO_0000028299
    Chaini31 – 247217TrypsinPRO_0000028300Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi61 ↔ 77PROSITE-ProRule annotation
    Disulfide bondi185 ↔ 201PROSITE-ProRule annotation
    Disulfide bondi212 ↔ 236PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Expressioni

    Tissue specificityi

    Midgut.

    Structurei

    3D structure databases

    ProteinModelPortaliP35048.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – ›247›217Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35048-1 [UniParc]FASTAAdd to Basket

    « Hide

    LTTVISYFAL VAFALVGVSY ATPKASINGR IVGGEMTDIS LIPYQVSVQT    50
    AISSYGFIHH CGGSIISPRW VVTAAHCAQK TNSAYQVYTG SSNKVEGGQA 100
    YRVKTIINHP LYDEETTDYD VALLELAEPI VMNYKTAAIE LAEVGEEVET 150
    DAMAIVSGWG DTKNFGEEPN MLRSAEVPIF DQELCAYLNA NHGVVTERMI 200
    CAGYLAGGRD SCQGDSGGPL AVDGKLVGIV SWGVGCAQSN FPGVYGI 247
    Length:247
    Mass (Da):26,247
    Last modified:February 1, 1994 - v1
    Checksum:i90891564E3BA5FCD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Non-terminal residuei247 – 2471

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08428 mRNA. Translation: AAA18530.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08428 mRNA. Translation: AAA18530.2 .

    3D structure databases

    ProteinModelPortali P35048.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gut-specific genes from the black fly Simulium vittatum encoding trypsin-like and carboxypeptidase-like proteins."
      Ramos A., Mahowald A., Jacobs-Lorena M.
      Insect Mol. Biol. 1:149-163(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Gut.

    Entry informationi

    Entry nameiTRYP_SIMVI
    AccessioniPrimary (citable) accession number: P35048
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3