Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P35036

- TRY2_ANOGA

UniProt

P35036 - TRY2_ANOGA

Protein

Trypsin-2

Gene

TRYP2

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (16 May 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Major function may be to aid in digestion of the blood meal.1 Publication

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei91 – 911Charge relay systemBy similarity
    Active sitei136 – 1361Charge relay systemBy similarity
    Sitei226 – 2261Required for specificityBy similarity
    Active sitei232 – 2321Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. digestion Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Protein family/group databases

    MEROPSiS01.130.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin-2 (EC:3.4.21.4)
    Alternative name(s):
    Antryp2
    Gene namesi
    Name:TRYP2
    ORF Names:AGAP008295
    OrganismiAnopheles gambiae (African malaria mosquito)
    Taxonomic identifieri7165 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
    ProteomesiUP000007062: Chromosome 3R

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Propeptidei20 – 5031Activation peptidePRO_0000028245Add
    BLAST
    Chaini51 – 277227Trypsin-2PRO_0000028246Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi76 ↔ 92PROSITE-ProRule annotation
    Disulfide bondi201 ↔ 217PROSITE-ProRule annotation
    Disulfide bondi228 ↔ 252PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Expressioni

    Tissue specificityi

    Midgut.1 Publication

    Inductioni

    By blood meal.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP35036.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini51 – 276226Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    InParanoidiP35036.
    OMAiWIEESTG.
    OrthoDBiEOG75B84T.
    PhylomeDBiP35036.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35036-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNKIAILLL AVVVAVVACA QAQPSRRHHL VHPLLPRFLP RLHRDSNGHR    50
    VVGGFQIDVS DAPYQVSLQY FNSHRCGGSV LDNKWVLTAA HCTQGLDPSS 100
    LAVRLGSSEH ATGGTLVGVL RTVEHPQYDG NTIDYDFSLM ELETELTFSD 150
    AVQPVELPEH EEPVEPGTMA TVSGWGNTQS AVESSDFLRA ANVPTVSHED 200
    CSDAYMWFGE ITDRMLCAGY QQGGKDACQG DSGGPLVADG KLVGVVSWGY 250
    GCAQPGYPGV YGRVASVRDW VRENSGV 277
    Length:277
    Mass (Da):29,822
    Last modified:May 16, 2006 - v2
    Checksum:i7C1EAF6FA549B2E7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131V → L in CAA79328. (PubMed:8335004)Curated
    Sequence conflicti13 – 131V → L in CAA80518. (PubMed:7498434)Curated
    Sequence conflicti26 – 261R → G in CAA79328. (PubMed:8335004)Curated
    Sequence conflicti26 – 261R → G in CAA80518. (PubMed:7498434)Curated
    Sequence conflicti135 – 1351Y → F in CAA79328. (PubMed:8335004)Curated
    Sequence conflicti135 – 1351Y → F in CAA80518. (PubMed:7498434)Curated
    Sequence conflicti151 – 1511A → L in CAA79328. (PubMed:8335004)Curated
    Sequence conflicti151 – 1511A → L in CAA80518. (PubMed:7498434)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z18890 mRNA. Translation: CAA79328.1.
    Z22930 Genomic DNA. Translation: CAA80518.1.
    AAAB01008964 Genomic DNA. Translation: EAL39600.1.
    PIRiS35340.
    RefSeqiXP_555167.1. XM_555167.2.

    Genome annotation databases

    EnsemblMetazoaiAGAP008295-RA; AGAP008295-PA; AGAP008295.
    GeneIDi3291694.
    KEGGiaga:AgaP_AGAP008295.
    VectorBaseiAGAP008295. Anopheles gambiae.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z18890 mRNA. Translation: CAA79328.1 .
    Z22930 Genomic DNA. Translation: CAA80518.1 .
    AAAB01008964 Genomic DNA. Translation: EAL39600.1 .
    PIRi S35340.
    RefSeqi XP_555167.1. XM_555167.2.

    3D structure databases

    ProteinModelPortali P35036.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.130.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai AGAP008295-RA ; AGAP008295-PA ; AGAP008295 .
    GeneIDi 3291694.
    KEGGi aga:AgaP_AGAP008295.
    VectorBasei AGAP008295. Anopheles gambiae.

    Organism-specific databases

    CTDi 3291694.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    InParanoidi P35036.
    OMAi WIEESTG.
    OrthoDBi EOG75B84T.
    PhylomeDBi P35036.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Members of a trypsin gene family in Anopheles gambiae are induced in the gut by blood meal."
      Mueller H.-M., Crampton J.M., della Torre A., Sinden R., Crisanti A.
      EMBO J. 12:2891-2900(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
      Strain: Suakoko.
      Tissue: Midgut.
    2. "Constitutive and blood meal-induced trypsin genes in Anopheles gambiae."
      Mueller H.-M., Catteruccia F., Vizioli J., della Torre A., Crisanti A.
      Exp. Parasitol. 81:371-385(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Suakoko.
      Tissue: Midgut.
    3. "The genome sequence of the malaria mosquito Anopheles gambiae."
      Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F.
      , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L.
      Science 298:129-149(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PEST.

    Entry informationi

    Entry nameiTRY2_ANOGA
    AccessioniPrimary (citable) accession number: P35036
    Secondary accession number(s): Q5TQD2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: May 16, 2006
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3