ID   PE2R1_HUMAN             Reviewed;         402 AA.
AC   P34995; Q5U5U4; Q86UH3; Q86VB5; Q8NHB2;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 3.
DT   24-JAN-2024, entry version 181.
DE   RecName: Full=Prostaglandin E2 receptor EP1 subtype;
DE            Short=PGE receptor EP1 subtype;
DE            Short=PGE2 receptor EP1 subtype;
DE   AltName: Full=Prostanoid EP1 receptor;
GN   Name=PTGER1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT THR-71.
RX   PubMed=8253813; DOI=10.1016/s0021-9258(19)74379-8;
RA   Funk C.D., Furci L., Fitzgerald G.A., Grygorczyk R., Rochette C.,
RA   Bayne M.A., Abramovitz M., Adam M., Metters K.M.;
RT   "Cloning and expression of a cDNA for the human prostaglandin E receptor
RT   EP1 subtype.";
RL   J. Biol. Chem. 268:26767-26772(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S.,
RA   Tsutsumi S., Aburatani H., Asai K., Akiyama Y.;
RT   "Genome-wide discovery and analysis of human seven transmembrane helix
RT   receptor genes.";
RL   Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT THR-71.
RC   TISSUE=Kidney;
RA   Warren C.N., Aronstam R.S., Sharma S.V.;
RT   "cDNA clones of human proteins involved in signal transduction sequenced by
RT   the Guthrie cDNA resource center (www.cdna.org).";
RL   Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Ovary, and Pancreas;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Receptor for prostaglandin E2 (PGE2). The activity of this
CC       receptor is mediated by G(q) proteins which activate a
CC       phosphatidylinositol-calcium second messenger system. May play a role
CC       as an important modulator of renal function. Implicated the smooth
CC       muscle contractile response to PGE2 in various tissues.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Abundant in kidney. Lower level expression in lung,
CC       skeletal muscle and spleen, lowest expression in testis and not
CC       detected in liver brain and heart.
CC   -!- PTM: Phosphorylated. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAC05723.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; L22647; AAC37539.1; -; mRNA.
DR   EMBL; AB065464; BAC05723.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AY275470; AAP32302.1; -; mRNA.
DR   EMBL; BC039034; AAH39034.1; -; mRNA.
DR   EMBL; BC051286; AAH51286.1; -; mRNA.
DR   CCDS; CCDS12309.1; -.
DR   PIR; A49690; A49690.
DR   RefSeq; NP_000946.2; NM_000955.2.
DR   AlphaFoldDB; P34995; -.
DR   SMR; P34995; -.
DR   BioGRID; 111703; 4.
DR   STRING; 9606.ENSP00000292513; -.
DR   BindingDB; P34995; -.
DR   ChEMBL; CHEMBL1811; -.
DR   DrugBank; DB00770; Alprostadil.
DR   DrugBank; DB00905; Bimatoprost.
DR   DrugBank; DB00297; Bupivacaine.
DR   DrugBank; DB00429; Carboprost tromethamine.
DR   DrugBank; DB00917; Dinoprostone.
DR   DrugBank; DB01088; Iloprost.
DR   DrugBank; DB09211; Limaprost.
DR   DrugBank; DB00929; Misoprostol.
DR   DrugBank; DB15071; Omidenepag isopropyl.
DR   DrugBank; DB16315; Rivenprost.
DR   DrugBank; DB04297; Trichostatin A.
DR   DrugCentral; P34995; -.
DR   GuidetoPHARMACOLOGY; 340; -.
DR   TCDB; 9.A.14.9.1; the g-protein-coupled receptor (gpcr) family.
DR   GlyCosmos; P34995; 2 sites, No reported glycans.
DR   GlyGen; P34995; 2 sites.
DR   PhosphoSitePlus; P34995; -.
DR   BioMuta; PTGER1; -.
DR   DMDM; 83287886; -.
DR   PaxDb; 9606-ENSP00000292513; -.
DR   PeptideAtlas; P34995; -.
DR   Antibodypedia; 13701; 313 antibodies from 32 providers.
DR   DNASU; 5731; -.
DR   Ensembl; ENST00000292513.4; ENSP00000292513.3; ENSG00000160951.4.
DR   GeneID; 5731; -.
DR   KEGG; hsa:5731; -.
DR   MANE-Select; ENST00000292513.4; ENSP00000292513.3; NM_000955.3; NP_000946.2.
DR   UCSC; uc002mys.3; human.
DR   AGR; HGNC:9593; -.
DR   CTD; 5731; -.
DR   DisGeNET; 5731; -.
DR   GeneCards; PTGER1; -.
DR   HGNC; HGNC:9593; PTGER1.
DR   HPA; ENSG00000160951; Tissue enriched (kidney).
DR   MIM; 176802; gene.
