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P35045

- TRYA_MANSE

UniProt

P35045 - TRYA_MANSE

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Protein
Trypsin, alkaline A
Gene
N/A
Organism
Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei70 – 701Charge relay system By similarity
Active sitei115 – 1151Charge relay system By similarity
Sitei207 – 2071Required for specificity By similarity
Active sitei213 – 2131Charge relay system By similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.420.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin, alkaline A (EC:3.4.21.4)
    OrganismiManduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
    Taxonomic identifieri7130 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaSphingidaeSphinginaeSphinginiManduca

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717 Reviewed prediction
    Add
    BLAST
    Propeptidei18 – 247Activation peptide
    PRO_0000028291
    Chaini25 – 256232Trypsin, alkaline A
    PRO_0000028292Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi55 ↔ 71 By similarity
    Disulfide bondi180 ↔ 197 By similarity
    Disulfide bondi209 ↔ 233 By similarity

    Keywords - PTMi

    Disulfide bond, Zymogen

    Expressioni

    Tissue specificityi

    Midgut.

    Structurei

    3D structure databases

    ProteinModelPortaliP35045.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 256232Peptidase S1
    Add
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35045-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLFLALLAL GFAAVAAVPA YPQRIVGGST TTIQQYPTIV ALLFSRNGNT    50
    FFQACGGIIL NNRNVLTAAH CPHGDAVNRW RVRSGSTYAN SGGAVHNLNR 100
    VRIHPNFNRR TLDNDIAIMR TTSNIAFNNA AQPARIAGAN YNLGDNQVVW 150
    AAGWGAIRSG GPSSEQLRHV QVWTVNQATC RSRYASIGRS VTDNMLCSGW 200
    LDVGGRDQCQ GDSGGPLYHN GVVVGVCSWG EECALARFPG VNARVSRFAN 250
    WIRNNS 256
    Length:256
    Mass (Da):27,643
    Last modified:February 1, 1994 - v1
    Checksum:i8E9FEBB979790814
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L16805 mRNA. Translation: AAA29339.1.
    PIRiT10109.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L16805 mRNA. Translation: AAA29339.1 .
    PIRi T10109.

    3D structure databases

    ProteinModelPortali P35045.
    ModBasei Search...

    Protein family/group databases

    MEROPSi S01.420.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of three cDNAs encoding an alkaline midgut trypsin from Manduca sexta."
      Peterson A.M., Barillas-Mury C.V., Wells M.A.
      Insect Biochem. Mol. Biol. 24:463-471(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Midgut.

    Entry informationi

    Entry nameiTRYA_MANSE
    AccessioniPrimary (citable) accession number: P35045
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: May 14, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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