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P35042

- TRYP_CHOFU

UniProt

P35042 - TRYP_CHOFU

Protein

Trypsin CFT-1

Gene
N/A
Organism
Choristoneura fumiferana (Spruce budworm moth) (Archips fumiferana)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    • Comment

    Functioni

    Responsible for the activation of delta-endotoxin from Bacillus thuringiensis.

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei70 – 701Charge relay systemBy similarity
    Active sitei115 – 1151Charge relay systemBy similarity
    Sitei207 – 2071Required for specificityBy similarity
    Active sitei213 – 2131Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.112.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin CFT-1 (EC:3.4.21.4)
    OrganismiChoristoneura fumiferana (Spruce budworm moth) (Archips fumiferana)
    Taxonomic identifieri7141 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaTortricoideaTortricidaeTortricinaeChoristoneura

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Propeptidei18 – 247Activation peptide1 PublicationPRO_0000028257
    Chaini25 – 256232Trypsin CFT-1PRO_0000028258Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi55 ↔ 71PROSITE-ProRule annotation
    Disulfide bondi180 ↔ 197PROSITE-ProRule annotation
    Disulfide bondi209 ↔ 233PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Structurei

    3D structure databases

    ProteinModelPortaliP35042.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 256232Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35042-1 [UniParc]FASTAAdd to Basket

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    MRVTLALVAL CLASVAALPE KQQRIVGGSV TTIEQWPSGS ALLYSWNLVT    50
    YSQACGGAIL NTRSILSAAH CFIGDAANRW RIRTGSTWAN SGGVVHNTAL 100
    IIIHPSYNTR TLDNDIAILR SATTIAQNNQ ARPASIAGAN YNLADNQAVW 150
    AIGWGATCPG CAGSEQLRHI QIWTVNQNTC RSRYLEVGGT ITDNMLCSGW 200
    LDVGGRDQCQ GDSGGPLFHN NVVVGVCSWG QSCALARYPG VNARVSRFTA 250
    WIQANA 256
    Length:256
    Mass (Da):27,333
    Last modified:February 1, 1994 - v1
    Checksum:i4C2A8CBA74CFEF67
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L04749 mRNA. Translation: AAA81525.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L04749 mRNA. Translation: AAA81525.1 .

    3D structure databases

    ProteinModelPortali P35042.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.112.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a full-length trypsin-encoding cDNA clone from the Lepidopteran insect, Choristoneura fumiferana."
      Wang S., Magoulas C., Hickey D.A.
      Gene 136:375-376(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Purification and characterization of a trypsin-like digestive enzyme from spruce budworm (Choristoneura fumiferana) responsible for the activation of delta-endotoxin from Bacillus thuringiensis."
      Milne R., Kaplan H.
      Insect Biochem. Mol. Biol. 23:663-673(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-69, CHARACTERIZATION.
      Tissue: Gut juice.

    Entry informationi

    Entry nameiTRYP_CHOFU
    AccessioniPrimary (citable) accession number: P35042
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3