P34978 (TA2R_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thromboxane A2 receptor
Short name=TXA2-R

Alternative name(s):
Prostanoid TP receptor
TXR2

Gene names

Name:

Tbxa2r

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for thromboxane A2 (TXA2), a potent stimulator of platelet aggregation. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. In the kidney, the binding of TXA2 to glomerular TP receptors causes intense vasoconstriction. Activates phospholipase C and adenylyl cyclase.
Subunit structure	Interacts with RPGRIP1L. Interacts with GNB2L1/RACK1; the interaction regulates TBXA2R cell surface expression By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	In the brain, expressed in all types of glial cells. In the kidney, expressed in the mesangial cells of the glomerulus, smooth muscle cells of the renal arterioles, and in transitional cell epithelium of renal pelvis.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	elevation of cytosolic calcium ion concentration Inferred from direct assay Ref.1. Source: RGD inflammatory response Inferred from electronic annotation. Source: Compara positive regulation of angiogenesis Inferred from direct assay PubMed 16720756. Source: RGD positive regulation of blood pressure Inferred from mutant phenotype PubMed 16115588. Source: RGD positive regulation of smooth muscle contraction Inferred from electronic annotation. Source: Compara positive regulation of vasoconstriction Inferred from mutant phenotype PubMed 16905600. Source: RGD response to drug Inferred from expression pattern PubMed 11409645. Source: RGD response to lipopolysaccharide Inferred from electronic annotation. Source: Compara response to nutrient Inferred from expression pattern PubMed 14621185. Source: RGD second-messenger-mediated signaling Inferred from electronic annotation. Source: Compara water homeostasis Traceable author statement PubMed 11777901. Source: RGD
Cellular_component	acrosomal vesicle Inferred from direct assay PubMed 7649122. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thromboxane receptor activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Thromboxane A2 receptor		PRO_0000070140
Regions
	Topological domain	1 – 29	29	Extracellular Potential
	Transmembrane	30 – 52	23	Helical; Name=1; Potential
	Topological domain	53 – 65	13	Cytoplasmic Potential
	Transmembrane	66 – 86	21	Helical; Name=2; Potential
	Topological domain	87 – 105	19	Extracellular Potential
	Transmembrane	106 – 127	22	Helical; Name=3; Potential
	Topological domain	128 – 147	20	Cytoplasmic Potential
	Transmembrane	148 – 170	23	Helical; Name=4; Potential
	Topological domain	171 – 191	21	Extracellular Potential
	Transmembrane	192 – 217	26	Helical; Name=5; Potential
	Topological domain	218 – 244	27	Cytoplasmic Potential
	Transmembrane	245 – 268	24	Helical; Name=6; Potential
	Topological domain	269 – 287	19	Extracellular Potential
	Transmembrane	288 – 309	22	Helical; Name=7; Potential
	Topological domain	310 – 341	32	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	4	1	N-linked (GlcNAc...) Potential
	Glycosylation	16	1	N-linked (GlcNAc...) Potential
	Disulfide bond	104 ↔ 181		By similarity
Experimental info
	Sequence conflict	188	1	E → G in BAA06844. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P34978 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: E85843FE54C1CD94	FASTA	341	36,928
	10 20 30 40 50 60 MWLNSTSLGA CFRPVNITLQ ERRAIASPWF AASFCALGLG SNLLALSVLA GARPGAGPRS 70 80 90 100 110 120 SFLALLCGLV LTDFLGLLVT GAVVASQHAA LLDWRATDPG CRLCHFMGAA MVFFGLCPLL 130 140 150 160 170 180 LGAAMAAERF VGITRPFSRP AATSRRAWAT VGLVWVGAGT LGLLPLLGLG RYSVQYPGSW 190 200 210 220 230 240 CFLTLGAERG DVAFGLMFAL LGSVSVGLSL LLNTVSVATL CRVYHAREAT QRPRDCEVEM 250 260 270 280 290 300 MVQLVGIMVV ATVCWMPLLV FILQTLLQTL PVMSPSGQLL RTTERQLLIY LRVATWNQIL 310 320 330 340 DPWVYILFRR SVLRRLHPRF TSQLQAVSLH SPPTQAMLSG P

References

[1]	"Rat kidney thromboxane receptor: molecular cloning, signal transduction, and intrarenal expression localization." Abe T., Takeuchi K., Takahashi N., Tsutsumi E., Taniyama Y., Abe K. J. Clin. Invest. 96:657-664(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Kidney.
[2]	"cDNA cloning of a thromboxane A2 receptor from rat astrocytes." Kitanaka J., Hashimoto H., Sugimoto Y., Sawada M., Negishi M., Suzumura A., Marunouchi T., Ichikawa A., Baba A. Biochim. Biophys. Acta 1265:220-223(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[3]	"Characterization of a rat kidney thromboxane A2 receptor: high affinity for the agonist ligand I-BOP." D'Angelo D.D., Terasawa T., Carlisle S.J., Dorn G.W. II, Lynch K.R. Prostaglandins 52:303-316(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D21158 mRNA. Translation: BAA04694.1. D32080 mRNA. Translation: BAA06844.1.
IPI	IPI00325303.
PIR	I55623.
RefSeq	NP_058750.1. NM_017054.1.
UniGene	Rn.44614.
3D structure databases
ProteinModelPortal	P34978.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000027932.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P34978.
Proteomic databases
PaxDb	P34978.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000027932; ENSRNOP00000027932; ENSRNOG00000020585.
GeneID	24816.
KEGG	rno:24816.
UCSC	RGD:3825. rat.
Organism-specific databases
CTD	6915.
RGD	3825. Tbxa2r.
Phylogenomic databases
eggNOG	NOG263172.
GeneTree	ENSGT00690000102015.
HOGENOM	HOG000015238.
HOVERGEN	HBG108543.
InParanoid	P34978.
KO	K04264.
OMA	EMMVQLL.
OrthoDB	EOG49S674.
Gene expression databases
Genevestigator	P34978.
GermOnline	ENSRNOG00000020585. Rattus norvegicus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR008365. Prostanoid_rcpt. IPR001105. Thbox_rcpt. [Graphical view]
PANTHER	PTHR11866. PTHR11866. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01788. PROSTANOIDR. PR00429. THROMBOXANER.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P34978.
ChEMBL	CHEMBL3156.
NextBio	604510.

Entry information

Entry name

TA2R_RAT

Accession

Primary (citable) accession number: P34978

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program