Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Heat shock 70 kDa protein 4

Gene

HSPA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • ATP binding Source: UniProtKB

GO - Biological processi

  • chaperone-mediated protein complex assembly Source: BHF-UCL
  • protein import into mitochondrial outer membrane Source: BHF-UCL
  • response to unfolded protein Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000170606-MONOMER.
ReactomeiR-HSA-3371453. Regulation of HSF1-mediated heat shock response.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock 70 kDa protein 4
Alternative name(s):
HSP70RY
Heat shock 70-related protein APG-2
Gene namesi
Name:HSPA4
Synonyms:APG2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:5237. HSPA4.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi3308.
OpenTargetsiENSG00000170606.
PharmGKBiPA29503.

Polymorphism and mutation databases

BioMutaiHSPA4.
DMDMi206729934.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000782621 – 840Heat shock 70 kDa protein 4Add BLAST840

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53N6-acetyllysineBy similarity1
Modified residuei76PhosphoserineCombined sources1
Modified residuei89PhosphotyrosineCombined sources1
Modified residuei336PhosphotyrosineCombined sources1
Modified residuei393PhosphoserineCombined sources1
Modified residuei415PhosphoserineBy similarity1
Modified residuei430N6-acetyllysineCombined sources1
Modified residuei538PhosphothreonineCombined sources1
Modified residuei546PhosphoserineCombined sources1
Modified residuei647PhosphoserineCombined sources1
Modified residuei660PhosphotyrosineBy similarity1
Modified residuei679N6-acetyllysineCombined sources1
Modified residuei756PhosphoserineCombined sources1
Modified residuei773N6-methyllysineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiP34932.
MaxQBiP34932.
PaxDbiP34932.
PeptideAtlasiP34932.
PRIDEiP34932.

2D gel databases

DOSAC-COBS-2DPAGEP34932.
REPRODUCTION-2DPAGEIPI00002966.
P34932.

PTM databases

iPTMnetiP34932.
PhosphoSitePlusiP34932.
SwissPalmiP34932.

Expressioni

Gene expression databases

BgeeiENSG00000170606.
CleanExiHS_HSPA4.
ExpressionAtlasiP34932. baseline and differential.
GenevisibleiP34932. HS.

Organism-specific databases

HPAiCAB025529.
HPA010023.

Interactioni

Subunit structurei

Interacts with TJP1/ZO-1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
AGTRAPQ6RW133EBI-356933,EBI-741181
APPBP2Q926245EBI-356933,EBI-743771

Protein-protein interaction databases

BioGridi109540. 339 interactors.
DIPiDIP-460N.
IntActiP34932. 42 interactors.
MINTiMINT-1159519.
STRINGi9606.ENSP00000302961.

Structurei

3D structure databases

ProteinModelPortaliP34932.
SMRiP34932.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiKOG0103. Eukaryota.
COG0443. LUCA.
GeneTreeiENSGT00390000016919.
HOGENOMiHOG000228138.
HOVERGENiHBG047955.
InParanoidiP34932.
KOiK09489.
OMAiDDRNTFT.
OrthoDBiEOG091G04Y6.
PhylomeDBiP34932.
TreeFamiTF105043.

Family and domain databases

Gene3Di1.20.1270.10. 2 hits.
2.60.34.10. 2 hits.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 2 hits.
SSF100934. SSF100934. 2 hits.
PROSITEiPS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P34932-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVVGIDLGF QSCYVAVARA GGIETIANEY SDRCTPACIS FGPKNRSIGA
60 70 80 90 100
AAKSQVISNA KNTVQGFKRF HGRAFSDPFV EAEKSNLAYD IVQLPTGLTG
110 120 130 140 150
IKVTYMEEER NFTTEQVTAM LLSKLKETAE SVLKKPVVDC VVSVPCFYTD
160 170 180 190 200
AERRSVMDAT QIAGLNCLRL MNETTAVALA YGIYKQDLPA LEEKPRNVVF
210 220 230 240 250
VDMGHSAYQV SVCAFNRGKL KVLATAFDTT LGGRKFDEVL VNHFCEEFGK
260 270 280 290 300
KYKLDIKSKI RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDVDVSGT
310 320 330 340 350
MNRGKFLEMC NDLLARVEPP LRSVLEQTKL KKEDIYAVEI VGGATRIPAV
360 370 380 390 400
KEKISKFFGK ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY
410 420 430 440 450
PISLRWNSPA EEGSSDCEVF SKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ
460 470 480 490 500
DLPYPDPAIA QFSVQKVTPQ SDGSSSKVKV KVRVNVHGIF SVSSASLVEV
510 520 530 540 550
HKSEENEEPM ETDQNAKEEE KMQVDQEEPH VEEQQQQTPA ENKAESEEME
560 570 580 590 600
TSQAGSKDKK MDQPPQAKKA KVKTSTVDLP IENQLLWQID REMLNLYIEN
610 620 630 640 650
EGKMIMQDKL EKERNDAKNA VEEYVYEMRD KLSGEYEKFV SEDDRNSFTL
660 670 680 690 700
KLEDTENWLY EDGEDQPKQV YVDKLAELKN LGQPIKIRFQ ESEERPKLFE
710 720 730 740 750
ELGKQIQQYM KIISSFKNKE DQYDHLDAAD MTKVEKSTNE AMEWMNNKLN
760 770 780 790 800
LQNKQSLTMD PVVKSKEIEA KIKELTSTCS PIISKPKPKV EPPKEEQKNA
810 820 830 840
EQNGPVDGQG DNPGPQAAEQ GTDTAVPSDS DKKLPEMDID
Length:840
Mass (Da):94,331
Last modified:September 23, 2008 - v4
Checksum:i8B690A52A0729C2A
GO
Isoform 2 (identifier: P34932-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     109-800: Missing.

