P34927 (ASGR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asialoglycoprotein receptor 1
Short name=ASGP-R 1
Short name=ASGPR 1

Alternative name(s):
Hepatic lectin 1
Short name=HL-1
Short name=mHL-1

Gene names

Name:	Asgr1
Synonyms:	Asgr-1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
Subunit structure	Interacts with LASS2 By similarity.
Subcellular location	Membrane; Single-pass type II membrane protein.
Tissue specificity	Expressed exclusively in hepatic parenchymal cells.
Post-translational modification	Phosphorylated on a cytoplasmic Ser residue By similarity.
Miscellaneous	Calcium is required for ligand binding.
Sequence similarities	Contains 1 C-type lectin domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Membrane
Domain	Coiled coil Signal-anchor Transmembrane Transmembrane helix
Ligand	Calcium Lectin Metal-binding
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	cellular response to extracellular stimulus Inferred from direct assay. Source: MGI endocytosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein homodimerization activity Inferred from direct assay. Source: MGI sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 284	283	Asialoglycoprotein receptor 1		PRO_0000046651
Regions
	Topological domain	2 – 39	38	Cytoplasmic Potential
	Transmembrane	40 – 60	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	61 – 284	224	Extracellular Potential
	Domain	160 – 277	118	C-type lectin
	Coiled coil	59 – 117	59	Potential
	Motif	5 – 8	4	Endocytosis signal Potential
Sites
	Metal binding	190	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	196	1	Calcium 1 By similarity
	Metal binding	215	1	Calcium 2 By similarity
	Metal binding	239	1	Calcium 3 By similarity
	Metal binding	241	1	Calcium 3 By similarity
	Metal binding	252	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	252	1	Calcium 3 By similarity
	Metal binding	253	1	Calcium 2 By similarity
	Metal binding	264	1	Calcium 3 By similarity
	Metal binding	265	1	Calcium 3 By similarity
	Metal binding	277	1	Calcium 1 By similarity
	Binding site	239	1	Carbohydrate By similarity
	Binding site	243	1	Carbohydrate By similarity
	Binding site	252	1	Carbohydrate By similarity
Amino acid modifications
	Lipidation	35	1	S-palmitoyl cysteine By similarity
	Glycosylation	75	1	N-linked (GlcNAc...) Potential
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Glycosylation	146	1	N-linked (GlcNAc...) Potential
	Disulfide bond	153 ↔ 164		By similarity
	Disulfide bond	181 ↔ 276		By similarity
	Disulfide bond	254 ↔ 268		By similarity
Experimental info
	Sequence conflict	151	1	I → T in BAA02734. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P34927-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: FE1F0423410FA4A6	FASTA	284	32,604
	10 20 30 40 50 60 MTKDYQDFQH LDNDNDHHQL RRGPPPTPRL LQRLCSGSRL LLLSSSLSIL LLVVVCVITS 70 80 90 100 110 120 QNSQLREDLL ALRQNFSNLT VSTEDQVKAL STQGSSVGRK MKLVESKLEK QQKDLTEDHS 130 140 150 160 170 180 SLLLHVKQLV SDVRSLSCQM AAFRGNGSER ICCPINWVEY EGSCYWFSSS VRPWTEADKY 190 200 210 220 230 240 CQLENAHLVV VTSRDEQNFL QRHMGPLNTW IGLTDQNGPW KWVDGTDYET GFQNWRPEQP 250 260 270 280 DNWYGHGLGG GEDCAHFTTD GRWNDDVCRR PYRWVCETKL DKAN

References

[1]	"Determination of mouse major asialoglycoprotein receptor cDNA sequence." Takezawa R., Shinzawa K., Watanabe Y., Akaike T. Biochim. Biophys. Acta 1172:220-222(1993) [PubMed: 8439566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The major form of the murine asialoglycoprotein receptor: cDNA sequence and expression in liver, testis and epididymis." Monroe R.S., Huber B.E. Gene 148:237-244(1994) [PubMed: 7958950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Liver.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

Hepatic asialoglycoprotein receptor subunit 1

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13517 mRNA. Translation: BAA02734.1. U09362 mRNA. Translation: AAB60441.1. U08372 mRNA. Translation: AAB60440.1.
IPI	IPI00555036.
RefSeq	NP_033844.1.
UniGene	Mm.6559.
3D structure databases
ProteinModelPortal	P34927.
SMR	P34927. Positions 153-280.
ModBase	Search...
Protein-protein interaction databases
STRING	P34927.
Proteomic databases
PRIDE	P34927.
Genome annotation databases
Ensembl	ENSMUST00000018699; ENSMUSP00000018699; ENSMUSG00000020884; Mus musculus. [Genome view] ENSMUST00000092959; ENSMUSP00000090637; ENSMUSG00000020884; Mus musculus. [Genome view]
GeneID	11889.
KEGG	mmu:11889.
Organism-specific databases
CTD	11889.
MGI	MGI:88081. Asgr1.
Phylogenomic databases
eggNOG	roNOG11202.
HOGENOM	HBG505903.
HOVERGEN	HBG000270.
InParanoid	P34927.
Gene expression databases
ArrayExpress	P34927.
Bgee	P34927.
CleanEx	MM_ASGR1.
Genevestigator	P34927.
GermOnline	ENSMUSG00000020884. Mus musculus.
Family and domain databases
InterPro	IPR002353. AntifreezeII. IPR001304. C-type_lectin. IPR016186. C-type_lectin-like. IPR018378. C-type_lectin_CS. IPR016187. C-type_lectin_fold. IPR005640. Lectin_N. [Graphical view]
Gene3D	G3DSA:3.10.100.10. C-type_lectin-like. 1 hit.
Pfam	PF00059. Lectin_C. 1 hit. PF03954. Lectin_N. 1 hit. [Graphical view]
PRINTS	PR00356. ANTIFREEZEII.
SMART	SM00034. CLECT. 1 hit. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 1 hit.
PROSITE	PS00615. C_TYPE_LECTIN_1. 1 hit. PS50041. C_TYPE_LECTIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

ASGR1_MOUSE

Accession

Primary (citable) accession number: P34927
Secondary accession number(s): Q64363

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	January 23, 2007
Last modified:	August 10, 2010
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)