Reviewed, UniProtKB/Swiss-Prot P35033 (TRY3_SALSA)

Names and origin

Protein names	Recommended name:     Trypsin-3     EC=3.4.21.4 Alternative name(s):     Trypsin III
Organism	Salmo salar (Atlantic salmon)
Taxonomic identifier	8030 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Protacanthopterygii › Salmoniformes › Salmonidae › Salmoninae › Salmo

Protein attributes

Sequence length	238 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	3D-structure
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

‹1 – 7

›7

Potential

Propeptide

8 – 15

8

Activation peptide

PRO_0000028229

Chain

16 – 238

223

Trypsin-3

PRO_0000028230

Regions

Domain

16 – 236

221

Peptidase S1

Sites

Active site

55

1

Charge relay system By similarity

Active site

99

1

Charge relay system By similarity

Active site

192

1

Charge relay system By similarity

Metal binding

67

1

Calcium By similarity

Metal binding

69

1

Calcium; via carbonyl oxygen By similarity

Metal binding

72

1

Calcium; via carbonyl oxygen

Metal binding

77

1

Calcium By similarity

Site

186

1

Required for specificity By similarity

Amino acid modifications

Disulfide bond

22 ↔ 152

Disulfide bond

40 ↔ 56

Disulfide bond

124 ↔ 225

Disulfide bond

131 ↔ 198

Disulfide bond

163 ↔ 177

Disulfide bond

188 ↔ 212

Experimental info

Non-terminal residue

1

1

Secondary structure

1

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238

Helix Strand Turn

Details...

Turn

26 – 29

4

Beta strand

30 – 35

6

Beta strand

38 – 52

15

Helix

54 – 56

3

Beta strand

62 – 66

5

Beta strand

68 – 72

5

Beta strand

78 – 87

10

Turn

93 – 96

4

Beta strand

101 – 107

7

Beta strand

130 – 137

8

Beta strand

141 – 143

3

Beta strand

151 – 157

7

Helix

160 – 166

7

Turn

168 – 170

3

Beta strand

175 – 179

5

Beta strand

195 – 198

4

Beta strand

201 – 208

8

Beta strand

210 – 213

4

Beta strand

219 – 223

5

Helix

224 – 227

4

Helix

228 – 237

10

Sequences

	Sequence		Length	Mass (Da)
	P35033-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: AE799B80E8393023	FASTA	238	25,389
	10 20 30 40 50 60 FAVAFAAPID DEDDKIVGGY ECRKNSASYQ ASLQSGYHFC GGSLISSTWV VSAAHCYKSR 70 80 90 100 110 120 IQVRLGEHNI AVNEGTEQFI DSVKVIMHPS YNSRNLDNDI MLIKLSKPAS LNSYVSTVAL 130 140 150 160 170 180 PSSCASSGTR CLVSGWGNLS GSSSNYPDTL RCLDLPILSS SSCNSAYPGQ ITSNMFCAGF 190 200 210 220 230 MEGGKDSCQG DSGGPVVCNG QLQGVVSWGY GCAQRNKPGV YTKVCNYRSW ISSTMSSN

References

[1]	"Molecular cloning and characterization of anionic and cationic variants of trypsin from Atlantic salmon." Male R., Lorens J.B., Smals A.O., Torrissen K.R. Eur. J. Biochem. 232:677-685(1995) [PubMed: 7556223] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X70074 mRNA. Translation: CAA49679.1.

PIR

S31779. S66657.

UniGene

Ssa.23520

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A0J	X-ray	1.70	A/B/C/D	16-238	[»]

ModBase

Search...

Protein family/group databases

MEROPS

S01.126.

Phylogenomic databases

HOVERGEN

P35033.

Enzyme and pathway databases

BRENDA

3.4.21.4. 39345.

Family and domain databases

InterPro

IPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
[Graphical view]

Pfam

PF00089. Trypsin. 1 hit.
[Graphical view]

PRINTS

PR00722. CHYMOTRYPSIN.

SMART

SM00020. Tryp_SPc. 1 hit.
[Graphical view]

PROSITE

PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TRY3_SALSA

Accession

Primary (citable) accession number: P35033

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)