Reviewed, UniProtKB/Swiss-Prot P35035 (TRY1_ANOGA)

Names and origin

Protein names

Recommended name:
    Trypsin-1
    EC=3.4.21.4
Alternative name(s):
    Antryp1

Gene names

Name:	TRYP1
ORF Names:	AGAP008296

Organism

Anopheles gambiae (African malaria mosquito) [Complete proteome]

Taxonomic identifier

7165 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Anophelinae › Anopheles

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Major function may be to aid in digestion of the blood meal. Ref.1 Ref.2
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted. Ref.1 Ref.2
Tissue specificity	Constitutively expressed at low level in the gut of adult females. Also expressed in the gut of male and female pupae. Ref.1 Ref.2
Induction	By blood meal. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 47	29	Activation peptide		PRO_0000028243
	Chain	48 – 274	227	Trypsin-1		PRO_0000028244
Regions
	Domain	48 – 273	226	Peptidase S1
Sites
	Active site	88	1	Charge relay system By similarity
	Active site	133	1	Charge relay system By similarity
	Active site	229	1	Charge relay system By similarity
	Site	223	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	73 ↔ 89		By similarity
	Disulfide bond	198 ↔ 214		By similarity
	Disulfide bond	225 ↔ 249		By similarity
Experimental info
	Sequence conflict	93	1	A → R in CAA79327. Ref.1
	Sequence conflict	95	1	P → T in CAA79327. Ref.1
	Sequence conflict	95	1	P → T in CAA80512. Ref.2
	Sequence conflict	101	1	R → P in CAA79327. Ref.1
	Sequence conflict	148	1	S → A in CAA80512. Ref.2
	Sequence conflict	204	1	E → D in CAA79327. Ref.1
	Sequence conflict	204	1	E → D in CAA80512. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P35035-1 [UniParc]. Last modified February 26, 2008. Version 3. Checksum: 7055CA51C6CEF3F8	FASTA	274	29,057
	10 20 30 40 50 60 MSNKIAILLA VLVAVVACAE AQANQRHRLV RPSPSFSPRP RYAVGQRIVG GFEIDVSDAP 70 80 90 100 110 120 YQVSLQYNKR HNCGGSVLSS KWVLTAAHCT AGASPSSLTV RLGTSRHASG GTVVRVARVV 130 140 150 160 170 180 QHPKYDSSSI DFDYSLLELE DELTFSDSVQ PVGLPKQDET VKDGTMTTVS GWGNTQSAAE 190 200 210 220 230 240 SNAVLRAANV PTVNQKECNK AYSEFGGVTD RMLCAGYQQG GKDACQGDSG GPLVADGKLV 250 260 270 GVVSWGYGCA QAGYPGVYSR VAVVRDWVRE NSGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Members of a trypsin gene family in Anopheles gambiae are induced in the gut by blood meal." Mueller H.-M., Crampton J.M., della Torre A., Sinden R., Crisanti A. EMBO J. 12:2891-2900(1993) [PubMed: 8335004] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION. Strain: Suakoko. Tissue: Midgut.
[2]	"Constitutive and blood meal-induced trypsin genes in Anopheles gambiae." Mueller H.-M., Catteruccia F., Vizioli J., della Torre A., Crisanti A. Exp. Parasitol. 81:371-385(1995) [PubMed: 7498434] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Suakoko. Tissue: Midgut.
[3]	"The genome sequence of the malaria mosquito Anopheles gambiae." Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L. Science 298:129-149(2002) [PubMed: 12364791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PEST.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z18889 mRNA. Translation: CAA79327.1. Z22930 Genomic DNA. Translation: CAA80512.1. AAAB01008964 Genomic DNA. Translation: EAA12590.3.
PIR	S35339.
RefSeq	XP_317170.2.
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
ModBase	Search...
Protein family/group databases
MEROPS	S01.130.
Genome annotation databases
Ensembl	AGAP008296. Anopheles gambiae. [Contig view]
GeneID	1277688.
KEGG	aga:AgaP_AGAP008296.
VectorBase	AGAP008296. Anopheles gambiae.
Phylogenomic databases
HOGENOM	P35035.
OMA	P35035. YDSSSID.
Enzyme and pathway databases
BRENDA	3.4.21.4. 165157.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRY1_ANOGA

Accession

Primary (citable) accession number: P35035
Secondary accession number(s): Q7PN85

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)