P34969 (5HT7R_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-hydroxytryptamine receptor 7
Short name=5-HT-7
Short name=5-HT7

Alternative name(s):
5-HT-X
Serotonin receptor 7

Gene names

Name:

HTR7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Isoform A is the predominant isoform in spleen, caudate and hippocampus. Isoform B is expressed at lower levels. Isoform D is a minor isoform in term of expression. Ref.1
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Traceable author statement Ref.2. Source: ProtInc blood circulation Traceable author statement Ref.2. Source: ProtInc circadian rhythm Traceable author statement PubMed 8398139. Source: ProtInc synaptic transmission Traceable author statement Ref.2. Source: ProtInc
Cellular_component	integral to plasma membrane Traceable author statement Ref.2. Source: ProtInc
Molecular_function	serotonin receptor activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Isoform A and isoform B appear to be expressed at higher levels.

Isoform D (identifier: P34969-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: P34969-2)

The sequence of this isoform differs from the canonical sequence as follows:
     433-479: RACTRRVLLRPEKRPPVSVWVLQSPDHHNWLADKMLTTVEKKVMIHD → QNADYCRKKGHDS

Isoform B (identifier: P34969-3)

The sequence of this isoform differs from the canonical sequence as follows:
     433-479: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	5-hydroxytryptamine receptor 7		PRO_0000068979
Regions
	Topological domain	1 – 83	83	Extracellular By similarity
	Transmembrane	84 – 104	21	Helical; Name=1; By similarity
	Topological domain	105 – 117	13	Cytoplasmic By similarity
	Transmembrane	118 – 138	21	Helical; Name=2; By similarity
	Topological domain	139 – 157	19	Extracellular By similarity
	Transmembrane	158 – 178	21	Helical; Name=3; By similarity
	Topological domain	179 – 201	23	Cytoplasmic By similarity
	Transmembrane	202 – 222	21	Helical; Name=4; By similarity
	Topological domain	223 – 236	14	Extracellular By similarity
	Transmembrane	237 – 257	21	Helical; Name=5; By similarity
	Topological domain	258 – 325	68	Cytoplasmic By similarity
	Transmembrane	326 – 346	21	Helical; Name=6; By similarity
	Topological domain	347 – 367	21	Extracellular By similarity
	Transmembrane	368 – 388	21	Helical; Name=7; By similarity
	Topological domain	389 – 479	91	Cytoplasmic By similarity
Amino acid modifications
	Lipidation	401	1	S-palmitoyl cysteine Potential
	Glycosylation	5	1	N-linked (GlcNAc...) Ref.9
	Glycosylation	66	1	N-linked (GlcNAc...) Ref.9
	Disulfide bond	155 ↔ 231		By similarity
Natural variations
	Alternative sequence	433 – 479	47	RACTR…VMIHD → QNADYCRKKGHDS in isoform A.		VSP_001857
	Alternative sequence	433 – 479	47	Missing in isoform B.		VSP_001856
	Natural variant	92	1	T → K. Ref.10		VAR_012995
	Natural variant	279	1	P → L. Ref.10		VAR_012996
	Natural variant	448	1	P → Q. Corresponds to variant rs33954285 [ dbSNP | Ensembl ].		VAR_049365

