Reviewed, UniProtKB/Swiss-Prot P35042 (TRYP_CHOFU)

Names and origin

Protein names	Recommended name:     Trypsin CFT-1     EC=3.4.21.4
Organism	Choristoneura fumiferana (Spruce budworm)
Taxonomic identifier	7141 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Lepidoptera › Glossata › Ditrysia › Tortricoidea › Tortricidae › Tortricinae › Choristoneura

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for the activation of delta-endotoxin from Bacillus thuringiensis.
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 24	7	Activation peptide		PRO_0000028257
	Chain	25 – 256	232	Trypsin CFT-1		PRO_0000028258
Regions
	Domain	25 – 256	232	Peptidase S1
Sites
	Active site	70	1	Charge relay system By similarity
	Active site	115	1	Charge relay system By similarity
	Active site	213	1	Charge relay system By similarity
	Site	207	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	55 ↔ 71		By similarity
	Disulfide bond	180 ↔ 197		By similarity
	Disulfide bond	209 ↔ 233		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35042-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 4C2A8CBA74CFEF67	FASTA	256	27,333
	10 20 30 40 50 60 MRVTLALVAL CLASVAALPE KQQRIVGGSV TTIEQWPSGS ALLYSWNLVT YSQACGGAIL 70 80 90 100 110 120 NTRSILSAAH CFIGDAANRW RIRTGSTWAN SGGVVHNTAL IIIHPSYNTR TLDNDIAILR 130 140 150 160 170 180 SATTIAQNNQ ARPASIAGAN YNLADNQAVW AIGWGATCPG CAGSEQLRHI QIWTVNQNTC 190 200 210 220 230 240 RSRYLEVGGT ITDNMLCSGW LDVGGRDQCQ GDSGGPLFHN NVVVGVCSWG QSCALARYPG 250 VNARVSRFTA WIQANA

References

[1]	"Isolation and characterization of a full-length trypsin-encoding cDNA clone from the Lepidopteran insect, Choristoneura fumiferana." Wang S., Magoulas C., Hickey D.A. Gene 136:375-376(1993) [PubMed: 8294037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Purification and characterization of a trypsin-like digestive enzyme from spruce budworm (Choristoneura fumiferana) responsible for the activation of delta-endotoxin from Bacillus thuringiensis." Milne R., Kaplan H. Insect Biochem. Mol. Biol. 23:663-673(1993) [PubMed: 8353523] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-69, CHARACTERIZATION. Tissue: Gut juice.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L04749 mRNA. Translation: AAA81525.1.
3D structure databases
SMR	P35042. Positions 21-255.
ModBase	Search...
Protein family/group databases
MEROPS	S01.112.
Enzyme and pathway databases
BRENDA	3.4.21.4. 255525.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR009003. Ser/Cys_Pept_Trypsin-like. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYP_CHOFU

Accession

Primary (citable) accession number: P35042

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 19, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)