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P35039

- TRY5_ANOGA

UniProt

P35039 - TRY5_ANOGA

Protein

Trypsin-5

Gene

TRYP5

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (16 May 2006)
      Previous versions | rss
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    Functioni

    Constitutive trypsin that is expressed 2 days after emergence, coinciding with host seeking behavior of the female.1 Publication

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Charge relay systemBy similarity
    Active sitei132 – 1321Charge relay systemBy similarity
    Sitei223 – 2231Required for specificityBy similarity
    Active sitei229 – 2291Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. digestion Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Protein family/group databases

    MEROPSiS01.130.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin-5 (EC:3.4.21.4)
    Gene namesi
    Name:TRYP5
    ORF Names:AGAP008291
    OrganismiAnopheles gambiae (African malaria mosquito)
    Taxonomic identifieri7165 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
    ProteomesiUP000007062: Chromosome 3R

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Propeptidei22 – 4726Activation peptidePRO_0000028251Add
    BLAST
    Chaini48 – 274227Trypsin-5PRO_0000028252Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi73 ↔ 89PROSITE-ProRule annotation
    Disulfide bondi197 ↔ 214PROSITE-ProRule annotation
    Disulfide bondi225 ↔ 249PROSITE-ProRule annotation

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Zymogen

    Expressioni

    Tissue specificityi

    Expressed in the midgut. Expression levels drop a few hours after blood feeding and pick up again 28 hours later.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP35039.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini48 – 273226Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    InParanoidiP35039.
    OMAiCINDNAP.
    OrthoDBiEOG75B84T.
    PhylomeDBiP35039.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35039-1 [UniParc]FASTAAdd to Basket

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    MSIKFTTLLA VLFALLAYAR AQAERRHKLT RPVHRFAPNR PYLAGKRIVG    50
    GFVINISDAP YQISLQYDDD HNCGGSILSS KWILTAAHCI NDNAPSKPTV 100
    RVGSSKHASG GTVIRVARIV PHPMHGSKNN YDIALLELKN ELTFSEKVQP 150
    IALPEQDEPI EEGTMGIVSG WGLTLSEADS NDVLRATNVP TVNQQECNKA 200
    YQSRYGGITD QMFCAGYKQG GQDTCRQDSG GPFVAKGKLI GVISWGHECA 250
    LAGYPGVYAR VASVRDWIRT TSGV 274
    Length:274
    Mass (Da):29,815
    Last modified:May 16, 2006 - v2
    Checksum:i468AB656797AED8E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131F → I in CAA80514. (PubMed:7498434)Curated
    Sequence conflicti18 – 181Y → C in CAA80514. (PubMed:7498434)Curated
    Sequence conflicti55 – 551N → D in CAA80514. (PubMed:7498434)Curated
    Sequence conflicti106 – 1061K → E in CAA80514. (PubMed:7498434)Curated
    Sequence conflicti114 – 1141I → V in CAA80514. (PubMed:7498434)Curated
    Sequence conflicti180 – 1801S → T in CAA80514. (PubMed:7498434)Curated
    Sequence conflicti211 – 2111Q → E in CAA80514. (PubMed:7498434)Curated
    Sequence conflicti236 – 2361K → E in CAA80514. (PubMed:7498434)Curated
    Sequence conflicti259 – 2591A → P in CAA80514. (PubMed:7498434)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22930 Genomic DNA. Translation: CAA80514.1.
    AAAB01008964 Genomic DNA. Translation: EAA12584.2.
    PIRiS40004.
    RefSeqiXP_317174.2. XM_317174.3.

    Genome annotation databases

    EnsemblMetazoaiAGAP008291-RA; AGAP008291-PA; AGAP008291.
    GeneIDi1277691.
    KEGGiaga:AgaP_AGAP008291.
    VectorBaseiAGAP008291. Anopheles gambiae.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22930 Genomic DNA. Translation: CAA80514.1 .
    AAAB01008964 Genomic DNA. Translation: EAA12584.2 .
    PIRi S40004.
    RefSeqi XP_317174.2. XM_317174.3.

    3D structure databases

    ProteinModelPortali P35039.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.130.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai AGAP008291-RA ; AGAP008291-PA ; AGAP008291 .
    GeneIDi 1277691.
    KEGGi aga:AgaP_AGAP008291.
    VectorBasei AGAP008291. Anopheles gambiae.

    Organism-specific databases

    CTDi 1277691.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    InParanoidi P35039.
    OMAi CINDNAP.
    OrthoDBi EOG75B84T.
    PhylomeDBi P35039.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Constitutive and blood meal-induced trypsin genes in Anopheles gambiae."
      Mueller H.-M., Catteruccia F., Vizioli J., della Torre A., Crisanti A.
      Exp. Parasitol. 81:371-385(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: Suakoko.
      Tissue: Midgut.
    2. "The genome sequence of the malaria mosquito Anopheles gambiae."
      Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F.
      , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L.
      Science 298:129-149(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PEST.

    Entry informationi

    Entry nameiTRY5_ANOGA
    AccessioniPrimary (citable) accession number: P35039
    Secondary accession number(s): Q7Q493
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3