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Reviewed, UniProtKB/Swiss-Prot P35038 (TRY4_ANOGA)

Last modified June 16, 2009. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Trypsin-4
    EC=3.4.21.4
Gene names
Name: TRYP4
ORF Names: AGAP008292
OrganismAnopheles gambiae (African malaria mosquito) [Complete proteome]
Taxonomic identifier7165 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Anophelinae › Anopheles

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Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Constitutive trypsin that is expressed 2 days after emergence, coinciding with host seeking behavior of the female. Ref.1

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Subcellular location

Secreted. Ref.1

Tissue specificity

Expressed in the midgut. Expression levels drop a few hours after blood feeding and pick up again 28 hours later. Ref.1

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

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Ontologies

Keywords
   Biological processDigestion
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Zymogen
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdigestion

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Propeptide19 – 4830Activation peptide
PRO_0000028249
Chain49 – 275227Trypsin-4
PRO_0000028250

Regions

Domain49 – 274226Peptidase S1

Sites

Active site891Charge relay system By similarity
Active site1341Charge relay system By similarity
Active site2301Charge relay system By similarity
Site2241Required for specificity By similarity

Amino acid modifications

Disulfide bond74 ↔ 90 By similarity
Disulfide bond199 ↔ 215 By similarity
Disulfide bond226 ↔ 250 By similarity

Experimental info

Sequence conflict971A → E in CAA80515. Ref.1
Sequence conflict1541A → T in CAA80515. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P35038-1 [UniParc].

Last modified May 16, 2006. Version 2.
Checksum: AEFFEDA9F24F71C8

FASTA27529,608
        10         20         30         40         50         60 
MSNKITILLA VLLAVVACAQ AHASHQRRVP YPLPRFLPRP HHTVSNHRIV GGFEIDVAET 

        70         80         90        100        110        120 
PYQVSLQRSK RHICGGSVLS GKWILTAAHC TDGSQPASLT VRLGSSRHAS GGSVIHVARI 

       130        140        150        160        170        180 
VQHPDYDQET IDYDYSLLEL ESVLTFSNKV QPIALPEQDE AVEDGIMTIV SGWGSTKSAI 

       190        200        210        220        230        240 
ESNAILRAAN VPTVNQDECN QAYHKSEGIT ERMLCAGYQQ GGKDACQGDS GGPLVAEDKL 

       250        260        270 
IGVVSWGAGC AQPGYPGVYA RVAVVRDWIR ETCGV

« Hide

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References

« Hide 'large scale' references
[1]"Constitutive and blood meal-induced trypsin genes in Anopheles gambiae."
Mueller H.-M., Catteruccia F., Vizioli J., della Torre A., Crisanti A.
Exp. Parasitol. 81:371-385(1995) [PubMed: 7498434] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: Suakoko.
Tissue: Midgut.
[2]"The genome sequence of the malaria mosquito Anopheles gambiae."
Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. expand/collapse author list , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L.
Science 298:129-149(2002) [PubMed: 12364791] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PEST.

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Cross-references

Sequence databases

Z22930 Genomic DNA. Translation: CAA80515.1.
AAAB01008964 Genomic DNA. Translation: EAA12264.2.
PIRS40005.
RefSeqXP_317173.2.

3D structure databases

HSSPHSSP built from PDB template 1EZX based on UniProtKB P00760.
ModBaseSearch...

Protein family/group databases

MEROPSS01.130.

Genome annotation databases

EnsemblAGAP008292. Anopheles gambiae. [Contig view]
GeneID1277690.
KEGGaga:AgaP_AGAP008292.
VectorBaseAGAP008292. Anopheles gambiae.

Phylogenomic databases

HOGENOMP35038.
OMAP35038. HCAGSSE.

Enzyme and pathway databases

BRENDA3.4.21.4. 165157.

Family and domain databases

InterProIPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameTRY4_ANOGA
AccessionPrimary (citable) accession number: P35038
Secondary accession number(s): Q7Q494
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 16, 2006
Last modified: June 16, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents