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Protein

Trypsin-2

Gene

TRYP2

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Major function may be to aid in digestion of the blood meal.1 Publication

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei91Charge relay systemBy similarity1
Active sitei136Charge relay systemBy similarity1
Sitei226Required for specificityBy similarity1
Active sitei232Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Protein family/group databases

MEROPSiS01.130.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-2 (EC:3.4.21.4)
Alternative name(s):
Antryp2
Gene namesi
Name:TRYP2
ORF Names:AGAP008295
OrganismiAnopheles gambiae (African malaria mosquito)
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
Proteomesi
  • UP000007062 Componentsi: Chromosome 3R, Unassembled WGS sequence

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000002824520 – 50Activation peptideAdd BLAST31
ChainiPRO_000002824651 – 277Trypsin-2Add BLAST227

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi76 ↔ 92PROSITE-ProRule annotation
Disulfide bondi201 ↔ 217PROSITE-ProRule annotation
Disulfide bondi228 ↔ 252PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP35036.

Expressioni

Tissue specificityi

Midgut.1 Publication

Inductioni

By blood meal.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi7165.AGAP008295-PA.

Structurei

3D structure databases

ProteinModelPortaliP35036.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 276Peptidase S1PROSITE-ProRule annotationAdd BLAST226

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
InParanoidiP35036.
OMAiTVSHEDC.
OrthoDBiEOG091G0DF7.
PhylomeDBiP35036.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35036-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKIAILLL AVVVAVVACA QAQPSRRHHL VHPLLPRFLP RLHRDSNGHR
60 70 80 90 100
VVGGFQIDVS DAPYQVSLQY FNSHRCGGSV LDNKWVLTAA HCTQGLDPSS
110 120 130 140 150
LAVRLGSSEH ATGGTLVGVL RTVEHPQYDG NTIDYDFSLM ELETELTFSD
160 170 180 190 200
AVQPVELPEH EEPVEPGTMA TVSGWGNTQS AVESSDFLRA ANVPTVSHED
210 220 230 240 250
CSDAYMWFGE ITDRMLCAGY QQGGKDACQG DSGGPLVADG KLVGVVSWGY
260 270
GCAQPGYPGV YGRVASVRDW VRENSGV
Length:277
Mass (Da):29,822
Last modified:May 16, 2006 - v2
Checksum:i7C1EAF6FA549B2E7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13V → L in CAA79328 (PubMed:8335004).Curated1
Sequence conflicti13V → L in CAA80518 (PubMed:7498434).Curated1
Sequence conflicti26R → G in CAA79328 (PubMed:8335004).Curated1
Sequence conflicti26R → G in CAA80518 (PubMed:7498434).Curated1
Sequence conflicti135Y → F in CAA79328 (PubMed:8335004).Curated1
Sequence conflicti135Y → F in CAA80518 (PubMed:7498434).Curated1
Sequence conflicti151A → L in CAA79328 (PubMed:8335004).Curated1
Sequence conflicti151A → L in CAA80518 (PubMed:7498434).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18890 mRNA. Translation: CAA79328.1.
Z22930 Genomic DNA. Translation: CAA80518.1.
AAAB01008964 Genomic DNA. Translation: EAL39600.1.
PIRiS35340.
RefSeqiXP_555167.1. XM_555167.2.

Genome annotation databases

EnsemblMetazoaiAGAP008295-RA; AGAP008295-PA; AGAP008295.
GeneIDi3291694.
KEGGiaga:AgaP_AGAP008295.
VectorBaseiAGAP008295-RA; AGAP008295-PA; AGAP008295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18890 mRNA. Translation: CAA79328.1.
Z22930 Genomic DNA. Translation: CAA80518.1.
AAAB01008964 Genomic DNA. Translation: EAL39600.1.
PIRiS35340.
RefSeqiXP_555167.1. XM_555167.2.

3D structure databases

ProteinModelPortaliP35036.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7165.AGAP008295-PA.

Protein family/group databases

MEROPSiS01.130.

Proteomic databases

PaxDbiP35036.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAGAP008295-RA; AGAP008295-PA; AGAP008295.
GeneIDi3291694.
KEGGiaga:AgaP_AGAP008295.
VectorBaseiAGAP008295-RA; AGAP008295-PA; AGAP008295.

Organism-specific databases

CTDi3291694.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
InParanoidiP35036.
OMAiTVSHEDC.
OrthoDBiEOG091G0DF7.
PhylomeDBiP35036.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRY2_ANOGA
AccessioniPrimary (citable) accession number: P35036
Secondary accession number(s): Q5TQD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 16, 2006
Last modified: November 30, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.