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P35046

- TRYB_MANSE

UniProt

P35046 - TRYB_MANSE

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Protein

Trypsin, alkaline B

Gene
N/A
Organism
Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei70 – 701Charge relay system By similarity
Active sitei115 – 1151Charge relay system By similarity
Sitei207 – 2071Required for specificity By similarity
Active sitei213 – 2131Charge relay system By similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.420.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin, alkaline B (EC:3.4.21.4)
OrganismiManduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Taxonomic identifieri7130 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaSphingidaeSphinginaeSphinginiManduca

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717 Reviewed predictionAdd
BLAST
Propeptidei18 – 247Activation peptidePRO_0000028293
Chaini25 – 256232Trypsin, alkaline BPRO_0000028294Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi55 ↔ 71 By similarity
Disulfide bondi180 ↔ 197 By similarity
Disulfide bondi209 ↔ 233 By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Tissue specificityi

Midgut.

Structurei

3D structure databases

ProteinModelPortaliP35046.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 256232Peptidase S1Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35046-1 [UniParc]FASTAAdd to Basket

« Hide

MRLFLALLAL GFAAVAAVPA NPQRIVGGST TTIQQYPTIV ALLFSRNGNT    50
FFQACGGIIL NNRNILTAAH CPHGDAVNRW RVRSGSTFAN SGGAVHNLNR 100
VRIHPNYNSR TLDNDIAIMR TSSNIAFNNA AQPARIAGAN YNVGDNQVVW 150
AAGWGDIRSG GPPSEQLRHV QVWTVNQATC RSRYASIGRS VTDNMLCSGW 200
LDVGGRDQCQ GDSGGPLYHN GVVVGVSSWG EECALARFPG VNARVSRFAN 250
WIRNNS 256
Length:256
Mass (Da):27,549
Last modified:February 1, 1994 - v1
Checksum:i2108BE041DB9CAE0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L16806 mRNA. Translation: AAA29340.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L16806 mRNA. Translation: AAA29340.1 .

3D structure databases

ProteinModelPortali P35046.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S01.420.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of three cDNAs encoding an alkaline midgut trypsin from Manduca sexta."
    Peterson A.M., Barillas-Mury C.V., Wells M.A.
    Insect Biochem. Mol. Biol. 24:463-471(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Midgut.

Entry informationi

Entry nameiTRYB_MANSE
AccessioniPrimary (citable) accession number: P35046
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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