Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P34975

- OPRK_RAT

UniProt

P34975 - OPRK_RAT

Protein

Kappa-type opioid receptor

Gene

Oprk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    G-protein coupled opioid receptor that functions as receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions.2 Publications

    GO - Molecular functioni

    1. dynorphin receptor activity Source: UniProtKB
    2. opioid receptor activity Source: UniProtKB

    GO - Biological processi

    1. adenylate cyclase-inhibiting opioid receptor signaling pathway Source: UniProtKB
    2. defense response to virus Source: Ensembl
    3. immune response Source: Ensembl
    4. locomotory behavior Source: UniProtKB
    5. phospholipase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
    6. regulation of saliva secretion Source: UniProtKB
    7. sensory perception of pain Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Behavior

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kappa-type opioid receptor
    Short name:
    K-OR-1
    Short name:
    KOR-1
    Gene namesi
    Name:Oprk1
    Synonyms:Ror-d
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 5

    Organism-specific databases

    RGDi69426. Oprk1.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB
    2. integral component of plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 380380Kappa-type opioid receptorPRO_0000069969Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi25 – 251N-linked (GlcNAc...)By similarity
    Glycosylationi39 – 391N-linked (GlcNAc...)By similarity
    Disulfide bondi131 ↔ 210PROSITE-ProRule annotation
    Lipidationi345 – 3451S-palmitoyl cysteineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    PTM databases

    PhosphoSiteiP34975.

    Expressioni

    Gene expression databases

    GenevestigatoriP34975.

    Interactioni

    Subunit structurei

    Interacts with SLC9A3R1. Interacts with GABARAPL1 By similarity.By similarity

    Protein-protein interaction databases

    IntActiP34975. 1 interaction.
    STRINGi10116.ENSRNOP00000010255.

    Structurei

    3D structure databases

    ProteinModelPortaliP34975.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5757ExtracellularBy similarityAdd
    BLAST
    Topological domaini86 – 9510CytoplasmicBy similarity
    Topological domaini120 – 13213ExtracellularBy similarityAdd
    BLAST
    Topological domaini155 – 17319CytoplasmicBy similarityAdd
    BLAST
    Topological domaini197 – 22226ExtracellularBy similarityAdd
    BLAST
    Topological domaini248 – 27427CytoplasmicBy similarityAdd
    BLAST
    Topological domaini297 – 31115ExtracellularBy similarityAdd
    BLAST
    Topological domaini334 – 38047CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei58 – 8528Helical; Name=1By similarityAdd
    BLAST
    Transmembranei96 – 11924Helical; Name=2By similarityAdd
    BLAST
    Transmembranei133 – 15422Helical; Name=3By similarityAdd
    BLAST
    Transmembranei174 – 19623Helical; Name=4By similarityAdd
    BLAST
    Transmembranei223 – 24725Helical; Name=5By similarityAdd
    BLAST
    Transmembranei275 – 29622Helical; Name=6By similarityAdd
    BLAST
    Transmembranei312 – 33322Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG317066.
    GeneTreeiENSGT00630000089574.
    HOGENOMiHOG000230486.
    HOVERGENiHBG106919.
    InParanoidiP34975.
    KOiK04214.
    OMAiPFQSTEY.
    OrthoDBiEOG7BKCVQ.
    PhylomeDBiP34975.
    TreeFamiTF315737.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR000452. Kappa_opi_rcpt.
    IPR001418. Opioid_rcpt.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00532. KAPPAOPIOIDR.
    PR00384. OPIOIDR.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34975-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESPIQIFRG EPGPTCAPSA CLLPNSSSWF PNWAESDSNG SVGSEDQQLE    50
    PAHISPAIPV IITAVYSVVF VVGLVGNSLV MFVIIRYTKM KTATNIYIFN 100
    LALADALVTT TMPFQSAVYL MNSWPFGDVL CKIVISIDYY NMFTSIFTLT 150
    MMSVDRYIAV CHPVKALDFR TPLKAKIINI CIWLLASSVG ISAIVLGGTK 200
    VREDVDVIEC SLQFPDDEYS WWDLFMKICV FVFAFVIPVL IIIVCYTLMI 250
    LRLKSVRLLS GSREKDRNLR RITKLVLVVV AVFIICWTPI HIFILVEALG 300
    STSHSTAVLS SYYFCIALGY TNSSLNPVLY AFLDENFKRC FRDFCFPIKM 350
    RMERQSTNRV RNTVQDPASM RDVGGMNKPV 380
    Length:380
    Mass (Da):42,688
    Last modified:February 1, 1994 - v1
    Checksum:i107FEEDB09886F92
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti42 – 421V → L in BAA04109. (PubMed:8103466)Curated
    Sequence conflicti345 – 3451C → Y in AAA41496. (PubMed:8240268)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22001 mRNA. Translation: AAA41495.1.
    D16829 mRNA. Translation: BAA04109.1.
    L22536 mRNA. Translation: AAA41496.1.
    U00442 mRNA. Translation: AAA18261.1.
    D16534 mRNA. Translation: BAA03971.1.
    U17995, U17993, U17994 Genomic DNA. Translation: AAC53249.1.
    PIRiS36143.
    RefSeqiNP_058863.1. NM_017167.2.
    UniGeneiRn.89571.

