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Protein

Trypsin-4

Gene

TRYP4

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Constitutive trypsin that is expressed 2 days after emergence, coinciding with host seeking behavior of the female.1 Publication

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei89Charge relay systemBy similarity1
Active sitei134Charge relay systemBy similarity1
Sitei224Required for specificityBy similarity1
Active sitei230Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Protein family/group databases

MEROPSiS01.130.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-4 (EC:3.4.21.4)
Gene namesi
Name:TRYP4
ORF Names:AGAP008292
OrganismiAnopheles gambiae (African malaria mosquito)
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
Proteomesi
  • UP000007062 Componentsi: Chromosome 3R, Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
PropeptideiPRO_000002824919 – 48Activation peptideAdd BLAST30
ChainiPRO_000002825049 – 275Trypsin-4Add BLAST227

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi74 ↔ 90PROSITE-ProRule annotation
Disulfide bondi199 ↔ 215PROSITE-ProRule annotation
Disulfide bondi226 ↔ 250PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP35038.

Expressioni

Tissue specificityi

Expressed in the midgut. Expression levels drop a few hours after blood feeding and pick up again 28 hours later.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP35038.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 274Peptidase S1PROSITE-ProRule annotationAdd BLAST226

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
InParanoidiP35038.
OMAiHCTELAS.
OrthoDBiEOG091G0DF7.
PhylomeDBiP35038.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35038-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKITILLA VLLAVVACAQ AHASHQRRVP YPLPRFLPRP HHTVSNHRIV
60 70 80 90 100
GGFEIDVAET PYQVSLQRSK RHICGGSVLS GKWILTAAHC TDGSQPASLT
110 120 130 140 150
VRLGSSRHAS GGSVIHVARI VQHPDYDQET IDYDYSLLEL ESVLTFSNKV
160 170 180 190 200
QPIALPEQDE AVEDGIMTIV SGWGSTKSAI ESNAILRAAN VPTVNQDECN
210 220 230 240 250
QAYHKSEGIT ERMLCAGYQQ GGKDACQGDS GGPLVAEDKL IGVVSWGAGC
260 270
AQPGYPGVYA RVAVVRDWIR ETCGV
Length:275
Mass (Da):29,608
Last modified:May 16, 2006 - v2
Checksum:iAEFFEDA9F24F71C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti97A → E in CAA80515 (PubMed:7498434).Curated1
Sequence conflicti154A → T in CAA80515 (PubMed:7498434).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22930 Genomic DNA. Translation: CAA80515.1.
AAAB01008964 Genomic DNA. Translation: EAA12264.2.
PIRiS40005.
RefSeqiXP_317173.2. XM_317173.3.

Genome annotation databases

EnsemblMetazoaiAGAP008292-RA; AGAP008292-PA; AGAP008292.
GeneIDi1277690.
KEGGiaga:AgaP_AGAP008292.
VectorBaseiAGAP008292-RA; AGAP008292-PA; AGAP008292.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22930 Genomic DNA. Translation: CAA80515.1.
AAAB01008964 Genomic DNA. Translation: EAA12264.2.
PIRiS40005.
RefSeqiXP_317173.2. XM_317173.3.

3D structure databases

ProteinModelPortaliP35038.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.130.

Proteomic databases

PaxDbiP35038.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAGAP008292-RA; AGAP008292-PA; AGAP008292.
GeneIDi1277690.
KEGGiaga:AgaP_AGAP008292.
VectorBaseiAGAP008292-RA; AGAP008292-PA; AGAP008292.

Organism-specific databases

CTDi1277690.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
InParanoidiP35038.
OMAiHCTELAS.
OrthoDBiEOG091G0DF7.
PhylomeDBiP35038.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRY4_ANOGA
AccessioniPrimary (citable) accession number: P35038
Secondary accession number(s): Q7Q494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 16, 2006
Last modified: November 30, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.