P34981 (TRFR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thyrotropin-releasing hormone receptor
Short name=TRH-R

Alternative name(s):
Thyroliberin receptor

Gene names

Name:

TRHR

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for thyrotropin-releasing hormone. This receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	thyrotropin-releasing hormone receptor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Thyrotropin-releasing hormone receptor		PRO_0000070187
Regions
	Topological domain	1 – 28	28	Extracellular Potential
	Transmembrane	29 – 51	23	Helical; Name=1; Potential
	Topological domain	52 – 61	10	Cytoplasmic Potential
	Transmembrane	62 – 83	22	Helical; Name=2; Potential
	Topological domain	84 – 99	16	Extracellular Potential
	Transmembrane	100 – 121	22	Helical; Name=3; Potential
	Topological domain	122 – 144	23	Cytoplasmic Potential
	Transmembrane	145 – 168	24	Helical; Name=4; Potential
	Topological domain	169 – 193	25	Extracellular Potential
	Transmembrane	194 – 215	22	Helical; Name=5; Potential
	Topological domain	216 – 266	51	Cytoplasmic Potential
	Transmembrane	267 – 288	22	Helical; Name=6; Potential
	Topological domain	289 – 296	8	Extracellular Potential
	Transmembrane	297 – 319	23	Helical; Name=7; Potential
	Topological domain	320 – 398	79	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	3	1	N-linked (GlcNAc...) Potential
	Glycosylation	10	1	N-linked (GlcNAc...) Potential
	Disulfide bond	98 ↔ 179		Ref.9
Natural variations
	Natural variant	10	1	N → K. Corresponds to variant rs5774 [ dbSNP | Ensembl ].		VAR_011857
	Natural variant	168	1	I → M. Corresponds to variant rs13306060 [ dbSNP | Ensembl ].		VAR_049450

Sequences

	Sequence		Length	Mass (Da)
	P34981 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: FE920B5F2E933D3E	FASTA	398	45,085
	10 20 30 40 50 60 MENETVSELN QTQLQPRAVV ALEYQVVTIL LVLIICGLGI VGNIMVVLVV MRTKHMRTPT 70 80 90 100 110 120 NCYLVSLAVA DLMVLVAAGL PNITDSIYGS WVYGYVGCLC ITYLQYLGIN ASSCSITAFT 130 140 150 160 170 180 IERYIAICHP IKAQFLCTFS RAKKIIIFVW AFTSLYCMLW FFLLDLNIST YKDAIVISCG 190 200 210 220 230 240 YKISRNYYSP IYLMDFGVFY VVPMILATVL YGFIARILFL NPIPSDPKEN SKTWKNDSTH 250 260 270 280 290 300 QNTNLNVNTS NRCFNSTVSS RKQVTKMLAV VVILFALLWM PYRTLVVVNS FLSSPFQENW 310 320 330 340 350 360 FLLFCRICIY LNSAINPVIY NLMSQKFRAA FRKLCNCKQK PTEKPANYSV ALNYSVIKES 370 380 390 DHFSTELDDI TVTDTYLSAT KVSFDDTCLA SEVSFSQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a functional human thyrotropin-releasing hormone receptor." Matre V., Karlsen H.E., Wright M.S., Lundell I., Fjeldheim K., Gabrielsen O.S., Larhammar D., Gautvik K.M. Biochem. Biophys. Res. Commun. 195:179-185(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Pituitary adenomas of patients with acromegaly express thyrotropin-releasing hormone receptor messenger RNA: cloning and functional expression of the human thyrotropin-releasing hormone receptor gene." Yamada M., Monden T., Satoh T., Satoh N., Murakami M., Iriuchijima T., Kakegawa T., Mori M. Biochem. Biophys. Res. Commun. 195:737-745(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary.
[3]	"Cloning and functional characterisation of the human TRH receptor." Duthie S.M., Taylor P.L., Anderson L., Cook J., Eidne K.A. Mol. Cell. Endocrinol. 95:R11-R15(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Molecular cloning and functional expression of a human thyrotropin-releasing hormone (TRH) receptor gene." Hinuma S., Hosoya M., Ogi K., Tanaka H., Nagai Y., Onda H. Biochim. Biophys. Acta 1219:251-259(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Genomic organization and promoter function of the human thyrotropin-releasing hormone receptor gene." Iwasaki T., Yamada M., Satoh T., Konaka S., Ren Y., Hashimoto K., Kohga H., Kato Y., Mori M. J. Biol. Chem. 271:22183-22188(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[8]	"Structural and functional organization of the gene encoding the human thyrotropin-releasing hormone receptor." Matre V., Hovring P.I., Orstavik S., Frengen E., Rian E., Velickovic Z., Murray-Mcintosh R.P., Gautvik K.M. J. Neurochem. 72:40-50(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-263. Tissue: Placenta.
[9]	"A disulfide bonding interaction role for cysteines in the extracellular domain of the thyrotropin-releasing hormone receptor." Cook J.V., McGregor A., Lee T., Milligan G., Eidne K.A. Endocrinology 137:2851-2858(1996) [PubMed] [Europe PMC] [Abstract] Cited for: DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D16845 mRNA. Translation: BAA04120.1. X75071 mRNA. Translation: CAA52965.1. X72089 mRNA. Translation: CAA50979.1. S75283, S75281 Genomic DNA. Translation: AAB32222.1. D85376 Genomic DNA. Translation: BAA12796.1. AY493373 mRNA. Translation: AAR84356.1. BC105045 mRNA. Translation: AAI05046.1. BC113360 mRNA. Translation: AAI13361.1. AJ011701 Genomic DNA. Translation: CAA09746.1.
IPI	IPI00003065.
PIR	JN0708. S40682.
RefSeq	NP_003292.1. NM_003301.5.
UniGene	Hs.3022.
3D structure databases
ProteinModelPortal	P34981.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000309818.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P34981.
Polymorphism databases
DMDM	464921.
Proteomic databases
PaxDb	P34981.
PRIDE	P34981.
Protocols and materials databases
DNASU	7201.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000311762; ENSP00000309818; ENSG00000174417. ENST00000518632; ENSP00000430711; ENSG00000174417.
GeneID	7201.
KEGG	hsa:7201.
UCSC	uc003ymz.4. human.
Organism-specific databases
CTD	7201.
GeneCards	GC08P110168.
HGNC	HGNC:12299. TRHR.
MIM	188545. gene+phenotype.
neXtProt	NX_P34981.
Orphanet	99832. Resistance to thyrotropin-releasing hormone syndrome.
PharmGKB	PA36979.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG303917.
HOGENOM	HOG000017249.
HOVERGEN	HBG007549.
InParanoid	P34981.
KO	K04282.
OMA	PTEKPAN.
OrthoDB	EOG4XKV75.
PhylomeDB	P34981.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	P34981.
CleanEx	HS_TRHR.
Genevestigator	P34981.
GermOnline	ENSG00000174417. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR009144. Thyrotropin_RHR. IPR002120. Trelhrm_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00751. THYROLIBRINR. PR01846. TRHRFAMILY.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P34981.
ChEMBL	CHEMBL1810.
GenomeRNAi	7201.
NextBio	28220.
SOURCE	Search...

Entry information

Entry name

TRFR_HUMAN

Accession

Primary (citable) accession number: P34981
Secondary accession number(s): Q2M339

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.