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Protein

Kappa-type opioid receptor

Gene

Oprk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled opioid receptor that functions as receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions.2 Publications

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-inhibiting opioid receptor signaling pathway Source: UniProtKB
  • behavioral response to cocaine Source: RGD
  • cellular response to lipopolysaccharide Source: RGD
  • defense response to virus Source: Ensembl
  • eating behavior Source: RGD
  • estrous cycle Source: RGD
  • G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
  • immune response Source: Ensembl
  • locomotory behavior Source: UniProtKB
  • maternal behavior Source: RGD
  • negative regulation of luteinizing hormone secretion Source: RGD
  • neuropeptide signaling pathway Source: GO_Central
  • opioid receptor signaling pathway Source: GO_Central
  • phospholipase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
  • positive regulation of dopamine secretion Source: RGD
  • positive regulation of locomotion Source: RGD
  • positive regulation of p38MAPK cascade Source: RGD
  • positive regulation of potassium ion transmembrane transport Source: RGD
  • regulation of aerobic respiration Source: RGD
  • regulation of energy homeostasis Source: RGD
  • regulation of saliva secretion Source: UniProtKB
  • regulation of sensory perception of pain Source: RGD
  • response to acrylamide Source: RGD
  • response to cocaine Source: RGD
  • response to estrogen Source: RGD
  • response to ethanol Source: RGD
  • response to insulin Source: RGD
  • response to morphine Source: RGD
  • response to radiation Source: RGD
  • sensory perception of pain Source: UniProtKB
  • sensory perception of temperature stimulus Source: RGD
  • synaptic transmission Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Enzyme and pathway databases

ReactomeiREACT_306851. Peptide ligand-binding receptors.
REACT_339234. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-type opioid receptor
Short name:
K-OR-1
Short name:
KOR-1
Gene namesi
Name:Oprk1
Synonyms:Ror-d
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi69426. Oprk1.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5757ExtracellularBy similarityAdd
BLAST
Transmembranei58 – 8528Helical; Name=1By similarityAdd
BLAST
Topological domaini86 – 9510CytoplasmicBy similarity
Transmembranei96 – 11924Helical; Name=2By similarityAdd
BLAST
Topological domaini120 – 13213ExtracellularBy similarityAdd
BLAST
Transmembranei133 – 15422Helical; Name=3By similarityAdd
BLAST
Topological domaini155 – 17319CytoplasmicBy similarityAdd
BLAST
Transmembranei174 – 19623Helical; Name=4By similarityAdd
BLAST
Topological domaini197 – 22226ExtracellularBy similarityAdd
BLAST
Transmembranei223 – 24725Helical; Name=5By similarityAdd
BLAST
Topological domaini248 – 27427CytoplasmicBy similarityAdd
BLAST
Transmembranei275 – 29622Helical; Name=6By similarityAdd
BLAST
Topological domaini297 – 31115ExtracellularBy similarityAdd
BLAST
Transmembranei312 – 33322Helical; Name=7By similarityAdd
BLAST
Topological domaini334 – 38047CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • axon terminus Source: RGD
  • dendrite Source: RGD
  • integral component of membrane Source: UniProtKB
  • integral component of plasma membrane Source: UniProtKB
  • neuronal cell body Source: RGD
  • neuron projection Source: GO_Central
  • perikaryon Source: RGD
  • plasma membrane Source: RGD
  • synapse Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Kappa-type opioid receptorPRO_0000069969Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi25 – 251N-linked (GlcNAc...)By similarity
Glycosylationi39 – 391N-linked (GlcNAc...)By similarity
Disulfide bondi131 ↔ 210PROSITE-ProRule annotation
Lipidationi345 – 3451S-palmitoyl cysteineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

PTM databases

PhosphoSiteiP34975.

Expressioni

Gene expression databases

GenevestigatoriP34975.

Interactioni

Subunit structurei

Interacts with SLC9A3R1. Interacts with GABARAPL1 (By similarity).By similarity

Protein-protein interaction databases

IntActiP34975. 1 interaction.
STRINGi10116.ENSRNOP00000010255.

