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P35046 (TRYB_MANSE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length256 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Subcellular location

Secretedextracellular space.

Tissue specificity

Midgut.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Zymogen
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Propeptide18 – 247Activation peptide
PRO_0000028293
Chain25 – 256232Trypsin, alkaline B
PRO_0000028294

Regions

Domain25 – 256232Peptidase S1

Sites

Active site701Charge relay system By similarity
Active site1151Charge relay system By similarity
Active site2131Charge relay system By similarity
Site2071Required for specificity By similarity

Amino acid modifications

Disulfide bond55 ↔ 71 By similarity
Disulfide bond180 ↔ 197 By similarity
Disulfide bond209 ↔ 233 By similarity

Sequences

Sequence LengthMass (Da)Tools
P35046 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 2108BE041DB9CAE0

FASTA25627,549
        10         20         30         40         50         60 
MRLFLALLAL GFAAVAAVPA NPQRIVGGST TTIQQYPTIV ALLFSRNGNT FFQACGGIIL 

        70         80         90        100        110        120 
NNRNILTAAH CPHGDAVNRW RVRSGSTFAN SGGAVHNLNR VRIHPNYNSR TLDNDIAIMR 

       130        140        150        160        170        180 
TSSNIAFNNA AQPARIAGAN YNVGDNQVVW AAGWGDIRSG GPPSEQLRHV QVWTVNQATC 

       190        200        210        220        230        240 
RSRYASIGRS VTDNMLCSGW LDVGGRDQCQ GDSGGPLYHN GVVVGVSSWG EECALARFPG 

       250 
VNARVSRFAN WIRNNS 

« Hide

References

[1]"Sequence of three cDNAs encoding an alkaline midgut trypsin from Manduca sexta."
Peterson A.M., Barillas-Mury C.V., Wells M.A.
Insect Biochem. Mol. Biol. 24:463-471(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Midgut.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L16806 mRNA. Translation: AAA29340.1.

3D structure databases

ProteinModelPortalP35046.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.420.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRYB_MANSE
AccessionPrimary (citable) accession number: P35046
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 16, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries