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Protein

Trypsin

Gene
N/A
Organism
Simulium vittatum (Striped black fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei76 – 761Charge relay systemBy similarity
Active sitei120 – 1201Charge relay systemBy similarity
Sitei210 – 2101Required for specificityBy similarity
Active sitei216 – 2161Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin (EC:3.4.21.4)
OrganismiSimulium vittatum (Striped black fly)
Taxonomic identifieri7192 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraChironomoideaSimuliidaeSimulium

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 21›21Sequence AnalysisAdd
BLAST
Propeptidei22 – 309Activation peptidePRO_0000028299
Chaini31 – 247217TrypsinPRO_0000028300Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi61 ↔ 77PROSITE-ProRule annotation
Disulfide bondi185 ↔ 201PROSITE-ProRule annotation
Disulfide bondi212 ↔ 236PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Tissue specificityi

Midgut.

Structurei

3D structure databases

ProteinModelPortaliP35048.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – ›247›217Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35048-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
LTTVISYFAL VAFALVGVSY ATPKASINGR IVGGEMTDIS LIPYQVSVQT
60 70 80 90 100
AISSYGFIHH CGGSIISPRW VVTAAHCAQK TNSAYQVYTG SSNKVEGGQA
110 120 130 140 150
YRVKTIINHP LYDEETTDYD VALLELAEPI VMNYKTAAIE LAEVGEEVET
160 170 180 190 200
DAMAIVSGWG DTKNFGEEPN MLRSAEVPIF DQELCAYLNA NHGVVTERMI
210 220 230 240
CAGYLAGGRD SCQGDSGGPL AVDGKLVGIV SWGVGCAQSN FPGVYGI
Length:247
Mass (Da):26,247
Last modified:February 1, 1994 - v1
Checksum:i90891564E3BA5FCD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei247 – 2471

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08428 mRNA. Translation: AAA18530.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08428 mRNA. Translation: AAA18530.2.

3D structure databases

ProteinModelPortaliP35048.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Gut-specific genes from the black fly Simulium vittatum encoding trypsin-like and carboxypeptidase-like proteins."
    Ramos A., Mahowald A., Jacobs-Lorena M.
    Insect Mol. Biol. 1:149-163(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Gut.

Entry informationi

Entry nameiTRYP_SIMVI
AccessioniPrimary (citable) accession number: P35048
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: January 7, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.