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Reviewed, UniProtKB/Swiss-Prot P34932 (HSP74_HUMAN)

Last modified November 3, 2009. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Heat shock 70 kDa protein 4
Alternative name(s):
    Heat shock 70-related protein APG-2
    HSP70RY
Gene names
Name: HSPA4
Synonyms: APG2
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length840 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Subunit structure

Interacts with TJP1/ZO-1 By similarity.

Subcellular location

Cytoplasm Probable.

Post-translational modification

Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.6 Ref.7 Ref.8

Sequence similarities

Belongs to the heat shock protein 70 family.

Sequence caution

The sequence AAA02807.1 differs from that shown. Reason: Frameshift at positions 567, 586, 697, 715 and 722.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 840840Heat shock 70 kDa protein 4
PRO_0000078262

Amino acid modifications

Modified residue761Phosphoserine Ref.8
Modified residue891Phosphotyrosine Ref.6
Modified residue3361Phosphotyrosine Ref.6
Modified residue4301N6-acetyllysine Ref.10
Modified residue5381Phosphothreonine Ref.8
Modified residue5521Phosphoserine Ref.7
Modified residue6601Phosphotyrosine By similarity
Modified residue6791N6-acetyllysine Ref.10

Experimental info

Sequence conflict941L → W in AAA02807. Ref.4
Sequence conflict1901A → R in AAA02807. Ref.4
Sequence conflict583 – 5864NQLL → ESAI in AAA02807. Ref.4
Sequence conflict6221E → R in BAA75062. Ref.1
Sequence conflict6441D → G in BAA75062. Ref.1
Sequence conflict746 – 7494NNKL → EVTP in AAA02807. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P34932-1 [UniParc].

Last modified September 23, 2008. Version 4.
Checksum: 8B690A52A0729C2A

FASTA84094,331
        10         20         30         40         50         60 
MSVVGIDLGF QSCYVAVARA GGIETIANEY SDRCTPACIS FGPKNRSIGA AAKSQVISNA 

        70         80         90        100        110        120 
KNTVQGFKRF HGRAFSDPFV EAEKSNLAYD IVQLPTGLTG IKVTYMEEER NFTTEQVTAM 

       130        140        150        160        170        180 
LLSKLKETAE SVLKKPVVDC VVSVPCFYTD AERRSVMDAT QIAGLNCLRL MNETTAVALA 

       190        200        210        220        230        240 
YGIYKQDLPA LEEKPRNVVF VDMGHSAYQV SVCAFNRGKL KVLATAFDTT LGGRKFDEVL 

       250        260        270        280        290        300 
VNHFCEEFGK KYKLDIKSKI RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDVDVSGT 

       310        320        330        340        350        360 
MNRGKFLEMC NDLLARVEPP LRSVLEQTKL KKEDIYAVEI VGGATRIPAV KEKISKFFGK 

       370        380        390        400        410        420 
ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY PISLRWNSPA EEGSSDCEVF 

       430        440        450        460        470        480 
SKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ DLPYPDPAIA QFSVQKVTPQ SDGSSSKVKV 

       490        500        510        520        530        540 
KVRVNVHGIF SVSSASLVEV HKSEENEEPM ETDQNAKEEE KMQVDQEEPH VEEQQQQTPA 

       550        560        570        580        590        600 
ENKAESEEME TSQAGSKDKK MDQPPQAKKA KVKTSTVDLP IENQLLWQID REMLNLYIEN 

       610        620        630        640        650        660 
EGKMIMQDKL EKERNDAKNA VEEYVYEMRD KLSGEYEKFV SEDDRNSFTL KLEDTENWLY 

       670        680        690        700        710        720 
EDGEDQPKQV YVDKLAELKN LGQPIKIRFQ ESEERPKLFE ELGKQIQQYM KIISSFKNKE 

       730        740        750        760        770        780 
DQYDHLDAAD MTKVEKSTNE AMEWMNNKLN LQNKQSLTMD PVVKSKEIEA KIKELTSTCS 

       790        800        810        820        830        840 
PIISKPKPKV EPPKEEQKNA EQNGPVDGQG DNPGPQAAEQ GTDTAVPSDS DKKLPEMDID 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of human apg-1 and apg-2, members of the hsp110 family, cDNAs and chromosomal assignment of the genes."
Nonoguchi K., Fujita J.
Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed: 15372022] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain and Skin.
[4]"Molecular cloning of a novel human hsp70 from a B cell line and its assignment to chromosome 5."
Fathallah D.M., Cherif D., Dellagi K., Arnaout M.A.
J. Immunol. 151:810-813(1993) [PubMed: 8335910] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-749.
Tissue: Lymphocyte.
[5]Lubec G., Afjehi-Sadat L., Chen W.-Q., Sun Y.
Submitted (DEC-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 20-33; 111-124; 170-185; 222-234; 333-346; 361-374 AND 391-422, MASS SPECTROMETRY.
Tissue: Brain, Cajal-Retzius cell and Fetal brain cortex.
[6]"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-89 AND TYR-336, MASS SPECTROMETRY.
[7]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-552, MASS SPECTROMETRY.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76 AND THR-538, MASS SPECTROMETRY.
[9]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[10]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed: 19608861] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-430 AND LYS-679, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

AB023420 mRNA. Translation: BAA75062.1.
AC113410 Genomic DNA. No translation available.
BC110861 mRNA. Translation: AAI10862.1.
BC126122 mRNA. Translation: AAI26123.1.
BC126124 mRNA. Translation: AAI26125.1.
L12723 mRNA. Translation: AAA02807.1. Frameshift.
IPIIPI00002966.
PIRI56208.
RefSeqNP_002145.3.
UniGeneHs.90093

3D structure databases

HSSPHSSP built from PDB template 1BA1 based on UniProtKB P19120.
ModBaseSearch...

Protein-protein interaction databases

IntActP34932. 7 interactions.
STRINGP34932.

PTM databases

PhosphoSiteP34932.

2-D gel databases

DOSAC-COBS-2DPAGEP34932.
REPRODUCTION-2DPAGEIPI00002966.
P34932.

Proteomic databases

PRIDEP34932.

Genome annotation databases

EnsemblENST00000304858; ENSP00000302961; ENSG00000170606; Homo sapiens. [Genome view]
ENST00000321956; ENSP00000320397; ENSG00000170606; Homo sapiens. [Genome view]
GeneID3308.
KEGGhsa:3308.
UCSCuc003kyj.1. human.

Organism-specific databases

CTD3308.
GeneCardsGC05P132415.
H-InvDBHIX0005166.
HGNCHGNC:5237. HSPA4.
HPAHPA010023.
MIM601113. gene.
PharmGKBPA29503.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP34932.
HOVERGENP34932.
OMAQGDNPGP.

Gene expression databases

ArrayExpressP34932.
BgeeP34932.
CleanExHS_HSPA4.
GenevestigatorP34932.
GermOnlineENSG00000170606. Homo sapiens.

Family and domain databases

InterProIPR018181. Heat_shock_70_CS.
IPR001023. Hsp70.
IPR013126. Hsp_70.
[Graphical view]
PANTHERPTHR19375. Hsp70. 1 hit.
PfamPF00012. HSP70. 2 hits.
[Graphical view]
PRINTSPR00301. HEATSHOCK70.
ProDomPD000089. Hsp70. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00297. HSP70_1. False negative.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio13119.
SOURCESearch...

Entry information

Entry nameHSP74_HUMAN
AccessionPrimary (citable) accession number: P34932
Secondary accession number(s): O95756, Q2TAL4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: September 23, 2008
Last modified: November 3, 2009
This is version 97 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents