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Protein

Trypsin beta

Gene

betaTry

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711Charge relay systemBy similarity
Active sitei116 – 1161Charge relay systemBy similarity
Sitei204 – 2041Required for specificityBy similarity
Active sitei210 – 2101Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.A83.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin beta (EC:3.4.21.4)
Gene namesi
Name:betaTry
ORF Names:CG18211
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0010357. betaTry.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222CuratedAdd
BLAST
Propeptidei23 – 308Activation peptidePRO_0000028265
Chaini31 – 253223Trypsin betaPRO_0000028266Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi56 ↔ 72PROSITE-ProRule annotation
Disulfide bondi180 ↔ 197PROSITE-ProRule annotation
Disulfide bondi206 ↔ 230PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP35004.

Expressioni

Gene expression databases

BgeeiP35004.
GenevisibleiP35004. DM.

Interactioni

Protein-protein interaction databases

STRINGi7227.FBpp0087223.

Structurei

3D structure databases

ProteinModelPortaliP35004.
SMRiP35004. Positions 31-249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 253223Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG245986.
GeneTreeiENSGT00620000088101.
InParanoidiP35004.
KOiK01312.
OMAiIKSSMIC.
OrthoDBiEOG7MKW6Q.
PhylomeDBiP35004.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35004-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKFLILLSA VACALGGTIP EGLLPQLDGR IVGGTATTIS SFPWQISLQR
60 70 80 90 100
SGSHSCGGSI YSARVIVTAA HCLQSVSASS LQIRAGSSYW SSGGVVAKVS
110 120 130 140 150
SFKNHEGYNA NTMVNDIAVL HLSSSLSFSS TIKAIGLASS NPANGAAASV
160 170 180 190 200
SGWGTESSGS SSIPSQLRYV NVNIVSQSRC SSSSYGYGNQ IKSSMICAFA
210 220 230 240 250
SGKDSCQGDS GGPLVSGGVL VGVVSWGYGC AAANYPGVYA DVAALRSWVI

NNA
Length:253
Mass (Da):25,693
Last modified:August 16, 2005 - v2
Checksum:i3F5D35D235B8D8CD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531S → R in AAA28980 (PubMed:10486967).Curated
Sequence conflicti53 – 531S → R in AAA17451 (Ref. 2) Curated
Sequence conflicti85 – 851A → G in AAA28980 (PubMed:10486967).Curated
Sequence conflicti85 – 851A → G in AAA17451 (Ref. 2) Curated
Sequence conflicti114 – 1152VN → TS in AAA28980 (PubMed:10486967).Curated
Sequence conflicti114 – 1152VN → TS in AAA17451 (Ref. 2) Curated
Sequence conflicti121 – 1211H → N in AAA28980 (PubMed:10486967).Curated
Sequence conflicti121 – 1211H → N in AAA17451 (Ref. 2) Curated
Sequence conflicti142 – 1421P → T in AAA28980 (PubMed:10486967).Curated
Sequence conflicti142 – 1421P → T in AAA17451 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96372 Genomic DNA. Translation: AAA28980.1.
U04853 Genomic DNA. Translation: AAA17451.1.
AE013599 Genomic DNA. Translation: AAF58658.2.
BT016077 mRNA. Translation: AAV36962.1.
RefSeqiNP_001286314.1. NM_001299385.1.
NP_524904.1. NM_080165.3.
UniGeneiDm.11279.

Genome annotation databases

EnsemblMetazoaiFBtr0088122; FBpp0087223; FBgn0010357.
FBtr0345928; FBpp0311842; FBgn0010357.
GeneIDi47901.
KEGGidme:Dmel_CG18211.
UCSCiCG18211-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96372 Genomic DNA. Translation: AAA28980.1.
U04853 Genomic DNA. Translation: AAA17451.1.
AE013599 Genomic DNA. Translation: AAF58658.2.
BT016077 mRNA. Translation: AAV36962.1.
RefSeqiNP_001286314.1. NM_001299385.1.
NP_524904.1. NM_080165.3.
UniGeneiDm.11279.

3D structure databases

ProteinModelPortaliP35004.
SMRiP35004. Positions 31-249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7227.FBpp0087223.

Protein family/group databases

MEROPSiS01.A83.

Proteomic databases

PaxDbiP35004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088122; FBpp0087223; FBgn0010357.
FBtr0345928; FBpp0311842; FBgn0010357.
GeneIDi47901.
KEGGidme:Dmel_CG18211.
UCSCiCG18211-RA. d. melanogaster.

Organism-specific databases

CTDi47901.
FlyBaseiFBgn0010357. betaTry.

Phylogenomic databases

eggNOGiNOG245986.
GeneTreeiENSGT00620000088101.
InParanoidiP35004.
KOiK01312.
OMAiIKSSMIC.
OrthoDBiEOG7MKW6Q.
PhylomeDBiP35004.

Miscellaneous databases

GenomeRNAii47901.
NextBioi839220.
PROiP35004.

Gene expression databases

BgeeiP35004.
GenevisibleiP35004. DM.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Concerted evolution within a trypsin gene cluster in Drosophila."
    Wang S., Magoulas C., Hickey D.A.
    Mol. Biol. Evol. 16:1117-1124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-R.
  2. Wang S., Magoulas C., Hickey D.A.
    Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-R.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Larva and Pupae.

Entry informationi

Entry nameiTRYB_DROME
AccessioniPrimary (citable) accession number: P35004
Secondary accession number(s): Q9V5Y3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: August 16, 2005
Last modified: June 24, 2015
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.