Reviewed, UniProtKB/Swiss-Prot P35037 (TRY3_ANOGA)

Names and origin

Protein names

Recommended name:
    Trypsin-3
    EC=3.4.21.4

Gene names

Name:	TRYP3
ORF Names:	AGAP008294

Organism

Anopheles gambiae (African malaria mosquito) [Complete proteome]

Taxonomic identifier

7165 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Anophelinae › Anopheles

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Constitutive trypsin that is expressed 2 days after emergence, coinciding with host seeking behavior of the female. Ref.1
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted Ref.1.
Tissue specificity	Expressed in the midgut. Expression levels drop a few hours after blood feeding and pick up again 28 hours later. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.
Sequence caution	The sequence EAA12261.3 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 48	26	Activation peptide		PRO_0000028247
	Chain	49 – 275	227	Trypsin-3		PRO_0000028248
Regions
	Domain	49 – 274	226	Peptidase S1
Sites
	Active site	89	1	Charge relay system By similarity
	Active site	134	1	Charge relay system By similarity
	Active site	230	1	Charge relay system By similarity
	Site	224	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	74 ↔ 90		By similarity
	Disulfide bond	199 ↔ 215		By similarity
	Disulfide bond	226 ↔ 250		By similarity
Experimental info
	Sequence conflict	42	1	K → Q in CAA80517. Ref.1
	Sequence conflict	142	1	S → T in CAA80517. Ref.1
	Sequence conflict	157	1	D → E in CAA80517. Ref.1
	Sequence conflict	169	1	I → T in CAA80517. Ref.1
	Sequence conflict	191	1	V → I in CAA80517. Ref.1
	Sequence conflict	267	1	D → N in CAA80517. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P35037-1 [UniParc]. Last modified July 1, 2008. Version 5. Checksum: 93D27A26AF4C5458	FASTA	275	29,374
	10 20 30 40 50 60 MISNKIAILL AVLVVAVACA QARVALKHRS VQALPRFLPR PKYDVGHRIV GGFEIDVSET 70 80 90 100 110 120 PYQVSLQYFN SHRCGGSVLN SKWILTAAHC TVNLQPSSLA VRLGSSRHAS GGTVVRVARV 130 140 150 160 170 180 LEHPNYDDST IDYDFSLMEL ESELTFSDVV QPVSLPDQDE AVEDGTMTIV SGWGNTQSAA 190 200 210 220 230 240 ESNAILRAAN VPTVNQKECT IAYSSSGGIT DRMLCAGYKR GGKDACQGDS GGPLVVDGKL 250 260 270 VGVVSWGFGC AMPGYPGVYA RVAVVRDWVR ENSGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Constitutive and blood meal-induced trypsin genes in Anopheles gambiae." Mueller H.-M., Catteruccia F., Vizioli J., della Torre A., Crisanti A. Exp. Parasitol. 81:371-385(1995) [PubMed: 7498434] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Suakoko. Tissue: Midgut.
[2]	"The genome sequence of the malaria mosquito Anopheles gambiae." Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L. Science 298:129-149(2002) [PubMed: 12364791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PEST.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z22930 Genomic DNA. Translation: CAA80517.1. AAAB01008964 Genomic DNA. Translation: EAA12261.3. Sequence problems.
PIR	S40007.
RefSeq	XP_317171.2.
3D structure databases
SMR	P35037. Positions 49-274.
ModBase	Search...
Protein family/group databases
MEROPS	S01.130.
Genome annotation databases
GeneID	3291693.
KEGG	aga:AgaP_AGAP008294.
VectorBase	AGAP008294. Anopheles gambiae.
Phylogenomic databases
eggNOG	inNOG07667.
HOGENOM	HBG755338.
InParanoid	P35037.
OrthoDB	EOG9BZN7X.
Enzyme and pathway databases
BRENDA	3.4.21.4. 165157.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR009003. Ser/Cys_Pept_Trypsin-like. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRY3_ANOGA

Accession

Primary (citable) accession number: P35037
Secondary accession number(s): Q7PNF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	July 1, 2008
Last modified:	January 19, 2010
This is version 77 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)