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P35005

- TRYE_DROME

UniProt

P35005 - TRYE_DROME

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Protein

Trypsin epsilon

Gene

epsilonTry

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711Charge relay systemBy similarity
Active sitei116 – 1161Charge relay systemBy similarity
Sitei204 – 2041Required for specificityBy similarity
Active sitei210 – 2101Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: FlyBase

GO - Biological processi

  1. proteolysis Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.A82.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin epsilon (EC:3.4.21.4)
Gene namesi
Name:epsilonTry
Synonyms:TRY-EPSILON
ORF Names:CG18681
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0010425. epsilonTry.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222CuratedAdd
BLAST
Propeptidei23 – 308Activation peptidePRO_0000028273
Chaini31 – 256226Trypsin epsilonPRO_0000028274Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi56 ↔ 72PROSITE-ProRule annotation
Disulfide bondi180 ↔ 197PROSITE-ProRule annotation
Disulfide bondi206 ↔ 230PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP35005.
PRIDEiP35005.

Expressioni

Gene expression databases

BgeeiP35005.

Interactioni

Protein-protein interaction databases

BioGridi71965. 2 interactions.
DIPiDIP-21329N.
IntActiP35005. 1 interaction.
MINTiMINT-907623.

Structurei

3D structure databases

ProteinModelPortaliP35005.
SMRiP35005. Positions 31-253.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 254224Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00620000088101.
InParanoidiP35005.
KOiK01312.
OMAiYSHDIVI.
OrthoDBiEOG7MKW6Q.
PhylomeDBiP35005.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35005-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKFAVLLSV LACALAGTIP DGLLPQLDGR IVGGYETSID AHPYQVSLQR
60 70 80 90 100
YGSHFCGGSI YSHDIVITAA HCLQSIEAKD LKIRVGSTYW RSGGSVHSVR
110 120 130 140 150
SFRNHEGYNS RTMVNDIAII RIESDLSFRS SIREIRIADS NPREGATAVV
160 170 180 190 200
SGWGTTESGG STIPDHLLAV DLEIIDVSRC RSDEFGYGKK IKDTMLCAYA
210 220 230 240 250
PHKDACQGDS GGPLVSGDRL VGVVSWGYGC GDVRYPGVYA DVAHFHEWIE

RTAEEV
Length:256
Mass (Da):27,810
Last modified:February 1, 1994 - v1
Checksum:iCB8B276430F8616D
GO

Sequence cautioni

The sequence AAT27275.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96372 Genomic DNA. Translation: AAA28981.1.
U04853 Genomic DNA. Translation: AAA17452.1.
AE013599 Genomic DNA. Translation: AAF58660.1.
BT014651 mRNA. Translation: AAT27275.1. Different initiation.
RefSeqiNP_525112.1. NM_080373.3.
UniGeneiDm.1624.

Genome annotation databases

EnsemblMetazoaiFBtr0088160; FBpp0087256; FBgn0010425.
GeneIDi49080.
KEGGidme:Dmel_CG18681.
UCSCiCG18681-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96372 Genomic DNA. Translation: AAA28981.1 .
U04853 Genomic DNA. Translation: AAA17452.1 .
AE013599 Genomic DNA. Translation: AAF58660.1 .
BT014651 mRNA. Translation: AAT27275.1 . Different initiation.
RefSeqi NP_525112.1. NM_080373.3.
UniGenei Dm.1624.

3D structure databases

ProteinModelPortali P35005.
SMRi P35005. Positions 31-253.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 71965. 2 interactions.
DIPi DIP-21329N.
IntActi P35005. 1 interaction.
MINTi MINT-907623.

Protein family/group databases

MEROPSi S01.A82.

Proteomic databases

PaxDbi P35005.
PRIDEi P35005.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0088160 ; FBpp0087256 ; FBgn0010425 .
GeneIDi 49080.
KEGGi dme:Dmel_CG18681.
UCSCi CG18681-RA. d. melanogaster.

Organism-specific databases

CTDi 49080.
FlyBasei FBgn0010425. epsilonTry.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00620000088101.
InParanoidi P35005.
KOi K01312.
OMAi YSHDIVI.
OrthoDBi EOG7MKW6Q.
PhylomeDBi P35005.

Miscellaneous databases

GenomeRNAii 49080.
NextBioi 839635.

Gene expression databases

Bgeei P35005.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Concerted evolution within a trypsin gene cluster in Drosophila."
    Wang S., Magoulas C., Hickey D.A.
    Mol. Biol. Evol. 16:1117-1124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-R.
  2. Wang S., Magoulas C., Hickey D.A.
    Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-R.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiTRYE_DROME
AccessioniPrimary (citable) accession number: P35005
Secondary accession number(s): Q6IDF4, Q9V5Y1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3