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P35031

- TRY1_SALSA

UniProt

P35031 - TRY1_SALSA

Protein

Trypsin-1

Gene
N/A
Organism
Salmo salar (Atlantic salmon)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Cofactori

    Binds 1 calcium ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei60 – 601Charge relay system
    Metal bindingi72 – 721Calcium
    Metal bindingi74 – 741Calcium; via carbonyl oxygen
    Metal bindingi77 – 771Calcium; via carbonyl oxygen
    Metal bindingi82 – 821Calcium
    Active sitei104 – 1041Charge relay system
    Sitei190 – 1901Required for specificityBy similarity
    Active sitei196 – 1961Charge relay system

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. digestion Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    MEROPSiS01.125.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin-1 (EC:3.4.21.4)
    Alternative name(s):
    Trypsin I
    OrganismiSalmo salar (Atlantic salmon)
    Taxonomic identifieri8030 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeSalmo

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1515Sequence AnalysisAdd
    BLAST
    Propeptidei16 – 205Activation peptidePRO_0000028225
    Chaini21 – 242222Trypsin-1PRO_0000028226Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi27 ↔ 156
    Disulfide bondi45 ↔ 61
    Disulfide bondi129 ↔ 229
    Disulfide bondi136 ↔ 202
    Disulfide bondi167 ↔ 181
    Disulfide bondi192 ↔ 216

    Keywords - PTMi

    Disulfide bond, Zymogen

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-88002.

    Structurei

    Secondary structure

    1
    242
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni29 – 346
    Beta strandi35 – 5117
    Beta strandi54 – 574
    Helixi59 – 613
    Beta strandi67 – 715
    Beta strandi83 – 9210
    Turni98 – 1014
    Beta strandi106 – 1127
    Beta strandi117 – 1193
    Beta strandi135 – 1428
    Beta strandi146 – 1483
    Beta strandi155 – 1617
    Helixi164 – 1707
    Turni172 – 1743
    Beta strandi179 – 1835
    Turni193 – 1975
    Beta strandi199 – 2024
    Beta strandi205 – 2128
    Beta strandi214 – 2174
    Beta strandi223 – 2275
    Helixi228 – 2314
    Helixi232 – 2409

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BITX-ray1.83A6-242[»]
    1BZXX-ray2.10E21-242[»]
    1HJ8X-ray1.00A21-242[»]
    1UTJX-ray1.83A1-242[»]
    1UTKX-ray1.53A1-242[»]
    1UTLX-ray1.70M1-242[»]
    1UTMX-ray1.50A1-242[»]
    2STAX-ray1.80E21-242[»]
    2STBX-ray1.80E21-242[»]
    2TBSX-ray1.80A21-242[»]
    ProteinModelPortaliP35031.
    SMRiP35031. Positions 21-242.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP35031.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 240220Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG013304.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35031-1 [UniParc]FASTAAdd to Basket

    « Hide

    MISLVFVLLI GAAFATEDDK IVGGYECKAY SQTHQVSLNS GYHFCGGSLV    50
    NENWVVSAAH CYKSRVEVRL GEHNIKVTEG SEQFISSSRV IRHPNYSSYN 100
    IDNDIMLIKL SKPATLNTYV QPVALPTSCA PAGTMCTVSG WGNTMSSTAD 150
    SNKLQCLNIP ILSYSDCNNS YPGMITNAMF CAGYLEGGKD SCQGDSGGPV 200
    VCNGELQGVV SWGYGCAEPG NPGVYAKVCI FNDWLTSTMA SY 242
    Length:242
    Mass (Da):25,958
    Last modified:February 1, 1994 - v1
    Checksum:i43F5642498067E5A
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti33 – 331T → A in trypsins IA/IB.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70075 mRNA. Translation: CAA49680.1.
    X70071 mRNA. Translation: CAA49676.1.
    X70072 mRNA. Translation: CAA49677.1.
    PIRiS66659. S31776.
    S66660. S31775.
    RefSeqiNP_001117183.1. NM_001123711.1.
    NP_001119704.1. NM_001126232.1.
    UniGeneiSsa.38456.
    Ssa.628.

    Genome annotation databases

    GeneIDi100137021.
    100137022.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70075 mRNA. Translation: CAA49680.1 .
    X70071 mRNA. Translation: CAA49676.1 .
    X70072 mRNA. Translation: CAA49677.1 .
    PIRi S66659. S31776.
    S66660. S31775.
    RefSeqi NP_001117183.1. NM_001123711.1.
    NP_001119704.1. NM_001126232.1.
    UniGenei Ssa.38456.
    Ssa.628.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BIT X-ray 1.83 A 6-242 [» ]
    1BZX X-ray 2.10 E 21-242 [» ]
    1HJ8 X-ray 1.00 A 21-242 [» ]
    1UTJ X-ray 1.83 A 1-242 [» ]
    1UTK X-ray 1.53 A 1-242 [» ]
    1UTL X-ray 1.70 M 1-242 [» ]
    1UTM X-ray 1.50 A 1-242 [» ]
    2STA X-ray 1.80 E 21-242 [» ]
    2STB X-ray 1.80 E 21-242 [» ]
    2TBS X-ray 1.80 A 21-242 [» ]
    ProteinModelPortali P35031.
    SMRi P35031. Positions 21-242.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-88002.

    Protein family/group databases

    MEROPSi S01.125.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100137021.
    100137022.

    Organism-specific databases

    CTDi 100137021.
    100137022.

    Phylogenomic databases

    HOVERGENi HBG013304.

    Miscellaneous databases

    EvolutionaryTracei P35031.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of anionic and cationic variants of trypsin from Atlantic salmon."
      Male R., Lorens J.B., Smals A.O., Torrissen K.R.
      Eur. J. Biochem. 232:677-685(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pancreas.
    2. "Structure determination and refinement of benzamidine-inhibited trypsin from the North Atlantic salmon (Salmo salar) at 1.82-A resolution."
      Smalas A.O., Hordvik A.
      Acta Crystallogr. D 49:318-330(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS).
    3. "Cold adaption of enzymes: structural comparison between salmon and bovine trypsins."
      Smalas A.O., Heimstad E.S., Hordvik A., Willassen N.P., Male R.
      Proteins 20:149-166(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS).

    Entry informationi

    Entry nameiTRY1_SALSA
    AccessioniPrimary (citable) accession number: P35031
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3