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P35035

- TRY1_ANOGA

UniProt

P35035 - TRY1_ANOGA

Protein

Trypsin-1

Gene

TRYP1

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 3 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Major function may be to aid in digestion of the blood meal.2 Publications

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Charge relay systemBy similarity
    Active sitei133 – 1331Charge relay systemBy similarity
    Sitei223 – 2231Required for specificityBy similarity
    Active sitei229 – 2291Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. digestion Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Protein family/group databases

    MEROPSiS01.130.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin-1 (EC:3.4.21.4)
    Alternative name(s):
    Antryp1
    Gene namesi
    Name:TRYP1
    ORF Names:AGAP008296
    OrganismiAnopheles gambiae (African malaria mosquito)
    Taxonomic identifieri7165 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
    ProteomesiUP000007062: Chromosome 3R

    Subcellular locationi

    Secreted 2 Publications

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Propeptidei19 – 4729Activation peptidePRO_0000028243Add
    BLAST
    Chaini48 – 274227Trypsin-1PRO_0000028244Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi73 ↔ 89PROSITE-ProRule annotation
    Disulfide bondi198 ↔ 214PROSITE-ProRule annotation
    Disulfide bondi225 ↔ 249PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Expressioni

    Tissue specificityi

    Constitutively expressed at low level in the gut of adult females. Also expressed in the gut of male and female pupae.2 Publications

    Inductioni

    By blood meal.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP35035.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini48 – 273226Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    InParanoidiP35035.
    KOiK01312.
    OMAiPASADWI.
    OrthoDBiEOG75B84T.
    PhylomeDBiP35035.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35035-1 [UniParc]FASTAAdd to Basket

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    MSNKIAILLA VLVAVVACAE AQANQRHRLV RPSPSFSPRP RYAVGQRIVG    50
    GFEIDVSDAP YQVSLQYNKR HNCGGSVLSS KWVLTAAHCT AGASPSSLTV 100
    RLGTSRHASG GTVVRVARVV QHPKYDSSSI DFDYSLLELE DELTFSDSVQ 150
    PVGLPKQDET VKDGTMTTVS GWGNTQSAAE SNAVLRAANV PTVNQKECNK 200
    AYSEFGGVTD RMLCAGYQQG GKDACQGDSG GPLVADGKLV GVVSWGYGCA 250
    QAGYPGVYSR VAVVRDWVRE NSGV 274
    Length:274
    Mass (Da):29,057
    Last modified:February 26, 2008 - v3
    Checksum:i7055CA51C6CEF3F8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti93 – 931A → R in CAA79327. (PubMed:8335004)Curated
    Sequence conflicti95 – 951P → T in CAA79327. (PubMed:8335004)Curated
    Sequence conflicti95 – 951P → T in CAA80512. (PubMed:7498434)Curated
    Sequence conflicti101 – 1011R → P in CAA79327. (PubMed:8335004)Curated
    Sequence conflicti148 – 1481S → A in CAA80512. (PubMed:7498434)Curated
    Sequence conflicti204 – 2041E → D in CAA79327. (PubMed:8335004)Curated
    Sequence conflicti204 – 2041E → D in CAA80512. (PubMed:7498434)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z18889 mRNA. Translation: CAA79327.1.
    Z22930 Genomic DNA. Translation: CAA80512.1.
    AAAB01008964 Genomic DNA. Translation: EAA12590.3.
    PIRiS35339.
    RefSeqiXP_317170.2. XM_317170.2.

    Genome annotation databases

    EnsemblMetazoaiAGAP008296-RA; AGAP008296-PA; AGAP008296.
    GeneIDi1277688.
    KEGGiaga:AgaP_AGAP008296.
    VectorBaseiAGAP008296. Anopheles gambiae.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z18889 mRNA. Translation: CAA79327.1 .
    Z22930 Genomic DNA. Translation: CAA80512.1 .
    AAAB01008964 Genomic DNA. Translation: EAA12590.3 .
    PIRi S35339.
    RefSeqi XP_317170.2. XM_317170.2.

    3D structure databases

    ProteinModelPortali P35035.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.130.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai AGAP008296-RA ; AGAP008296-PA ; AGAP008296 .
    GeneIDi 1277688.
    KEGGi aga:AgaP_AGAP008296.
    VectorBasei AGAP008296. Anopheles gambiae.

    Organism-specific databases

    CTDi 1277688.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    InParanoidi P35035.
    KOi K01312.
    OMAi PASADWI.
    OrthoDBi EOG75B84T.
    PhylomeDBi P35035.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Members of a trypsin gene family in Anopheles gambiae are induced in the gut by blood meal."
      Mueller H.-M., Crampton J.M., della Torre A., Sinden R., Crisanti A.
      EMBO J. 12:2891-2900(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
      Strain: Suakoko.
      Tissue: Midgut.
    2. "Constitutive and blood meal-induced trypsin genes in Anopheles gambiae."
      Mueller H.-M., Catteruccia F., Vizioli J., della Torre A., Crisanti A.
      Exp. Parasitol. 81:371-385(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: Suakoko.
      Tissue: Midgut.
    3. "The genome sequence of the malaria mosquito Anopheles gambiae."
      Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F.
      , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L.
      Science 298:129-149(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PEST.

    Entry informationi

    Entry nameiTRY1_ANOGA
    AccessioniPrimary (citable) accession number: P35035
    Secondary accession number(s): Q7PN85
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 106 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3