Sequences

	Sequence		Length	Mass (Da)
	Isoform D [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 1F62E985EADE1F23	FASTA	479	53,555
	10 20 30 40 50 60 MMDVNSSGRP DLYGHLRSFL LPEVGRGLPD LSPDGGADPV AGSWAPHLLS EVTASPAPTW 70 80 90 100 110 120 DAPPDNASGC GEQINYGRVE KVVIGSILTL ITLLTIAGNC LVVISVCFVK KLRQPSNYLI 130 140 150 160 170 180 VSLALADLSV AVAVMPFVSV TDLIGGKWIF GHFFCNVFIA MDVMCCTASI MTLCVISIDR 190 200 210 220 230 240 YLGITRPLTY PVRQNGKCMA KMILSVWLLS ASITLPPLFG WAQNVNDDKV CLISQDFGYT 250 260 270 280 290 300 IYSTAVAFYI PMSVMLFMYY QIYKAARKSA AKHKFPGFPR VEPDSVIALN GIVKLQKEVE 310 320 330 340 350 360 ECANLSRLLK HERKNISIFK REQKAATTLG IIVGAFTVCW LPFFLLSTAR PFICGTSCSC 370 380 390 400 410 420 IPLWVERTFL WLGYANSLIN PFIYAFFNRD LRTTYRSLLQ CQYRNINRKL SAAGMHEALK 430 440 450 460 470 LAERPERPEF VLRACTRRVL LRPEKRPPVS VWVLQSPDHH NWLADKMLTT VEKKVMIHD
	Isoform A [UniParc]. Checksum: A09BE5E3CBCAB232	FASTA	445	49,488
	10 20 30 40 50 60 MMDVNSSGRP DLYGHLRSFL LPEVGRGLPD LSPDGGADPV AGSWAPHLLS EVTASPAPTW 70 80 90 100 110 120 DAPPDNASGC GEQINYGRVE KVVIGSILTL ITLLTIAGNC LVVISVCFVK KLRQPSNYLI 130 140 150 160 170 180 VSLALADLSV AVAVMPFVSV TDLIGGKWIF GHFFCNVFIA MDVMCCTASI MTLCVISIDR 190 200 210 220 230 240 YLGITRPLTY PVRQNGKCMA KMILSVWLLS ASITLPPLFG WAQNVNDDKV CLISQDFGYT 250 260 270 280 290 300 IYSTAVAFYI PMSVMLFMYY QIYKAARKSA AKHKFPGFPR VEPDSVIALN GIVKLQKEVE 310 320 330 340 350 360 ECANLSRLLK HERKNISIFK REQKAATTLG IIVGAFTVCW LPFFLLSTAR PFICGTSCSC 370 380 390 400 410 420 IPLWVERTFL WLGYANSLIN PFIYAFFNRD LRTTYRSLLQ CQYRNINRKL SAAGMHEALK 430 440 LAERPERPEF VLQNADYCRK KGHDS
	Isoform B [UniParc]. Checksum: D6202D8C921D627E	FASTA	432	47,984
	10 20 30 40 50 60 MMDVNSSGRP DLYGHLRSFL LPEVGRGLPD LSPDGGADPV AGSWAPHLLS EVTASPAPTW 70 80 90 100 110 120 DAPPDNASGC GEQINYGRVE KVVIGSILTL ITLLTIAGNC LVVISVCFVK KLRQPSNYLI 130 140 150 160 170 180 VSLALADLSV AVAVMPFVSV TDLIGGKWIF GHFFCNVFIA MDVMCCTASI MTLCVISIDR 190 200 210 220 230 240 YLGITRPLTY PVRQNGKCMA KMILSVWLLS ASITLPPLFG WAQNVNDDKV CLISQDFGYT 250 260 270 280 290 300 IYSTAVAFYI PMSVMLFMYY QIYKAARKSA AKHKFPGFPR VEPDSVIALN GIVKLQKEVE 310 320 330 340 350 360 ECANLSRLLK HERKNISIFK REQKAATTLG IIVGAFTVCW LPFFLLSTAR PFICGTSCSC 370 380 390 400 410 420 IPLWVERTFL WLGYANSLIN PFIYAFFNRD LRTTYRSLLQ CQYRNINRKL SAAGMHEALK 430 LAERPERPEF VL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Four 5-hydroxytryptamine7 (5-HT7) receptor isoforms in human and rat produced by alternative splicing: species differences due to altered intron-exon organization." Heidmann D.E.A., Metcalf M.A., Kohen R., Hamblin M.W. J. Neurochem. 68:1372-1381(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
[2]	"Cloning of a novel human serotonin receptor (5-HT7) positively linked to adenylate cyclase." Bard J.A., Zgombick J.M., Adham N., Vaysse P., Branchek T.A., Weinshank R.L. J. Biol. Chem. 268:23422-23426(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Tissue: Fetal brain and Placenta.
[3]	"Isolation of cDNA coding for 5-hydroxytryptamine receptor 7, transcript variant b (HTR7B)." King M.M., Aronstam R.S., Sharma S.V. Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Tissue: Brain.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[5]	"Human protein factory for converting the transcriptome into an in vitro-expressed proteome." Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B. , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N. Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
