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P34971

- ADRB1_MOUSE

UniProt

P34971 - ADRB1_MOUSE

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Protein

Beta-1 adrenergic receptor

Gene

Adrb1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Mediates Ras activation through G(s)-alpha- and cAMP-mediated signaling (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei138 – 1381Agonist or antagonistBy similarity
Binding sitei143 – 1431Agonist or antagonistBy similarity

GO - Molecular functioni

  1. beta1-adrenergic receptor activity Source: MGI
  2. dopamine binding Source: Ensembl
  3. drug binding Source: Ensembl
  4. epinephrine binding Source: Ensembl
  5. norepinephrine binding Source: Ensembl
  6. Ras guanyl-nucleotide exchange factor activity Source: UniProtKB
  7. receptor signaling protein activity Source: UniProtKB

GO - Biological processi

  1. adenylate cyclase-activating adrenergic receptor signaling pathway Source: UniProtKB
  2. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: MGI
  3. aging Source: Ensembl
  4. apoptotic process Source: Ensembl
  5. brown fat cell differentiation Source: MGI
  6. diet induced thermogenesis Source: MGI
  7. fear response Source: MGI
  8. glycogen catabolic process Source: Ensembl
  9. heat generation Source: MGI
  10. lipid homeostasis Source: Ensembl
  11. memory Source: Ensembl
  12. negative regulation of multicellular organism growth Source: MGI
  13. negative regulation of smooth muscle contraction Source: Ensembl
  14. negative regulation of urine volume Source: Ensembl
  15. positive regulation of apoptotic process Source: Ensembl
  16. positive regulation of cAMP biosynthetic process Source: UniProtKB
  17. positive regulation of cAMP-mediated signaling Source: UniProtKB
  18. positive regulation of cation channel activity Source: Ensembl
  19. positive regulation of cell growth involved in cardiac muscle cell development Source: Ensembl
  20. positive regulation of heart rate by epinephrine-norepinephrine Source: MGI
  21. positive regulation of Ras GTPase activity Source: UniProtKB
  22. positive regulation of renin secretion into blood stream Source: Ensembl
  23. positive regulation of saliva secretion Source: Ensembl
  24. positive regulation of systemic arterial blood pressure Source: Ensembl
  25. positive regulation of the force of heart contraction by epinephrine-norepinephrine Source: MGI
  26. positive regulation of the force of heart contraction by norepinephrine Source: Ensembl
  27. protein localization to organelle Source: Ensembl
  28. regulation of calcium ion transport Source: Ensembl
  29. regulation of cardiac muscle cell contraction Source: Ensembl
  30. regulation of inhibitory postsynaptic membrane potential Source: Ensembl
  31. response to cold Source: MGI
  32. Rho protein signal transduction Source: Ensembl
  33. sensory perception of pain Source: Ensembl
  34. vasodilation by norepinephrine-epinephrine involved in regulation of systemic arterial blood pressure Source: MGI
  35. wound healing Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1 adrenergic receptor
Alternative name(s):
Beta-1 adrenoreceptor
Short name:
Beta-1 adrenoceptor
Gene namesi
Name:Adrb1
Synonyms:Adrb1r
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:87937. Adrb1.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity. Early endosome By similarity
Note: Colocalizes with RAPGEF2 at the plasma membrane. Found in the Golgi upon GOPC overexpression (By similarity).By similarity

GO - Cellular componenti

  1. early endosome Source: UniProtKB
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: MGI
  4. nucleus Source: Ensembl
  5. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Beta-1 adrenergic receptorPRO_0000069122Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi15 – 151N-linked (GlcNAc...)Curated
Disulfide bondi131 ↔ 216PROSITE-ProRule annotation
Disulfide bondi209 ↔ 215PROSITE-ProRule annotation
Modified residuei296 – 2961Phosphoserine; by PKASequence Analysis
Modified residuei301 – 3011Phosphoserine; by PKASequence Analysis
Lipidationi381 – 3811S-palmitoyl cysteineBy similarity
Modified residuei401 – 4011Phosphoserine; by PKASequence Analysis

Post-translational modificationi

Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiP34971.

PTM databases

PhosphoSiteiP34971.

Expressioni

Gene expression databases

BgeeiP34971.
CleanExiMM_ADRB1.
GenevestigatoriP34971.

Interactioni

Subunit structurei

Interacts (via C-terminus PDZ motif) with RAPGEF2; the interaction is direct. Interacts with GOPC, MAGI3 and DLG4 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Pde4dQ010633EBI-7764182,EBI-7764239

Protein-protein interaction databases

DIPiDIP-59567N.
IntActiP34971. 2 interactions.
STRINGi10090.ENSMUSP00000040847.

