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Protein

Elongation factor 1-alpha

Gene

tuf

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 208GTPUniRule annotation
Nucleotide bindingi90 – 945GTPUniRule annotation
Nucleotide bindingi152 – 1554GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-206-MONOMER.
BRENDAi3.6.5.3. 6163.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alphaUniRule annotation
Short name:
EF-1-alphaUniRule annotation
Alternative name(s):
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufUniRule annotation
Synonyms:tef1
Ordered Locus Names:SSO0216
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435Elongation factor 1-alphaPRO_0000090994Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi273057.SSO0216.

Structurei

Secondary structure

1
435
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 149Combined sources
Helixi19 – 3012Combined sources
Helixi35 – 4814Combined sources
Helixi52 – 6413Combined sources
Beta strandi78 – 803Combined sources
Beta strandi85 – 884Combined sources
Beta strandi92 – 943Combined sources
Helixi97 – 1026Combined sources
Beta strandi104 – 1063Combined sources
Beta strandi109 – 1168Combined sources
Helixi121 – 1266Combined sources
Helixi131 – 14111Combined sources
Beta strandi148 – 1525Combined sources
Helixi154 – 1563Combined sources
Beta strandi157 – 1593Combined sources
Helixi163 – 17917Combined sources
Beta strandi188 – 1914Combined sources
Turni194 – 1974Combined sources
Helixi215 – 2195Combined sources
Helixi228 – 2303Combined sources
Beta strandi234 – 24310Combined sources
Turni244 – 2463Combined sources
Beta strandi247 – 2537Combined sources
Beta strandi264 – 2685Combined sources
Turni269 – 2713Combined sources
Beta strandi272 – 28110Combined sources
Beta strandi284 – 2896Combined sources
Beta strandi294 – 3029Combined sources
Helixi304 – 3063Combined sources
Beta strandi312 – 3143Combined sources
Beta strandi322 – 33211Combined sources
Beta strandi346 – 3494Combined sources
Beta strandi352 – 36514Combined sources
Turni367 – 3693Combined sources
Beta strandi371 – 3766Combined sources
Beta strandi378 – 3803Combined sources
Beta strandi385 – 39410Combined sources
Turni401 – 4033Combined sources
Helixi405 – 4073Combined sources
Beta strandi409 – 4146Combined sources
Beta strandi417 – 42812Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JNYX-ray1.80A/B1-435[»]
1SKQX-ray1.80A/B1-435[»]
ProteinModelPortaliP35021.
SMRiP35021. Positions 4-430.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35021.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 227224tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni13 – 208G1By similarity
Regioni69 – 735G2By similarity
Regioni90 – 934G3By similarity
Regioni152 – 1554G4By similarity
Regioni193 – 1953G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01561. Archaea.
COG5256. LUCA.
HOGENOMiHOG000229291.
InParanoidiP35021.
KOiK03231.
OMAiPASTIFH.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35021-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKPHLNLI VIGHIDHGKS TLVGRLLMDR GFIDEKTVKE AEEAAKKLGK
60 70 80 90 100
ESEKFAFLLD RLKEERERGV TINLTFMRFE TKKYFFTIID APGHRDFVKN
110 120 130 140 150
MITGASQADA AILVVSAKKG EYEAGMSVEG QTREHIILAK TMGLDQLIVA
160 170 180 190 200
VNKMDLTEPP YDEKRYKEIV DQVSKFMRSY GFNTNKVRFV PVVAPAGDNI
210 220 230 240 250
THRSENMKWY NGPTLEEYLD QLELPPKPVD KPLRIPIQDV YSISGVGTVP
260 270 280 290 300
VGRVESGVLK VGDKIVFMPA GKVGEVRSIE THHTKMDKAE PGDNIGFNVR
310 320 330 340 350
GVEKKDIKRG DVVGHPNNPP TVADEFTARI IVVWHPTALA NGYTPVIHVH
360 370 380 390 400
TASVACRVSE LVSKLDPRTG QEAEKNPQFL KQGDVAIVKF KPIKPLCVEK
410 420 430
YNEFPPLGRF AMRDMGKTVG VGIIVDVKPA KVEIK
Length:435
Mass (Da):48,489
Last modified:June 1, 2001 - v3
Checksum:i222B540A31768A59
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141H → Q (PubMed:8369039).Curated
Sequence conflicti240 – 2401V → R (PubMed:8369039).Curated
Sequence conflicti240 – 2401V → R (PubMed:8148382).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti196 – 1961A → S in strain: MT-3 and MT-4.
Natural varianti203 – 2031R → K in strain: MT-3 and MT-4.
Natural varianti347 – 3471I → L in strain: MT-3 and MT-4.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70701 Genomic DNA. Translation: CAA50033.1.
X76767 Genomic DNA. Translation: CAA54162.1.
AJ312397 Genomic DNA. Translation: CAC42886.1.
AE006641 Genomic DNA. Translation: AAK40559.1.
PIRiH90162.
S43507.
RefSeqiWP_010922916.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK40559; AAK40559; SSO0216.
GeneIDi1455369.
KEGGisso:SSO0216.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70701 Genomic DNA. Translation: CAA50033.1.
X76767 Genomic DNA. Translation: CAA54162.1.
AJ312397 Genomic DNA. Translation: CAC42886.1.
AE006641 Genomic DNA. Translation: AAK40559.1.
PIRiH90162.
S43507.
RefSeqiWP_010922916.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JNYX-ray1.80A/B1-435[»]
1SKQX-ray1.80A/B1-435[»]
ProteinModelPortaliP35021.
SMRiP35021. Positions 4-430.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO0216.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK40559; AAK40559; SSO0216.
GeneIDi1455369.
KEGGisso:SSO0216.

Phylogenomic databases

eggNOGiarCOG01561. Archaea.
COG5256. LUCA.
HOGENOMiHOG000229291.
InParanoidiP35021.
KOiK03231.
OMAiPASTIFH.

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-206-MONOMER.
BRENDAi3.6.5.3. 6163.

Miscellaneous databases

EvolutionaryTraceiP35021.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of the elongation factor 1 alpha in Sulfolobus solfataricus."
    Arcari P., Gallo M., Ianniciello G., Dello Russo A., Bocchini V.
    Nucleic Acids Res. 21:1666-1666(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 5833 / MT-4.
  2. "The nucleotide sequence of the gene coding for the elongation factor 1 alpha in Sulfolobus solfataricus. Homology of the product with related proteins."
    Arcari P., Gallo M., Ianniciello G., Dello Russo A., Bocchini V.
    Biochim. Biophys. Acta 1217:333-337(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 5833 / MT-4.
  3. "The nucleotide sequence of the gene encoding the elongation factor 1 alpha from the archaeon Sulfolobus solfataricus isolate MT3."
    Arcari P., Masullo M., Bocchini V.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MT-3.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Entry informationi

Entry nameiEF1A_SULSO
AccessioniPrimary (citable) accession number: P35021
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 2001
Last modified: February 17, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.