P35048 (TRYP_SIMVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Trypsin EC=3.4.21.4
Organism	Simulium vittatum (Striped black fly)
Taxonomic identifier	7192 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicimorpha › Chironomoidea › Simuliidae › Simuliinae › Simuliini › Simulium › Psilozia

Protein attributes

Sequence length	247 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted › extracellular space.
Tissue specificity	Midgut.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	‹1 – 21	›21	Potential
	Propeptide	22 – 30	9	Activation peptide		PRO_0000028299
	Chain	31 – 247	217	Trypsin		PRO_0000028300
Regions
	Domain	31 – ›247	›217	Peptidase S1
Sites
	Active site	76	1	Charge relay system By similarity
	Active site	120	1	Charge relay system By similarity
	Active site	216	1	Charge relay system By similarity
	Site	210	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	61 ↔ 77		By similarity
	Disulfide bond	185 ↔ 201		By similarity
	Disulfide bond	212 ↔ 236		By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	247	1

Sequences

	Sequence		Length	Mass (Da)
	P35048 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 90891564E3BA5FCD	FASTA	247	26,247
	10 20 30 40 50 60 LTTVISYFAL VAFALVGVSY ATPKASINGR IVGGEMTDIS LIPYQVSVQT AISSYGFIHH 70 80 90 100 110 120 CGGSIISPRW VVTAAHCAQK TNSAYQVYTG SSNKVEGGQA YRVKTIINHP LYDEETTDYD 130 140 150 160 170 180 VALLELAEPI VMNYKTAAIE LAEVGEEVET DAMAIVSGWG DTKNFGEEPN MLRSAEVPIF 190 200 210 220 230 240 DQELCAYLNA NHGVVTERMI CAGYLAGGRD SCQGDSGGPL AVDGKLVGIV SWGVGCAQSN FPGVYGI

References

[1]	"Gut-specific genes from the black fly Simulium vittatum encoding trypsin-like and carboxypeptidase-like proteins." Ramos A., Mahowald A., Jacobs-Lorena M. Insect Mol. Biol. 1:149-163(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Gut.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L08428 mRNA. Translation: AAA18530.2.
3D structure databases
ProteinModelPortal	P35048.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYP_SIMVI

Accession

Primary (citable) accession number: P35048

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)