Reviewed, UniProtKB/Swiss-Prot P35031 (TRY1_SALSA)

Names and origin

Protein names	Recommended name:     Trypsin-1     EC=3.4.21.4 Alternative name(s):     Trypsin I
Organism	Salmo salar (Atlantic salmon)
Taxonomic identifier	8030 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Protacanthopterygii › Salmoniformes › Salmonidae › Salmoninae › Salmo

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	3D-structure
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 15

15

Potential

Propeptide

16 – 20

5

Activation peptide

PRO_0000028225

Chain

21 – 242

222

Trypsin-1

PRO_0000028226

Regions

Domain

21 – 240

220

Peptidase S1

Sites

Active site

60

1

Charge relay system

Active site

104

1

Charge relay system

Active site

196

1

Charge relay system

Metal binding

72

1

Calcium

Metal binding

74

1

Calcium; via carbonyl oxygen

Metal binding

77

1

Calcium; via carbonyl oxygen

Metal binding

82

1

Calcium

Site

190

1

Required for specificity By similarity

Amino acid modifications

Disulfide bond

27 ↔ 156

Disulfide bond

45 ↔ 61

Disulfide bond

129 ↔ 229

Disulfide bond

136 ↔ 202

Disulfide bond

167 ↔ 181

Disulfide bond

192 ↔ 216

Natural variations

Natural variant

33

1

T → A in trypsins IA/IB.

Secondary structure

1

.

.

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242

Helix Strand Turn

Details...

Beta strand

35 – 51

17

Beta strand

54 – 57

4

Helix

59 – 61

3

Beta strand

67 – 71

5

Beta strand

83 – 92

10

Turn

98 – 101

4

Beta strand

106 – 112

7

Beta strand

117 – 119

3

Beta strand

135 – 142

8

Beta strand

155 – 161

7

Helix

164 – 170

7

Beta strand

179 – 183

5

Beta strand

199 – 202

4

Beta strand

205 – 212

8

Beta strand

214 – 217

4

Beta strand

223 – 227

5

Helix

228 – 231

4

Helix

232 – 240

9

Sequences

	Sequence		Length	Mass (Da)
	P35031-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 43F5642498067E5A	FASTA	242	25,958
	10 20 30 40 50 60 MISLVFVLLI GAAFATEDDK IVGGYECKAY SQTHQVSLNS GYHFCGGSLV NENWVVSAAH 70 80 90 100 110 120 CYKSRVEVRL GEHNIKVTEG SEQFISSSRV IRHPNYSSYN IDNDIMLIKL SKPATLNTYV 130 140 150 160 170 180 QPVALPTSCA PAGTMCTVSG WGNTMSSTAD SNKLQCLNIP ILSYSDCNNS YPGMITNAMF 190 200 210 220 230 240 CAGYLEGGKD SCQGDSGGPV VCNGELQGVV SWGYGCAEPG NPGVYAKVCI FNDWLTSTMA SY

References

[1]	"Molecular cloning and characterization of anionic and cationic variants of trypsin from Atlantic salmon." Male R., Lorens J.B., Smals A.O., Torrissen K.R. Eur. J. Biochem. 232:677-685(1995) [PubMed: 7556223] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.
[2]	"Structure determination and refinement of benzamidine-inhibited trypsin from the North Atlantic salmon (Salmo salar) at 1.82-A resolution." Smalas A.O., Hordvik A. Acta Crystallogr. D 49:318-330(1993) [PubMed: 15299521] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS).
[3]	"Cold adaption of enzymes: structural comparison between salmon and bovine trypsins." Smalas A.O., Heimstad E.S., Hordvik A., Willassen N.P., Male R. Proteins 20:149-166(1994) [PubMed: 7846025] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X70075 mRNA. Translation: CAA49680.1.
X70071 mRNA. Translation: CAA49676.1.
X70072 mRNA. Translation: CAA49677.1.

PIR

S31776. S66659.
S31775. S66660.

RefSeq

NP_001117183.1.
NP_001119704.1.

UniGene

Ssa.38456
Ssa.628

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BIT	X-ray	1.83	A	6-242	[»]
1BZX	X-ray	2.10	E	21-242	[»]
1HJ8	X-ray	1.00	A	21-242	[»]
1UTJ	X-ray	1.83	A	1-242	[»]
1UTK	X-ray	1.53	A	1-242	[»]
1UTL	X-ray	1.70	M	1-242	[»]
1UTM	X-ray	1.50	A	1-242	[»]
2STA	X-ray	1.80	E	21-242	[»]
2STB	X-ray	1.80	E	21-242	[»]
2TBS	X-ray	1.80	A	21-242	[»]

ModBase

Search...

Protein family/group databases

MEROPS

S01.125.

Genome annotation databases

GeneID

100137021.
100137022.

Organism-specific databases

CTD

100137021.
100137022.

Phylogenomic databases

HOVERGEN

P35031.

Enzyme and pathway databases

BRENDA

3.4.21.4. 39345.

Family and domain databases

InterPro

IPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
IPR009003. Ser/Cys_Pept_Trypsin-like.
[Graphical view]

Pfam

PF00089. Trypsin. 1 hit.
[Graphical view]

PRINTS

PR00722. CHYMOTRYPSIN.

SMART

SM00020. Tryp_SPc. 1 hit.
[Graphical view]

PROSITE

PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TRY1_SALSA

Accession

Primary (citable) accession number: P35031

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	December 15, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)