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Protein

General negative regulator of transcription subunit 4

Gene

MOT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri33 – 78RING-typePROSITE-ProRule annotationAdd BLAST46
Zinc fingeri229 – 256C3H1-typePROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

GO - Biological processi

  • deadenylation-independent decapping of nuclear-transcribed mRNA Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • proteasomal protein catabolic process Source: SGD
  • protein polyubiquitination Source: SGD
  • protein ubiquitination Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30242-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
General negative regulator of transcription subunit 4
Alternative name(s):
Modulator of transcription 2
Gene namesi
Name:MOT2
Synonyms:CCL1, NOT4, SIG1, SSF1
Ordered Locus Names:YER068W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER068W.
SGDiS000000870. MOT2.

Subcellular locationi

GO - Cellular componenti

  • CCR4-NOT core complex Source: SGD
  • cytoplasm Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000816811 – 587General negative regulator of transcription subunit 4Add BLAST587

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki270Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei310PhosphothreonineCombined sources1
Modified residuei312PhosphoserineCombined sources1
Modified residuei326PhosphothreonineCombined sources1
Modified residuei360PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP34909.
PRIDEiP34909.

PTM databases

iPTMnetiP34909.

Interactioni

Subunit structurei

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CAF40P538295EBI-12174,EBI-28306
CCR4P313844EBI-12174,EBI-4396
CDC39P256554EBI-12174,EBI-12139
POP2P390083EBI-12174,EBI-13629

Protein-protein interaction databases

BioGridi36811. 175 interactors.
DIPiDIP-2255N.
IntActiP34909. 23 interactors.
MINTiMINT-617490.

Structurei

Secondary structure

1587
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni34 – 36Combined sources3
Turni42 – 46Combined sources5
Helixi58 – 66Combined sources9
Beta strandi68 – 70Combined sources3
Turni75 – 77Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AIEX-ray2.80A30-83[»]
5AJDX-ray3.62B/D/F/H/J/L418-477[»]
ProteinModelPortaliP34909.
SMRiP34909.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini137 – 228RRMPROSITE-ProRule annotationAdd BLAST92

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili94 – 128Sequence analysisAdd BLAST35

Sequence similaritiesi

Contains 1 C3H1-type zinc finger.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri33 – 78RING-typePROSITE-ProRule annotationAdd BLAST46
Zinc fingeri229 – 256C3H1-typePROSITE-ProRule annotationAdd BLAST28

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000000068.
HOGENOMiHOG000248860.
InParanoidiP34909.
KOiK10643.
OMAiEEDYCPL.
OrthoDBiEOG092C0PJB.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
IPR000571. Znf_CCCH.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
SMARTiSM00361. RRM_1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34909-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNPHVQENL QAIHNALSNF DTSFLSEDEE DYCPLCIEPM DITDKNFFPC
60 70 80 90 100
PCGYQICQFC YNNIRQNPEL NGRCPACRRK YDDENVRYVT LSPEELKMER
110 120 130 140 150
AKLARKEKER KHREKERKEN EYTNRKHLSG TRVIQKNLVY VVGINPPVPY
160 170 180 190 200
EEVAPTLKSE KYFGQYGKIN KIVVNRKTPH SNNTTSEHYH HHSPGYGVYI
210 220 230 240 250
TFGSKDDAAR CIAQVDGTYM DGRLIKAAYG TTKYCSSYLR GLPCPNPNCM
260 270 280 290 300
FLHEPGEEAD SFNKRELHNK QQAQQQSGGT AFTRSGIHNN ISTSTAGSNT
310 320 330 340 350
NLLSENFTGT PSPAAMRAQL HHDSHTNAGT PVLTPAPVPA GSNPWGVTQS
360 370 380 390 400
ATPVTSINLS KNSSSINLPT LNDSLGHHTT PTTENTITST TTTTNTNATS
410 420 430 440 450
HSHGSKKKQS LAAEEYKDPY DALGNAVDFL DARLHSLSNY QKRPISIKSN
460 470 480 490 500
IIDEETYKKY PSLFSWDKIE ASKKSDNTLA NKLVEILAIK PIDYTASVVQ
510 520 530 540 550
FLQSVNVGVN DNITITDNTK TPTQPIRLQT VSQQIQPPLN VSTPPPGIFG
560 570 580
PQHKVPIQQQ QMGDTSSRNS SDLLNQLING RKIIAGN
Length:587
Mass (Da):65,354
Last modified:February 1, 1994 - v1
Checksum:i8847084BD9BF48B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96736 Genomic DNA. Translation: AAC37413.1.
L26309 Unassigned DNA. Translation: AAB00326.1.
U18813 Genomic DNA. Translation: AAB64604.1.
BK006939 Genomic DNA. Translation: DAA07727.1.
PIRiA56015.
RefSeqiNP_010991.3. NM_001178959.3.

Genome annotation databases

EnsemblFungiiYER068W; YER068W; YER068W.
GeneIDi856799.
KEGGisce:YER068W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96736 Genomic DNA. Translation: AAC37413.1.
L26309 Unassigned DNA. Translation: AAB00326.1.
U18813 Genomic DNA. Translation: AAB64604.1.
BK006939 Genomic DNA. Translation: DAA07727.1.
PIRiA56015.
RefSeqiNP_010991.3. NM_001178959.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AIEX-ray2.80A30-83[»]
5AJDX-ray3.62B/D/F/H/J/L418-477[»]
ProteinModelPortaliP34909.
SMRiP34909.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36811. 175 interactors.
DIPiDIP-2255N.
IntActiP34909. 23 interactors.
MINTiMINT-617490.

PTM databases

iPTMnetiP34909.

Proteomic databases

MaxQBiP34909.
PRIDEiP34909.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER068W; YER068W; YER068W.
GeneIDi856799.
KEGGisce:YER068W.

Organism-specific databases

EuPathDBiFungiDB:YER068W.
SGDiS000000870. MOT2.

Phylogenomic databases

GeneTreeiENSGT00390000000068.
HOGENOMiHOG000248860.
InParanoidiP34909.
KOiK10643.
OMAiEEDYCPL.
OrthoDBiEOG092C0PJB.

Enzyme and pathway databases

BioCyciYEAST:G3O-30242-MONOMER.

Miscellaneous databases

PROiP34909.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
IPR000571. Znf_CCCH.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
SMARTiSM00361. RRM_1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNOT4_YEAST
AccessioniPrimary (citable) accession number: P34909
Secondary accession number(s): D3DLX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 170 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.