Reviewed, UniProtKB/Swiss-Prot P35041 (TRY7_ANOGA)

Names and origin

Protein names

Recommended name:
    Trypsin-7
    EC=3.4.21.4

Gene names

Name:	TRYP7
ORF Names:	AGAP008293

Organism

Anopheles gambiae (African malaria mosquito) [Complete proteome]

Taxonomic identifier

7165 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Anophelinae › Anopheles

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Constitutive trypsin that is expressed 2 days after emergence, coinciding with host seeking behavior of the female. Ref.1
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted. Ref.1
Tissue specificity	Expressed in the midgut. Expression levels drop a few hours after blood feeding and pick up again 28 hours later. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 41	23	Activation peptide		PRO_0000028255
	Chain	42 – 267	226	Trypsin-7		PRO_0000028256
Regions
	Domain	42 – 266	225	Peptidase S1
Sites
	Active site	82	1	Charge relay system By similarity
	Active site	127	1	Charge relay system By similarity
	Active site	222	1	Charge relay system By similarity
	Site	216	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	67 ↔ 83		By similarity
	Disulfide bond	192 ↔ 207		By similarity
	Disulfide bond	218 ↔ 242		By similarity
Experimental info
	Sequence conflict	118 – 124	7	KYNEYNT → NYDDSTI in CAA80516. Ref.1
	Sequence conflict	170 – 173	4	KSAT → HNAA in CAA80516. Ref.1
	Sequence conflict	199	1	D → E in CAA80516. Ref.1
	Sequence conflict	240	1	S → A in CAA80516. Ref.1
	Sequence conflict	256	1	V → I in CAA80516. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P35041-1 [UniParc]. Last modified May 16, 2006. Version 2. Checksum: 2C7FF0650E7E0F58	FASTA	267	28,542
	10 20 30 40 50 60 MSNKIAILLT VLIAVVACAR AQPSRRHPLV QPRSPHGSGH RIVGGFEINV SDTPYQVSLQ 70 80 90 100 110 120 YINSHRCGGS VLNSKWVLTA AHCTDGLQAF TLTVRLGSSR HASSGTVVNV ARIVEHPKYN 130 140 150 160 170 180 EYNTDYDYAL LELESELTFS DVVQPVALPE QDEAVDAGTM TIVSGWGSTK SATESNAILR 190 200 210 220 230 240 AANVPTVDQE ECREAYSHDA ITDRMLCAGY QQGGKDACQG DSGGPLVADG KLIGVVSWGS 250 260 GCAQPGYPGV YARVAVVRNW VREISGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Constitutive and blood meal-induced trypsin genes in Anopheles gambiae." Mueller H.-M., Catteruccia F., Vizioli J., della Torre A., Crisanti A. Exp. Parasitol. 81:371-385(1995) [PubMed: 7498434] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Suakoko. Tissue: Midgut.
[2]	"The genome sequence of the malaria mosquito Anopheles gambiae." Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L. Science 298:129-149(2002) [PubMed: 12364791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PEST.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z22930 Genomic DNA. Translation: CAA80516.1. AAAB01008964 Genomic DNA. Translation: EAA12262.3.
PIR	S40006.
RefSeq	XP_317172.2.
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
ModBase	Search...
Genome annotation databases
Ensembl	AGAP008293. Anopheles gambiae. [Contig view]
GeneID	1277689.
KEGG	aga:AgaP_AGAP008293.
VectorBase	AGAP008293. Anopheles gambiae.
Phylogenomic databases
HOGENOM	P35041.
Enzyme and pathway databases
BRENDA	3.4.21.4. 165157.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRY7_ANOGA

Accession

Primary (citable) accession number: P35041
Secondary accession number(s): Q7PNF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)