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Protein

Beta-1 adrenergic receptor

Gene

Adrb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Mediates Ras activation through G(s)-alpha- and cAMP-mediated signaling (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei138Agonist or antagonistBy similarity1
Binding sitei143Agonist or antagonistBy similarity1

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-activating adrenergic receptor signaling pathway Source: UniProtKB
  • adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: MGI
  • brown fat cell differentiation Source: MGI
  • cell-cell signaling Source: GO_Central
  • diet induced thermogenesis Source: MGI
  • fear response Source: MGI
  • heat generation Source: MGI
  • negative regulation of multicellular organism growth Source: MGI
  • positive regulation of cAMP biosynthetic process Source: UniProtKB
  • positive regulation of cAMP-mediated signaling Source: UniProtKB
  • positive regulation of GTPase activity Source: UniProtKB
  • positive regulation of heart rate by epinephrine-norepinephrine Source: MGI
  • positive regulation of the force of heart contraction by epinephrine-norepinephrine Source: MGI
  • response to cold Source: MGI
  • vasodilation by norepinephrine-epinephrine involved in regulation of systemic arterial blood pressure Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-390696. Adrenoceptors.
R-MMU-418555. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1 adrenergic receptor
Alternative name(s):
Beta-1 adrenoreceptor
Short name:
Beta-1 adrenoceptor
Gene namesi
Name:Adrb1
Synonyms:Adrb1r
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:87937. Adrb1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 55ExtracellularBy similarityAdd BLAST55
Transmembranei56 – 84Helical; Name=1By similarityAdd BLAST29
Topological domaini85 – 93CytoplasmicBy similarity9
Transmembranei94 – 120Helical; Name=2By similarityAdd BLAST27
Topological domaini121 – 132ExtracellularBy similarityAdd BLAST12
Transmembranei133 – 154Helical; Name=3By similarityAdd BLAST22
Topological domaini155 – 172CytoplasmicBy similarityAdd BLAST18
Transmembranei173 – 196Helical; Name=4By similarityAdd BLAST24
Topological domaini197 – 222ExtracellularBy similarityAdd BLAST26
Transmembranei223 – 248Helical; Name=5By similarityAdd BLAST26
Topological domaini249 – 308CytoplasmicBy similarityAdd BLAST60
Transmembranei309 – 338Helical; Name=6By similarityAdd BLAST30
Topological domaini339 – 343ExtracellularBy similarity5
Transmembranei344 – 366Helical; Name=7By similarityAdd BLAST23
Topological domaini367 – 466CytoplasmicBy similarityAdd BLAST100

GO - Cellular componenti

  • early endosome Source: UniProtKB
  • integral component of plasma membrane Source: GO_Central
  • membrane Source: MGI
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2111395.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000691221 – 466Beta-1 adrenergic receptorAdd BLAST466

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi15N-linked (GlcNAc...)Curated1
Disulfide bondi131 ↔ 216PROSITE-ProRule annotation
Disulfide bondi209 ↔ 215PROSITE-ProRule annotation
Modified residuei296Phosphoserine; by PKASequence analysis1
Modified residuei301Phosphoserine; by PKASequence analysis1
Lipidationi381S-palmitoyl cysteineBy similarity1
Modified residuei401Phosphoserine; by PKASequence analysis1
Modified residuei417PhosphoserineBy similarity1

Post-translational modificationi

Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP34971.
PRIDEiP34971.

PTM databases

iPTMnetiP34971.
PhosphoSitePlusiP34971.

Expressioni

Gene expression databases

BgeeiENSMUSG00000035283.
CleanExiMM_ADRB1.
GenevisibleiP34971. MM.

Interactioni

Subunit structurei

Interacts (via C-terminus PDZ motif) with RAPGEF2; the interaction is direct. Interacts with GOPC, MAGI3 and DLG4 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Pde4dQ010633EBI-7764182,EBI-7764239

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-59567N.
IntActiP34971. 2 interactors.
STRINGi10090.ENSMUSP00000040847.

