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P35039

- TRY5_ANOGA

UniProt

P35039 - TRY5_ANOGA

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Protein
Trypsin-5
Gene
TRYP5, AGAP008291
Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Constitutive trypsin that is expressed 2 days after emergence, coinciding with host seeking behavior of the female.1 Publication

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Charge relay system By similarity
Active sitei132 – 1321Charge relay system By similarity
Sitei223 – 2231Required for specificity By similarity
Active sitei229 – 2291Charge relay system By similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Protein family/group databases

MEROPSiS01.130.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-5 (EC:3.4.21.4)
Gene namesi
Name:TRYP5
ORF Names:AGAP008291
OrganismiAnopheles gambiae (African malaria mosquito)
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
ProteomesiUP000007062: Chromosome 3R

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Propeptidei22 – 4726Activation peptide
PRO_0000028251Add
BLAST
Chaini48 – 274227Trypsin-5
PRO_0000028252Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi73 ↔ 89 By similarity
Disulfide bondi197 ↔ 214 By similarity
Disulfide bondi225 ↔ 249 By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Zymogen

Expressioni

Tissue specificityi

Expressed in the midgut. Expression levels drop a few hours after blood feeding and pick up again 28 hours later.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP35039.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini48 – 273226Peptidase S1
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
InParanoidiP35039.
OMAiCINDNAP.
OrthoDBiEOG75B84T.
PhylomeDBiP35039.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35039-1 [UniParc]FASTAAdd to Basket

« Hide

MSIKFTTLLA VLFALLAYAR AQAERRHKLT RPVHRFAPNR PYLAGKRIVG    50
GFVINISDAP YQISLQYDDD HNCGGSILSS KWILTAAHCI NDNAPSKPTV 100
RVGSSKHASG GTVIRVARIV PHPMHGSKNN YDIALLELKN ELTFSEKVQP 150
IALPEQDEPI EEGTMGIVSG WGLTLSEADS NDVLRATNVP TVNQQECNKA 200
YQSRYGGITD QMFCAGYKQG GQDTCRQDSG GPFVAKGKLI GVISWGHECA 250
LAGYPGVYAR VASVRDWIRT TSGV 274
Length:274
Mass (Da):29,815
Last modified:May 16, 2006 - v2
Checksum:i468AB656797AED8E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131F → I in CAA80514. 1 Publication
Sequence conflicti18 – 181Y → C in CAA80514. 1 Publication
Sequence conflicti55 – 551N → D in CAA80514. 1 Publication
Sequence conflicti106 – 1061K → E in CAA80514. 1 Publication
Sequence conflicti114 – 1141I → V in CAA80514. 1 Publication
Sequence conflicti180 – 1801S → T in CAA80514. 1 Publication
Sequence conflicti211 – 2111Q → E in CAA80514. 1 Publication
Sequence conflicti236 – 2361K → E in CAA80514. 1 Publication
Sequence conflicti259 – 2591A → P in CAA80514. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z22930 Genomic DNA. Translation: CAA80514.1.
AAAB01008964 Genomic DNA. Translation: EAA12584.2.
PIRiS40004.
RefSeqiXP_317174.2. XM_317174.3.

Genome annotation databases

EnsemblMetazoaiAGAP008291-RA; AGAP008291-PA; AGAP008291.
GeneIDi1277691.
KEGGiaga:AgaP_AGAP008291.
VectorBaseiAGAP008291. Anopheles gambiae.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z22930 Genomic DNA. Translation: CAA80514.1 .
AAAB01008964 Genomic DNA. Translation: EAA12584.2 .
PIRi S40004.
RefSeqi XP_317174.2. XM_317174.3.

3D structure databases

ProteinModelPortali P35039.
ModBasei Search...

Protein family/group databases

MEROPSi S01.130.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai AGAP008291-RA ; AGAP008291-PA ; AGAP008291 .
GeneIDi 1277691.
KEGGi aga:AgaP_AGAP008291.
VectorBasei AGAP008291. Anopheles gambiae.

Organism-specific databases

CTDi 1277691.

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000251820.
InParanoidi P35039.
OMAi CINDNAP.
OrthoDBi EOG75B84T.
PhylomeDBi P35039.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Constitutive and blood meal-induced trypsin genes in Anopheles gambiae."
    Mueller H.-M., Catteruccia F., Vizioli J., della Torre A., Crisanti A.
    Exp. Parasitol. 81:371-385(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Suakoko.
    Tissue: Midgut.
  2. "The genome sequence of the malaria mosquito Anopheles gambiae."
    Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F.
    , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L.
    Science 298:129-149(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PEST.

Entry informationi

Entry nameiTRY5_ANOGA
AccessioniPrimary (citable) accession number: P35039
Secondary accession number(s): Q7Q493
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 16, 2006
Last modified: September 3, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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