Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P35005

- TRYE_DROME

UniProt

P35005 - TRYE_DROME

Protein

Trypsin epsilon

Gene

epsilonTry

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei71 – 711Charge relay systemBy similarity
    Active sitei116 – 1161Charge relay systemBy similarity
    Sitei204 – 2041Required for specificityBy similarity
    Active sitei210 – 2101Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: FlyBase

    GO - Biological processi

    1. proteolysis Source: FlyBase

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.A82.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin epsilon (EC:3.4.21.4)
    Gene namesi
    Name:epsilonTry
    Synonyms:TRY-EPSILON
    ORF Names:CG18681
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0010425. epsilonTry.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222CuratedAdd
    BLAST
    Propeptidei23 – 308Activation peptidePRO_0000028273
    Chaini31 – 256226Trypsin epsilonPRO_0000028274Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi56 ↔ 72PROSITE-ProRule annotation
    Disulfide bondi180 ↔ 197PROSITE-ProRule annotation
    Disulfide bondi206 ↔ 230PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Proteomic databases

    PaxDbiP35005.
    PRIDEiP35005.

    Expressioni

    Gene expression databases

    BgeeiP35005.

    Interactioni

    Protein-protein interaction databases

    BioGridi71965. 2 interactions.
    DIPiDIP-21329N.
    IntActiP35005. 1 interaction.
    MINTiMINT-907623.

    Structurei

    3D structure databases

    ProteinModelPortaliP35005.
    SMRiP35005. Positions 31-253.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 254224Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00620000088101.
    InParanoidiP35005.
    KOiK01312.
    OMAiYSHDIVI.
    OrthoDBiEOG7MKW6Q.
    PhylomeDBiP35005.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35005-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKFAVLLSV LACALAGTIP DGLLPQLDGR IVGGYETSID AHPYQVSLQR    50
    YGSHFCGGSI YSHDIVITAA HCLQSIEAKD LKIRVGSTYW RSGGSVHSVR 100
    SFRNHEGYNS RTMVNDIAII RIESDLSFRS SIREIRIADS NPREGATAVV 150
    SGWGTTESGG STIPDHLLAV DLEIIDVSRC RSDEFGYGKK IKDTMLCAYA 200
    PHKDACQGDS GGPLVSGDRL VGVVSWGYGC GDVRYPGVYA DVAHFHEWIE 250
    RTAEEV 256
    Length:256
    Mass (Da):27,810
    Last modified:February 1, 1994 - v1
    Checksum:iCB8B276430F8616D
    GO

    Sequence cautioni

    The sequence AAT27275.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96372 Genomic DNA. Translation: AAA28981.1.
    U04853 Genomic DNA. Translation: AAA17452.1.
    AE013599 Genomic DNA. Translation: AAF58660.1.
    BT014651 mRNA. Translation: AAT27275.1. Different initiation.
    RefSeqiNP_525112.1. NM_080373.3.
    UniGeneiDm.1624.

    Genome annotation databases

    EnsemblMetazoaiFBtr0088160; FBpp0087256; FBgn0010425.
    GeneIDi49080.
    KEGGidme:Dmel_CG18681.
    UCSCiCG18681-RA. d. melanogaster.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96372 Genomic DNA. Translation: AAA28981.1 .
    U04853 Genomic DNA. Translation: AAA17452.1 .
    AE013599 Genomic DNA. Translation: AAF58660.1 .
    BT014651 mRNA. Translation: AAT27275.1 . Different initiation.
    RefSeqi NP_525112.1. NM_080373.3.
    UniGenei Dm.1624.

    3D structure databases

    ProteinModelPortali P35005.
    SMRi P35005. Positions 31-253.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 71965. 2 interactions.
    DIPi DIP-21329N.
    IntActi P35005. 1 interaction.
    MINTi MINT-907623.

    Protein family/group databases

    MEROPSi S01.A82.

    Proteomic databases

    PaxDbi P35005.
    PRIDEi P35005.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0088160 ; FBpp0087256 ; FBgn0010425 .
    GeneIDi 49080.
    KEGGi dme:Dmel_CG18681.
    UCSCi CG18681-RA. d. melanogaster.

    Organism-specific databases

    CTDi 49080.
    FlyBasei FBgn0010425. epsilonTry.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00620000088101.
    InParanoidi P35005.
    KOi K01312.
    OMAi YSHDIVI.
    OrthoDBi EOG7MKW6Q.
    PhylomeDBi P35005.

    Miscellaneous databases

    GenomeRNAii 49080.
    NextBioi 839635.

    Gene expression databases

    Bgeei P35005.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Concerted evolution within a trypsin gene cluster in Drosophila."
      Wang S., Magoulas C., Hickey D.A.
      Mol. Biol. Evol. 16:1117-1124(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Oregon-R.
    2. Wang S., Magoulas C., Hickey D.A.
      Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Oregon-R.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    5. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
      Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.

    Entry informationi

    Entry nameiTRYE_DROME
    AccessioniPrimary (citable) accession number: P35005
    Secondary accession number(s): Q6IDF4, Q9V5Y1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3