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P35036 (TRY2_ANOGA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trypsin-2

EC=3.4.21.4
Alternative name(s):
Antryp2
Gene names
Name:TRYP2
ORF Names:AGAP008295
OrganismAnopheles gambiae (African malaria mosquito) [Reference proteome]
Taxonomic identifier7165 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles

Protein attributes

Sequence length277 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Major function may be to aid in digestion of the blood meal. Ref.1

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Subcellular location

Secreted Ref.1.

Tissue specificity

Midgut. Ref.1

Induction

By blood meal. Ref.1

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Biological processDigestion
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdigestion

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Propeptide20 – 5031Activation peptide
PRO_0000028245
Chain51 – 277227Trypsin-2
PRO_0000028246

Regions

Domain51 – 276226Peptidase S1

Sites

Active site911Charge relay system By similarity
Active site1361Charge relay system By similarity
Active site2321Charge relay system By similarity
Site2261Required for specificity By similarity

Amino acid modifications

Disulfide bond76 ↔ 92 By similarity
Disulfide bond201 ↔ 217 By similarity
Disulfide bond228 ↔ 252 By similarity

Experimental info

Sequence conflict131V → L in CAA79328. Ref.1
Sequence conflict131V → L in CAA80518. Ref.2
Sequence conflict261R → G in CAA79328. Ref.1
Sequence conflict261R → G in CAA80518. Ref.2
Sequence conflict1351Y → F in CAA79328. Ref.1
Sequence conflict1351Y → F in CAA80518. Ref.2
Sequence conflict1511A → L in CAA79328. Ref.1
Sequence conflict1511A → L in CAA80518. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P35036 [UniParc].

Last modified May 16, 2006. Version 2.
Checksum: 7C1EAF6FA549B2E7

FASTA27729,822
        10         20         30         40         50         60 
MSNKIAILLL AVVVAVVACA QAQPSRRHHL VHPLLPRFLP RLHRDSNGHR VVGGFQIDVS 

        70         80         90        100        110        120 
DAPYQVSLQY FNSHRCGGSV LDNKWVLTAA HCTQGLDPSS LAVRLGSSEH ATGGTLVGVL 

       130        140        150        160        170        180 
RTVEHPQYDG NTIDYDFSLM ELETELTFSD AVQPVELPEH EEPVEPGTMA TVSGWGNTQS 

       190        200        210        220        230        240 
AVESSDFLRA ANVPTVSHED CSDAYMWFGE ITDRMLCAGY QQGGKDACQG DSGGPLVADG 

       250        260        270 
KLVGVVSWGY GCAQPGYPGV YGRVASVRDW VRENSGV 

« Hide

References

« Hide 'large scale' references
[1]"Members of a trypsin gene family in Anopheles gambiae are induced in the gut by blood meal."
Mueller H.-M., Crampton J.M., della Torre A., Sinden R., Crisanti A.
EMBO J. 12:2891-2900(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
Strain: Suakoko.
Tissue: Midgut.
[2]"Constitutive and blood meal-induced trypsin genes in Anopheles gambiae."
Mueller H.-M., Catteruccia F., Vizioli J., della Torre A., Crisanti A.
Exp. Parasitol. 81:371-385(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Suakoko.
Tissue: Midgut.
[3]"The genome sequence of the malaria mosquito Anopheles gambiae."
Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. expand/collapse author list , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L.
Science 298:129-149(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PEST.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z18890 mRNA. Translation: CAA79328.1.
Z22930 Genomic DNA. Translation: CAA80518.1.
AAAB01008964 Genomic DNA. Translation: EAL39600.1.
PIRS35340.

3D structure databases

ProteinModelPortalP35036.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.130.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaAGAP008295-RA; AGAP008295-PA; AGAP008295.
KEGGaga:AgaP_AGAP008295.
VectorBaseAGAP008295. Anopheles gambiae.

Organism-specific databases

CTD3291694.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
InParanoidP35036.
OMAWIEESTG.
OrthoDBEOG75B84T.
PhylomeDBP35036.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRY2_ANOGA
AccessionPrimary (citable) accession number: P35036
Secondary accession number(s): Q5TQD2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 16, 2006
Last modified: April 16, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries