Reviewed, UniProtKB/Swiss-Prot P35044 (TRYA4_LUCCU)

Names and origin

Protein names	Recommended name:     Trypsin alpha-4     EC=3.4.21.4
Organism	Lucilia cuprina (Green bottle fly) (Australian sheep blowfly)
Taxonomic identifier	7375 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Oestroidea › Calliphoridae › Luciliinae › Lucilia

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 30	14	Activation peptide		PRO_0000028289
	Chain	31 – 255	225	Trypsin alpha-4		PRO_0000028290
Regions
	Domain	31 – 253	223	Peptidase S1
Sites
	Active site	71	1	Charge relay system By similarity
	Active site	116	1	Charge relay system By similarity
	Active site	209	1	Charge relay system By similarity
	Site	203	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	56 ↔ 72		By similarity
	Disulfide bond	179 ↔ 196		By similarity
	Disulfide bond	205 ↔ 229		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35044-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 5756AEDA78A3ED46	FASTA	255	26,119
	10 20 30 40 50 60 MLKFVVLLCA ISCALGAAVP EGMVPQLDGR IVGGVATTIS SFPWQISLQR SGSHSCGGSV 70 80 90 100 110 120 YNSRIIVTAA HCLQSVSTSV LKVRRGSSYW NSGGVVVSVA AFKNHEGYNP KTMVNDIAVI 130 140 150 160 170 180 RLSSSLTMSS TIKAIALTTA APANGAAATV SGWGTTSSGG SIPAQLRYVD LKIVGRTQCA 190 200 210 220 230 240 SSTYGYGSQI KPSMICAYTV GKDSCQGDSG GPLVSGGRLV GVVSWGYGCA FANYPGVYAD 250 VAALRTWVVS AASSV

References

[1]	"Isolation of a trypsin-like serine protease gene family from the sheep blowfly Lucilia cuprina." Casu R.E., Jarmey J.M., Elvin C.M., Eisemann C.H. Insect Mol. Biol. 3:159-170(1994) [PubMed: 7894748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L15632 Genomic DNA. Translation: AAA65932.1.
3D structure databases
HSSP	HSSP built from PDB template 1FDP based on UniProtKB P00746.
ModBase	Search...
Protein family/group databases
MEROPS	S01.110.
Enzyme and pathway databases
BRENDA	3.4.21.4. 119350.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYA4_LUCCU

Accession

Primary (citable) accession number: P35044

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)