Reviewed, UniProtKB/Swiss-Prot P34967 (RIP2_PHYDI)

Names and origin

Protein names	Recommended name:     Protein synthesis inhibitor PD-S2     EC=3.2.2.22 Alternative name(s):     Ribosome-inactivating protein PD-S2     rRNA N-glycosidase
Organism	Phytolacca dioica (Bella sombra tree) (Phytolacca arborea)
Taxonomic identifier	29725 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Phytolaccaceae › Phytolacca

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits protein synthesis in animal cells. Useful as immunotoxin for pharmacological applications.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Tissue specificity	Seeds.
Post-translational modification	Glycosylated. Ref.1
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Protein synthesis inhibitor PD-S2		PRO_0000221406
Amino acid modifications
	Glycosylation	120	1	N-linked (GlcNAc...) Ref.1
	Disulfide bond	34 ↔ 262		By similarity
	Disulfide bond	88 ↔ 110		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34967-1 [UniParc]. Last modified May 10, 2004. Version 2. Checksum: EAD68733A166DCA3	FASTA	265	29,587
	10 20 30 40 50 60 VSTITFDVGS ATISKYTTFL ESLRNQAKDP SLKCYGIPML PNTNPNPKYL LVELNAKLSS 70 80 90 100 110 120 GEVKSITLML RRHNLYVMGY SDPYDNKCRY HIFKDISSTT ERKDVETTLC PNASSRVSKN 130 140 150 160 170 180 ISYDSSYPAL ENKAGRSRSQ VQLGIQILNS DIGKISGVKS FTDKTEAEFL LVAIQMVSEA 190 200 210 220 230 240 TRFKYIENQV KTNFNRAFYP NAKVLNLEET WGKISMAIHG AKNGAFSKPL ELQNADGSKW 250 260 IVLRVDEIKP DVALLKYVSG SCQAT

References

[1]	"Complete amino-acid sequence of PD-S2, a new ribosome-inactivating protein from seeds of Phytolacca dioica L." Del Vecchio Blanco F., Bolognesi A., Malorni A., Sande M.J.W., Savino G., Parente A. Biochim. Biophys. Acta 1338:137-144(1997) [PubMed: 9074624] [Abstract] Cited for: PROTEIN SEQUENCE, GLYCOSYLATION AT ASN-120. Tissue: Seed.
[2]	"Purification and partial characterization of single-chain ribosome-inactivating proteins from the seeds of Phytolacca dioica L." Parente A., de Luca P., Bolognesi A., Barbieri L., Battelli M.G., Abbondanza A., Sande M.J.W., Gigliano G.S., Tazzari P.L., Stirpe F. Biochim. Biophys. Acta 1216:43-49(1993) [PubMed: 8218414] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-32. Tissue: Seed.

Cross-references

Sequence databases
PIR	S38528.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 295871.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR016139. Ribosome_inactivat_prot_sub2. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit. G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP2_PHYDI

Accession

Primary (citable) accession number: P34967

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	May 10, 2004
Last modified:	January 19, 2010
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)