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P35031

- TRY1_SALSA

UniProt

P35031 - TRY1_SALSA

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Protein
Trypsin-1
Gene
N/A
Organism
Salmo salar (Atlantic salmon)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Binds 1 calcium ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei60 – 601Charge relay system
Metal bindingi72 – 721Calcium
Metal bindingi74 – 741Calcium; via carbonyl oxygen
Metal bindingi77 – 771Calcium; via carbonyl oxygen
Metal bindingi82 – 821Calcium
Active sitei104 – 1041Charge relay system
Sitei190 – 1901Required for specificity By similarity
Active sitei196 – 1961Charge relay system

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiS01.125.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-1 (EC:3.4.21.4)
Alternative name(s):
Trypsin I
OrganismiSalmo salar (Atlantic salmon)
Taxonomic identifieri8030 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeSalmo

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515 Reviewed prediction
Add
BLAST
Propeptidei16 – 205Activation peptide
PRO_0000028225
Chaini21 – 242222Trypsin-1
PRO_0000028226Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 156
Disulfide bondi45 ↔ 61
Disulfide bondi129 ↔ 229
Disulfide bondi136 ↔ 202
Disulfide bondi167 ↔ 181
Disulfide bondi192 ↔ 216

Keywords - PTMi

Disulfide bond, Zymogen

Interactioni

Protein-protein interaction databases

MINTiMINT-88002.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni29 – 346
Beta strandi35 – 5117
Beta strandi54 – 574
Helixi59 – 613
Beta strandi67 – 715
Beta strandi83 – 9210
Turni98 – 1014
Beta strandi106 – 1127
Beta strandi117 – 1193
Beta strandi135 – 1428
Beta strandi146 – 1483
Beta strandi155 – 1617
Helixi164 – 1707
Turni172 – 1743
Beta strandi179 – 1835
Turni193 – 1975
Beta strandi199 – 2024
Beta strandi205 – 2128
Beta strandi214 – 2174
Beta strandi223 – 2275
Helixi228 – 2314
Helixi232 – 2409

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BITX-ray1.83A6-242[»]
1BZXX-ray2.10E21-242[»]
1HJ8X-ray1.00A21-242[»]
1UTJX-ray1.83A1-242[»]
1UTKX-ray1.53A1-242[»]
1UTLX-ray1.70M1-242[»]
1UTMX-ray1.50A1-242[»]
2STAX-ray1.80E21-242[»]
2STBX-ray1.80E21-242[»]
2TBSX-ray1.80A21-242[»]
ProteinModelPortaliP35031.
SMRiP35031. Positions 21-242.

Miscellaneous databases

EvolutionaryTraceiP35031.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 240220Peptidase S1
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35031-1 [UniParc]FASTAAdd to Basket

« Hide

MISLVFVLLI GAAFATEDDK IVGGYECKAY SQTHQVSLNS GYHFCGGSLV    50
NENWVVSAAH CYKSRVEVRL GEHNIKVTEG SEQFISSSRV IRHPNYSSYN 100
IDNDIMLIKL SKPATLNTYV QPVALPTSCA PAGTMCTVSG WGNTMSSTAD 150
SNKLQCLNIP ILSYSDCNNS YPGMITNAMF CAGYLEGGKD SCQGDSGGPV 200
VCNGELQGVV SWGYGCAEPG NPGVYAKVCI FNDWLTSTMA SY 242
Length:242
Mass (Da):25,958
Last modified:February 1, 1994 - v1
Checksum:i43F5642498067E5A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti33 – 331T → A in trypsins IA/IB.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X70075 mRNA. Translation: CAA49680.1.
X70071 mRNA. Translation: CAA49676.1.
X70072 mRNA. Translation: CAA49677.1.
PIRiS66659. S31776.
S66660. S31775.
RefSeqiNP_001117183.1. NM_001123711.1.
NP_001119704.1. NM_001126232.1.
UniGeneiSsa.38456.
Ssa.628.

Genome annotation databases

GeneIDi100137021.
100137022.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X70075 mRNA. Translation: CAA49680.1 .
X70071 mRNA. Translation: CAA49676.1 .
X70072 mRNA. Translation: CAA49677.1 .
PIRi S66659. S31776.
S66660. S31775.
RefSeqi NP_001117183.1. NM_001123711.1.
NP_001119704.1. NM_001126232.1.
UniGenei Ssa.38456.
Ssa.628.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BIT X-ray 1.83 A 6-242 [» ]
1BZX X-ray 2.10 E 21-242 [» ]
1HJ8 X-ray 1.00 A 21-242 [» ]
1UTJ X-ray 1.83 A 1-242 [» ]
1UTK X-ray 1.53 A 1-242 [» ]
1UTL X-ray 1.70 M 1-242 [» ]
1UTM X-ray 1.50 A 1-242 [» ]
2STA X-ray 1.80 E 21-242 [» ]
2STB X-ray 1.80 E 21-242 [» ]
2TBS X-ray 1.80 A 21-242 [» ]
ProteinModelPortali P35031.
SMRi P35031. Positions 21-242.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-88002.

Protein family/group databases

MEROPSi S01.125.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100137021.
100137022.

Organism-specific databases

CTDi 100137021.
100137022.

Phylogenomic databases

HOVERGENi HBG013304.

Miscellaneous databases

EvolutionaryTracei P35031.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and characterization of anionic and cationic variants of trypsin from Atlantic salmon."
    Male R., Lorens J.B., Smals A.O., Torrissen K.R.
    Eur. J. Biochem. 232:677-685(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pancreas.
  2. "Structure determination and refinement of benzamidine-inhibited trypsin from the North Atlantic salmon (Salmo salar) at 1.82-A resolution."
    Smalas A.O., Hordvik A.
    Acta Crystallogr. D 49:318-330(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS).
  3. "Cold adaption of enzymes: structural comparison between salmon and bovine trypsins."
    Smalas A.O., Heimstad E.S., Hordvik A., Willassen N.P., Male R.
    Proteins 20:149-166(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS).

Entry informationi

Entry nameiTRY1_SALSA
AccessioniPrimary (citable) accession number: P35031
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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