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P34975

- OPRK_RAT

UniProt

P34975 - OPRK_RAT

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Protein

Kappa-type opioid receptor

Gene

Oprk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

G-protein coupled opioid receptor that functions as receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions.2 Publications

GO - Molecular functioni

  1. dynorphin receptor activity Source: UniProtKB
  2. opioid receptor activity Source: UniProtKB

GO - Biological processi

  1. adenylate cyclase-inhibiting opioid receptor signaling pathway Source: UniProtKB
  2. defense response to virus Source: Ensembl
  3. immune response Source: Ensembl
  4. locomotory behavior Source: UniProtKB
  5. phospholipase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
  6. regulation of saliva secretion Source: UniProtKB
  7. sensory perception of pain Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Enzyme and pathway databases

ReactomeiREACT_233455. G alpha (i) signalling events.
REACT_250028. Peptide ligand-binding receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-type opioid receptor
Short name:
K-OR-1
Short name:
KOR-1
Gene namesi
Name:Oprk1
Synonyms:Ror-d
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 5

Organism-specific databases

RGDi69426. Oprk1.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5757ExtracellularBy similarityAdd
BLAST
Transmembranei58 – 8528Helical; Name=1By similarityAdd
BLAST
Topological domaini86 – 9510CytoplasmicBy similarity
Transmembranei96 – 11924Helical; Name=2By similarityAdd
BLAST
Topological domaini120 – 13213ExtracellularBy similarityAdd
BLAST
Transmembranei133 – 15422Helical; Name=3By similarityAdd
BLAST
Topological domaini155 – 17319CytoplasmicBy similarityAdd
BLAST
Transmembranei174 – 19623Helical; Name=4By similarityAdd
BLAST
Topological domaini197 – 22226ExtracellularBy similarityAdd
BLAST
Transmembranei223 – 24725Helical; Name=5By similarityAdd
BLAST
Topological domaini248 – 27427CytoplasmicBy similarityAdd
BLAST
Transmembranei275 – 29622Helical; Name=6By similarityAdd
BLAST
Topological domaini297 – 31115ExtracellularBy similarityAdd
BLAST
Transmembranei312 – 33322Helical; Name=7By similarityAdd
BLAST
Topological domaini334 – 38047CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB
  2. integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Kappa-type opioid receptorPRO_0000069969Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi25 – 251N-linked (GlcNAc...)By similarity
Glycosylationi39 – 391N-linked (GlcNAc...)By similarity
Disulfide bondi131 ↔ 210PROSITE-ProRule annotation
Lipidationi345 – 3451S-palmitoyl cysteineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

PTM databases

PhosphoSiteiP34975.

Expressioni

Gene expression databases

GenevestigatoriP34975.

Interactioni

Subunit structurei

Interacts with SLC9A3R1. Interacts with GABARAPL1 (By similarity).By similarity

Protein-protein interaction databases

IntActiP34975. 1 interaction.
STRINGi10116.ENSRNOP00000010255.

Structurei

3D structure databases

ProteinModelPortaliP34975.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG317066.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230486.
HOVERGENiHBG106919.
InParanoidiP34975.
KOiK04214.
OMAiPFQSTEY.
OrthoDBiEOG7BKCVQ.
PhylomeDBiP34975.
TreeFamiTF315737.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000452. Kappa_opi_rcpt.
IPR001418. Opioid_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00532. KAPPAOPIOIDR.
PR00384. OPIOIDR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34975-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESPIQIFRG EPGPTCAPSA CLLPNSSSWF PNWAESDSNG SVGSEDQQLE
60 70 80 90 100
PAHISPAIPV IITAVYSVVF VVGLVGNSLV MFVIIRYTKM KTATNIYIFN
110 120 130 140 150
LALADALVTT TMPFQSAVYL MNSWPFGDVL CKIVISIDYY NMFTSIFTLT
160 170 180 190 200
MMSVDRYIAV CHPVKALDFR TPLKAKIINI CIWLLASSVG ISAIVLGGTK
210 220 230 240 250
VREDVDVIEC SLQFPDDEYS WWDLFMKICV FVFAFVIPVL IIIVCYTLMI
260 270 280 290 300
LRLKSVRLLS GSREKDRNLR RITKLVLVVV AVFIICWTPI HIFILVEALG
310 320 330 340 350
STSHSTAVLS SYYFCIALGY TNSSLNPVLY AFLDENFKRC FRDFCFPIKM
360 370 380
RMERQSTNRV RNTVQDPASM RDVGGMNKPV
Length:380
Mass (Da):42,688
Last modified:February 1, 1994 - v1
Checksum:i107FEEDB09886F92
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti42 – 421V → L in BAA04109. (PubMed:8103466)Curated
Sequence conflicti345 – 3451C → Y in AAA41496. (PubMed:8240268)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22001 mRNA. Translation: AAA41495.1.
D16829 mRNA. Translation: BAA04109.1.
L22536 mRNA. Translation: AAA41496.1.
U00442 mRNA. Translation: AAA18261.1.
D16534 mRNA. Translation: BAA03971.1.
U17995, U17993, U17994 Genomic DNA. Translation: AAC53249.1.
PIRiS36143.
RefSeqiNP_058863.1. NM_017167.2.
UniGeneiRn.89571.

Genome annotation databases

EnsembliENSRNOT00000010254; ENSRNOP00000010255; ENSRNOG00000007647.
GeneIDi29335.
KEGGirno:29335.
UCSCiRGD:69426. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22001 mRNA. Translation: AAA41495.1 .
D16829 mRNA. Translation: BAA04109.1 .
L22536 mRNA. Translation: AAA41496.1 .
U00442 mRNA. Translation: AAA18261.1 .
D16534 mRNA. Translation: BAA03971.1 .
U17995 , U17993 , U17994 Genomic DNA. Translation: AAC53249.1 .
PIRi S36143.
RefSeqi NP_058863.1. NM_017167.2.
UniGenei Rn.89571.

3D structure databases

ProteinModelPortali P34975.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P34975. 1 interaction.
STRINGi 10116.ENSRNOP00000010255.

Chemistry

BindingDBi P34975.
ChEMBLi CHEMBL2094129.
GuidetoPHARMACOLOGYi 318.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P34975.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000010254 ; ENSRNOP00000010255 ; ENSRNOG00000007647 .
GeneIDi 29335.
KEGGi rno:29335.
UCSCi RGD:69426. rat.

Organism-specific databases

CTDi 4986.
RGDi 69426. Oprk1.

Phylogenomic databases

eggNOGi NOG317066.
GeneTreei ENSGT00760000118797.
HOGENOMi HOG000230486.
HOVERGENi HBG106919.
InParanoidi P34975.
KOi K04214.
OMAi PFQSTEY.
OrthoDBi EOG7BKCVQ.
PhylomeDBi P34975.
TreeFami TF315737.

Enzyme and pathway databases

Reactomei REACT_233455. G alpha (i) signalling events.
REACT_250028. Peptide ligand-binding receptors.

Miscellaneous databases

NextBioi 608812.
PROi P34975.

Gene expression databases

Genevestigatori P34975.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000452. Kappa_opi_rcpt.
IPR001418. Opioid_rcpt.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PR00532. KAPPAOPIOIDR.
PR00384. OPIOIDR.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of a rat kappa opioid receptor reveals sequence similarities to the mu and delta opioid receptors."
    Chen Y., Mestek A., Liu J., Yu L.
    Biochem. J. 295:625-628(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Tissue: Brain.
  2. "Cloning and expression of a cDNA for the rat kappa-opioid receptor."
    Minami M., Toya T., Katao Y., Maekawa K., Nakamura S., Onogi T., Kaneko S., Satoh M.
    FEBS Lett. 329:291-295(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Molecular cloning and expression of a rat kappa opioid receptor."
    Li S., Zhu J., Chen C., Chen Y.-W., Deriel J.K., Ashby B., Liu-Chen L.-Y.
    Biochem. J. 295:629-633(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  4. "Cloning and pharmacological characterization of a rat kappa opioid receptor."
    Meng F., Xie G.-X., Thompson R.C., Mansour A., Goldstein A., Watson S.J., Akil H.
    Proc. Natl. Acad. Sci. U.S.A. 90:9954-9958(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  5. "cDNA cloning and pharmacological characterization of an opioid receptor with high affinities for kappa-subtype-selective ligands."
    Nishi M., Takeshima H., Fukuda K., Kato S., Mori K.
    FEBS Lett. 330:77-80(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  6. "Structure and expression of a rat kappa opioid receptor gene."
    Yakovlev A.G., Krueger K.E., Faden A.I.
    J. Biol. Chem. 270:6421-6424(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
  7. "Role of kappa-opioid receptors in the effects of salvinorin A and ketamine on attention in rats."
    Nemeth C.L., Paine T.A., Rittiner J.E., Beguin C., Carroll F.I., Roth B.L., Cohen B.M., Carlezon W.A. Jr.
    Psychopharmacology 210:263-274(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiOPRK_RAT
AccessioniPrimary (citable) accession number: P34975
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 26, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3