Reviewed, UniProtKB/Swiss-Prot P35034 (TRYP_PLEPL)

Names and origin

Protein names	Recommended name:     Trypsin     EC=3.4.21.4
Organism	Pleuronectes platessa (Plaice)
Taxonomic identifier	8262 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Pleuronectiformes › Pleuronectoidei › Pleuronectidae › Pleuronectinae › Pleuronectes

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Potential
	Propeptide	16 – 22	7	Activation peptide		PRO_0000028237
	Chain	23 – 250	228	Trypsin		PRO_0000028238
Regions
	Domain	23 – 247	225	Peptidase S1
Sites
	Active site	62	1	Charge relay system By similarity
	Active site	106	1	Charge relay system By similarity
	Active site	203	1	Charge relay system By similarity
	Site	197	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	29 ↔ 163		By similarity
	Disulfide bond	47 ↔ 63		By similarity
	Disulfide bond	133 ↔ 236		By similarity
	Disulfide bond	140 ↔ 209		By similarity
	Disulfide bond	174 ↔ 188		By similarity
	Disulfide bond	199 ↔ 223		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35034-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 637DE96185C1ABAA	FASTA	250	27,528
	10 20 30 40 50 60 MRLLALLLMV GAAVAVPRED GRIIGGHECA AHSRPFMASL NYGYHFCGGV LINNQWVLSV 70 80 90 100 110 120 AHCWYNPYAM QVMLGEHDLR KFEGTEQLMK TDTIIWHPNY DYQTLDLTSC SSSSTILWKV 130 140 150 160 170 180 THAVAPIPLP TSCPVAGTPC SVSGWGNTAR DGDEVYLPTL LQCMDVPIVD EEQCMKSYPD 190 200 210 220 230 240 MISPRMVCAG FMDGSRDACN GDSGSPLVCR GEVYGLVSWG QGCAQPNYPG VYVKLCEFLG 250 WIERTLEAYP

References

[1]	Leaver M.J., George S.G. Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X56744 mRNA. Translation: CAA40068.1.
PIR	S31384.
3D structure databases
HSSP	HSSP built from PDB template 3BTE based on UniProtKB P00760.
ModBase	Search...
Protein family/group databases
MEROPS	S01.124.
Phylogenomic databases
HOVERGEN	P35034.
Enzyme and pathway databases
BRENDA	3.4.21.4. 74156.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. False negative. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYP_PLEPL

Accession

Primary (citable) accession number: P35034

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)