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P35004

- TRYB_DROME

UniProt

P35004 - TRYB_DROME

Protein

Trypsin beta

Gene

betaTry

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (16 Aug 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei71 – 711Charge relay systemBy similarity
    Active sitei116 – 1161Charge relay systemBy similarity
    Sitei204 – 2041Required for specificityBy similarity
    Active sitei210 – 2101Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: FlyBase

    GO - Biological processi

    1. proteolysis Source: FlyBase

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.A83.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin beta (EC:3.4.21.4)
    Gene namesi
    Name:betaTry
    ORF Names:CG18211
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0010357. betaTry.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222CuratedAdd
    BLAST
    Propeptidei23 – 308Activation peptidePRO_0000028265
    Chaini31 – 253223Trypsin betaPRO_0000028266Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi56 ↔ 72PROSITE-ProRule annotation
    Disulfide bondi180 ↔ 197PROSITE-ProRule annotation
    Disulfide bondi206 ↔ 230PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Proteomic databases

    PaxDbiP35004.

    Expressioni

    Gene expression databases

    BgeeiP35004.

    Interactioni

    Protein-protein interaction databases

    STRINGi7227.FBpp0087223.

    Structurei

    3D structure databases

    ProteinModelPortaliP35004.
    SMRiP35004. Positions 31-249.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 253223Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG245986.
    GeneTreeiENSGT00620000088101.
    InParanoidiP35004.
    KOiK01312.
    OMAiMICAFAS.
    OrthoDBiEOG7MKW6Q.
    PhylomeDBiP35004.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35004-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKFLILLSA VACALGGTIP EGLLPQLDGR IVGGTATTIS SFPWQISLQR    50
    SGSHSCGGSI YSARVIVTAA HCLQSVSASS LQIRAGSSYW SSGGVVAKVS 100
    SFKNHEGYNA NTMVNDIAVL HLSSSLSFSS TIKAIGLASS NPANGAAASV 150
    SGWGTESSGS SSIPSQLRYV NVNIVSQSRC SSSSYGYGNQ IKSSMICAFA 200
    SGKDSCQGDS GGPLVSGGVL VGVVSWGYGC AAANYPGVYA DVAALRSWVI 250
    NNA 253
    Length:253
    Mass (Da):25,693
    Last modified:August 16, 2005 - v2
    Checksum:i3F5D35D235B8D8CD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti53 – 531S → R in AAA28980. (PubMed:10486967)Curated
    Sequence conflicti53 – 531S → R in AAA17451. 1 PublicationCurated
    Sequence conflicti85 – 851A → G in AAA28980. (PubMed:10486967)Curated
    Sequence conflicti85 – 851A → G in AAA17451. 1 PublicationCurated
    Sequence conflicti114 – 1152VN → TS in AAA28980. (PubMed:10486967)Curated
    Sequence conflicti114 – 1152VN → TS in AAA17451. 1 PublicationCurated
    Sequence conflicti121 – 1211H → N in AAA28980. (PubMed:10486967)Curated
    Sequence conflicti121 – 1211H → N in AAA17451. 1 PublicationCurated
    Sequence conflicti142 – 1421P → T in AAA28980. (PubMed:10486967)Curated
    Sequence conflicti142 – 1421P → T in AAA17451. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96372 Genomic DNA. Translation: AAA28980.1.
    U04853 Genomic DNA. Translation: AAA17451.1.
    AE013599 Genomic DNA. Translation: AAF58658.2.
    BT016077 mRNA. Translation: AAV36962.1.
    RefSeqiNP_524904.1. NM_080165.2.
    UniGeneiDm.11279.

    Genome annotation databases

    EnsemblMetazoaiFBtr0088122; FBpp0087223; FBgn0010357.
    GeneIDi47901.
    KEGGidme:Dmel_CG18211.
    UCSCiCG18211-RA. d. melanogaster.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96372 Genomic DNA. Translation: AAA28980.1 .
    U04853 Genomic DNA. Translation: AAA17451.1 .
    AE013599 Genomic DNA. Translation: AAF58658.2 .
    BT016077 mRNA. Translation: AAV36962.1 .
    RefSeqi NP_524904.1. NM_080165.2.
    UniGenei Dm.11279.

    3D structure databases

    ProteinModelPortali P35004.
    SMRi P35004. Positions 31-249.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7227.FBpp0087223.

    Protein family/group databases

    MEROPSi S01.A83.

    Proteomic databases

    PaxDbi P35004.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0088122 ; FBpp0087223 ; FBgn0010357 .
    GeneIDi 47901.
    KEGGi dme:Dmel_CG18211.
    UCSCi CG18211-RA. d. melanogaster.

    Organism-specific databases

    CTDi 47901.
    FlyBasei FBgn0010357. betaTry.

    Phylogenomic databases

    eggNOGi NOG245986.
    GeneTreei ENSGT00620000088101.
    InParanoidi P35004.
    KOi K01312.
    OMAi MICAFAS.
    OrthoDBi EOG7MKW6Q.
    PhylomeDBi P35004.

    Miscellaneous databases

    GenomeRNAii 47901.
    NextBioi 839220.

    Gene expression databases

    Bgeei P35004.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Concerted evolution within a trypsin gene cluster in Drosophila."
      Wang S., Magoulas C., Hickey D.A.
      Mol. Biol. Evol. 16:1117-1124(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Oregon-R.
    2. Wang S., Magoulas C., Hickey D.A.
      Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Oregon-R.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    5. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Larva and Pupae.

    Entry informationi

    Entry nameiTRYB_DROME
    AccessioniPrimary (citable) accession number: P35004
    Secondary accession number(s): Q9V5Y3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: August 16, 2005
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3