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Protein

Trypsin-3

Gene
N/A
Organism
Salmo salar (Atlantic salmon)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei55Charge relay systemBy similarity1
Metal bindingi67CalciumBy similarity1
Metal bindingi69Calcium; via carbonyl oxygenBy similarity1
Metal bindingi72Calcium; via carbonyl oxygen1
Metal bindingi77CalciumBy similarity1
Active sitei99Charge relay systemBy similarity1
Sitei186Required for specificityBy similarity1
Active sitei192Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiS01.126.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-3 (EC:3.4.21.4)
Alternative name(s):
Trypsin III
OrganismiSalmo salar (Atlantic salmon)
Taxonomic identifieri8030 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeSalmo

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei‹1 – 7Sequence analysis›7
PropeptideiPRO_00000282298 – 15Activation peptide8
ChainiPRO_000002823016 – 238Trypsin-3Add BLAST223

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi22 ↔ 152
Disulfide bondi40 ↔ 56
Disulfide bondi124 ↔ 225
Disulfide bondi131 ↔ 198
Disulfide bondi163 ↔ 177
Disulfide bondi188 ↔ 212

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PRIDEiP35033.

Structurei

Secondary structure

1238
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni26 – 29Combined sources4
Beta strandi30 – 35Combined sources6
Beta strandi38 – 52Combined sources15
Helixi54 – 56Combined sources3
Beta strandi62 – 66Combined sources5
Beta strandi68 – 72Combined sources5
Beta strandi78 – 87Combined sources10
Turni93 – 96Combined sources4
Beta strandi101 – 107Combined sources7
Beta strandi130 – 137Combined sources8
Beta strandi141 – 143Combined sources3
Beta strandi151 – 157Combined sources7
Helixi160 – 166Combined sources7
Turni168 – 170Combined sources3
Beta strandi175 – 179Combined sources5
Beta strandi195 – 198Combined sources4
Beta strandi201 – 208Combined sources8
Beta strandi210 – 213Combined sources4
Beta strandi219 – 223Combined sources5
Helixi224 – 227Combined sources4
Helixi228 – 237Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A0JX-ray1.70A/B/C/D16-238[»]
ProteinModelPortaliP35033.
SMRiP35033.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35033.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 236Peptidase S1PROSITE-ProRule annotationAdd BLAST221

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35033-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
FAVAFAAPID DEDDKIVGGY ECRKNSASYQ ASLQSGYHFC GGSLISSTWV
60 70 80 90 100
VSAAHCYKSR IQVRLGEHNI AVNEGTEQFI DSVKVIMHPS YNSRNLDNDI
110 120 130 140 150
MLIKLSKPAS LNSYVSTVAL PSSCASSGTR CLVSGWGNLS GSSSNYPDTL
160 170 180 190 200
RCLDLPILSS SSCNSAYPGQ ITSNMFCAGF MEGGKDSCQG DSGGPVVCNG
210 220 230
QLQGVVSWGY GCAQRNKPGV YTKVCNYRSW ISSTMSSN
Length:238
Mass (Da):25,389
Last modified:February 1, 1994 - v1
Checksum:iAE799B80E8393023
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70074 mRNA. Translation: CAA49679.1.
PIRiS66657. S31779.
UniGeneiSsa.23520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70074 mRNA. Translation: CAA49679.1.
PIRiS66657. S31779.
UniGeneiSsa.23520.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A0JX-ray1.70A/B/C/D16-238[»]
ProteinModelPortaliP35033.
SMRiP35033.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.126.

Proteomic databases

PRIDEiP35033.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG013304.

Miscellaneous databases

EvolutionaryTraceiP35033.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRY3_SALSA
AccessioniPrimary (citable) accession number: P35033
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.