Reviewed, UniProtKB/Swiss-Prot P34919 (G3PA_CHOCR)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase, chloroplastic
    EC=1.2.1.13
Alternative name(s):
    NADP-dependent glyceraldehydephosphate dehydrogenase

Gene names

Name:

GAPA

Organism

Chondrus crispus (Carragheen)

Taxonomic identifier

2769 [NCBI]

Taxonomic lineage

Eukaryota › Rhodophyta › Florideophyceae › Gigartinales › Gigartinaceae › Chondrus

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Homotetramer By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 76	76	Chloroplast Potential
	Chain	77 – 414	338	Glyceraldehyde-3-phosphate dehydrogenase, chloroplastic		PRO_0000010419
Regions
	Nucleotide binding	88 – 89	2	NADP By similarity
	Region	230 – 232	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	289 – 290	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	231	1	Nucleophile By similarity
	Binding site	112	1	NADP By similarity
	Binding site	156	1	NADP; via carbonyl oxygen By similarity
	Binding site	261	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	276	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	312	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	394	1	NADP By similarity
	Site	258	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34919-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 065DE62CE1064111	FASTA	414	44,459
	10 20 30 40 50 60 MAFVAPVATV RATTKSSVCQ VQGRSTFAQF SGMKKVNQSS RLQPAQSGSA FGGYSDANDA 70 80 90 100 110 120 FYTRVSGIVA ATFGPTMKVR VAINGFGRIG RNFIRCWAGR SDSNMEVVCI NDTSGVKTAS 130 140 150 160 170 180 HLLKYDSILG TFDADVSAGE DTISVNGKTI KIVSNRNPLQ LPWKEMNIDI VVEATGVFVD 190 200 210 220 230 240 APGAGKHIEA GAKKVLITAP GKGDGIGTFV VGVNEKDYSH DKYDIVSNAS CTTNCMAPFM 250 260 270 280 290 300 KVLDDEFGVV RGMMTTTHSY TGDQRLLDAG HRDLRRARSA ALNIVPTTTG AAKAVALVVP 310 320 330 340 350 360 SLKGKLNGIA LRVPTPNVSV CDVVMQVNKK TFKEEVNGAL LKASEGAMKG IIKYSDEPLV 370 380 390 400 410 SCDHRGTDES TIIDSSLTMV MGDDMIKVVA WYDNEWGYSQ RVVDLGEVMA RQWK

References

[1]

"The evolutionary origin of red algae as deduced from the nuclear genes encoding cytosolic and chloroplast glyceraldehyde-3-phosphate dehydrogenases from Chondrus crispus." Liaud M.-F., Valentin C., Martin W., Bouget F.Y., Kloareg B., Cerff R. J. Mol. Evol. 38:319-327(1994) [PubMed: 8007000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Stackhouse.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X73035 mRNA. Translation: CAA51516.1.
3D structure databases
HSSP	HSSP built from PDB template 1NBO based on UniProtKB P19866.
SMR	P34919. Positions 78-414.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.2.1.13. 190989.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006424. Glyceraldehyde-3-P_DH_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PA_CHOCR

Accession

Primary (citable) accession number: P34919

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)