UniProtKB - P35030 (TRY3_HUMAN)
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Protein
Trypsin-3
Gene
PRSS3
Organism
Homo sapiens (Human)
Status
Functioni
Digestive protease specialized for the degradation of trypsin inhibitors. In the ileum, may be involved in defensin processing, including DEFA5.2 Publications
Catalytic activityi
Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactori
Ca2+Note: Binds 1 Ca2+ ion per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 120 | Charge relay system | 1 | |
Metal bindingi | 132 | Calcium | 1 | |
Metal bindingi | 134 | Calcium; via carbonyl oxygen | 1 | |
Metal bindingi | 137 | Calcium; via carbonyl oxygen | 1 | |
Metal bindingi | 142 | Calcium | 1 | |
Active sitei | 164 | Charge relay system | 1 | |
Sitei | 251 | Required for specificityBy similarity | 1 | |
Active sitei | 257 | Charge relay system | 1 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- serine-type endopeptidase activity Source: UniProtKB
- serine-type peptidase activity Source: UniProtKB
GO - Biological processi
- antimicrobial humoral response Source: Reactome
- cobalamin metabolic process Source: Reactome
- digestion Source: UniProtKB
- endothelial cell migration Source: UniProtKB
- neutrophil degranulation Source: Reactome
- proteolysis Source: UniProtKB
- zymogen activation Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Digestion |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-HSA-1462054 Alpha-defensins R-HSA-196741 Cobalamin (Cbl, vitamin B12) transport and metabolism R-HSA-6798695 Neutrophil degranulation R-HSA-6803157 Antimicrobial peptides |
Protein family/group databases
MEROPSi | S01.174 |
Names & Taxonomyi
Protein namesi | Recommended name: Trypsin-3 (EC:3.4.21.4)Alternative name(s): Brain trypsinogen Mesotrypsinogen Serine protease 3 Serine protease 4 Trypsin III Trypsin IV |
Gene namesi | Name:PRSS3 Synonyms:PRSS4, TRY3, TRY4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000010438.16 |
HGNCi | HGNC:9486 PRSS3 |
MIMi | 613578 gene |
neXtProti | NX_P35030 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 5646 |
OpenTargetsi | ENSG00000010438 |
PharmGKBi | PA33838 |
Chemistry databases
ChEMBLi | CHEMBL4551 |
DrugBanki | DB02585 4-(Hydroxymethyl)Benzamidine DB02541 4-Hydroxybutan-1-Aminium DB03127 Benzamidine DB03637 Guanidine-3-Propanol |
GuidetoPHARMACOLOGYi | 2399 |
Polymorphism and mutation databases
BioMutai | PRSS3 |
DMDMi | 209572698 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000028201 | ? – 80 | Activation peptide | ||
Signal peptidei | 1 – ? | Sequence analysis | ||
ChainiPRO_0000028202 | 81 – 304 | Trypsin-3Add BLAST | 224 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 87 ↔ 217 | |||
Disulfide bondi | 105 ↔ 121 | |||
Disulfide bondi | 196 ↔ 263 | |||
Modified residuei | 211 | SulfotyrosineBy similarity | 1 | |
Disulfide bondi | 228 ↔ 242 | |||
Disulfide bondi | 253 ↔ 277 |
Keywords - PTMi
Disulfide bond, Sulfation, ZymogenProteomic databases
EPDi | P35030 |
MaxQBi | P35030 |
PaxDbi | P35030 |
PeptideAtlasi | P35030 |
PRIDEi | P35030 |
PTM databases
iPTMneti | P35030 |
PhosphoSitePlusi | P35030 |
Expressioni
Tissue specificityi
Expressed in pancreas and brain. Also expressed in Paneth cells, at the base of small intestinal crypts.1 Publication
Gene expression databases
Bgeei | ENSG00000010438 |
CleanExi | HS_PRSS3 |
ExpressionAtlasi | P35030 baseline and differential |
Genevisiblei | P35030 HS |
Organism-specific databases
HPAi | HPA062452 HPA063471 |
Interactioni
Protein-protein interaction databases
BioGridi | 11162810 interactors. |
IntActi | P35030 8 interactors. |
MINTi | P35030 |
STRINGi | 9606.ENSP00000354280 |
Chemistry databases
BindingDBi | P35030 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Beta strandi | 89 – 91 | Combined sources | 3 | |
Beta strandi | 95 – 111 | Combined sources | 17 | |
Beta strandi | 114 – 117 | Combined sources | 4 | |
Helixi | 119 – 121 | Combined sources | 3 | |
Beta strandi | 127 – 131 | Combined sources | 5 | |
Beta strandi | 133 – 137 | Combined sources | 5 | |
Beta strandi | 143 – 152 | Combined sources | 10 | |
Turni | 158 – 160 | Combined sources | 3 | |
Beta strandi | 166 – 172 | Combined sources | 7 | |
Beta strandi | 177 – 180 | Combined sources | 4 | |
Beta strandi | 195 – 201 | Combined sources | 7 | |
Beta strandi | 206 – 208 | Combined sources | 3 | |
Beta strandi | 216 – 222 | Combined sources | 7 | |
Helixi | 225 – 231 | Combined sources | 7 | |
Turni | 233 – 235 | Combined sources | 3 | |
Beta strandi | 240 – 244 | Combined sources | 5 | |
Beta strandi | 246 – 249 | Combined sources | 4 | |
Turni | 254 – 258 | Combined sources | 5 | |
Beta strandi | 260 – 263 | Combined sources | 4 | |
Beta strandi | 266 – 273 | Combined sources | 8 | |
Beta strandi | 275 – 278 | Combined sources | 4 | |
Beta strandi | 284 – 288 | Combined sources | 5 | |
Helixi | 289 – 292 | Combined sources | 4 | |
Helixi | 293 – 302 | Combined sources | 10 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1H4W | X-ray | 1.70 | A | 81-304 | [»] | |
2R9P | X-ray | 1.40 | A/B/C/D | 81-304 | [»] | |
3L33 | X-ray | 2.48 | A/B/C/D | 81-304 | [»] | |
3L3T | X-ray | 2.38 | A/B/C/D | 81-304 | [»] | |
3P92 | X-ray | 1.60 | A | 81-304 | [»] | |
3P95 | X-ray | 1.30 | A | 81-304 | [»] | |
4DG4 | X-ray | 1.40 | A/B/D/G | 81-304 | [»] | |
4U30 | X-ray | 2.50 | A/B/C/D | 81-304 | [»] | |
4U32 | X-ray | 1.65 | A | 81-304 | [»] | |
5C67 | X-ray | 1.83 | A/B | 81-304 | [»] | |
5JBT | X-ray | 1.40 | A | 81-304 | [»] | |
5TP0 | X-ray | 1.25 | A | 81-304 | [»] | |
ProteinModelPortali | P35030 | |||||
SMRi | P35030 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P35030 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 81 – 301 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 221 |
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627 Eukaryota COG5640 LUCA |
GeneTreei | ENSGT00760000118862 |
HOGENOMi | HOG000251820 |
HOVERGENi | HBG013304 |
InParanoidi | P35030 |
KOi | K01312 |
OMAi | NEQFINE |
OrthoDBi | EOG091G0DF7 |
PhylomeDBi | P35030 |
TreeFami | TF331065 |
Family and domain databases
CDDi | cd00190 Tryp_SPc, 1 hit |
InterProi | View protein in InterPro IPR009003 Peptidase_S1_PA IPR001314 Peptidase_S1A IPR001254 Trypsin_dom IPR018114 TRYPSIN_HIS |
Pfami | View protein in Pfam PF00089 Trypsin, 1 hit |
PRINTSi | PR00722 CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020 Tryp_SPc, 1 hit |
SUPFAMi | SSF50494 SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240 TRYPSIN_DOM, 1 hit PS00134 TRYPSIN_HIS, 1 hit |
s (5)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basket
Isoform 1 (identifier: P35030-1) [UniParc]FASTAAdd to basket
Also known as: A
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MCGPDDRCPA RWPGPGRAVK CGKGLAAARP GRVERGGAQR GGAGLELHPL
60 70 80 90 100
LGGRTWRAAR DADGCEALGT VAVPFDDDDK IVGGYTCEEN SLPYQVSLNS
110 120 130 140 150
GSHFCGGSLI SEQWVVSAAH CYKTRIQVRL GEHNIKVLEG NEQFINAAKI
160 170 180 190 200
IRHPKYNRDT LDNDIMLIKL SSPAVINARV STISLPTTPP AAGTECLISG
210 220 230 240 250
WGNTLSFGAD YPDELKCLDA PVLTQAECKA SYPGKITNSM FCVGFLEGGK
260 270 280 290 300
DSCQRDSGGP VVCNGQLQGV VSWGHGCAWK NRPGVYTKVY NYVDWIKDTI
AANS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 89 | Missing in CAA50484 (PubMed:8294000).Curated | 1 | |
Sequence conflicti | 224 – 225 | TQ → RE in CAA33527 (PubMed:2326201).Curated | 2 | |
Sequence conflicti | 253 – 254 | CQ → WK in CAA33527 (PubMed:2326201).Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_067459 | 174 | A → V. Corresponds to variant dbSNP:rs11547028Ensembl. | 1 | |
Natural variantiVAR_046794 | 188 | T → A7 PublicationsCorresponds to variant dbSNP:rs855581Ensembl. | 1 | |
Natural variantiVAR_059788 | 224 | T → S. Corresponds to variant dbSNP:rs1063273Ensembl. | 1 | |
Natural variantiVAR_046795 | 232 | Y → C1 PublicationCorresponds to variant dbSNP:rs1048379Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_005409 | 1 – 71 | MCGPD…ALGTV → MHMRETSGFTLKKGRSAPLV FHPPDALI in isoform 4. CuratedAdd BLAST | 71 | |
Alternative sequenceiVSP_005410 | 1 – 70 | MCGPD…EALGT → MNPFLILAFVGAA in isoform 3. 3 PublicationsAdd BLAST | 70 | |
Alternative sequenceiVSP_053779 | 1 – 70 | MCGPD…EALGT → MGPAGE in isoform 5. 1 PublicationAdd BLAST | 70 | |
Alternative sequenceiVSP_042074 | 1 – 45 | MCGPD…GGAGL → M in isoform 2. 1 PublicationAdd BLAST | 45 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72781 mRNA Translation: CAB58178.1 X71345 mRNA Translation: CAA50484.1 X15505 mRNA Translation: CAA33527.1 D45417 mRNA Translation: BAA08257.1 AF029308 Genomic DNA Translation: AAC13322.1 AB298285 mRNA Translation: BAF80324.1 AB298286 mRNA Translation: BAF80325.1 AL139113 Genomic DNA No translation available. AL356489 Genomic DNA No translation available. AL358573 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58482.1 CH471071 Genomic DNA Translation: EAW58484.1 BC069476 mRNA Translation: AAH69476.1 BC069494 mRNA Translation: AAH69494.1 |
CCDSi | CCDS47958.1 [P35030-1] CCDS56570.1 [P35030-5] CCDS56571.1 [P35030-4] CCDS6545.1 [P35030-3] |
PIRi | S12764 S33496 |
RefSeqi | NP_001184026.2, NM_001197097.2 NP_001184027.1, NM_001197098.1 NP_002762.2, NM_002771.3 NP_031369.2, NM_007343.3 |
UniGenei | Hs.654513 |
Genome annotation databases
Ensembli | ENST00000342836; ENSP00000340889; ENSG00000010438 [P35030-4] ENST00000361005; ENSP00000354280; ENSG00000010438 [P35030-1] ENST00000379405; ENSP00000368715; ENSG00000010438 [P35030-3] ENST00000429677; ENSP00000401828; ENSG00000010438 [P35030-5] |
GeneIDi | 5646 |
KEGGi | hsa:5646 |
UCSCi | uc003zti.5 human [P35030-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Entry informationi
Entry namei | TRY3_HUMAN | |
Accessioni | P35030Primary (citable) accession number: P35030 Secondary accession number(s): A8CED1 Q9UQV3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | October 14, 2008 | |
Last modified: | February 28, 2018 | |
This is version 180 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |