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P35040

- TRY6_ANOGA

UniProt

P35040 - TRY6_ANOGA

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Protein
Trypsin-6
Gene
TRYP6, AGAP008290
Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Constitutive trypsin that is expressed 2 days after emergence, coinciding with host seeking behavior of the female.1 Publication

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei87 – 871Charge relay system By similarity
Active sitei132 – 1321Charge relay system By similarity
Sitei222 – 2221Required for specificity By similarity
Active sitei228 – 2281Charge relay system By similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Protein family/group databases

MEROPSiS01.130.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-6 (EC:3.4.21.4)
Gene namesi
Name:TRYP6
ORF Names:AGAP008290
OrganismiAnopheles gambiae (African malaria mosquito)
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
ProteomesiUP000007062: Chromosome 3R

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed prediction
Add
BLAST
Propeptidei23 – 4624Activation peptide
PRO_0000028253Add
BLAST
Chaini47 – 273227Trypsin-6
PRO_0000028254Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi72 ↔ 88 By similarity
Disulfide bondi197 ↔ 213 By similarity
Disulfide bondi224 ↔ 248 By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Zymogen

Expressioni

Tissue specificityi

Expressed in the midgut. Expression levels drop a few hours after blood feeding and pick up again 28 hours later.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP35040.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 272226Peptidase S1
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
InParanoidiP35040.
OrthoDBiEOG75B84T.
PhylomeDBiP35040.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35040-1 [UniParc]FASTAAdd to Basket

« Hide

MLSKFTAILL AVHIALFACA LTQAEKRHKL TRPAFHPNAP YLAGKRIVGG    50
FVIDISDAPY QISLQYNGKH HCGGSILNSK WILTAAHCID LYSEVKPTVR 100
VGSSEHAAGG TVLHLLRIVP HPGHSSGGNN YDIALLELEC ELTFNDNVQP 150
VQLPEQDDPI DEGTMGIVSG WGMTMSAADL NAILRATNVP TVNQQECNQA 200
YQSYGGVAEQ MFCAGYKQGG TGTCRNDSGG PFVAEGKLIG VVSWSHECAL 250
AGYPGVYARV ASVRDWIRET SGV 273
Length:273
Mass (Da):29,295
Last modified:May 16, 2006 - v2
Checksum:i4A485528B7D679D1
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131H → L in CAA80513. 1 Publication
Sequence conflicti45 – 451K → R in CAA80513. 1 Publication
Sequence conflicti56 – 561S → A in CAA80513. 1 Publication
Sequence conflicti94 – 941E → Q in CAA80513. 1 Publication
Sequence conflicti116 – 1161L → V in CAA80513. 1 Publication
Sequence conflicti127 – 1282GG → SA in CAA80513. 1 Publication
Sequence conflicti140 – 1423CEL → SEI in CAA80513. 1 Publication
Sequence conflicti148 – 1481V → L in CAA80513. 1 Publication
Sequence conflicti152 – 1521Q → S in CAA80513. 1 Publication
Sequence conflicti161 – 1611D → E in CAA80513. 1 Publication
Sequence conflicti180 – 1801L → S in CAA80513. 1 Publication
Sequence conflicti207 – 2082VA → IT in CAA80513. 1 Publication
Sequence conflicti249 – 2491A → E in CAA80513. 1 Publication
Sequence conflicti265 – 2651D → E in CAA80513. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z22930 Genomic DNA. Translation: CAA80513.1.
AAAB01008964 Genomic DNA. Translation: EAA12587.2.
PIRiS40003.
RefSeqiXP_317175.2. XM_317175.2.

Genome annotation databases

EnsemblMetazoaiAGAP008290-RA; AGAP008290-PA; AGAP008290.
GeneIDi1277692.
KEGGiaga:AgaP_AGAP008290.
VectorBaseiAGAP008290. Anopheles gambiae.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z22930 Genomic DNA. Translation: CAA80513.1 .
AAAB01008964 Genomic DNA. Translation: EAA12587.2 .
PIRi S40003.
RefSeqi XP_317175.2. XM_317175.2.

3D structure databases

ProteinModelPortali P35040.
ModBasei Search...

Protein family/group databases

MEROPSi S01.130.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai AGAP008290-RA ; AGAP008290-PA ; AGAP008290 .
GeneIDi 1277692.
KEGGi aga:AgaP_AGAP008290.
VectorBasei AGAP008290. Anopheles gambiae.

Organism-specific databases

CTDi 1277692.

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000251820.
InParanoidi P35040.
OrthoDBi EOG75B84T.
PhylomeDBi P35040.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Constitutive and blood meal-induced trypsin genes in Anopheles gambiae."
    Mueller H.-M., Catteruccia F., Vizioli J., della Torre A., Crisanti A.
    Exp. Parasitol. 81:371-385(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Suakoko.
    Tissue: Midgut.
  2. "The genome sequence of the malaria mosquito Anopheles gambiae."
    Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F.
    , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L.
    Science 298:129-149(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PEST.

Entry informationi

Entry nameiTRY6_ANOGA
AccessioniPrimary (citable) accession number: P35040
Secondary accession number(s): Q7Q492
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 16, 2006
Last modified: September 3, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Caution

As this protein has Gly-222 in the position that determines the specificity of the enzyme instead of the Asp found in trypsins, it could have a chymotrypsin-like activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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