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P35033

- TRY3_SALSA

UniProt

P35033 - TRY3_SALSA

Protein

Trypsin-3

Gene
N/A
Organism
Salmo salar (Atlantic salmon)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei55 – 551Charge relay systemBy similarity
    Metal bindingi67 – 671CalciumBy similarity
    Metal bindingi69 – 691Calcium; via carbonyl oxygenBy similarity
    Metal bindingi72 – 721Calcium; via carbonyl oxygen
    Metal bindingi77 – 771CalciumBy similarity
    Active sitei99 – 991Charge relay systemBy similarity
    Sitei186 – 1861Required for specificityBy similarity
    Active sitei192 – 1921Charge relay systemBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. digestion Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    MEROPSiS01.126.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin-3 (EC:3.4.21.4)
    Alternative name(s):
    Trypsin III
    OrganismiSalmo salar (Atlantic salmon)
    Taxonomic identifieri8030 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeSalmo

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei‹1 – 7›7Sequence Analysis
    Propeptidei8 – 158Activation peptidePRO_0000028229
    Chaini16 – 238223Trypsin-3PRO_0000028230Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi22 ↔ 152
    Disulfide bondi40 ↔ 56
    Disulfide bondi124 ↔ 225
    Disulfide bondi131 ↔ 198
    Disulfide bondi163 ↔ 177
    Disulfide bondi188 ↔ 212

    Keywords - PTMi

    Disulfide bond, Zymogen

    Structurei

    Secondary structure

    1
    238
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni26 – 294
    Beta strandi30 – 356
    Beta strandi38 – 5215
    Helixi54 – 563
    Beta strandi62 – 665
    Beta strandi68 – 725
    Beta strandi78 – 8710
    Turni93 – 964
    Beta strandi101 – 1077
    Beta strandi130 – 1378
    Beta strandi141 – 1433
    Beta strandi151 – 1577
    Helixi160 – 1667
    Turni168 – 1703
    Beta strandi175 – 1795
    Beta strandi195 – 1984
    Beta strandi201 – 2088
    Beta strandi210 – 2134
    Beta strandi219 – 2235
    Helixi224 – 2274
    Helixi228 – 23710

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1A0JX-ray1.70A/B/C/D16-238[»]
    ProteinModelPortaliP35033.
    SMRiP35033. Positions 16-238.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP35033.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 236221Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG013304.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35033-1 [UniParc]FASTAAdd to Basket

    « Hide

    FAVAFAAPID DEDDKIVGGY ECRKNSASYQ ASLQSGYHFC GGSLISSTWV    50
    VSAAHCYKSR IQVRLGEHNI AVNEGTEQFI DSVKVIMHPS YNSRNLDNDI 100
    MLIKLSKPAS LNSYVSTVAL PSSCASSGTR CLVSGWGNLS GSSSNYPDTL 150
    RCLDLPILSS SSCNSAYPGQ ITSNMFCAGF MEGGKDSCQG DSGGPVVCNG 200
    QLQGVVSWGY GCAQRNKPGV YTKVCNYRSW ISSTMSSN 238
    Length:238
    Mass (Da):25,389
    Last modified:February 1, 1994 - v1
    Checksum:iAE799B80E8393023
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70074 mRNA. Translation: CAA49679.1.
    PIRiS66657. S31779.
    UniGeneiSsa.23520.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70074 mRNA. Translation: CAA49679.1 .
    PIRi S66657. S31779.
    UniGenei Ssa.23520.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1A0J X-ray 1.70 A/B/C/D 16-238 [» ]
    ProteinModelPortali P35033.
    SMRi P35033. Positions 16-238.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.126.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG013304.

    Miscellaneous databases

    EvolutionaryTracei P35033.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of anionic and cationic variants of trypsin from Atlantic salmon."
      Male R., Lorens J.B., Smals A.O., Torrissen K.R.
      Eur. J. Biochem. 232:677-685(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pancreas.

    Entry informationi

    Entry nameiTRY3_SALSA
    AccessioniPrimary (citable) accession number: P35033
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3