[6]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Tissue: Testis.
[9]	"Biochemical and pharmacological study of N-linked glycosylation of the human serotonin 5-HT(7)a receptor." Gellynck E., Andressen K.W., Lintermans B., Haegeman G., Levy F.O., Vanhoenacker P., Van Craenenbroeck K. FEBS J. 279:1994-2003(2012) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-5 AND ASN-66.
[10]	"The human serotonin 7 (5-HT7) receptor gene: genomic organization and systematic mutation screening in schizophrenia and bipolar affective disorder." Erdmann J., Nothen M.M., Shimron-Abarbanell D., Rietschel M., Albus M., Borrmann M., Maier W., Franzek E., Korner J., Weigelt B., Fimmers R., Propping P. Mol. Psychiatry 1:392-397(1996) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS LYS-92 AND LEU-279.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U68487 mRNA. Translation: AAB48393.1. U68488 mRNA. Translation: AAB48394.1. U68492 Genomic DNA. Translation: AAF07218.1. U68493, U68492 Genomic DNA. Translation: AAF07217.1. U68493, U68492 Genomic DNA. Translation: AAB48397.2. L21195 mRNA. Translation: AAC37538.1. AY493988 mRNA. Translation: AAR87480.1. AK292606 mRNA. Translation: BAF85295.1. AB451482 mRNA. Translation: BAG70296.1. AL360011 Genomic DNA. Translation: CAH69966.1. AL360011 Genomic DNA. Translation: CAH69967.1. AL360011 Genomic DNA. Translation: CAH69968.1. CH471066 Genomic DNA. Translation: EAW50118.1. CH471066 Genomic DNA. Translation: EAW50119.1. CH471066 Genomic DNA. Translation: EAW50120.1. BC047526 mRNA. Translation: AAH47526.1.
IPI	IPI00003048. IPI00015395. IPI00553192.
PIR	A48881.
RefSeq	NP_000863.1. NM_000872.4. NP_062873.1. NM_019859.3. NP_062874.1. NM_019860.3.
UniGene	Hs.73739.
3D structure databases
ProteinModelPortal	P34969.
ModBase	Search...
Protein-protein interaction databases
IntAct	P34969. 2 interactions.
STRING	9606.ENSP00000337949.
Protein family/group databases
GPCRDB	Search...
Polymorphism databases
DMDM	8488960.
Proteomic databases
PaxDb	P34969.
PRIDE	P34969.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000277874; ENSP00000277874; ENSG00000148680. ENST00000336152; ENSP00000337949; ENSG00000148680. ENST00000371719; ENSP00000360784; ENSG00000148680.
GeneID	3363.
KEGG	hsa:3363.
UCSC	uc001kgz.3. human. uc001kha.3. human.
Organism-specific databases
CTD	3363.
GeneCards	GC10M092490.
HGNC	HGNC:5302. HTR7.
HPA	CAB022708.
MIM	182137. gene.
neXtProt	NX_P34969.
PharmGKB	PA29561.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG249628.
HOVERGEN	HBG106962.
InParanoid	P34969.
KO	K04163.
OMA	PTWDAPP.
OrthoDB	EOG4F1X37.
PhylomeDB	P34969.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	P34969.
Bgee	P34969.
CleanEx	HS_HTR7.
Genevestigator	P34969.
GermOnline	ENSG00000148680. Homo sapiens.
Family and domain databases
InterPro	IPR001069. 5HT_7_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00652. 5HT7RECEPTR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P34969.
ChEMBL	CHEMBL3155.
DrugBank	DB00216. Eletriptan. DB00247. Methysergide. DB00246. Ziprasidone.
GenomeRNAi	3363.
NextBio	13312.
SOURCE	Search...

Entry information

Entry name

5HT7R_HUMAN

Accession

Primary (citable) accession number: P34969
Secondary accession number(s): B5BUP6 , P78336, P78372, P78516, Q5VX01, Q5VX02, Q5VX03

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	May 30, 2000
Last modified:	May 1, 2013
This is version 127 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.