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Protein

Trypsin epsilon

Gene

epsilonTry

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711Charge relay systemBy similarity
Active sitei116 – 1161Charge relay systemBy similarity
Sitei204 – 2041Required for specificityBy similarity
Active sitei210 – 2101Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.A82.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin epsilon (EC:3.4.21.4)
Gene namesi
Name:epsilonTry
Synonyms:TRY-EPSILON
ORF Names:CG18681
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0010425. epsilonTry.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222CuratedAdd
BLAST
Propeptidei23 – 308Activation peptidePRO_0000028273
Chaini31 – 256226Trypsin epsilonPRO_0000028274Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi56 ↔ 72PROSITE-ProRule annotation
Disulfide bondi180 ↔ 197PROSITE-ProRule annotation
Disulfide bondi206 ↔ 230PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP35005.
PRIDEiP35005.

Expressioni

Gene expression databases

BgeeiP35005.
GenevisibleiP35005. DM.

Interactioni

Protein-protein interaction databases

BioGridi71965. 2 interactions.
DIPiDIP-21329N.
IntActiP35005. 1 interaction.
MINTiMINT-907623.
STRINGi7227.FBpp0087256.

Structurei

3D structure databases

ProteinModelPortaliP35005.
SMRiP35005. Positions 31-253.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 254224Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00620000088101.
InParanoidiP35005.
KOiK01312.
OMAiYSHDIVI.
OrthoDBiEOG7MKW6Q.
PhylomeDBiP35005.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35005-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKFAVLLSV LACALAGTIP DGLLPQLDGR IVGGYETSID AHPYQVSLQR
60 70 80 90 100
YGSHFCGGSI YSHDIVITAA HCLQSIEAKD LKIRVGSTYW RSGGSVHSVR
110 120 130 140 150
SFRNHEGYNS RTMVNDIAII RIESDLSFRS SIREIRIADS NPREGATAVV
160 170 180 190 200
SGWGTTESGG STIPDHLLAV DLEIIDVSRC RSDEFGYGKK IKDTMLCAYA
210 220 230 240 250
PHKDACQGDS GGPLVSGDRL VGVVSWGYGC GDVRYPGVYA DVAHFHEWIE

RTAEEV
Length:256
Mass (Da):27,810
Last modified:February 1, 1994 - v1
Checksum:iCB8B276430F8616D
GO

Sequence cautioni

The sequence AAT27275.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96372 Genomic DNA. Translation: AAA28981.1.
U04853 Genomic DNA. Translation: AAA17452.1.
AE013599 Genomic DNA. Translation: AAF58660.1.
BT014651 mRNA. Translation: AAT27275.1. Different initiation.
RefSeqiNP_525112.1. NM_080373.3.
UniGeneiDm.1624.

Genome annotation databases

EnsemblMetazoaiFBtr0088160; FBpp0087256; FBgn0010425.
GeneIDi49080.
KEGGidme:Dmel_CG18681.
UCSCiCG18681-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96372 Genomic DNA. Translation: AAA28981.1.
U04853 Genomic DNA. Translation: AAA17452.1.
AE013599 Genomic DNA. Translation: AAF58660.1.
BT014651 mRNA. Translation: AAT27275.1. Different initiation.
RefSeqiNP_525112.1. NM_080373.3.
UniGeneiDm.1624.

3D structure databases

ProteinModelPortaliP35005.
SMRiP35005. Positions 31-253.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi71965. 2 interactions.
DIPiDIP-21329N.
IntActiP35005. 1 interaction.
MINTiMINT-907623.
STRINGi7227.FBpp0087256.

Protein family/group databases

MEROPSiS01.A82.

Proteomic databases

PaxDbiP35005.
PRIDEiP35005.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088160; FBpp0087256; FBgn0010425.
GeneIDi49080.
KEGGidme:Dmel_CG18681.
UCSCiCG18681-RA. d. melanogaster.

Organism-specific databases

CTDi49080.
FlyBaseiFBgn0010425. epsilonTry.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00620000088101.
InParanoidiP35005.
KOiK01312.
OMAiYSHDIVI.
OrthoDBiEOG7MKW6Q.
PhylomeDBiP35005.

Miscellaneous databases

GenomeRNAii49080.
NextBioi839635.
PROiP35005.

Gene expression databases

BgeeiP35005.
GenevisibleiP35005. DM.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Concerted evolution within a trypsin gene cluster in Drosophila."
    Wang S., Magoulas C., Hickey D.A.
    Mol. Biol. Evol. 16:1117-1124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-R.
  2. Wang S., Magoulas C., Hickey D.A.
    Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-R.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiTRYE_DROME
AccessioniPrimary (citable) accession number: P35005
Secondary accession number(s): Q6IDF4, Q9V5Y1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 22, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.