P35043 (TRYA3_LUCCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Trypsin alpha-3 EC=3.4.21.4
Organism	Lucilia cuprina (Green bottle fly) (Australian sheep blowfly)
Taxonomic identifier	7375 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Calyptratae › Oestroidea › Calliphoridae › Luciliinae › Lucilia

Protein attributes

Sequence length	165 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 165	›165	Trypsin alpha-3		PRO_0000088717
Regions
	Domain	‹1 – 163	›163	Peptidase S1
Sites
	Active site	26	1	Charge relay system By similarity
	Active site	119	1	Charge relay system By similarity
	Site	113	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	89 ↔ 106		By similarity
	Disulfide bond	115 ↔ 139		By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P35043 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 26160B1AFF80F1CD	FASTA	165	16,570
	10 20 30 40 50 60 NSGGVLVSVA AFKNHEGYNS KTMVNDIAVI RLSSSLTTSS TIKTIGLATA APANGAAATV 70 80 90 100 110 120 SGWGTTSSGG SIPSQLRYVD VKIVGRTQCA SSTYGYGSEI KASMICAYTV GKDSCQGDSG 130 140 150 160 GPLVSGGRLV GVVSWGYGCA AVNYPGVYAD VAALRSWVVS AANSV

References

[1]	"Isolation of a trypsin-like serine protease gene family from the sheep blowfly Lucilia cuprina." Casu R.E., Jarmey J.M., Elvin C.M., Eisemann C.H. Insect Mol. Biol. 3:159-170(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	L15632 Genomic DNA. Translation: AAA65931.1.
3D structure databases
ProteinModelPortal	P35043.
ModBase	Search...
Protein family/group databases
MEROPS	S01.112.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. Partial match. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYA3_LUCCU

Accession

Primary (citable) accession number: P35043

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)