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P34971

- ADRB1_MOUSE

UniProt

P34971 - ADRB1_MOUSE

Protein

Beta-1 adrenergic receptor

Gene

Adrb1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Mediates Ras activation through G(s)-alpha- and cAMP-mediated signaling By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei138 – 1381Agonist or antagonistBy similarity
    Binding sitei143 – 1431Agonist or antagonistBy similarity

    GO - Molecular functioni

    1. beta1-adrenergic receptor activity Source: MGI
    2. dopamine binding Source: Ensembl
    3. drug binding Source: Ensembl
    4. epinephrine binding Source: Ensembl
    5. norepinephrine binding Source: Ensembl
    6. protein binding Source: IntAct
    7. Ras guanyl-nucleotide exchange factor activity Source: UniProtKB
    8. receptor signaling protein activity Source: UniProtKB

    GO - Biological processi

    1. adenylate cyclase-activating adrenergic receptor signaling pathway Source: UniProtKB
    2. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: MGI
    3. aging Source: Ensembl
    4. apoptotic process Source: Ensembl
    5. brown fat cell differentiation Source: MGI
    6. diet induced thermogenesis Source: MGI
    7. fear response Source: MGI
    8. glycogen catabolic process Source: Ensembl
    9. heat generation Source: MGI
    10. lipid homeostasis Source: Ensembl
    11. memory Source: Ensembl
    12. negative regulation of multicellular organism growth Source: MGI
    13. negative regulation of smooth muscle contraction Source: Ensembl
    14. negative regulation of urine volume Source: Ensembl
    15. positive regulation of apoptotic process Source: Ensembl
    16. positive regulation of cAMP biosynthetic process Source: UniProtKB
    17. positive regulation of cAMP-mediated signaling Source: UniProtKB
    18. positive regulation of cation channel activity Source: Ensembl
    19. positive regulation of cell growth involved in cardiac muscle cell development Source: Ensembl
    20. positive regulation of heart rate by epinephrine-norepinephrine Source: MGI
    21. positive regulation of Ras GTPase activity Source: UniProtKB
    22. positive regulation of renin secretion into blood stream Source: Ensembl
    23. positive regulation of saliva secretion Source: Ensembl
    24. positive regulation of systemic arterial blood pressure Source: Ensembl
    25. positive regulation of the force of heart contraction by epinephrine-norepinephrine Source: MGI
    26. positive regulation of the force of heart contraction by norepinephrine Source: Ensembl
    27. protein localization to organelle Source: Ensembl
    28. regulation of calcium ion transport Source: Ensembl
    29. regulation of cardiac muscle cell contraction Source: Ensembl
    30. regulation of inhibitory postsynaptic membrane potential Source: Ensembl
    31. response to cold Source: MGI
    32. Rho protein signal transduction Source: Ensembl
    33. sensory perception of pain Source: Ensembl
    34. vasodilation by norepinephrine-epinephrine involved in regulation of systemic arterial blood pressure Source: MGI
    35. wound healing Source: Ensembl

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1 adrenergic receptor
    Alternative name(s):
    Beta-1 adrenoreceptor
    Short name:
    Beta-1 adrenoceptor
    Gene namesi
    Name:Adrb1
    Synonyms:Adrb1r
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:87937. Adrb1.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity. Early endosome By similarity
    Note: Colocalizes with RAPGEF2 at the plasma membrane. Found in the Golgi upon GOPC overexpression By similarity.By similarity

    GO - Cellular componenti

    1. early endosome Source: UniProtKB
    2. integral component of membrane Source: UniProtKB-KW
    3. membrane Source: MGI
    4. nucleus Source: Ensembl
    5. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 466466Beta-1 adrenergic receptorPRO_0000069122Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi15 – 151N-linked (GlcNAc...)Curated
    Disulfide bondi131 ↔ 216PROSITE-ProRule annotation
    Disulfide bondi209 ↔ 215PROSITE-ProRule annotation
    Modified residuei296 – 2961Phosphoserine; by PKASequence Analysis
    Modified residuei301 – 3011Phosphoserine; by PKASequence Analysis
    Lipidationi381 – 3811S-palmitoyl cysteineBy similarity
    Modified residuei401 – 4011Phosphoserine; by PKASequence Analysis

    Post-translational modificationi

    Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PRIDEiP34971.

    PTM databases

    PhosphoSiteiP34971.

    Expressioni

    Gene expression databases

    BgeeiP34971.
    CleanExiMM_ADRB1.
    GenevestigatoriP34971.

    Interactioni

    Subunit structurei

    Interacts (via C-terminus PDZ motif) with RAPGEF2; the interaction is direct. Interacts with GOPC, MAGI3 and DLG4 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Pde4dQ010633EBI-7764182,EBI-7764239

    Protein-protein interaction databases

    DIPiDIP-59567N.
    IntActiP34971. 2 interactions.
    STRINGi10090.ENSMUSP00000040847.

