P34971 (ADRB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1 adrenergic receptor

Alternative name(s):
Beta-1 adrenoreceptor
Short name=Beta-1 adrenoceptor

Gene names

Name:	Adrb1
Synonyms:	Adrb1r

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.
Subunit structure	Interacts with GOPC, MAGI3 and DLG4 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity. Note: Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression By similarity.
Domain	The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor By similarity.
Post-translational modification	Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Rho protein signal transduction Inferred from electronic annotation. Source: Compara adenylate cyclase-activating G-protein coupled receptor signaling pathway Inferred from mutant phenotype PubMed 8693001. Source: MGI aging Inferred from electronic annotation. Source: Compara apoptotic process Inferred from electronic annotation. Source: Compara brown fat cell differentiation Inferred from genetic interaction PubMed 12387862. Source: MGI diet induced thermogenesis Inferred from genetic interaction PubMed 12161655. Source: MGI fear response Inferred from mutant phenotype PubMed 15066288. Source: MGI glycogen catabolic process Inferred from electronic annotation. Source: Compara heat generation Inferred from genetic interaction PubMed 12161655. Source: MGI lipid homeostasis Inferred from electronic annotation. Source: Compara memory Inferred from electronic annotation. Source: Compara negative regulation of multicellular organism growth Inferred from genetic interaction PubMed 12387862. Source: MGI negative regulation of smooth muscle contraction Inferred from electronic annotation. Source: Compara negative regulation of urine volume Inferred from electronic annotation. Source: Compara positive regulation of cAMP biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of cation channel activity Inferred from electronic annotation. Source: Compara positive regulation of cell growth involved in cardiac muscle cell development Inferred from electronic annotation. Source: Compara positive regulation of heart rate by epinephrine-norepinephrine Inferred from mutant phenotype PubMed 10358009PubMed 8693001. Source: MGI positive regulation of saliva secretion Inferred from electronic annotation. Source: Compara positive regulation of systemic arterial blood pressure Inferred from electronic annotation. Source: Compara positive regulation of the force of heart contraction by epinephrine-norepinephrine Inferred from direct assay PubMed 7761854. Source: MGI positive regulation of the force of heart contraction by norepinephrine Inferred from electronic annotation. Source: Compara protein localization to organelle Inferred from electronic annotation. Source: Compara regulation of calcium ion transport Inferred from electronic annotation. Source: Compara regulation of cardiac muscle cell contraction Inferred from electronic annotation. Source: Compara regulation of inhibitory postsynaptic membrane potential Inferred from electronic annotation. Source: Compara response to cold Inferred from genetic interaction PubMed 12161655PubMed 12387862. Source: MGI sensory perception of pain Inferred from electronic annotation. Source: Compara vasodilation by norepinephrine-epinephrine involved in regulation of systemic arterial blood pressure Inferred from mutant phenotype PubMed 10358009PubMed 11641423. Source: MGI wound healing Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from mutant phenotype PubMed 8693001. Source: MGI nucleus Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta1-adrenergic receptor activity Inferred from direct assay PubMed 7738011PubMed 9305915. Source: MGI dopamine binding Inferred from electronic annotation. Source: Compara drug binding Inferred from electronic annotation. Source: Compara epinephrine binding Inferred from electronic annotation. Source: Compara norepinephrine binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Beta-1 adrenergic receptor		PRO_0000069122
Regions
	Topological domain	1 – 55	55	Extracellular By similarity
	Transmembrane	56 – 84	29	Helical; Name=1; By similarity
	Topological domain	85 – 93	9	Cytoplasmic By similarity
	Transmembrane	94 – 120	27	Helical; Name=2; By similarity
	Topological domain	121 – 132	12	Extracellular By similarity
	Transmembrane	133 – 154	22	Helical; Name=3; By similarity
	Topological domain	155 – 172	18	Cytoplasmic By similarity
	Transmembrane	173 – 196	24	Helical; Name=4; By similarity
	Topological domain	197 – 222	26	Extracellular By similarity
	Transmembrane	223 – 248	26	Helical; Name=5; By similarity
	Topological domain	249 – 308	60	Cytoplasmic By similarity
	Transmembrane	309 – 338	30	Helical; Name=6; By similarity
	Topological domain	339 – 343	5	Extracellular By similarity
	Transmembrane	344 – 366	23	Helical; Name=7; By similarity
	Topological domain	367 – 466	100	Cytoplasmic By similarity
	Region	218 – 232	15	Agonist and antagonist binding By similarity
	Region	326 – 333	8	Agonist and antagonist binding By similarity
	Region	352 – 356	5	Agonist and antagonist binding By similarity
	Motif	463 – 466	4	PDZ-Binding By similarity
Sites
	Binding site	138	1	Agonist or antagonist By similarity
	Binding site	143	1	Agonist or antagonist By similarity
Amino acid modifications
	Modified residue	296	1	Phosphoserine; by PKA Potential
	Modified residue	301	1	Phosphoserine; by PKA Potential
	Modified residue	401	1	Phosphoserine; by PKA Potential
	Lipidation	381	1	S-palmitoyl cysteine By similarity
	Glycosylation	15	1	N-linked (GlcNAc...) Probable
	Disulfide bond	131 ↔ 216		By similarity
	Disulfide bond	209 ↔ 215		By similarity
Experimental info
	Sequence conflict	65	1	L → V in AAA02929. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P34971 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: E4861E250B62F735	FASTA	466	50,494
	10 20 30 40 50 60 MGAGALALGA SEPCNLSSAA PLPDGAATAA RLLVLASPPA SLLPPASEGS APLSQQWTAG 70 80 90 100 110 120 MGLLLALIVL LIVVGNVLVI VAIAKTPRLQ TLTNLFIMSL ASADLVMGLL VVPFGATIVV 130 140 150 160 170 180 WGRWEYGSFF CELWTSVDVL CVTASIETLC VIALDRYLAI TSPFRYQSLL TRARARALVC 190 200 210 220 230 240 TVWAISALVS FLPILMHWWR AESDEARRCY NDPKCCDFVT NRAYAIASSV VSFYVPLCIM 250 260 270 280 290 300 AFVYLRVFRE AQKQVKKIDS CERRFLGGPA RPPSPEPSPS PGPPRPADSL ANGRSSKRRP 310 320 330 340 350 360 SRLVALREQK ALKTLGIIMG VFTLCWLPFF LANVVKAFHR DLVPDRLFVF FNWLGYANSA 370 380 390 400 410 420 FNPIIYCRSP DFRKAFQRLL CCARRAACRR RAAHGDRPRA SGCLARAGPP PSPGAPSDDD 430 440 450 460 DDDAGTTPPA RLLEPWTGCN GGTTTVDSDS SLDEPGRQGF SSESKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of the mouse beta 1-adrenergic receptor gene." Jasper J.R., Link R.E., Chruscinski A.J., Kobilka B.K., Bernstein D. Biochim. Biophys. Acta 1178:307-309(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[2]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 303-307, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L10084 Genomic DNA. Translation: AAA02929.1. CH466585 Genomic DNA. Translation: EDL01765.1. BC140970 mRNA. Translation: AAI40971.1. BC147435 mRNA. Translation: AAI47436.1.
IPI	IPI00119827.
PIR	S36794.
RefSeq	NP_031445.2. NM_007419.2.
UniGene	Mm.46797.
3D structure databases
ProteinModelPortal	P34971.
SMR	P34971. Positions 53-380.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-59567N.
STRING	10090.ENSMUSP00000040847.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P34971.
Proteomic databases
PRIDE	P34971.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000038949; ENSMUSP00000040847; ENSMUSG00000035283.
GeneID	11554.
KEGG	mmu:11554.
Organism-specific databases
CTD	153.
MGI	MGI:87937. Adrb1.
Phylogenomic databases
eggNOG	NOG262978.
GeneTree	ENSGT00680000099833.
HOGENOM	HOG000239242.
HOVERGEN	HBG106962.
InParanoid	B2RVY4.
KO	K04141.
OMA	DRPRASG.
OrthoDB	EOG4WQ12W.
Gene expression databases
Bgee	P34971.
CleanEx	MM_ADRB1.
Genevestigator	P34971.
GermOnline	ENSMUSG00000035283. Mus musculus.
Family and domain databases
InterPro	IPR000507. Adrgc_rcpt_B1. IPR002233. Adrnrgc_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01103. ADRENERGICR. PR00561. ADRENRGCB1AR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P34971.
ChEMBL	CHEMBL3440.
NextBio	279050.
SOURCE	Search...

Entry information

Entry name

ADRB1_MOUSE

Accession

Primary (citable) accession number: P34971
Secondary accession number(s): B2RVY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	July 27, 2011
Last modified:	April 3, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program