Structurei

3D structure databases

ProteinModelPortaliP34971.
SMRiP34971. Positions 53-380.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5555ExtracellularBy similarityAdd
BLAST
Topological domaini85 – 939CytoplasmicBy similarity
Topological domaini121 – 13212ExtracellularBy similarityAdd
BLAST
Topological domaini155 – 17218CytoplasmicBy similarityAdd
BLAST
Topological domaini197 – 22226ExtracellularBy similarityAdd
BLAST
Topological domaini249 – 30860CytoplasmicBy similarityAdd
BLAST
Topological domaini339 – 3435ExtracellularBy similarity
Topological domaini367 – 466100CytoplasmicBy similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei56 – 8429Helical; Name=1By similarityAdd
BLAST
Transmembranei94 – 12027Helical; Name=2By similarityAdd
BLAST
Transmembranei133 – 15422Helical; Name=3By similarityAdd
BLAST
Transmembranei173 – 19624Helical; Name=4By similarityAdd
BLAST
Transmembranei223 – 24826Helical; Name=5By similarityAdd
BLAST
Transmembranei309 – 33830Helical; Name=6By similarityAdd
BLAST
Transmembranei344 – 36623Helical; Name=7By similarityAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni218 – 23215Agonist and antagonist bindingBy similarityAdd
BLAST
Regioni326 – 3338Agonist and antagonist bindingBy similarity
Regioni352 – 3565Agonist and antagonist bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi463 – 4664PDZ-BindingBy similarity

Domaini

The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG262978.
GeneTreeiENSGT00760000118774.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP34971.
KOiK04141.
OMAiCVVWAIS.
OrthoDBiEOG7BS4BS.
TreeFamiTF316350.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR002233. ADR_fam.
IPR000507. ADRB1_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00561. ADRENRGCB1AR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34971-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGAGALALGA SEPCNLSSAA PLPDGAATAA RLLVLASPPA SLLPPASEGS
60 70 80 90 100
APLSQQWTAG MGLLLALIVL LIVVGNVLVI VAIAKTPRLQ TLTNLFIMSL
110 120 130 140 150
ASADLVMGLL VVPFGATIVV WGRWEYGSFF CELWTSVDVL CVTASIETLC
160 170 180 190 200
VIALDRYLAI TSPFRYQSLL TRARARALVC TVWAISALVS FLPILMHWWR
210 220 230 240 250
AESDEARRCY NDPKCCDFVT NRAYAIASSV VSFYVPLCIM AFVYLRVFRE
260 270 280 290 300
AQKQVKKIDS CERRFLGGPA RPPSPEPSPS PGPPRPADSL ANGRSSKRRP
310 320 330 340 350
SRLVALREQK ALKTLGIIMG VFTLCWLPFF LANVVKAFHR DLVPDRLFVF
360 370 380 390 400
FNWLGYANSA FNPIIYCRSP DFRKAFQRLL CCARRAACRR RAAHGDRPRA
410 420 430 440 450
SGCLARAGPP PSPGAPSDDD DDDAGTTPPA RLLEPWTGCN GGTTTVDSDS
460
SLDEPGRQGF SSESKV
Length:466
Mass (Da):50,494
Last modified:July 27, 2011 - v2
Checksum:iE4861E250B62F735
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651L → V in AAA02929. (PubMed:8395893)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10084 Genomic DNA. Translation: AAA02929.1.
CH466585 Genomic DNA. Translation: EDL01765.1.
BC140970 mRNA. Translation: AAI40971.1.
BC147435 mRNA. Translation: AAI47436.1.
CCDSiCCDS29919.1.
PIRiS36794.
RefSeqiNP_031445.2. NM_007419.2.
UniGeneiMm.46797.

Genome annotation databases

EnsembliENSMUST00000038949; ENSMUSP00000040847; ENSMUSG00000035283.
GeneIDi11554.
KEGGimmu:11554.
UCSCiuc008hze.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10084 Genomic DNA. Translation: AAA02929.1 .
CH466585 Genomic DNA. Translation: EDL01765.1 .
BC140970 mRNA. Translation: AAI40971.1 .
BC147435 mRNA. Translation: AAI47436.1 .
CCDSi CCDS29919.1.
PIRi S36794.
RefSeqi NP_031445.2. NM_007419.2.
UniGenei Mm.46797.

3D structure databases

ProteinModelPortali P34971.
SMRi P34971. Positions 53-380.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59567N.
IntActi P34971. 2 interactions.
STRINGi 10090.ENSMUSP00000040847.

Chemistry

BindingDBi P34971.
ChEMBLi CHEMBL2111395.
GuidetoPHARMACOLOGYi 28.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P34971.

Proteomic databases

PRIDEi P34971.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000038949 ; ENSMUSP00000040847 ; ENSMUSG00000035283 .
GeneIDi 11554.
KEGGi mmu:11554.
UCSCi uc008hze.2. mouse.

Organism-specific databases

CTDi 153.
MGIi MGI:87937. Adrb1.

Phylogenomic databases

eggNOGi NOG262978.
GeneTreei ENSGT00760000118774.
HOGENOMi HOG000239242.
HOVERGENi HBG106962.
InParanoidi P34971.
KOi K04141.
OMAi CVVWAIS.
OrthoDBi EOG7BS4BS.
TreeFami TF316350.

Miscellaneous databases

NextBioi 279050.
PROi P34971.
SOURCEi Search...

Gene expression databases

Bgeei P34971.
CleanExi MM_ADRB1.
Genevestigatori P34971.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR002233. ADR_fam.
IPR000507. ADRB1_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR01103. ADRENERGICR.
PR00561. ADRENRGCB1AR.
PR00237. GPCRRHODOPSN.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 303-307, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.

Entry informationi

Entry nameiADRB1_MOUSE
AccessioniPrimary (citable) accession number: P34971
Secondary accession number(s): B2RVY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3