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Protein

Trypsin-5

Gene

TRYP5

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Constitutive trypsin that is expressed 2 days after emergence, coinciding with host seeking behavior of the female.1 Publication

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei88Charge relay systemBy similarity1
Active sitei132Charge relay systemBy similarity1
Sitei223Required for specificityBy similarity1
Active sitei229Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Protein family/group databases

MEROPSiS01.130.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-5 (EC:3.4.21.4)
Gene namesi
Name:TRYP5
ORF Names:AGAP008291
OrganismiAnopheles gambiae (African malaria mosquito)
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
Proteomesi
  • UP000007062 Componentsi: Chromosome 3R, Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000002825122 – 47Activation peptideAdd BLAST26
ChainiPRO_000002825248 – 274Trypsin-5Add BLAST227

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi73 ↔ 89PROSITE-ProRule annotation
Disulfide bondi197 ↔ 214PROSITE-ProRule annotation
Disulfide bondi225 ↔ 249PROSITE-ProRule annotation

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Zymogen

Proteomic databases

PaxDbiP35039.

Expressioni

Tissue specificityi

Expressed in the midgut. Expression levels drop a few hours after blood feeding and pick up again 28 hours later.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP35039.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 273Peptidase S1PROSITE-ProRule annotationAdd BLAST226

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
InParanoidiP35039.
OrthoDBiEOG091G0DF7.
PhylomeDBiP35039.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35039-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIKFTTLLA VLFALLAYAR AQAERRHKLT RPVHRFAPNR PYLAGKRIVG
60 70 80 90 100
GFVINISDAP YQISLQYDDD HNCGGSILSS KWILTAAHCI NDNAPSKPTV
110 120 130 140 150
RVGSSKHASG GTVIRVARIV PHPMHGSKNN YDIALLELKN ELTFSEKVQP
160 170 180 190 200
IALPEQDEPI EEGTMGIVSG WGLTLSEADS NDVLRATNVP TVNQQECNKA
210 220 230 240 250
YQSRYGGITD QMFCAGYKQG GQDTCRQDSG GPFVAKGKLI GVISWGHECA
260 270
LAGYPGVYAR VASVRDWIRT TSGV
Length:274
Mass (Da):29,815
Last modified:May 16, 2006 - v2
Checksum:i468AB656797AED8E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13F → I in CAA80514 (PubMed:7498434).Curated1
Sequence conflicti18Y → C in CAA80514 (PubMed:7498434).Curated1
Sequence conflicti55N → D in CAA80514 (PubMed:7498434).Curated1
Sequence conflicti106K → E in CAA80514 (PubMed:7498434).Curated1
Sequence conflicti114I → V in CAA80514 (PubMed:7498434).Curated1
Sequence conflicti180S → T in CAA80514 (PubMed:7498434).Curated1
Sequence conflicti211Q → E in CAA80514 (PubMed:7498434).Curated1
Sequence conflicti236K → E in CAA80514 (PubMed:7498434).Curated1
Sequence conflicti259A → P in CAA80514 (PubMed:7498434).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22930 Genomic DNA. Translation: CAA80514.1.
AAAB01008964 Genomic DNA. Translation: EAA12584.2.
PIRiS40004.
RefSeqiXP_317174.2. XM_317174.3.

Genome annotation databases

EnsemblMetazoaiAGAP008291-RA; AGAP008291-PA; AGAP008291.
GeneIDi1277691.
KEGGiaga:AgaP_AGAP008291.
VectorBaseiAGAP008291-RA; AGAP008291-PA; AGAP008291.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22930 Genomic DNA. Translation: CAA80514.1.
AAAB01008964 Genomic DNA. Translation: EAA12584.2.
PIRiS40004.
RefSeqiXP_317174.2. XM_317174.3.

3D structure databases

ProteinModelPortaliP35039.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.130.

Proteomic databases

PaxDbiP35039.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAGAP008291-RA; AGAP008291-PA; AGAP008291.
GeneIDi1277691.
KEGGiaga:AgaP_AGAP008291.
VectorBaseiAGAP008291-RA; AGAP008291-PA; AGAP008291.

Organism-specific databases

CTDi1277691.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
InParanoidiP35039.
OrthoDBiEOG091G0DF7.
PhylomeDBiP35039.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRY5_ANOGA
AccessioniPrimary (citable) accession number: P35039
Secondary accession number(s): Q7Q493
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 16, 2006
Last modified: November 30, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.