    Structurei

    3D structure databases

    ProteinModelPortaliP34971.
    SMRiP34971. Positions 53-380.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5555ExtracellularBy similarityAdd
    BLAST
    Topological domaini85 – 939CytoplasmicBy similarity
    Topological domaini121 – 13212ExtracellularBy similarityAdd
    BLAST
    Topological domaini155 – 17218CytoplasmicBy similarityAdd
    BLAST
    Topological domaini197 – 22226ExtracellularBy similarityAdd
    BLAST
    Topological domaini249 – 30860CytoplasmicBy similarityAdd
    BLAST
    Topological domaini339 – 3435ExtracellularBy similarity
    Topological domaini367 – 466100CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei56 – 8429Helical; Name=1By similarityAdd
    BLAST
    Transmembranei94 – 12027Helical; Name=2By similarityAdd
    BLAST
    Transmembranei133 – 15422Helical; Name=3By similarityAdd
    BLAST
    Transmembranei173 – 19624Helical; Name=4By similarityAdd
    BLAST
    Transmembranei223 – 24826Helical; Name=5By similarityAdd
    BLAST
    Transmembranei309 – 33830Helical; Name=6By similarityAdd
    BLAST
    Transmembranei344 – 36623Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni218 – 23215Agonist and antagonist bindingBy similarityAdd
    BLAST
    Regioni326 – 3338Agonist and antagonist bindingBy similarity
    Regioni352 – 3565Agonist and antagonist bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi463 – 4664PDZ-BindingBy similarity

    Domaini

    The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.By similarity

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG262978.
    GeneTreeiENSGT00720000108411.
    HOGENOMiHOG000239242.
    HOVERGENiHBG106962.
    InParanoidiB2RVY4.
    KOiK04141.
    OMAiCVVWAIS.
    OrthoDBiEOG7BS4BS.
    TreeFamiTF316350.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR002233. ADR_fam.
    IPR000507. ADRB1_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR01103. ADRENERGICR.
    PR00561. ADRENRGCB1AR.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34971-1 [UniParc]FASTAAdd to Basket

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    MGAGALALGA SEPCNLSSAA PLPDGAATAA RLLVLASPPA SLLPPASEGS    50
    APLSQQWTAG MGLLLALIVL LIVVGNVLVI VAIAKTPRLQ TLTNLFIMSL 100
    ASADLVMGLL VVPFGATIVV WGRWEYGSFF CELWTSVDVL CVTASIETLC 150
    VIALDRYLAI TSPFRYQSLL TRARARALVC TVWAISALVS FLPILMHWWR 200
    AESDEARRCY NDPKCCDFVT NRAYAIASSV VSFYVPLCIM AFVYLRVFRE 250
    AQKQVKKIDS CERRFLGGPA RPPSPEPSPS PGPPRPADSL ANGRSSKRRP 300
    SRLVALREQK ALKTLGIIMG VFTLCWLPFF LANVVKAFHR DLVPDRLFVF 350
    FNWLGYANSA FNPIIYCRSP DFRKAFQRLL CCARRAACRR RAAHGDRPRA 400
    SGCLARAGPP PSPGAPSDDD DDDAGTTPPA RLLEPWTGCN GGTTTVDSDS 450
    SLDEPGRQGF SSESKV 466
    Length:466
    Mass (Da):50,494
    Last modified:July 27, 2011 - v2
    Checksum:iE4861E250B62F735
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti65 – 651L → V in AAA02929. (PubMed:8395893)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10084 Genomic DNA. Translation: AAA02929.1.
    CH466585 Genomic DNA. Translation: EDL01765.1.
    BC140970 mRNA. Translation: AAI40971.1.
    BC147435 mRNA. Translation: AAI47436.1.
    CCDSiCCDS29919.1.
    PIRiS36794.
    RefSeqiNP_031445.2. NM_007419.2.
    UniGeneiMm.46797.

    Genome annotation databases

    EnsembliENSMUST00000038949; ENSMUSP00000040847; ENSMUSG00000035283.
    GeneIDi11554.
    KEGGimmu:11554.
    UCSCiuc008hze.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10084 Genomic DNA. Translation: AAA02929.1 .
    CH466585 Genomic DNA. Translation: EDL01765.1 .
    BC140970 mRNA. Translation: AAI40971.1 .
    BC147435 mRNA. Translation: AAI47436.1 .
    CCDSi CCDS29919.1.
    PIRi S36794.
    RefSeqi NP_031445.2. NM_007419.2.
    UniGenei Mm.46797.

    3D structure databases

    ProteinModelPortali P34971.
    SMRi P34971. Positions 53-380.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59567N.
    IntActi P34971. 2 interactions.
    STRINGi 10090.ENSMUSP00000040847.

    Chemistry

    BindingDBi P34971.
    ChEMBLi CHEMBL2111395.
    GuidetoPHARMACOLOGYi 28.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P34971.

    Proteomic databases

    PRIDEi P34971.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000038949 ; ENSMUSP00000040847 ; ENSMUSG00000035283 .
    GeneIDi 11554.
    KEGGi mmu:11554.
    UCSCi uc008hze.2. mouse.

    Organism-specific databases

    CTDi 153.
    MGIi MGI:87937. Adrb1.

    Phylogenomic databases

    eggNOGi NOG262978.
    GeneTreei ENSGT00720000108411.
    HOGENOMi HOG000239242.
    HOVERGENi HBG106962.
    InParanoidi B2RVY4.
    KOi K04141.
    OMAi CVVWAIS.
    OrthoDBi EOG7BS4BS.
    TreeFami TF316350.

    Miscellaneous databases

    NextBioi 279050.
    PROi P34971.
    SOURCEi Search...

    Gene expression databases

    Bgeei P34971.
    CleanExi MM_ADRB1.
    Genevestigatori P34971.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR002233. ADR_fam.
    IPR000507. ADRB1_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01103. ADRENERGICR.
    PR00561. ADRENRGCB1AR.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 303-307, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiADRB1_MOUSE
    AccessioniPrimary (citable) accession number: P34971
    Secondary accession number(s): B2RVY4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3