P35046 (TRYB_MANSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Trypsin, alkaline B EC=3.4.21.4
Organism	Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Taxonomic identifier	7130 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Amphiesmenoptera › Lepidoptera › Glossata › Neolepidoptera › Heteroneura › Ditrysia › Obtectomera › Bombycoidea › Sphingidae › Sphinginae › Sphingini › Manduca

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted › extracellular space.
Tissue specificity	Midgut.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 24	7	Activation peptide		PRO_0000028293
	Chain	25 – 256	232	Trypsin, alkaline B		PRO_0000028294
Regions
	Domain	25 – 256	232	Peptidase S1
Sites
	Active site	70	1	Charge relay system By similarity
	Active site	115	1	Charge relay system By similarity
	Active site	213	1	Charge relay system By similarity
	Site	207	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	55 ↔ 71		By similarity
	Disulfide bond	180 ↔ 197		By similarity
	Disulfide bond	209 ↔ 233		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35046 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 2108BE041DB9CAE0	FASTA	256	27,549
	10 20 30 40 50 60 MRLFLALLAL GFAAVAAVPA NPQRIVGGST TTIQQYPTIV ALLFSRNGNT FFQACGGIIL 70 80 90 100 110 120 NNRNILTAAH CPHGDAVNRW RVRSGSTFAN SGGAVHNLNR VRIHPNYNSR TLDNDIAIMR 130 140 150 160 170 180 TSSNIAFNNA AQPARIAGAN YNVGDNQVVW AAGWGDIRSG GPPSEQLRHV QVWTVNQATC 190 200 210 220 230 240 RSRYASIGRS VTDNMLCSGW LDVGGRDQCQ GDSGGPLYHN GVVVGVSSWG EECALARFPG 250 VNARVSRFAN WIRNNS

References

[1]	"Sequence of three cDNAs encoding an alkaline midgut trypsin from Manduca sexta." Peterson A.M., Barillas-Mury C.V., Wells M.A. Insect Biochem. Mol. Biol. 24:463-471(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Midgut.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L16806 mRNA. Translation: AAA29340.1.
3D structure databases
ProteinModelPortal	P35046.
ModBase	Search...
Protein family/group databases
MEROPS	S01.420.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYB_MANSE

Accession

Primary (citable) accession number: P35046

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)