Reviewed, UniProtKB/Swiss-Prot P35039 (TRY5_ANOGA)

Names and origin

Protein names

Recommended name:
    Trypsin-5
    EC=3.4.21.4

Gene names

Name:	TRYP5
ORF Names:	AGAP008291

Organism

Anopheles gambiae (African malaria mosquito) [Complete proteome]

Taxonomic identifier

7165 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Anophelinae › Anopheles

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Constitutive trypsin that is expressed 2 days after emergence, coinciding with host seeking behavior of the female. Ref.1
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted. Ref.1
Tissue specificity	Expressed in the midgut. Expression levels drop a few hours after blood feeding and pick up again 28 hours later. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Cleavage on pair of basic residues Disulfide bond Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 47	26	Activation peptide		PRO_0000028251
	Chain	48 – 274	227	Trypsin-5		PRO_0000028252
Regions
	Domain	48 – 273	226	Peptidase S1
Sites
	Active site	88	1	Charge relay system By similarity
	Active site	132	1	Charge relay system By similarity
	Active site	229	1	Charge relay system By similarity
	Site	223	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	73 ↔ 89		By similarity
	Disulfide bond	197 ↔ 214		By similarity
	Disulfide bond	225 ↔ 249		By similarity
Experimental info
	Sequence conflict	13	1	F → I in CAA80514. Ref.1
	Sequence conflict	18	1	Y → C in CAA80514. Ref.1
	Sequence conflict	55	1	N → D in CAA80514. Ref.1
	Sequence conflict	106	1	K → E in CAA80514. Ref.1
	Sequence conflict	114	1	I → V in CAA80514. Ref.1
	Sequence conflict	180	1	S → T in CAA80514. Ref.1
	Sequence conflict	211	1	Q → E in CAA80514. Ref.1
	Sequence conflict	236	1	K → E in CAA80514. Ref.1
	Sequence conflict	259	1	A → P in CAA80514. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P35039-1 [UniParc]. Last modified May 16, 2006. Version 2. Checksum: 468AB656797AED8E	FASTA	274	29,815
	10 20 30 40 50 60 MSIKFTTLLA VLFALLAYAR AQAERRHKLT RPVHRFAPNR PYLAGKRIVG GFVINISDAP 70 80 90 100 110 120 YQISLQYDDD HNCGGSILSS KWILTAAHCI NDNAPSKPTV RVGSSKHASG GTVIRVARIV 130 140 150 160 170 180 PHPMHGSKNN YDIALLELKN ELTFSEKVQP IALPEQDEPI EEGTMGIVSG WGLTLSEADS 190 200 210 220 230 240 NDVLRATNVP TVNQQECNKA YQSRYGGITD QMFCAGYKQG GQDTCRQDSG GPFVAKGKLI 250 260 270 GVISWGHECA LAGYPGVYAR VASVRDWIRT TSGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Constitutive and blood meal-induced trypsin genes in Anopheles gambiae." Mueller H.-M., Catteruccia F., Vizioli J., della Torre A., Crisanti A. Exp. Parasitol. 81:371-385(1995) [PubMed: 7498434] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Suakoko. Tissue: Midgut.
[2]	"The genome sequence of the malaria mosquito Anopheles gambiae." Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L. Science 298:129-149(2002) [PubMed: 12364791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PEST.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z22930 Genomic DNA. Translation: CAA80514.1. AAAB01008964 Genomic DNA. Translation: EAA12584.2.
PIR	S40004.
RefSeq	XP_317174.2.
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
ModBase	Search...
Protein family/group databases
MEROPS	S01.130.
Genome annotation databases
Ensembl	AGAP008291-RA; AGAP008291-PA; AGAP008291; Anopheles gambiae. [Genome view]
GeneID	1277691.
KEGG	aga:AgaP_AGAP008291.
VectorBase	AGAP008291. Anopheles gambiae.
Phylogenomic databases
HOGENOM	P35039.
Enzyme and pathway databases
BRENDA	3.4.21.4. 165157.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRY5_ANOGA

Accession

Primary (citable) accession number: P35039
Secondary accession number(s): Q7Q493

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	May 16, 2006
Last modified:	July 28, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)