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P34948

- MPI_CANAL

UniProt

P34948 - MPI_CANAL

Protein

Mannose-6-phosphate isomerase

Gene

PMI1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

    Catalytic activityi

    D-mannose 6-phosphate = D-fructose 6-phosphate.

    Cofactori

    Binds 1 zinc ion per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi111 – 1111Zinc
    Metal bindingi113 – 1131Zinc
    Metal bindingi138 – 1381Zinc
    Sitei150 – 1501Its modification inactivates the enzyme
    Metal bindingi285 – 2851Zinc
    Active sitei304 – 30411 Publication

    GO - Molecular functioni

    1. mannose-6-phosphate isomerase activity Source: CGD
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. fungal-type cell wall biogenesis Source: CGD
    2. fungal-type cell wall organization Source: CGD
    3. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
    4. protein glycosylation Source: CGD

    Keywords - Molecular functioni

    Isomerase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00126; UER00423.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannose-6-phosphate isomerase (EC:5.3.1.8)
    Alternative name(s):
    Phosphohexomutase
    Phosphomannose isomerase
    Short name:
    PMI
    Gene namesi
    Name:PMI1
    Synonyms:MANA
    ORF Names:CaO19.1390
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
    ProteomesiUP000000559: Unassembled WGS sequence

    Organism-specific databases

    CGDiCAL0003967. PMI1.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedCurated
    Chaini2 – 441440Mannose-6-phosphate isomerasePRO_0000194241Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi5476.CAL0003967.

    Structurei

    Secondary structure

    1
    441
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 118
    Beta strandi19 – 213
    Helixi22 – 243
    Helixi26 – 349
    Beta strandi44 – 463
    Beta strandi48 – 525
    Beta strandi59 – 613
    Turni62 – 665
    Helixi69 – 757
    Helixi77 – 804
    Helixi83 – 897
    Beta strandi92 – 943
    Beta strandi96 – 10611
    Beta strandi110 – 1123
    Helixi116 – 12510
    Turni127 – 1293
    Beta strandi138 – 1447
    Beta strandi146 – 1527
    Helixi155 – 16410
    Helixi166 – 1727
    Helixi174 – 18310
    Helixi193 – 21018
    Helixi214 – 23017
    Helixi232 – 2365
    Helixi242 – 25211
    Helixi258 – 2614
    Turni262 – 2654
    Beta strandi266 – 2716
    Beta strandi276 – 2794
    Beta strandi285 – 29814
    Beta strandi303 – 3075
    Helixi314 – 3207
    Helixi328 – 3314
    Beta strandi341 – 3444
    Beta strandi346 – 3516
    Beta strandi354 – 3574
    Beta strandi359 – 3646
    Turni367 – 3693
    Beta strandi372 – 3754
    Beta strandi382 – 39514
    Beta strandi398 – 4069
    Beta strandi410 – 4134
    Beta strandi419 – 4235
    Beta strandi433 – 4386

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PMIX-ray1.70A2-441[»]
    ProteinModelPortaliP34948.
    SMRiP34948. Positions 2-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP34948.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1482.
    KOiK01809.
    OrthoDBiEOG7WDNCR.

    Family and domain databases

    Gene3Di2.60.120.10. 3 hits.
    InterProiIPR001250. Man6P_Isoase-1.
    IPR016305. Mannose-6-P_Isomerase.
    IPR018050. Pmannose_isomerase-type1_CS.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view]
    PANTHERiPTHR10309. PTHR10309. 1 hit.
    PfamiPF01238. PMI_typeI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
    PRINTSiPR00714. MAN6PISMRASE.
    SUPFAMiSSF51182. SSF51182. 1 hit.
    TIGRFAMsiTIGR00218. manA. 1 hit.
    PROSITEiPS00965. PMI_I_1. 1 hit.
    PS00966. PMI_I_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P34948-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSEKLFRIQ CGYQNYDWGK IGSSSAVAQF VHNSDPSITI DETKPYAELW    50
    MGTHPSVPSK AIDLNNQTLR DLVTAKPQEY LGESIITKFG SSKELPFLFK 100
    VLSIEKVLSI QAHPDKKLGA QLHAADPKNY PDDNHKPEMA IAVTDFEGFC 150
    GFKPLDQLAK TLATVPELNE IIGQELVDEF ISGIKLPAEV GSQDDVNNRK 200
    LLQKVFGKLM NTDDDVIKQQ TAKLLERTDR EPQVFKDIDS RLPELIQRLN 250
    KQFPNDIGLF CGCLLLNHVG LNKGEAMFLQ AKDPHAYISG DIIECMAASD 300
    NVVRAGFTPK FKDVKNLVEM LTYSYESVEK QKMPLQEFPR SKGDAVKSVL 350
    YDPPIAEFSV LQTIFDKSKG GKQVIEGLNG PSIVIATNGK GTIQITGDDS 400
    TKQKIDTGYV FFVAPGSSIE LTADSANQDQ DFTTYRAFVE A 441
    Length:441
    Mass (Da):48,867
    Last modified:January 23, 2007 - v2
    Checksum:iAEDDE2AE9EDAD5EC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X82024 Genomic DNA. Translation: CAA57548.1.
    AACQ01000101 Genomic DNA. Translation: EAK95509.1.
    PIRiS55354.
    RefSeqiXP_714562.1. XM_709469.1.

    Genome annotation databases

    GeneIDi3643795.
    KEGGical:CaO19.1390.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X82024 Genomic DNA. Translation: CAA57548.1 .
    AACQ01000101 Genomic DNA. Translation: EAK95509.1 .
    PIRi S55354.
    RefSeqi XP_714562.1. XM_709469.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1PMI X-ray 1.70 A 2-441 [» ]
    ProteinModelPortali P34948.
    SMRi P34948. Positions 2-441.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5476.CAL0003967.

    Chemistry

    BindingDBi P34948.
    ChEMBLi CHEMBL3946.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3643795.
    KEGGi cal:CaO19.1390.

    Organism-specific databases

    CGDi CAL0003967. PMI1.

    Phylogenomic databases

    eggNOGi COG1482.
    KOi K01809.
    OrthoDBi EOG7WDNCR.

    Enzyme and pathway databases

    UniPathwayi UPA00126 ; UER00423 .

    Miscellaneous databases

    EvolutionaryTracei P34948.

    Family and domain databases

    Gene3Di 2.60.120.10. 3 hits.
    InterProi IPR001250. Man6P_Isoase-1.
    IPR016305. Mannose-6-P_Isomerase.
    IPR018050. Pmannose_isomerase-type1_CS.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view ]
    PANTHERi PTHR10309. PTHR10309. 1 hit.
    Pfami PF01238. PMI_typeI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
    PRINTSi PR00714. MAN6PISMRASE.
    SUPFAMi SSF51182. SSF51182. 1 hit.
    TIGRFAMsi TIGR00218. manA. 1 hit.
    PROSITEi PS00965. PMI_I_1. 1 hit.
    PS00966. PMI_I_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and heterologous expression of the Candida albicans gene PMI 1 encoding phosphomannose isomerase."
      Smith D.J., Proudfoot A.E.I., de Tiani M., Wells T.N.C., Payton M.A.
      Yeast 11:301-310(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 1006.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.
    3. "Identification of Cys-150 in the active site of phosphomannose isomerase from Candida albicans."
      Coulin F., Magnenat E., Proudfoot A.E.I., Payton M.A., Scully P., Wells T.N.C.
      Biochemistry 32:14139-14144(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHEMICAL LABELLING OF CYS-150, PARTIAL PROTEIN SEQUENCE.
    4. "Arginine 304 is an active site residue in phosphomannose isomerase from Candida albicans."
      Wells T.N.C., Scully P., Magnenat E.
      Biochemistry 33:5777-5782(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACTIVE SITE ARG-304, PROTEIN SEQUENCE OF 300-312.
    5. "The X-ray crystal structure of phosphomannose isomerase from Candida albicans at 1.7-A resolution."
      Cleasby A., Wonacott A., Skarzynski T., Hubbard R.E., Davies G.J., Proudfoot A.E.I., Bernard A.R., Payton M.A., Wells T.N.C.
      Nat. Struct. Biol. 3:470-479(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

    Entry informationi

    Entry nameiMPI_CANAL
    AccessioniPrimary (citable) accession number: P34948
    Secondary accession number(s): Q59YD6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Candida albicans
      Candida albicans: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3