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Protein

Trypsin-7

Gene

TRYP7

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Constitutive trypsin that is expressed 2 days after emergence, coinciding with host seeking behavior of the female.1 Publication

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei82 – 821Charge relay systemBy similarity
Active sitei127 – 1271Charge relay systemBy similarity
Sitei216 – 2161Required for specificityBy similarity
Active sitei222 – 2221Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-7 (EC:3.4.21.4)
Gene namesi
Name:TRYP7
ORF Names:AGAP008293
OrganismiAnopheles gambiae (African malaria mosquito)
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
ProteomesiUP000007062 Componenti: Chromosome 3R

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Propeptidei19 – 4123Activation peptidePRO_0000028255Add
BLAST
Chaini42 – 267226Trypsin-7PRO_0000028256Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi67 ↔ 83PROSITE-ProRule annotation
Disulfide bondi192 ↔ 207PROSITE-ProRule annotation
Disulfide bondi218 ↔ 242PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Tissue specificityi

Expressed in the midgut. Expression levels drop a few hours after blood feeding and pick up again 28 hours later.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP35041.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 266225Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
InParanoidiP35041.
OMAiIDIRDAP.
OrthoDBiEOG7RNK07.
PhylomeDBiP35041.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35041-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKIAILLT VLIAVVACAR AQPSRRHPLV QPRSPHGSGH RIVGGFEINV
60 70 80 90 100
SDTPYQVSLQ YINSHRCGGS VLNSKWVLTA AHCTDGLQAF TLTVRLGSSR
110 120 130 140 150
HASSGTVVNV ARIVEHPKYN EYNTDYDYAL LELESELTFS DVVQPVALPE
160 170 180 190 200
QDEAVDAGTM TIVSGWGSTK SATESNAILR AANVPTVDQE ECREAYSHDA
210 220 230 240 250
ITDRMLCAGY QQGGKDACQG DSGGPLVADG KLIGVVSWGS GCAQPGYPGV
260
YARVAVVRNW VREISGV
Length:267
Mass (Da):28,542
Last modified:May 16, 2006 - v2
Checksum:i2C7FF0650E7E0F58
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti118 – 1247KYNEYNT → NYDDSTI in CAA80516 (PubMed:7498434).Curated
Sequence conflicti170 – 1734KSAT → HNAA in CAA80516 (PubMed:7498434).Curated
Sequence conflicti199 – 1991D → E in CAA80516 (PubMed:7498434).Curated
Sequence conflicti240 – 2401S → A in CAA80516 (PubMed:7498434).Curated
Sequence conflicti256 – 2561V → I in CAA80516 (PubMed:7498434).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22930 Genomic DNA. Translation: CAA80516.1.
AAAB01008964 Genomic DNA. Translation: EAA12262.3.
PIRiS40006.
RefSeqiXP_317172.2. XM_317172.4.

Genome annotation databases

EnsemblMetazoaiAGAP008293-RA; AGAP008293-PA; AGAP008293.
GeneIDi1277689.
KEGGiaga:AgaP_AGAP008293.
VectorBaseiAGAP008293. Anopheles gambiae.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22930 Genomic DNA. Translation: CAA80516.1.
AAAB01008964 Genomic DNA. Translation: EAA12262.3.
PIRiS40006.
RefSeqiXP_317172.2. XM_317172.4.

3D structure databases

ProteinModelPortaliP35041.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAGAP008293-RA; AGAP008293-PA; AGAP008293.
GeneIDi1277689.
KEGGiaga:AgaP_AGAP008293.
VectorBaseiAGAP008293. Anopheles gambiae.

Organism-specific databases

CTDi1277689.

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
InParanoidiP35041.
OMAiIDIRDAP.
OrthoDBiEOG7RNK07.
PhylomeDBiP35041.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Constitutive and blood meal-induced trypsin genes in Anopheles gambiae."
    Mueller H.-M., Catteruccia F., Vizioli J., della Torre A., Crisanti A.
    Exp. Parasitol. 81:371-385(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Suakoko.
    Tissue: Midgut.
  2. "The genome sequence of the malaria mosquito Anopheles gambiae."
    Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F.
    , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L.
    Science 298:129-149(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PEST.

Entry informationi

Entry nameiTRY7_ANOGA
AccessioniPrimary (citable) accession number: P35041
Secondary accession number(s): Q7PNF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 16, 2006
Last modified: March 4, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.