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P35048 (TRYP_SIMVI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trypsin

EC=3.4.21.4
OrganismSimulium vittatum (Striped black fly)
Taxonomic identifier7192 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraChironomoideaSimuliidaeSimulium

Protein attributes

Sequence length247 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Subcellular location

Secretedextracellular space.

Tissue specificity

Midgut.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Zymogen
Gene Ontology (GO)
   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide‹1 – 21›21 Potential
Propeptide22 – 309Activation peptide
PRO_0000028299
Chain31 – 247217Trypsin
PRO_0000028300

Regions

Domain31 – ›247›217Peptidase S1

Sites

Active site761Charge relay system By similarity
Active site1201Charge relay system By similarity
Active site2161Charge relay system By similarity
Site2101Required for specificity By similarity

Amino acid modifications

Disulfide bond61 ↔ 77 By similarity
Disulfide bond185 ↔ 201 By similarity
Disulfide bond212 ↔ 236 By similarity

Experimental info

Non-terminal residue11
Non-terminal residue2471

Sequences

Sequence LengthMass (Da)Tools
P35048 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 90891564E3BA5FCD

FASTA24726,247
        10         20         30         40         50         60 
LTTVISYFAL VAFALVGVSY ATPKASINGR IVGGEMTDIS LIPYQVSVQT AISSYGFIHH 

        70         80         90        100        110        120 
CGGSIISPRW VVTAAHCAQK TNSAYQVYTG SSNKVEGGQA YRVKTIINHP LYDEETTDYD 

       130        140        150        160        170        180 
VALLELAEPI VMNYKTAAIE LAEVGEEVET DAMAIVSGWG DTKNFGEEPN MLRSAEVPIF 

       190        200        210        220        230        240 
DQELCAYLNA NHGVVTERMI CAGYLAGGRD SCQGDSGGPL AVDGKLVGIV SWGVGCAQSN 


FPGVYGI 

« Hide

References

[1]"Gut-specific genes from the black fly Simulium vittatum encoding trypsin-like and carboxypeptidase-like proteins."
Ramos A., Mahowald A., Jacobs-Lorena M.
Insect Mol. Biol. 1:149-163(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Gut.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L08428 mRNA. Translation: AAA18530.2.

3D structure databases

ProteinModelPortalP35048.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRYP_SIMVI
AccessionPrimary (citable) accession number: P35048
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 14, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries