P35029 (SYA_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Saci_1455

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	907 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 907	907	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_0000075276
Sites
	Metal binding	606	1	Zinc By similarity
	Metal binding	610	1	Zinc By similarity
	Metal binding	710	1	Zinc By similarity
	Metal binding	714	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35029 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: 86807784D2351F3E	FASTA	907	103,899
	10 20 30 40 50 60 MVKANENEYR LNFFLSRGYE RKICRSCSTP FWTLDKSKEN CSDVPCTDYY FFDIKIKSPP 70 80 90 100 110 120 LTVRESREKF LRFFEKRGHT IVPPKPVVAK WRDDLYLTIA SIVDFQPFVT SGLAKPPANP 130 140 150 160 170 180 LVVSQPCIRL EDVDNVGITF GRHLTTFEMA AHHAFNYPDK QIYWKDETVA FAKEFFTEEL 190 200 210 220 230 240 GIPEDQLNFK ESWWEGGGNA GPCFEVTVGG LELATLVFMQ YEIRGQDYVP LKLKIVDTGY 250 260 270 280 290 300 GVERIAWFTQ RTPTAFHAIY GNLVSSFYKK IGVGEVNNEL LKAAAIYAGR IDPDIKETIT 310 320 330 340 350 360 AHREHLAKQL GLDLKYVNEE LTRAARVFQV LDHTKTIALM LADGLVPSNS GEGYLGRLLI 370 380 390 400 410 420 RRALRVLRLL GSDVKLHELI KDQIDYWKED FPQMLKNKDY IVDAVINEEE KYNDTISKIP 430 440 450 460 470 480 SILSTLSKKS KVDLDELINI YDSNGISPDL ILDEARKRNI NINVEIPHNF YSIVAKKHQN 490 500 510 520 530 540 ALVRENRKDK IPKEVIDEIN NKKIEPTVPL YYKDQYLRTF NAKVLLNYKN FLVLDQTTFY 550 560 570 580 590 600 PEGGGQIGDT GIIRSIEGNK QAKVIDTQKY KGVIVHILDK DSPFIQGEQV YGEIDWQRRY 610 620 630 640 650 660 RIMKHHTVTH VILSATRRVL GEHAWQAGAE KTEYKGRLDV THYKLPTEEE IRKIEDFANY 670 680 690 700 710 720 IINDRRKVRP LYIERTEAEM KYGVSIYAGG IPEGSEIRLI EIENWDIEGC GGTHLINTGE 730 740 750 760 770 780 IGGVKIVNVE KLQDGVIRLE YVAGDMVSNY ARQQDEKLNE ISKLLNSPVS QINVRLKKHL 790 800 810 820 830 840 EEYENLQNLL DKYRKIVLDR IQEIAERISV NGITIYILRD FIDEQLIKEV MRKITSNNQN 850 860 870 880 890 900 IVISIRGKDT KNVEIATSKD IKVDKIVDEL RKIGGRGGGK GTYGSVSITV EEEKIIDTIR SAITNGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed: 15995215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
[2]	"Structure and transcription of the L11-L1-L10-L12 ribosomal protein gene operon from the extreme thermophilic archaeon Sulfolobus acidocaldarius." Ramirez C., Shimmin L.C., Leggatt P., Matheson A.T. J. Mol. Biol. 244:242-249(1994) [PubMed: 7966335] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-182.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000077 Genomic DNA. Translation: AAY80776.1. X59038 Genomic DNA. Translation: CAA41766.1.
PIR	S53652.
RefSeq	YP_256069.1. NC_007181.1.
3D structure databases
ProteinModelPortal	P35029.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3473494.
GenomeReviews	Gene locus Saci_1455 in contig CP000077_GR.
KEGG	sai:Saci_1455.
NMPDR	fig|330779.3.peg.2148.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG392147.
OMA	GESKTDQ.
PhylomeDB	P35029.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	SACI330779:SACI_1455-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_SULAC

Accession

Primary (citable) accession number: P35029
Secondary accession number(s): Q4J8V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	August 30, 2005
Last modified:	January 25, 2012
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program