    Genome annotation databases

    EnsembliENSRNOT00000010254; ENSRNOP00000010255; ENSRNOG00000007647.
    GeneIDi29335.
    KEGGirno:29335.
    UCSCiRGD:69426. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22001 mRNA. Translation: AAA41495.1 .
    D16829 mRNA. Translation: BAA04109.1 .
    L22536 mRNA. Translation: AAA41496.1 .
    U00442 mRNA. Translation: AAA18261.1 .
    D16534 mRNA. Translation: BAA03971.1 .
    U17995 , U17993 , U17994 Genomic DNA. Translation: AAC53249.1 .
    PIRi S36143.
    RefSeqi NP_058863.1. NM_017167.2.
    UniGenei Rn.89571.

    3D structure databases

    ProteinModelPortali P34975.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P34975. 1 interaction.
    STRINGi 10116.ENSRNOP00000010255.

    Chemistry

    BindingDBi P34975.
    ChEMBLi CHEMBL2094129.
    GuidetoPHARMACOLOGYi 318.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P34975.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000010254 ; ENSRNOP00000010255 ; ENSRNOG00000007647 .
    GeneIDi 29335.
    KEGGi rno:29335.
    UCSCi RGD:69426. rat.

    Organism-specific databases

    CTDi 4986.
    RGDi 69426. Oprk1.

    Phylogenomic databases

    eggNOGi NOG317066.
    GeneTreei ENSGT00630000089574.
    HOGENOMi HOG000230486.
    HOVERGENi HBG106919.
    InParanoidi P34975.
    KOi K04214.
    OMAi PFQSTEY.
    OrthoDBi EOG7BKCVQ.
    PhylomeDBi P34975.
    TreeFami TF315737.

    Miscellaneous databases

    NextBioi 608812.
    PROi P34975.

    Gene expression databases

    Genevestigatori P34975.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR000452. Kappa_opi_rcpt.
    IPR001418. Opioid_rcpt.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00532. KAPPAOPIOIDR.
    PR00384. OPIOIDR.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a rat kappa opioid receptor reveals sequence similarities to the mu and delta opioid receptors."
      Chen Y., Mestek A., Liu J., Yu L.
      Biochem. J. 295:625-628(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
      Tissue: Brain.
    2. "Cloning and expression of a cDNA for the rat kappa-opioid receptor."
      Minami M., Toya T., Katao Y., Maekawa K., Nakamura S., Onogi T., Kaneko S., Satoh M.
      FEBS Lett. 329:291-295(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Molecular cloning and expression of a rat kappa opioid receptor."
      Li S., Zhu J., Chen C., Chen Y.-W., Deriel J.K., Ashby B., Liu-Chen L.-Y.
      Biochem. J. 295:629-633(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.
    4. "Cloning and pharmacological characterization of a rat kappa opioid receptor."
      Meng F., Xie G.-X., Thompson R.C., Mansour A., Goldstein A., Watson S.J., Akil H.
      Proc. Natl. Acad. Sci. U.S.A. 90:9954-9958(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.
    5. "cDNA cloning and pharmacological characterization of an opioid receptor with high affinities for kappa-subtype-selective ligands."
      Nishi M., Takeshima H., Fukuda K., Kato S., Mori K.
      FEBS Lett. 330:77-80(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Brain.
    6. "Structure and expression of a rat kappa opioid receptor gene."
      Yakovlev A.G., Krueger K.E., Faden A.I.
      J. Biol. Chem. 270:6421-6424(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
    7. "Role of kappa-opioid receptors in the effects of salvinorin A and ketamine on attention in rats."
      Nemeth C.L., Paine T.A., Rittiner J.E., Beguin C., Carroll F.I., Roth B.L., Cohen B.M., Carlezon W.A. Jr.
      Psychopharmacology 210:263-274(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiOPRK_RAT
    AccessioniPrimary (citable) accession number: P34975
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3