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P35040

- TRY6_ANOGA

UniProt

P35040 - TRY6_ANOGA

Protein

Trypsin-6

Gene

TRYP6

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 2 (16 May 2006)
      Previous versions | rss
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    Functioni

    Constitutive trypsin that is expressed 2 days after emergence, coinciding with host seeking behavior of the female.1 Publication

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei87 – 871Charge relay systemBy similarity
    Active sitei132 – 1321Charge relay systemBy similarity
    Sitei222 – 2221Required for specificityBy similarity
    Active sitei228 – 2281Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. digestion Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Protein family/group databases

    MEROPSiS01.130.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin-6 (EC:3.4.21.4)
    Gene namesi
    Name:TRYP6
    ORF Names:AGAP008290
    OrganismiAnopheles gambiae (African malaria mosquito)
    Taxonomic identifieri7165 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
    ProteomesiUP000007062: Chromosome 3R

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Propeptidei23 – 4624Activation peptidePRO_0000028253Add
    BLAST
    Chaini47 – 273227Trypsin-6PRO_0000028254Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi72 ↔ 88PROSITE-ProRule annotation
    Disulfide bondi197 ↔ 213PROSITE-ProRule annotation
    Disulfide bondi224 ↔ 248PROSITE-ProRule annotation

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Zymogen

    Expressioni

    Tissue specificityi

    Expressed in the midgut. Expression levels drop a few hours after blood feeding and pick up again 28 hours later.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP35040.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini47 – 272226Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    InParanoidiP35040.
    OrthoDBiEOG75B84T.
    PhylomeDBiP35040.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35040-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSKFTAILL AVHIALFACA LTQAEKRHKL TRPAFHPNAP YLAGKRIVGG    50
    FVIDISDAPY QISLQYNGKH HCGGSILNSK WILTAAHCID LYSEVKPTVR 100
    VGSSEHAAGG TVLHLLRIVP HPGHSSGGNN YDIALLELEC ELTFNDNVQP 150
    VQLPEQDDPI DEGTMGIVSG WGMTMSAADL NAILRATNVP TVNQQECNQA 200
    YQSYGGVAEQ MFCAGYKQGG TGTCRNDSGG PFVAEGKLIG VVSWSHECAL 250
    AGYPGVYARV ASVRDWIRET SGV 273
    Length:273
    Mass (Da):29,295
    Last modified:May 16, 2006 - v2
    Checksum:i4A485528B7D679D1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131H → L in CAA80513. (PubMed:7498434)Curated
    Sequence conflicti45 – 451K → R in CAA80513. (PubMed:7498434)Curated
    Sequence conflicti56 – 561S → A in CAA80513. (PubMed:7498434)Curated
    Sequence conflicti94 – 941E → Q in CAA80513. (PubMed:7498434)Curated
    Sequence conflicti116 – 1161L → V in CAA80513. (PubMed:7498434)Curated
    Sequence conflicti127 – 1282GG → SA in CAA80513. (PubMed:7498434)Curated
    Sequence conflicti140 – 1423CEL → SEI in CAA80513. (PubMed:7498434)Curated
    Sequence conflicti148 – 1481V → L in CAA80513. (PubMed:7498434)Curated
    Sequence conflicti152 – 1521Q → S in CAA80513. (PubMed:7498434)Curated
    Sequence conflicti161 – 1611D → E in CAA80513. (PubMed:7498434)Curated
    Sequence conflicti180 – 1801L → S in CAA80513. (PubMed:7498434)Curated
    Sequence conflicti207 – 2082VA → IT in CAA80513. (PubMed:7498434)Curated
    Sequence conflicti249 – 2491A → E in CAA80513. (PubMed:7498434)Curated
    Sequence conflicti265 – 2651D → E in CAA80513. (PubMed:7498434)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22930 Genomic DNA. Translation: CAA80513.1.
    AAAB01008964 Genomic DNA. Translation: EAA12587.2.
    PIRiS40003.
    RefSeqiXP_317175.2. XM_317175.2.

    Genome annotation databases

    EnsemblMetazoaiAGAP008290-RA; AGAP008290-PA; AGAP008290.
    GeneIDi1277692.
    KEGGiaga:AgaP_AGAP008290.
    VectorBaseiAGAP008290. Anopheles gambiae.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22930 Genomic DNA. Translation: CAA80513.1 .
    AAAB01008964 Genomic DNA. Translation: EAA12587.2 .
    PIRi S40003.
    RefSeqi XP_317175.2. XM_317175.2.

    3D structure databases

    ProteinModelPortali P35040.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.130.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai AGAP008290-RA ; AGAP008290-PA ; AGAP008290 .
    GeneIDi 1277692.
    KEGGi aga:AgaP_AGAP008290.
    VectorBasei AGAP008290. Anopheles gambiae.

    Organism-specific databases

    CTDi 1277692.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    InParanoidi P35040.
    OrthoDBi EOG75B84T.
    PhylomeDBi P35040.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Constitutive and blood meal-induced trypsin genes in Anopheles gambiae."
      Mueller H.-M., Catteruccia F., Vizioli J., della Torre A., Crisanti A.
      Exp. Parasitol. 81:371-385(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: Suakoko.
      Tissue: Midgut.
    2. "The genome sequence of the malaria mosquito Anopheles gambiae."
      Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F.
      , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L.
      Science 298:129-149(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PEST.

    Entry informationi

    Entry nameiTRY6_ANOGA
    AccessioniPrimary (citable) accession number: P35040
    Secondary accession number(s): Q7Q492
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 106 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Caution

    As this protein has Gly-222 in the position that determines the specificity of the enzyme instead of the Asp found in trypsins, it could have a chymotrypsin-like activity.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3