Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P35047 (TRYC_MANSE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length256 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Subcellular location

Secretedextracellular space.

Tissue specificity

Midgut.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Sequence caution

The sequence AAA29341.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Zymogen
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Propeptide18 – 247Activation peptide
PRO_0000028295
Chain25 – 256232Trypsin, alkaline C
PRO_0000028296

Regions

Domain25 – 256232Peptidase S1

Sites

Active site701Charge relay system By similarity
Active site1151Charge relay system By similarity
Active site2131Charge relay system By similarity
Site2071Required for specificity By similarity

Amino acid modifications

Disulfide bond55 ↔ 71 By similarity
Disulfide bond180 ↔ 197 By similarity
Disulfide bond209 ↔ 233 By similarity

Sequences

Sequence LengthMass (Da)Tools
P35047 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: EE6C1D79F92F0880

FASTA25627,474
        10         20         30         40         50         60 
MRLFLALLAL GFAAVAAVPA NPQRIVGGST TTIQQYPTIV ALLFSRNGNT FFQACGGTIL 

        70         80         90        100        110        120 
NNRNVLTAAH CPHGDAVNRW RVRSGSTFAN SGGAVHNLNS VRIHPNYNRR NLDNDIAIMR 

       130        140        150        160        170        180 
TASNIAFNNA AQPARIAGAN YNLGDNQVVW AAGWGAIRSG GPSSEQLRHV QVWTVNQATC 

       190        200        210        220        230        240 
RSRYASIGRT VTDNMLCSGW LDVGGRDQCQ GDSGGPLYHN GVVVGVCSWG EECALARFPG 

       250 
VNARVTRYTS WISNNS 

« Hide

References

[1]"Sequence of three cDNAs encoding an alkaline midgut trypsin from Manduca sexta."
Peterson A.M., Barillas-Mury C.V., Wells M.A.
Insect Biochem. Mol. Biol. 24:463-471(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Midgut.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L16807 mRNA. Translation: AAA29341.1. Different initiation.

3D structure databases

ProteinModelPortalP35047.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.420.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRYC_MANSE
AccessionPrimary (citable) accession number: P35047
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 16, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries