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Protein

Trypsin-1

Gene
N/A
Organism
Salmo salar (Atlantic salmon)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Ca2+Note: Binds 1 Ca2+ ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei60Charge relay system1
Metal bindingi72Calcium1
Metal bindingi74Calcium; via carbonyl oxygen1
Metal bindingi77Calcium; via carbonyl oxygen1
Metal bindingi82Calcium1
Active sitei104Charge relay system1
Sitei190Required for specificityBy similarity1
Active sitei196Charge relay system1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiS01.125.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-1 (EC:3.4.21.4)
Alternative name(s):
Trypsin I
OrganismiSalmo salar (Atlantic salmon)
Taxonomic identifieri8030 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeSalmo

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Sequence analysisAdd BLAST15
PropeptideiPRO_000002822516 – 20Activation peptide5
ChainiPRO_000002822621 – 242Trypsin-1Add BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi27 ↔ 156
Disulfide bondi45 ↔ 61
Disulfide bondi129 ↔ 229
Disulfide bondi136 ↔ 202
Disulfide bondi167 ↔ 181
Disulfide bondi192 ↔ 216

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PRIDEiP35031.

Interactioni

Protein-protein interaction databases

MINTiMINT-88002.

Structurei

Secondary structure

1242
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni29 – 34Combined sources6
Beta strandi35 – 51Combined sources17
Beta strandi54 – 57Combined sources4
Helixi59 – 61Combined sources3
Beta strandi67 – 71Combined sources5
Beta strandi83 – 92Combined sources10
Turni98 – 101Combined sources4
Beta strandi106 – 112Combined sources7
Beta strandi117 – 119Combined sources3
Beta strandi135 – 142Combined sources8
Beta strandi146 – 148Combined sources3
Beta strandi155 – 161Combined sources7
Helixi164 – 170Combined sources7
Turni172 – 174Combined sources3
Beta strandi179 – 183Combined sources5
Turni193 – 197Combined sources5
Beta strandi199 – 202Combined sources4
Beta strandi205 – 212Combined sources8
Beta strandi214 – 217Combined sources4
Beta strandi223 – 227Combined sources5
Helixi228 – 231Combined sources4
Helixi232 – 240Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BITX-ray1.83A6-242[»]
1BZXX-ray2.10E21-242[»]
1HJ8X-ray1.00A21-242[»]
1UTJX-ray1.83A1-242[»]
1UTKX-ray1.53A1-242[»]
1UTLX-ray1.70M1-242[»]
1UTMX-ray1.50A1-242[»]
2STAX-ray1.80E21-242[»]
2STBX-ray1.80E21-242[»]
2TBSX-ray1.80A21-242[»]
ProteinModelPortaliP35031.
SMRiP35031.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35031.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 240Peptidase S1PROSITE-ProRule annotationAdd BLAST220

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG013304.
KOiK01312.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35031-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISLVFVLLI GAAFATEDDK IVGGYECKAY SQTHQVSLNS GYHFCGGSLV
60 70 80 90 100
NENWVVSAAH CYKSRVEVRL GEHNIKVTEG SEQFISSSRV IRHPNYSSYN
110 120 130 140 150
IDNDIMLIKL SKPATLNTYV QPVALPTSCA PAGTMCTVSG WGNTMSSTAD
160 170 180 190 200
SNKLQCLNIP ILSYSDCNNS YPGMITNAMF CAGYLEGGKD SCQGDSGGPV
210 220 230 240
VCNGELQGVV SWGYGCAEPG NPGVYAKVCI FNDWLTSTMA SY
Length:242
Mass (Da):25,958
Last modified:February 1, 1994 - v1
Checksum:i43F5642498067E5A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti33T → A in trypsins IA/IB. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70075 mRNA. Translation: CAA49680.1.
X70071 mRNA. Translation: CAA49676.1.
X70072 mRNA. Translation: CAA49677.1.
PIRiS66659. S31776.
S66660. S31775.
RefSeqiNP_001117183.1. NM_001123711.1.
NP_001119704.1. NM_001126232.1.
UniGeneiSsa.38456.
Ssa.628.

Genome annotation databases

GeneIDi100137021.
100137022.
KEGGisasa:100137021.
sasa:100137022.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70075 mRNA. Translation: CAA49680.1.
X70071 mRNA. Translation: CAA49676.1.
X70072 mRNA. Translation: CAA49677.1.
PIRiS66659. S31776.
S66660. S31775.
RefSeqiNP_001117183.1. NM_001123711.1.
NP_001119704.1. NM_001126232.1.
UniGeneiSsa.38456.
Ssa.628.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BITX-ray1.83A6-242[»]
1BZXX-ray2.10E21-242[»]
1HJ8X-ray1.00A21-242[»]
1UTJX-ray1.83A1-242[»]
1UTKX-ray1.53A1-242[»]
1UTLX-ray1.70M1-242[»]
1UTMX-ray1.50A1-242[»]
2STAX-ray1.80E21-242[»]
2STBX-ray1.80E21-242[»]
2TBSX-ray1.80A21-242[»]
ProteinModelPortaliP35031.
SMRiP35031.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-88002.

Protein family/group databases

MEROPSiS01.125.

Proteomic databases

PRIDEiP35031.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100137021.
100137022.
KEGGisasa:100137021.
sasa:100137022.

Organism-specific databases

CTDi100137021.
100137022.

Phylogenomic databases

HOVERGENiHBG013304.
KOiK01312.

Miscellaneous databases

EvolutionaryTraceiP35031.
PROiP35031.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRY1_SALSA
AccessioniPrimary (citable) accession number: P35031
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.