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P34975 (OPRK_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kappa-type opioid receptor

Short name=K-OR-1
Short name=KOR-1
Gene names
Name:Oprk1
Synonyms:Ror-d
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length380 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

G-protein coupled opioid receptor that functions as receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions. Ref.1 Ref.7

Subunit structure

Interacts with SLC9A3R1. Interacts with GABARAPL1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.1.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 380380Kappa-type opioid receptor
PRO_0000069969

Regions

Topological domain1 – 5757Extracellular By similarity
Transmembrane58 – 8528Helical; Name=1; By similarity
Topological domain86 – 9510Cytoplasmic By similarity
Transmembrane96 – 11924Helical; Name=2; By similarity
Topological domain120 – 13213Extracellular By similarity
Transmembrane133 – 15422Helical; Name=3; By similarity
Topological domain155 – 17319Cytoplasmic By similarity
Transmembrane174 – 19623Helical; Name=4; By similarity
Topological domain197 – 22226Extracellular By similarity
Transmembrane223 – 24725Helical; Name=5; By similarity
Topological domain248 – 27427Cytoplasmic By similarity
Transmembrane275 – 29622Helical; Name=6; By similarity
Topological domain297 – 31115Extracellular By similarity
Transmembrane312 – 33322Helical; Name=7; By similarity
Topological domain334 – 38047Cytoplasmic By similarity

Amino acid modifications

Lipidation3451S-palmitoyl cysteine Potential
Glycosylation251N-linked (GlcNAc...) By similarity
Glycosylation391N-linked (GlcNAc...) By similarity
Disulfide bond131 ↔ 210 By similarity

Experimental info

Sequence conflict421V → L in BAA04109. Ref.2
Sequence conflict3451C → Y in AAA41496. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P34975 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 107FEEDB09886F92

FASTA38042,688
        10         20         30         40         50         60 
MESPIQIFRG EPGPTCAPSA CLLPNSSSWF PNWAESDSNG SVGSEDQQLE PAHISPAIPV 

        70         80         90        100        110        120 
IITAVYSVVF VVGLVGNSLV MFVIIRYTKM KTATNIYIFN LALADALVTT TMPFQSAVYL 

       130        140        150        160        170        180 
MNSWPFGDVL CKIVISIDYY NMFTSIFTLT MMSVDRYIAV CHPVKALDFR TPLKAKIINI 

       190        200        210        220        230        240 
CIWLLASSVG ISAIVLGGTK VREDVDVIEC SLQFPDDEYS WWDLFMKICV FVFAFVIPVL 

       250        260        270        280        290        300 
IIIVCYTLMI LRLKSVRLLS GSREKDRNLR RITKLVLVVV AVFIICWTPI HIFILVEALG 

       310        320        330        340        350        360 
STSHSTAVLS SYYFCIALGY TNSSLNPVLY AFLDENFKRC FRDFCFPIKM RMERQSTNRV 

       370        380 
RNTVQDPASM RDVGGMNKPV 

« Hide

References

[1]"Molecular cloning of a rat kappa opioid receptor reveals sequence similarities to the mu and delta opioid receptors."
Chen Y., Mestek A., Liu J., Yu L.
Biochem. J. 295:625-628(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
Tissue: Brain.
[2]"Cloning and expression of a cDNA for the rat kappa-opioid receptor."
Minami M., Toya T., Katao Y., Maekawa K., Nakamura S., Onogi T., Kaneko S., Satoh M.
FEBS Lett. 329:291-295(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Molecular cloning and expression of a rat kappa opioid receptor."
Li S., Zhu J., Chen C., Chen Y.-W., Deriel J.K., Ashby B., Liu-Chen L.-Y.
Biochem. J. 295:629-633(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[4]"Cloning and pharmacological characterization of a rat kappa opioid receptor."
Meng F., Xie G.-X., Thompson R.C., Mansour A., Goldstein A., Watson S.J., Akil H.
Proc. Natl. Acad. Sci. U.S.A. 90:9954-9958(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[5]"cDNA cloning and pharmacological characterization of an opioid receptor with high affinities for kappa-subtype-selective ligands."
Nishi M., Takeshima H., Fukuda K., Kato S., Mori K.
FEBS Lett. 330:77-80(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Brain.
[6]"Structure and expression of a rat kappa opioid receptor gene."
Yakovlev A.G., Krueger K.E., Faden A.I.
J. Biol. Chem. 270:6421-6424(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
[7]"Role of kappa-opioid receptors in the effects of salvinorin A and ketamine on attention in rats."
Nemeth C.L., Paine T.A., Rittiner J.E., Beguin C., Carroll F.I., Roth B.L., Cohen B.M., Carlezon W.A. Jr.
Psychopharmacology 210:263-274(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L22001 mRNA. Translation: AAA41495.1.
D16829 mRNA. Translation: BAA04109.1.
L22536 mRNA. Translation: AAA41496.1.
U00442 mRNA. Translation: AAA18261.1.
D16534 mRNA. Translation: BAA03971.1.
U17995, U17993, U17994 Genomic DNA. Translation: AAC53249.1.
PIRS36143.
RefSeqNP_058863.1. NM_017167.2.
UniGeneRn.89571.

3D structure databases

ProteinModelPortalP34975.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP34975. 1 interaction.
STRING10116.ENSRNOP00000010255.

Chemistry

BindingDBP34975.
ChEMBLCHEMBL2094129.
GuidetoPHARMACOLOGY318.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP34975.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000010254; ENSRNOP00000010255; ENSRNOG00000007647.
GeneID29335.
KEGGrno:29335.
UCSCRGD:69426. rat.

Organism-specific databases

CTD4986.
RGD69426. Oprk1.

Phylogenomic databases

eggNOGNOG317066.
GeneTreeENSGT00630000089574.
HOGENOMHOG000230486.
HOVERGENHBG106919.
InParanoidP34975.
KOK04214.
OMADVDGMNK.
OrthoDBEOG7BKCVQ.
PhylomeDBP34975.
TreeFamTF315737.

Gene expression databases

GenevestigatorP34975.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000452. Kappa_opi_rcpt.
IPR001418. Opioid_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00532. KAPPAOPIOIDR.
PR00384. OPIOIDR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio608812.
PROP34975.

Entry information

Entry nameOPRK_RAT
AccessionPrimary (citable) accession number: P34975
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries