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P35004

- TRYB_DROME

UniProt

P35004 - TRYB_DROME

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Protein

Trypsin beta

Gene
betaTry, CG18211
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711Charge relay system By similarity
Active sitei116 – 1161Charge relay system By similarity
Sitei204 – 2041Required for specificity By similarity
Active sitei210 – 2101Charge relay system By similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: FlyBase

GO - Biological processi

  1. proteolysis Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.A83.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin beta (EC:3.4.21.4)
Gene namesi
Name:betaTry
ORF Names:CG18211
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0010357. betaTry.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 InferredAdd
BLAST
Propeptidei23 – 308Activation peptidePRO_0000028265
Chaini31 – 253223Trypsin betaPRO_0000028266Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi56 ↔ 72 By similarity
Disulfide bondi180 ↔ 197 By similarity
Disulfide bondi206 ↔ 230 By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP35004.

Expressioni

Gene expression databases

BgeeiP35004.

Interactioni

Protein-protein interaction databases

STRINGi7227.FBpp0087223.

Structurei

3D structure databases

ProteinModelPortaliP35004.
SMRiP35004. Positions 31-249.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 253223Peptidase S1Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG245986.
GeneTreeiENSGT00620000088101.
InParanoidiP35004.
KOiK01312.
OMAiMICAFAS.
OrthoDBiEOG7MKW6Q.
PhylomeDBiP35004.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35004-1 [UniParc]FASTAAdd to Basket

« Hide

MLKFLILLSA VACALGGTIP EGLLPQLDGR IVGGTATTIS SFPWQISLQR    50
SGSHSCGGSI YSARVIVTAA HCLQSVSASS LQIRAGSSYW SSGGVVAKVS 100
SFKNHEGYNA NTMVNDIAVL HLSSSLSFSS TIKAIGLASS NPANGAAASV 150
SGWGTESSGS SSIPSQLRYV NVNIVSQSRC SSSSYGYGNQ IKSSMICAFA 200
SGKDSCQGDS GGPLVSGGVL VGVVSWGYGC AAANYPGVYA DVAALRSWVI 250
NNA 253
Length:253
Mass (Da):25,693
Last modified:August 16, 2005 - v2
Checksum:i3F5D35D235B8D8CD
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531S → R in AAA28980. 1 Publication
Sequence conflicti53 – 531S → R in AAA17451. 1 Publication
Sequence conflicti85 – 851A → G in AAA28980. 1 Publication
Sequence conflicti85 – 851A → G in AAA17451. 1 Publication
Sequence conflicti114 – 1152VN → TS in AAA28980. 1 Publication
Sequence conflicti114 – 1152VN → TS in AAA17451. 1 Publication
Sequence conflicti121 – 1211H → N in AAA28980. 1 Publication
Sequence conflicti121 – 1211H → N in AAA17451. 1 Publication
Sequence conflicti142 – 1421P → T in AAA28980. 1 Publication
Sequence conflicti142 – 1421P → T in AAA17451. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96372 Genomic DNA. Translation: AAA28980.1.
U04853 Genomic DNA. Translation: AAA17451.1.
AE013599 Genomic DNA. Translation: AAF58658.2.
BT016077 mRNA. Translation: AAV36962.1.
RefSeqiNP_524904.1. NM_080165.2.
UniGeneiDm.11279.

Genome annotation databases

EnsemblMetazoaiFBtr0088122; FBpp0087223; FBgn0010357.
GeneIDi47901.
KEGGidme:Dmel_CG18211.
UCSCiCG18211-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96372 Genomic DNA. Translation: AAA28980.1 .
U04853 Genomic DNA. Translation: AAA17451.1 .
AE013599 Genomic DNA. Translation: AAF58658.2 .
BT016077 mRNA. Translation: AAV36962.1 .
RefSeqi NP_524904.1. NM_080165.2.
UniGenei Dm.11279.

3D structure databases

ProteinModelPortali P35004.
SMRi P35004. Positions 31-249.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7227.FBpp0087223.

Protein family/group databases

MEROPSi S01.A83.

Proteomic databases

PaxDbi P35004.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0088122 ; FBpp0087223 ; FBgn0010357 .
GeneIDi 47901.
KEGGi dme:Dmel_CG18211.
UCSCi CG18211-RA. d. melanogaster.

Organism-specific databases

CTDi 47901.
FlyBasei FBgn0010357. betaTry.

Phylogenomic databases

eggNOGi NOG245986.
GeneTreei ENSGT00620000088101.
InParanoidi P35004.
KOi K01312.
OMAi MICAFAS.
OrthoDBi EOG7MKW6Q.
PhylomeDBi P35004.

Miscellaneous databases

GenomeRNAii 47901.
NextBioi 839220.

Gene expression databases

Bgeei P35004.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Concerted evolution within a trypsin gene cluster in Drosophila."
    Wang S., Magoulas C., Hickey D.A.
    Mol. Biol. Evol. 16:1117-1124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-R.
  2. Wang S., Magoulas C., Hickey D.A.
    Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-R.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Larva and Pupae.

Entry informationi

Entry nameiTRYB_DROME
AccessioniPrimary (citable) accession number: P35004
Secondary accession number(s): Q9V5Y3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: August 16, 2005
Last modified: July 9, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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