Show »
Length:148
Mass (Da):15,689
Checksum:iC269BCA9925F562F
GO

Sequence cautioni

The sequence AAA02807 differs from that shown. Reason: Frameshift at positions 567, 586, 697, 715 and 722.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94L → W in AAA02807 (PubMed:8335910).Curated1
Sequence conflicti190A → R in AAA02807 (PubMed:8335910).Curated1
Sequence conflicti583 – 586NQLL → ESAI in AAA02807 (PubMed:8335910).Curated4
Sequence conflicti622E → R in BAA75062 (Ref. 1) Curated1
Sequence conflicti644D → G in BAA75062 (Ref. 1) Curated1
Sequence conflicti746 – 749NNKL → EVTP in AAA02807 (PubMed:8335910).Curated4

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056885109 – 800Missing in isoform 2. 2 PublicationsAdd BLAST692

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023420 mRNA. Translation: BAA75062.1.
BT007375 mRNA. Translation: AAP36039.1.
AC113410 Genomic DNA. No translation available.
BC002526 mRNA. Translation: AAH02526.1.
BC110861 mRNA. Translation: AAI10862.1.
BC126122 mRNA. Translation: AAI26123.1.
BC126124 mRNA. Translation: AAI26125.1.
L12723 mRNA. Translation: AAA02807.1. Frameshift.
CCDSiCCDS4166.1. [P34932-1]
PIRiI56208.
RefSeqiNP_002145.3. NM_002154.3. [P34932-1]
UniGeneiHs.90093.

Genome annotation databases

EnsembliENST00000304858; ENSP00000302961; ENSG00000170606. [P34932-1]
ENST00000617819; ENSP00000480688; ENSG00000170606. [P34932-2]
GeneIDi3308.
KEGGihsa:3308.
UCSCiuc003kyj.4. human. [P34932-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023420 mRNA. Translation: BAA75062.1.
BT007375 mRNA. Translation: AAP36039.1.
AC113410 Genomic DNA. No translation available.
BC002526 mRNA. Translation: AAH02526.1.
BC110861 mRNA. Translation: AAI10862.1.
BC126122 mRNA. Translation: AAI26123.1.
BC126124 mRNA. Translation: AAI26125.1.
L12723 mRNA. Translation: AAA02807.1. Frameshift.
CCDSiCCDS4166.1. [P34932-1]
PIRiI56208.
RefSeqiNP_002145.3. NM_002154.3. [P34932-1]
UniGeneiHs.90093.

3D structure databases

ProteinModelPortaliP34932.
SMRiP34932.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109540. 339 interactors.
DIPiDIP-460N.
IntActiP34932. 42 interactors.
MINTiMINT-1159519.
STRINGi9606.ENSP00000302961.

PTM databases

iPTMnetiP34932.
PhosphoSitePlusiP34932.
SwissPalmiP34932.

Polymorphism and mutation databases

BioMutaiHSPA4.
DMDMi206729934.

2D gel databases

DOSAC-COBS-2DPAGEP34932.
REPRODUCTION-2DPAGEIPI00002966.
P34932.

Proteomic databases

EPDiP34932.
MaxQBiP34932.
PaxDbiP34932.
PeptideAtlasiP34932.
PRIDEiP34932.

Protocols and materials databases

DNASUi3308.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304858; ENSP00000302961; ENSG00000170606. [P34932-1]
ENST00000617819; ENSP00000480688; ENSG00000170606. [P34932-2]
GeneIDi3308.
KEGGihsa:3308.
UCSCiuc003kyj.4. human. [P34932-1]

Organism-specific databases

CTDi3308.
DisGeNETi3308.
GeneCardsiHSPA4.
HGNCiHGNC:5237. HSPA4.
HPAiCAB025529.
HPA010023.
MIMi601113. gene.
neXtProtiNX_P34932.
OpenTargetsiENSG00000170606.
PharmGKBiPA29503.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0103. Eukaryota.
COG0443. LUCA.
GeneTreeiENSGT00390000016919.
HOGENOMiHOG000228138.
HOVERGENiHBG047955.
InParanoidiP34932.
KOiK09489.
OMAiDDRNTFT.
OrthoDBiEOG091G04Y6.
PhylomeDBiP34932.
TreeFamiTF105043.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000170606-MONOMER.
ReactomeiR-HSA-3371453. Regulation of HSF1-mediated heat shock response.

Miscellaneous databases

ChiTaRSiHSPA4. human.
GeneWikiiHSPA4.
GenomeRNAii3308.
PROiP34932.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170606.
CleanExiHS_HSPA4.
ExpressionAtlasiP34932. baseline and differential.
GenevisibleiP34932. HS.

Family and domain databases

Gene3Di1.20.1270.10. 2 hits.
2.60.34.10. 2 hits.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 2 hits.
SSF100934. SSF100934. 2 hits.
PROSITEiPS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHSP74_HUMAN
AccessioniPrimary (citable) accession number: P34932
Secondary accession number(s): O95756, Q2TAL4, Q9BUK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: September 23, 2008
Last modified: November 30, 2016
This is version 168 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.