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P35038

- TRY4_ANOGA

UniProt

P35038 - TRY4_ANOGA

Protein

Trypsin-4

Gene

TRYP4

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (16 May 2006)
      Previous versions | rss
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    Functioni

    Constitutive trypsin that is expressed 2 days after emergence, coinciding with host seeking behavior of the female.1 Publication

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei89 – 891Charge relay systemBy similarity
    Active sitei134 – 1341Charge relay systemBy similarity
    Sitei224 – 2241Required for specificityBy similarity
    Active sitei230 – 2301Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. digestion Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Protein family/group databases

    MEROPSiS01.130.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin-4 (EC:3.4.21.4)
    Gene namesi
    Name:TRYP4
    ORF Names:AGAP008292
    OrganismiAnopheles gambiae (African malaria mosquito)
    Taxonomic identifieri7165 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
    ProteomesiUP000007062: Chromosome 3R

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Propeptidei19 – 4830Activation peptidePRO_0000028249Add
    BLAST
    Chaini49 – 275227Trypsin-4PRO_0000028250Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi74 ↔ 90PROSITE-ProRule annotation
    Disulfide bondi199 ↔ 215PROSITE-ProRule annotation
    Disulfide bondi226 ↔ 250PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Expressioni

    Tissue specificityi

    Expressed in the midgut. Expression levels drop a few hours after blood feeding and pick up again 28 hours later.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP35038.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini49 – 274226Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    InParanoidiP35038.
    OMAiHCTELAS.
    OrthoDBiEOG7RNK07.
    PhylomeDBiP35038.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35038-1 [UniParc]FASTAAdd to Basket

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    MSNKITILLA VLLAVVACAQ AHASHQRRVP YPLPRFLPRP HHTVSNHRIV    50
    GGFEIDVAET PYQVSLQRSK RHICGGSVLS GKWILTAAHC TDGSQPASLT 100
    VRLGSSRHAS GGSVIHVARI VQHPDYDQET IDYDYSLLEL ESVLTFSNKV 150
    QPIALPEQDE AVEDGIMTIV SGWGSTKSAI ESNAILRAAN VPTVNQDECN 200
    QAYHKSEGIT ERMLCAGYQQ GGKDACQGDS GGPLVAEDKL IGVVSWGAGC 250
    AQPGYPGVYA RVAVVRDWIR ETCGV 275
    Length:275
    Mass (Da):29,608
    Last modified:May 16, 2006 - v2
    Checksum:iAEFFEDA9F24F71C8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti97 – 971A → E in CAA80515. (PubMed:7498434)Curated
    Sequence conflicti154 – 1541A → T in CAA80515. (PubMed:7498434)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22930 Genomic DNA. Translation: CAA80515.1.
    AAAB01008964 Genomic DNA. Translation: EAA12264.2.
    PIRiS40005.
    RefSeqiXP_317173.2. XM_317173.3.

    Genome annotation databases

    EnsemblMetazoaiAGAP008292-RA; AGAP008292-PA; AGAP008292.
    GeneIDi1277690.
    KEGGiaga:AgaP_AGAP008292.
    VectorBaseiAGAP008292. Anopheles gambiae.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22930 Genomic DNA. Translation: CAA80515.1 .
    AAAB01008964 Genomic DNA. Translation: EAA12264.2 .
    PIRi S40005.
    RefSeqi XP_317173.2. XM_317173.3.

    3D structure databases

    ProteinModelPortali P35038.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.130.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai AGAP008292-RA ; AGAP008292-PA ; AGAP008292 .
    GeneIDi 1277690.
    KEGGi aga:AgaP_AGAP008292.
    VectorBasei AGAP008292. Anopheles gambiae.

    Organism-specific databases

    CTDi 1277690.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    InParanoidi P35038.
    OMAi HCTELAS.
    OrthoDBi EOG7RNK07.
    PhylomeDBi P35038.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Constitutive and blood meal-induced trypsin genes in Anopheles gambiae."
      Mueller H.-M., Catteruccia F., Vizioli J., della Torre A., Crisanti A.
      Exp. Parasitol. 81:371-385(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: Suakoko.
      Tissue: Midgut.
    2. "The genome sequence of the malaria mosquito Anopheles gambiae."
      Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F.
      , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L.
      Science 298:129-149(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PEST.

    Entry informationi

    Entry nameiTRY4_ANOGA
    AccessioniPrimary (citable) accession number: P35038
    Secondary accession number(s): Q7Q494
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3