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Protein

Trypsin-2

Gene
N/A
Organism
Salmo salar (Atlantic salmon)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei49 – 491Charge relay systemBy similarity
Metal bindingi61 – 611CalciumBy similarity
Metal bindingi63 – 631Calcium; via carbonyl oxygenBy similarity
Metal bindingi66 – 661Calcium; via carbonyl oxygenBy similarity
Metal bindingi71 – 711CalciumBy similarity
Active sitei93 – 931Charge relay systemBy similarity
Sitei179 – 1791Required for specificityBy similarity
Active sitei185 – 1851Charge relay systemBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-2 (EC:3.4.21.4)
Alternative name(s):
Trypsin II
OrganismiSalmo salar (Atlantic salmon)
Taxonomic identifieri8030 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeSalmo

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 4›4Sequence Analysis
Propeptidei5 – 95Activation peptidePRO_0000028227
Chaini10 – 231222Trypsin-2PRO_0000028228Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi16 ↔ 145PROSITE-ProRule annotation
Disulfide bondi34 ↔ 50PROSITE-ProRule annotation
Disulfide bondi118 ↔ 218PROSITE-ProRule annotation
Disulfide bondi125 ↔ 191PROSITE-ProRule annotation
Disulfide bondi156 ↔ 170PROSITE-ProRule annotation
Disulfide bondi181 ↔ 205PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP35032.
SMRiP35032. Positions 10-231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 229220Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35032-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
AAFATEDDKI VGGYECKAYS QPHQVSLNSG YHFCGGSLVN ENWVVSAAHC
60 70 80 90 100
YQSRVEVRLG EHNIQVTEGS EQFISSSRVI RHPNYSSYNI DNDIMLIKLS
110 120 130 140 150
KPATLNTYVQ PVALPTSCAP AGTMCTVSGW GNTMSSTADK NKLQCLNIPI
160 170 180 190 200
LSYSDCNNSY PGMITNAMFC AGYLEGGKDS CQGDSGGPVV CNGELQGVVS
210 220 230
WGYGCAEPGN PGVYAKVCIF NDWLTSTMAT Y
Length:231
Mass (Da):24,823
Last modified:January 31, 1994 - v1
Checksum:iC54A1CAFE74FAE18
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70073 mRNA. Translation: CAA49678.1.
PIRiS66658. S31778.
UniGeneiSsa.19782.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70073 mRNA. Translation: CAA49678.1.
PIRiS66658. S31778.
UniGeneiSsa.19782.

3D structure databases

ProteinModelPortaliP35032.
SMRiP35032. Positions 10-231.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of anionic and cationic variants of trypsin from Atlantic salmon."
    Male R., Lorens J.B., Smals A.O., Torrissen K.R.
    Eur. J. Biochem. 232:677-685(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pancreas.

Entry informationi

Entry nameiTRY2_SALSA
AccessioniPrimary (citable) accession number: P35032
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1994
Last sequence update: January 31, 1994
Last modified: January 6, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.