Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial

Gene

NDUFA9

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Cofactori

FADNote: Binds 1 FAD per subunit.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial
Alternative name(s):
Complex I-39kD
Short name:
CI-39kD
NADH-ubiquinone oxidoreductase 39 kDa subunit
Gene namesi
Name:NDUFA9
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 5

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrial respiratory chain complex I Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3535MitochondrionAdd
BLAST
Chaini36 – 380345NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrialPRO_0000019991Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei175 – 1751N6-succinyllysineBy similarity
Modified residuei189 – 1891N6-acetyllysineBy similarity

Post-translational modificationi

Acetylated on lysine residues. BLOC1S1 is required for acetylation (By similarity).By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP34943.
PeptideAtlasiP34943.
PRIDEiP34943.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits. This a component of the hydrophobic protein fraction (By similarity). Interacts with BLOC1S1 (By similarity). Interacts with SLC2A4 (By similarity).By similarity2 Publications

Protein-protein interaction databases

IntActiP34943. 3 interactions.
STRINGi9913.ENSBTAP00000007189.

Structurei

3D structure databases

ProteinModelPortaliP34943.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I NDUFA9 subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2865. Eukaryota.
COG0702. LUCA.
GeneTreeiENSGT00390000006865.
HOGENOMiHOG000168008.
HOVERGENiHBG006546.
InParanoidiP34943.
KOiK03953.
OMAiKSGTQMI.
OrthoDBiEOG7TF795.
TreeFamiTF105961.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF13460. NAD_binding_10. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34943-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAVHPRVV RVLPMSRSSV PALAASVFHS PPQRQLHHAV IPHGKGGRSS
60 70 80 90 100
VSGIVATVFG ATGFLGRYVV NHLGRMGSQV IVPHRCEPYD TMHLRPMGDL
110 120 130 140 150
GQIIFMDWNG RDKDSIRRAV EHSSVVINLV GREWETQNFD FEDVFVKIPQ
160 170 180 190 200
AIAQVSKEAG VEKFIHISHL NADIKSSSKY LRSKAVGEKE VRETFPEATI
210 220 230 240 250
IKPAEIFGRE DRFLNYFANI RWFGGVPLIS LGKKTVKQPV YIVDVTKGII
260 270 280 290 300
NAIKDPDARG KTFAFVGPSR YLLFDLVQYV FAVAHRPFLP YPLPHFAYRW
310 320 330 340 350
IGRLFEISPF EPWTTRDKVE RIHTTDKILP HLPGLEDLGV EATPLELKAI
360 370 380
EVLRRHRTYR WLSSEIEDVQ PAKTIPTSGP
Length:380
Mass (Da):42,849
Last modified:February 1, 1994 - v1
Checksum:iB453FA19D48D03CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59418 mRNA. Translation: CAA42053.1.
BC102359 mRNA. Translation: AAI02360.1.
PIRiS17676.
RefSeqiNP_991386.1. NM_205817.1.
UniGeneiBt.5541.

Genome annotation databases

EnsembliENSBTAT00000007189; ENSBTAP00000007189; ENSBTAG00000005465.
GeneIDi404188.
KEGGibta:404188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59418 mRNA. Translation: CAA42053.1.
BC102359 mRNA. Translation: AAI02360.1.
PIRiS17676.
RefSeqiNP_991386.1. NM_205817.1.
UniGeneiBt.5541.

3D structure databases

ProteinModelPortaliP34943.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP34943. 3 interactions.
STRINGi9913.ENSBTAP00000007189.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP34943.
PeptideAtlasiP34943.
PRIDEiP34943.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000007189; ENSBTAP00000007189; ENSBTAG00000005465.
GeneIDi404188.
KEGGibta:404188.

Organism-specific databases

CTDi4704.

Phylogenomic databases

eggNOGiKOG2865. Eukaryota.
COG0702. LUCA.
GeneTreeiENSGT00390000006865.
HOGENOMiHOG000168008.
HOVERGENiHBG006546.
InParanoidiP34943.
KOiK03953.
OMAiKSGTQMI.
OrthoDBiEOG7TF795.
TreeFamiTF105961.

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF13460. NAD_binding_10. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "NADH:ubiquinone oxidoreductase from bovine heart mitochondria. cDNA sequences of the import precursors of the nuclear-encoded 39 kDa and 42 kDa subunits."
    Fearnley I.M., Finel M., Skehel J.M., Walker J.E.
    Biochem. J. 278:821-829(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Heart.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.
  3. "Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme."
    Sazanov L.A., Peak-Chew S.Y., Fearnley I.M., Walker J.E.
    Biochemistry 39:7229-7235(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, SUBUNIT, IDENTIFICATION IN COMPLEX I.
  4. "Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry."
    Lemma-Gray P., Valusova E., Carroll C.A., Weintraub S.T., Musatov A., Robinson N.C.
    Anal. Biochem. 382:116-121(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION IN COMPLEX I.

Entry informationi

Entry nameiNDUA9_BOVIN
AccessioniPrimary (citable) accession number: P34943
Secondary accession number(s): Q3T0K3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 6, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.