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P35042

- TRYP_CHOFU

UniProt

P35042 - TRYP_CHOFU

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Protein

Trypsin CFT-1

Gene
N/A
Organism
Choristoneura fumiferana (Spruce budworm moth) (Archips fumiferana)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Responsible for the activation of delta-endotoxin from Bacillus thuringiensis.

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei70 – 701Charge relay system By similarity
Active sitei115 – 1151Charge relay system By similarity
Sitei207 – 2071Required for specificity By similarity
Active sitei213 – 2131Charge relay system By similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.112.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin CFT-1 (EC:3.4.21.4)
OrganismiChoristoneura fumiferana (Spruce budworm moth) (Archips fumiferana)
Taxonomic identifieri7141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaTortricoideaTortricidaeTortricinaeChoristoneura

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717 Reviewed predictionAdd
BLAST
Propeptidei18 – 247Activation peptidePRO_0000028257
Chaini25 – 256232Trypsin CFT-1PRO_0000028258Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi55 ↔ 71 By similarity
Disulfide bondi180 ↔ 197 By similarity
Disulfide bondi209 ↔ 233 By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP35042.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 256232Peptidase S1Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35042-1 [UniParc]FASTAAdd to Basket

« Hide

MRVTLALVAL CLASVAALPE KQQRIVGGSV TTIEQWPSGS ALLYSWNLVT    50
YSQACGGAIL NTRSILSAAH CFIGDAANRW RIRTGSTWAN SGGVVHNTAL 100
IIIHPSYNTR TLDNDIAILR SATTIAQNNQ ARPASIAGAN YNLADNQAVW 150
AIGWGATCPG CAGSEQLRHI QIWTVNQNTC RSRYLEVGGT ITDNMLCSGW 200
LDVGGRDQCQ GDSGGPLFHN NVVVGVCSWG QSCALARYPG VNARVSRFTA 250
WIQANA 256
Length:256
Mass (Da):27,333
Last modified:February 1, 1994 - v1
Checksum:i4C2A8CBA74CFEF67
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L04749 mRNA. Translation: AAA81525.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L04749 mRNA. Translation: AAA81525.1 .

3D structure databases

ProteinModelPortali P35042.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S01.112.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and characterization of a full-length trypsin-encoding cDNA clone from the Lepidopteran insect, Choristoneura fumiferana."
    Wang S., Magoulas C., Hickey D.A.
    Gene 136:375-376(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Purification and characterization of a trypsin-like digestive enzyme from spruce budworm (Choristoneura fumiferana) responsible for the activation of delta-endotoxin from Bacillus thuringiensis."
    Milne R., Kaplan H.
    Insect Biochem. Mol. Biol. 23:663-673(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-69, CHARACTERIZATION.
    Tissue: Gut juice.

Entry informationi

Entry nameiTRYP_CHOFU
AccessioniPrimary (citable) accession number: P35042
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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