Structurei

3D structure databases

ProteinModelPortaliP34971.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni218 – 232Agonist and antagonist bindingBy similarityAdd BLAST15
Regioni326 – 333Agonist and antagonist bindingBy similarity8
Regioni352 – 356Agonist and antagonist bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi463 – 466PDZ-BindingBy similarity4

Domaini

The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118774.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP34971.
KOiK04141.
OMAiCVVWAIS.
OrthoDBiEOG091G06VI.
TreeFamiTF316350.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR000507. ADRB1_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00561. ADRENRGCB1AR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34971-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAGALALGA SEPCNLSSAA PLPDGAATAA RLLVLASPPA SLLPPASEGS
60 70 80 90 100
APLSQQWTAG MGLLLALIVL LIVVGNVLVI VAIAKTPRLQ TLTNLFIMSL
110 120 130 140 150
ASADLVMGLL VVPFGATIVV WGRWEYGSFF CELWTSVDVL CVTASIETLC
160 170 180 190 200
VIALDRYLAI TSPFRYQSLL TRARARALVC TVWAISALVS FLPILMHWWR
210 220 230 240 250
AESDEARRCY NDPKCCDFVT NRAYAIASSV VSFYVPLCIM AFVYLRVFRE
260 270 280 290 300
AQKQVKKIDS CERRFLGGPA RPPSPEPSPS PGPPRPADSL ANGRSSKRRP
310 320 330 340 350
SRLVALREQK ALKTLGIIMG VFTLCWLPFF LANVVKAFHR DLVPDRLFVF
360 370 380 390 400
FNWLGYANSA FNPIIYCRSP DFRKAFQRLL CCARRAACRR RAAHGDRPRA
410 420 430 440 450
SGCLARAGPP PSPGAPSDDD DDDAGTTPPA RLLEPWTGCN GGTTTVDSDS
460
SLDEPGRQGF SSESKV
Length:466
Mass (Da):50,494
Last modified:July 27, 2011 - v2
Checksum:iE4861E250B62F735
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65L → V in AAA02929 (PubMed:8395893).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10084 Genomic DNA. Translation: AAA02929.1.
CH466585 Genomic DNA. Translation: EDL01765.1.
BC140970 mRNA. Translation: AAI40971.1.
BC147435 mRNA. Translation: AAI47436.1.
CCDSiCCDS29919.1.
PIRiS36794.
RefSeqiNP_031445.2. NM_007419.2.
UniGeneiMm.46797.

Genome annotation databases

EnsembliENSMUST00000038949; ENSMUSP00000040847; ENSMUSG00000035283.
GeneIDi11554.
KEGGimmu:11554.
UCSCiuc008hze.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10084 Genomic DNA. Translation: AAA02929.1.
CH466585 Genomic DNA. Translation: EDL01765.1.
BC140970 mRNA. Translation: AAI40971.1.
BC147435 mRNA. Translation: AAI47436.1.
CCDSiCCDS29919.1.
PIRiS36794.
RefSeqiNP_031445.2. NM_007419.2.
UniGeneiMm.46797.

3D structure databases

ProteinModelPortaliP34971.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59567N.
IntActiP34971. 2 interactors.
STRINGi10090.ENSMUSP00000040847.

Chemistry databases

ChEMBLiCHEMBL2111395.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP34971.
PhosphoSitePlusiP34971.

Proteomic databases

PaxDbiP34971.
PRIDEiP34971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038949; ENSMUSP00000040847; ENSMUSG00000035283.
GeneIDi11554.
KEGGimmu:11554.
UCSCiuc008hze.2. mouse.

Organism-specific databases

CTDi153.
MGIiMGI:87937. Adrb1.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118774.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP34971.
KOiK04141.
OMAiCVVWAIS.
OrthoDBiEOG091G06VI.
TreeFamiTF316350.

Enzyme and pathway databases

ReactomeiR-MMU-390696. Adrenoceptors.
R-MMU-418555. G alpha (s) signalling events.

Miscellaneous databases

PROiP34971.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035283.
CleanExiMM_ADRB1.
GenevisibleiP34971. MM.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR000507. ADRB1_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00561. ADRENRGCB1AR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADRB1_MOUSE
AccessioniPrimary (citable) accession number: P34971
Secondary accession number(s): B2RVY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.