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P34971 (ADRB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1 adrenergic receptor
Alternative name(s):
Beta-1 adrenoreceptor
Short name=Beta-1 adrenoceptor
Gene names
Name:Adrb1
Synonyms:Adrb1r
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Mediates Ras activation through G(s)-alpha- and cAMP-mediated signaling By similarity.

Subunit structure

Interacts (via C-terminus PDZ motif) with RAPGEF2; the interaction is direct. Interacts with GOPC, MAGI3 and DLG4 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Early endosome By similarity. Note: Colocalizes with RAPGEF2 at the plasma membrane. Found in the Golgi upon GOPC overexpression By similarity.

Domain

The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor By similarity.

Post-translational modification

Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.

Ontologies

Keywords
   Cellular componentCell membrane
Endosome
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processRho protein signal transduction

Inferred from electronic annotation. Source: Ensembl

adenylate cyclase-activating G-protein coupled receptor signaling pathway

Inferred from mutant phenotype PubMed 8693001. Source: MGI

adenylate cyclase-activating adrenergic receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

aging

Inferred from electronic annotation. Source: Ensembl

apoptotic process

Inferred from electronic annotation. Source: Ensembl

brown fat cell differentiation

Inferred from genetic interaction PubMed 12387862. Source: MGI

diet induced thermogenesis

Inferred from genetic interaction PubMed 12161655. Source: MGI

fear response

Inferred from mutant phenotype PubMed 15066288. Source: MGI

glycogen catabolic process

Inferred from electronic annotation. Source: Ensembl

heat generation

Inferred from genetic interaction PubMed 12161655. Source: MGI

lipid homeostasis

Inferred from electronic annotation. Source: Ensembl

memory

Inferred from electronic annotation. Source: Ensembl

negative regulation of multicellular organism growth

Inferred from genetic interaction PubMed 12387862. Source: MGI

negative regulation of smooth muscle contraction

Inferred from electronic annotation. Source: Ensembl

negative regulation of urine volume

Inferred from electronic annotation. Source: Ensembl

positive regulation of Ras GTPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of cAMP biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cAMP-mediated signaling

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cation channel activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell growth involved in cardiac muscle cell development

Inferred from electronic annotation. Source: Ensembl

positive regulation of heart rate by epinephrine-norepinephrine

Inferred from mutant phenotype PubMed 10358009PubMed 8693001. Source: MGI

positive regulation of renin secretion into blood stream

Inferred from electronic annotation. Source: Ensembl

positive regulation of saliva secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of systemic arterial blood pressure

Inferred from electronic annotation. Source: Ensembl

positive regulation of the force of heart contraction by epinephrine-norepinephrine

Inferred from direct assay PubMed 7761854. Source: MGI

positive regulation of the force of heart contraction by norepinephrine

Inferred from electronic annotation. Source: Ensembl

protein localization to organelle

Inferred from electronic annotation. Source: Ensembl

regulation of calcium ion transport

Inferred from electronic annotation. Source: Ensembl

regulation of cardiac muscle cell contraction

Inferred from electronic annotation. Source: Ensembl

regulation of inhibitory postsynaptic membrane potential

Inferred from electronic annotation. Source: Ensembl

response to cold

Inferred from genetic interaction PubMed 12161655PubMed 12387862. Source: MGI

sensory perception of pain

Inferred from electronic annotation. Source: Ensembl

vasodilation by norepinephrine-epinephrine involved in regulation of systemic arterial blood pressure

Inferred from mutant phenotype PubMed 10358009PubMed 11641423. Source: MGI

wound healing

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentearly endosome

Inferred from sequence or structural similarity. Source: UniProtKB

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Inferred from mutant phenotype PubMed 8693001. Source: MGI

nucleus

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionRas guanyl-nucleotide exchange factor activity

Inferred from sequence or structural similarity. Source: UniProtKB

beta1-adrenergic receptor activity

Inferred from direct assay PubMed 7738011PubMed 9305915. Source: MGI

dopamine binding

Inferred from electronic annotation. Source: Ensembl

drug binding

Inferred from electronic annotation. Source: Ensembl

epinephrine binding

Inferred from electronic annotation. Source: Ensembl

norepinephrine binding

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 18188154. Source: IntAct

receptor signaling protein activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Pde4dQ010633EBI-7764182,EBI-7764239

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 466466Beta-1 adrenergic receptor
PRO_0000069122

