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Protein

Elongation factor 1-alpha

Gene

tuf

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 20GTPUniRule annotation8
Nucleotide bindingi90 – 94GTPUniRule annotation5
Nucleotide bindingi152 – 155GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.5.3. 6163.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alphaUniRule annotation
Short name:
EF-1-alphaUniRule annotation
Alternative name(s):
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufUniRule annotation
Synonyms:tef1
Ordered Locus Names:SSO0216
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000909941 – 435Elongation factor 1-alphaAdd BLAST435

Interactioni

Protein-protein interaction databases

STRINGi273057.SSO0216.

Structurei

Secondary structure

1435
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 14Combined sources9
Helixi19 – 30Combined sources12
Helixi35 – 48Combined sources14
Helixi52 – 64Combined sources13
Beta strandi78 – 80Combined sources3
Beta strandi85 – 88Combined sources4
Beta strandi92 – 94Combined sources3
Helixi97 – 102Combined sources6
Beta strandi104 – 106Combined sources3
Beta strandi109 – 116Combined sources8
Helixi121 – 126Combined sources6
Helixi131 – 141Combined sources11
Beta strandi148 – 152Combined sources5
Helixi154 – 156Combined sources3
Beta strandi157 – 159Combined sources3
Helixi163 – 179Combined sources17
Beta strandi188 – 191Combined sources4
Turni194 – 197Combined sources4
Helixi215 – 219Combined sources5
Helixi228 – 230Combined sources3
Beta strandi234 – 243Combined sources10
Turni244 – 246Combined sources3
Beta strandi247 – 253Combined sources7
Beta strandi264 – 268Combined sources5
Turni269 – 271Combined sources3
Beta strandi272 – 281Combined sources10
Beta strandi284 – 289Combined sources6
Beta strandi294 – 302Combined sources9
Helixi304 – 306Combined sources3
Beta strandi312 – 314Combined sources3
Beta strandi322 – 332Combined sources11
Beta strandi346 – 349Combined sources4
Beta strandi352 – 365Combined sources14
Turni367 – 369Combined sources3
Beta strandi371 – 376Combined sources6
Beta strandi378 – 380Combined sources3
Beta strandi385 – 394Combined sources10
Turni401 – 403Combined sources3
Helixi405 – 407Combined sources3
Beta strandi409 – 414Combined sources6
Beta strandi417 – 428Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JNYX-ray1.80A/B1-435[»]
1SKQX-ray1.80A/B1-435[»]
ProteinModelPortaliP35021.
SMRiP35021.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35021.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 227tr-type GAdd BLAST224

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 20G1By similarity8
Regioni69 – 73G2By similarity5
Regioni90 – 93G3By similarity4
Regioni152 – 155G4By similarity4
Regioni193 – 195G5By similarity3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01561. Archaea.
COG5256. LUCA.
HOGENOMiHOG000229291.
InParanoidiP35021.
KOiK03231.
OMAiSIDAQPQ.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35021-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKPHLNLI VIGHIDHGKS TLVGRLLMDR GFIDEKTVKE AEEAAKKLGK
60 70 80 90 100
ESEKFAFLLD RLKEERERGV TINLTFMRFE TKKYFFTIID APGHRDFVKN
110 120 130 140 150
MITGASQADA AILVVSAKKG EYEAGMSVEG QTREHIILAK TMGLDQLIVA
160 170 180 190 200
VNKMDLTEPP YDEKRYKEIV DQVSKFMRSY GFNTNKVRFV PVVAPAGDNI
210 220 230 240 250
THRSENMKWY NGPTLEEYLD QLELPPKPVD KPLRIPIQDV YSISGVGTVP
260 270 280 290 300
VGRVESGVLK VGDKIVFMPA GKVGEVRSIE THHTKMDKAE PGDNIGFNVR
310 320 330 340 350
GVEKKDIKRG DVVGHPNNPP TVADEFTARI IVVWHPTALA NGYTPVIHVH
360 370 380 390 400
TASVACRVSE LVSKLDPRTG QEAEKNPQFL KQGDVAIVKF KPIKPLCVEK
410 420 430
YNEFPPLGRF AMRDMGKTVG VGIIVDVKPA KVEIK
Length:435
Mass (Da):48,489
Last modified:June 1, 2001 - v3
Checksum:i222B540A31768A59
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14H → Q (PubMed:8369039).Curated1
Sequence conflicti240V → R (PubMed:8369039).Curated1
Sequence conflicti240V → R (PubMed:8148382).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti196A → S in strain: MT-3 and MT-4. 1
Natural varianti203R → K in strain: MT-3 and MT-4. 1
Natural varianti347I → L in strain: MT-3 and MT-4. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70701 Genomic DNA. Translation: CAA50033.1.
X76767 Genomic DNA. Translation: CAA54162.1.
AJ312397 Genomic DNA. Translation: CAC42886.1.
AE006641 Genomic DNA. Translation: AAK40559.1.
PIRiH90162.
S43507.
RefSeqiWP_010922916.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK40559; AAK40559; SSO0216.
GeneIDi27426513.
KEGGisso:SSO0216.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70701 Genomic DNA. Translation: CAA50033.1.
X76767 Genomic DNA. Translation: CAA54162.1.
AJ312397 Genomic DNA. Translation: CAC42886.1.
AE006641 Genomic DNA. Translation: AAK40559.1.
PIRiH90162.
S43507.
RefSeqiWP_010922916.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JNYX-ray1.80A/B1-435[»]
1SKQX-ray1.80A/B1-435[»]
ProteinModelPortaliP35021.
SMRiP35021.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO0216.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK40559; AAK40559; SSO0216.
GeneIDi27426513.
KEGGisso:SSO0216.

Phylogenomic databases

eggNOGiarCOG01561. Archaea.
COG5256. LUCA.
HOGENOMiHOG000229291.
InParanoidiP35021.
KOiK03231.
OMAiSIDAQPQ.

Enzyme and pathway databases

BRENDAi3.6.5.3. 6163.

Miscellaneous databases

EvolutionaryTraceiP35021.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF1A_SULSO
AccessioniPrimary (citable) accession number: P35021
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.