DR   neXtProt; NX_P34995; -.
DR   OpenTargets; ENSG00000160951; -.
DR   PharmGKB; PA286; -.
DR   VEuPathDB; HostDB:ENSG00000160951; -.
DR   eggNOG; KOG3656; Eukaryota.
DR   GeneTree; ENSGT01030000234559; -.
DR   HOGENOM; CLU_045991_3_0_1; -.
DR   InParanoid; P34995; -.
DR   OMA; IYLIAKC; -.
DR   OrthoDB; 4642085at2759; -.
DR   PhylomeDB; P34995; -.
DR   TreeFam; TF324982; -.
DR   PathwayCommons; P34995; -.
DR   Reactome; R-HSA-391908; Prostanoid ligand receptors.
DR   Reactome; R-HSA-416476; G alpha (q) signalling events.
DR   SignaLink; P34995; -.
DR   SIGNOR; P34995; -.
DR   BioGRID-ORCS; 5731; 12 hits in 1156 CRISPR screens.
DR   GeneWiki; EP1_receptor; -.
DR   GenomeRNAi; 5731; -.
DR   Pharos; P34995; Tclin.
DR   PRO; PR:P34995; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   RNAct; P34995; Protein.
DR   Bgee; ENSG00000160951; Expressed in metanephros cortex and 96 other cell types or tissues.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0031748; F:D1 dopamine receptor binding; IEA:Ensembl.
DR   GO; GO:0004957; F:prostaglandin E receptor activity; IBA:GO_Central.
DR   GO; GO:0007191; P:adenylate cyclase-activating dopamine receptor signaling pathway; IEA:Ensembl.
DR   GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; TAS:ProtInc.
DR   GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR   GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR   GO; GO:0032496; P:response to lipopolysaccharide; IEA:Ensembl.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR000276; GPCR_Rhodpsn.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   InterPro; IPR008365; Prostanoid_rcpt.
DR   InterPro; IPR001244; Prostglndn_DP_rcpt.
DR   InterPro; IPR000708; Prostglndn_EP1_rcpt.
DR   PANTHER; PTHR11866; G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER; 1.
DR   PANTHER; PTHR11866:SF3; PROSTAGLANDIN E2 RECEPTOR EP1 SUBTYPE; 1.
DR   Pfam; PF00001; 7tm_1; 1.
DR   PRINTS; PR00428; PROSTAGLNDNR.
DR   PRINTS; PR01788; PROSTANOIDR.
DR   PRINTS; PR00580; PRSTNOIDEP1R.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR   Genevisible; P34995; HS.
PE   2: Evidence at transcript level;
KW   Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW   Membrane; Phosphoprotein; Receptor; Reference proteome; Transducer;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..402
FT                   /note="Prostaglandin E2 receptor EP1 subtype"
FT                   /id="PRO_0000070050"
FT   TOPO_DOM        1..35
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        36..62
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        63..72
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        73..96
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        97..111
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        112..133
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        134..155
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        156..177
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        178..201
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        202..227
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        228..294
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        295..321
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        322..332
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        333..354
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        355..402
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          238..266
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        8
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        25
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        110..188
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT   VARIANT         71
FT                   /note="A -> T (in dbSNP:rs1057362)"
FT                   /evidence="ECO:0000269|PubMed:8253813, ECO:0000269|Ref.3"
FT                   /id="VAR_017190"
FT   VARIANT         223
FT                   /note="T -> M (in dbSNP:rs28364042)"
FT                   /id="VAR_029216"
FT   VARIANT         256
FT                   /note="H -> R (in dbSNP:rs7249305)"
FT                   /id="VAR_029217"
FT   CONFLICT        230
FT                   /note="L -> H (in Ref. 1; AAC37539)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   402 AA;  41801 MW;  B925359ABD86880C CRC64;
     MSPCGPLNLS LAGEATTCAA PWVPNTSAVP PSGASPALPI FSMTLGAVSN LLALALLAQA
     AGRLRRRRSA ATFLLFVASL LATDLAGHVI PGALVLRLYT AGRAPAGGAC HFLGGCMVFF
     GLCPLLLGCG MAVERCVGVT RPLLHAARVS VARARLALAA VAAVALAVAL LPLARVGRYE
     LQYPGTWCFI GLGPPGGWRQ ALLAGLFASL GLVALLAALV CNTLSGLALL RARWRRRSRR
     PPPASGPDSR RRWGAHGPRS ASASSASSIA SASTFFGGSR SSGSARRARA HDVEMVGQLV
     GIMVVSCICW SPMLVLVALA VGGWSSTSLQ RPLFLAVRLA SWNQILDPWV YILLRQAVLR
     QLLRLLPPRA GAKGGPAGLG LTPSAWEASS LRSSRHSGLS HF
//