Regions

Topological domain1 – 5555Extracellular By similarity
Transmembrane56 – 8429Helical; Name=1; By similarity
Topological domain85 – 939Cytoplasmic By similarity
Transmembrane94 – 12027Helical; Name=2; By similarity
Topological domain121 – 13212Extracellular By similarity
Transmembrane133 – 15422Helical; Name=3; By similarity
Topological domain155 – 17218Cytoplasmic By similarity
Transmembrane173 – 19624Helical; Name=4; By similarity
Topological domain197 – 22226Extracellular By similarity
Transmembrane223 – 24826Helical; Name=5; By similarity
Topological domain249 – 30860Cytoplasmic By similarity
Transmembrane309 – 33830Helical; Name=6; By similarity
Topological domain339 – 3435Extracellular By similarity
Transmembrane344 – 36623Helical; Name=7; By similarity
Topological domain367 – 466100Cytoplasmic By similarity
Region218 – 23215Agonist and antagonist binding By similarity
Region326 – 3338Agonist and antagonist binding By similarity
Region352 – 3565Agonist and antagonist binding By similarity
Motif463 – 4664PDZ-Binding By similarity

Sites

Binding site1381Agonist or antagonist By similarity
Binding site1431Agonist or antagonist By similarity

Amino acid modifications

Modified residue2961Phosphoserine; by PKA Potential
Modified residue3011Phosphoserine; by PKA Potential
Modified residue4011Phosphoserine; by PKA Potential
Lipidation3811S-palmitoyl cysteine By similarity
Glycosylation151N-linked (GlcNAc...) Probable
Disulfide bond131 ↔ 216 By similarity
Disulfide bond209 ↔ 215 By similarity

Experimental info

Sequence conflict651L → V in AAA02929. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P34971 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: E4861E250B62F735

FASTA46650,494
        10         20         30         40         50         60 
MGAGALALGA SEPCNLSSAA PLPDGAATAA RLLVLASPPA SLLPPASEGS APLSQQWTAG 

        70         80         90        100        110        120 
MGLLLALIVL LIVVGNVLVI VAIAKTPRLQ TLTNLFIMSL ASADLVMGLL VVPFGATIVV 

       130        140        150        160        170        180 
WGRWEYGSFF CELWTSVDVL CVTASIETLC VIALDRYLAI TSPFRYQSLL TRARARALVC 

       190        200        210        220        230        240 
TVWAISALVS FLPILMHWWR AESDEARRCY NDPKCCDFVT NRAYAIASSV VSFYVPLCIM 

       250        260        270        280        290        300 
AFVYLRVFRE AQKQVKKIDS CERRFLGGPA RPPSPEPSPS PGPPRPADSL ANGRSSKRRP 

       310        320        330        340        350        360 
SRLVALREQK ALKTLGIIMG VFTLCWLPFF LANVVKAFHR DLVPDRLFVF FNWLGYANSA 

       370        380        390        400        410        420 
FNPIIYCRSP DFRKAFQRLL CCARRAACRR RAAHGDRPRA SGCLARAGPP PSPGAPSDDD 

       430        440        450        460 
DDDAGTTPPA RLLEPWTGCN GGTTTVDSDS SLDEPGRQGF SSESKV 

« Hide

References

« Hide 'large scale' references
[1]"Primary structure of the mouse beta 1-adrenergic receptor gene."
Jasper J.R., Link R.E., Chruscinski A.J., Kobilka B.K., Bernstein D.
Biochim. Biophys. Acta 1178:307-309(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 303-307, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L10084 Genomic DNA. Translation: AAA02929.1.
CH466585 Genomic DNA. Translation: EDL01765.1.
BC140970 mRNA. Translation: AAI40971.1.
BC147435 mRNA. Translation: AAI47436.1.
CCDSCCDS29919.1.
PIRS36794.
RefSeqNP_031445.2. NM_007419.2.
UniGeneMm.46797.

3D structure databases

ProteinModelPortalP34971.
SMRP34971. Positions 53-380.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59567N.
IntActP34971. 2 interactions.
STRING10090.ENSMUSP00000040847.

Chemistry

BindingDBP34971.
ChEMBLCHEMBL2111395.
GuidetoPHARMACOLOGY28.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP34971.

Proteomic databases

PRIDEP34971.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000038949; ENSMUSP00000040847; ENSMUSG00000035283.
GeneID11554.
KEGGmmu:11554.
UCSCuc008hze.2. mouse.

Organism-specific databases

CTD153.
MGIMGI:87937. Adrb1.

Phylogenomic databases

eggNOGNOG262978.
GeneTreeENSGT00720000108411.
HOGENOMHOG000239242.
HOVERGENHBG106962.
InParanoidB2RVY4.
KOK04141.
OMACVVWAIS.
OrthoDBEOG7BS4BS.
TreeFamTF316350.

Gene expression databases

BgeeP34971.
CleanExMM_ADRB1.
GenevestigatorP34971.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR002233. ADR_fam.
IPR000507. ADRB1_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR01103. ADRENERGICR.
PR00561. ADRENRGCB1AR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279050.
PROP34971.
SOURCESearch...

Entry information

Entry nameADRB1_MOUSE
AccessionPrimary (citable) accession number: P34971
Secondary accession number(s): B2RVY4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries