Reviewed,
UniProtKB/Swiss-Prot P34947 (GRK5_HUMAN)
Last modified
January 19, 2010.
Version 100.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: G protein-coupled receptor kinase 5 EC=2.7.11.16 Alternative name(s): G protein-coupled receptor kinase GRK5 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 590 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Specifically phosphorylates the activated forms of G protein-coupled receptors. |
| Catalytic activity | ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate. |
| Tissue specificity | Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney. |
| Sequence similarities | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 590 | 590 | G protein-coupled receptor kinase 5 | PRO_0000085971 | |||||
Regions | |||||||||
| Domain | 53 – 171 | 119 | RGS | ||||||
| Domain | 186 – 448 | 263 | Protein kinase | ||||||
| Domain | 449 – 514 | 66 | AGC-kinase C-terminal | ||||||
| Nucleotide binding | 192 – 200 | 9 | ATP By similarity | ||||||
| Region | 1 – 185 | 185 | N-terminal | ||||||
Sites | |||||||||
| Active site | 311 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 215 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 484 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
| Modified residue | 485 | 1 | Phosphothreonine; by autocatalysis By similarity | ||||||
Natural variations | |||||||||
| Natural variant | 41 | 1 | Q → L: dbSNP rs17098707. Ref.7 | VAR_040517 | |||||
| Natural variant | 119 | 1 | A → V: dbSNP rs55980792. Ref.7 | VAR_040518 | |||||
| Natural variant | 122 | 1 | G → S: dbSNP rs55902633. Ref.7 | VAR_040519 | |||||
| Natural variant | 129 | 1 | T → M: dbSNP rs34679178. Ref.7 | VAR_040520 | |||||
| Natural variant | 141 | 1 | L → I: dbSNP rs56254855. Ref.7 | VAR_040521 | |||||
| Natural variant | 163 | 1 | D → E in a lung neuroendocrine carcinoma sample; somatic mutation. Ref.7 | VAR_040522 | |||||
| Natural variant | 172 | 1 | Q → H: dbSNP rs45630572. | VAR_059766 | |||||
| Natural variant | 304 | 1 | R → H: dbSNP rs2230349. Ref.7 | VAR_040523 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and expression of GRK5: a member of the G protein-coupled receptor kinase family." Kunapuli P., Benovic J.L. Proc. Natl. Acad. Sci. U.S.A. 90:5588-5592(1993) [PubMed: 7685906] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. Rogers J.Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate. |
| [5] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-484 AND THR-485, MASS SPECTROMETRY. |
| [6] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-484 AND THR-485, MASS SPECTROMETRY. Tissue: T-cell. |
| [7] | "Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. Stratton M.R.Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] LEU-41; VAL-119; SER-122; MET-129; ILE-141; GLU-163 AND HIS-304. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | L15388 mRNA. Translation: AAA58620.1. AL355273, AL355861, AL583824 Genomic DNA. Translation: CAI16487.1. AL355861, AL355273, AL583824 Genomic DNA. Translation: CAI15804.1. AL583824, AL355273, AL355861 Genomic DNA. Translation: CAI16759.1. CH471066 Genomic DNA. Translation: EAW49394.1. BC064506 mRNA. Translation: AAH64506.1. |
| IPI | IPI00414058. |
| PIR | A48277. B48682. |
| RefSeq | NP_005299.1. |
| UniGene | Hs.524625 |
3D structure databases | |
| SMR | P34947. Positions 23-532. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P34947. |
PTM databases | |
| PhosphoSite | P34947. |
Proteomic databases | |
| PRIDE | P34947. |
Genome annotation databases | |
| Ensembl | ENST00000392870; ENSP00000376609; ENSG00000198873; Homo sapiens. [Genome view] |
| GeneID | 2869. |
| KEGG | hsa:2869. |
| UCSC | uc001led.1. human. |
Organism-specific databases | |
| CTD | 2869. |
| GeneCards | GC10P120957. |
| H-InvDB | HIX0035405. |
| HGNC | HGNC:4544. GRK5. |
| HPA | CAB025579. |
| MIM | 600870. gene. |
| PharmGKB | PA180. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | HBG716704. |
| HOVERGEN | P34947. |
| InParanoid | P34947. |
| OMA | LECYIQF. |
| OrthoDB | EOG9Z0DR5. |
| PhylomeDB | P34947. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.16. 247. |
| Pathway_Interaction_DB | alphasynuclein_pathway. Alpha-synuclein signaling. |
| Reactome | REACT_14797. Signaling by GPCR. |
Gene expression databases | |
| ArrayExpress | P34947. |
| Bgee | P34947. |
| CleanEx | HS_GRK5. |
| Genevestigator | P34947. |
| GermOnline | ENSG00000198873. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000961. AGC-kinase_C. IPR000239. GPCR_kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR016137. Regulat_G_prot_signal_superfam. IPR017442. Se/Thr_prot_kinase-like_dom. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view] |
| PRINTS | PR00717. GPCRKINASE. |
| SMART | SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. PS50132. RGS. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 11319. |
| SOURCE | Search... |
Entry information
| Entry name | GRK5_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P34947 Secondary accession number(s): Q5T059 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 10 Human chromosome 10: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