Structurei

3D structure databases

ProteinModelPortaliP34975.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG317066.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230486.
HOVERGENiHBG106919.
InParanoidiP34975.
KOiK04214.
OMAiPFQSTEY.
OrthoDBiEOG7BKCVQ.
PhylomeDBiP34975.
TreeFamiTF315737.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000452. Kappa_opi_rcpt.
IPR001418. Opioid_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00532. KAPPAOPIOIDR.
PR00384. OPIOIDR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34975-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESPIQIFRG EPGPTCAPSA CLLPNSSSWF PNWAESDSNG SVGSEDQQLE
60 70 80 90 100
PAHISPAIPV IITAVYSVVF VVGLVGNSLV MFVIIRYTKM KTATNIYIFN
110 120 130 140 150
LALADALVTT TMPFQSAVYL MNSWPFGDVL CKIVISIDYY NMFTSIFTLT
160 170 180 190 200
MMSVDRYIAV CHPVKALDFR TPLKAKIINI CIWLLASSVG ISAIVLGGTK
210 220 230 240 250
VREDVDVIEC SLQFPDDEYS WWDLFMKICV FVFAFVIPVL IIIVCYTLMI
260 270 280 290 300
LRLKSVRLLS GSREKDRNLR RITKLVLVVV AVFIICWTPI HIFILVEALG
310 320 330 340 350
STSHSTAVLS SYYFCIALGY TNSSLNPVLY AFLDENFKRC FRDFCFPIKM
360 370 380
RMERQSTNRV RNTVQDPASM RDVGGMNKPV
Length:380
Mass (Da):42,688
Last modified:February 1, 1994 - v1
Checksum:i107FEEDB09886F92
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti42 – 421V → L in BAA04109 (PubMed:8103466).Curated
Sequence conflicti345 – 3451C → Y in AAA41496 (PubMed:8240268).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22001 mRNA. Translation: AAA41495.1.
D16829 mRNA. Translation: BAA04109.1.
L22536 mRNA. Translation: AAA41496.1.
U00442 mRNA. Translation: AAA18261.1.
D16534 mRNA. Translation: BAA03971.1.
U17995, U17993, U17994 Genomic DNA. Translation: AAC53249.1.
PIRiS36143.
RefSeqiNP_058863.1. NM_017167.2.
UniGeneiRn.89571.

Genome annotation databases

EnsembliENSRNOT00000010254; ENSRNOP00000010255; ENSRNOG00000007647.
GeneIDi29335.
KEGGirno:29335.
UCSCiRGD:69426. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22001 mRNA. Translation: AAA41495.1.
D16829 mRNA. Translation: BAA04109.1.
L22536 mRNA. Translation: AAA41496.1.
U00442 mRNA. Translation: AAA18261.1.
D16534 mRNA. Translation: BAA03971.1.
U17995, U17993, U17994 Genomic DNA. Translation: AAC53249.1.
PIRiS36143.
RefSeqiNP_058863.1. NM_017167.2.
UniGeneiRn.89571.

3D structure databases

ProteinModelPortaliP34975.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP34975. 1 interaction.
STRINGi10116.ENSRNOP00000010255.

Chemistry

BindingDBiP34975.
ChEMBLiCHEMBL2094129.
GuidetoPHARMACOLOGYi318.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP34975.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010254; ENSRNOP00000010255; ENSRNOG00000007647.
GeneIDi29335.
KEGGirno:29335.
UCSCiRGD:69426. rat.

Organism-specific databases

CTDi4986.
RGDi69426. Oprk1.

Phylogenomic databases

eggNOGiNOG317066.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230486.
HOVERGENiHBG106919.
InParanoidiP34975.
KOiK04214.
OMAiPFQSTEY.
OrthoDBiEOG7BKCVQ.
PhylomeDBiP34975.
TreeFamiTF315737.

Enzyme and pathway databases

ReactomeiREACT_306851. Peptide ligand-binding receptors.
REACT_339234. G alpha (i) signalling events.

Miscellaneous databases

NextBioi608812.
PROiP34975.

Gene expression databases

GenevestigatoriP34975.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000452. Kappa_opi_rcpt.
IPR001418. Opioid_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00532. KAPPAOPIOIDR.
PR00384. OPIOIDR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of a rat kappa opioid receptor reveals sequence similarities to the mu and delta opioid receptors."
    Chen Y., Mestek A., Liu J., Yu L.
    Biochem. J. 295:625-628(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Tissue: Brain.
  2. "Cloning and expression of a cDNA for the rat kappa-opioid receptor."
    Minami M., Toya T., Katao Y., Maekawa K., Nakamura S., Onogi T., Kaneko S., Satoh M.
    FEBS Lett. 329:291-295(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Molecular cloning and expression of a rat kappa opioid receptor."
    Li S., Zhu J., Chen C., Chen Y.-W., Deriel J.K., Ashby B., Liu-Chen L.-Y.
    Biochem. J. 295:629-633(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  4. "Cloning and pharmacological characterization of a rat kappa opioid receptor."
    Meng F., Xie G.-X., Thompson R.C., Mansour A., Goldstein A., Watson S.J., Akil H.
    Proc. Natl. Acad. Sci. U.S.A. 90:9954-9958(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  5. "cDNA cloning and pharmacological characterization of an opioid receptor with high affinities for kappa-subtype-selective ligands."
    Nishi M., Takeshima H., Fukuda K., Kato S., Mori K.
    FEBS Lett. 330:77-80(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  6. "Structure and expression of a rat kappa opioid receptor gene."
    Yakovlev A.G., Krueger K.E., Faden A.I.
    J. Biol. Chem. 270:6421-6424(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
  7. "Role of kappa-opioid receptors in the effects of salvinorin A and ketamine on attention in rats."
    Nemeth C.L., Paine T.A., Rittiner J.E., Beguin C., Carroll F.I., Roth B.L., Cohen B.M., Carlezon W.A. Jr.
    Psychopharmacology 210:263-274(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiOPRK_RAT
AccessioniPrimary (citable) accession number: P34975
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 27, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.