P35032 (TRY2_SALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Trypsin-2 EC=3.4.21.4 Alternative name(s): Trypsin II
Organism	Salmo salar (Atlantic salmon)
Taxonomic identifier	8030 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Protacanthopterygii › Salmoniformes › Salmonoidei › Salmonidae › Salmoninae › Salmo

Protein attributes

Sequence length	231 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological_process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	‹1 – 4	›4	Potential
	Propeptide	5 – 9	5	Activation peptide		PRO_0000028227
	Chain	10 – 231	222	Trypsin-2		PRO_0000028228
Regions
	Domain	10 – 229	220	Peptidase S1
Sites
	Active site	49	1	Charge relay system By similarity
	Active site	93	1	Charge relay system By similarity
	Active site	185	1	Charge relay system By similarity
	Metal binding	61	1	Calcium By similarity
	Metal binding	63	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	66	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	71	1	Calcium By similarity
	Site	179	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	16 ↔ 145		By similarity
	Disulfide bond	34 ↔ 50		By similarity
	Disulfide bond	118 ↔ 218		By similarity
	Disulfide bond	125 ↔ 191		By similarity
	Disulfide bond	156 ↔ 170		By similarity
	Disulfide bond	181 ↔ 205		By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P35032 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: C54A1CAFE74FAE18	FASTA	231	24,823
	10 20 30 40 50 60 AAFATEDDKI VGGYECKAYS QPHQVSLNSG YHFCGGSLVN ENWVVSAAHC YQSRVEVRLG 70 80 90 100 110 120 EHNIQVTEGS EQFISSSRVI RHPNYSSYNI DNDIMLIKLS KPATLNTYVQ PVALPTSCAP 130 140 150 160 170 180 AGTMCTVSGW GNTMSSTADK NKLQCLNIPI LSYSDCNNSY PGMITNAMFC AGYLEGGKDS 190 200 210 220 230 CQGDSGGPVV CNGELQGVVS WGYGCAEPGN PGVYAKVCIF NDWLTSTMAT Y

References

[1]	"Molecular cloning and characterization of anionic and cationic variants of trypsin from Atlantic salmon." Male R., Lorens J.B., Smals A.O., Torrissen K.R. Eur. J. Biochem. 232:677-685(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X70073 mRNA. Translation: CAA49678.1.
PIR	S31778. S66658.
UniGene	Ssa.19782.
3D structure databases
ProteinModelPortal	P35032.
SMR	P35032. Positions 10-231.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG013304.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRY2_SALSA

Accession

Primary (citable) accession number: P35032

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program