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Protein

General negative regulator of transcription subunit 4

Gene

MOT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.1 Publication

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri33 – 78RING-typePROSITE-ProRule annotationAdd BLAST46
Zinc fingeri229 – 256C3H1-typePROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • RNA binding Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  • deadenylation-independent decapping of nuclear-transcribed mRNA Source: SGD
  • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: GO_Central
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • proteasomal protein catabolic process Source: SGD
  • protein polyubiquitination Source: SGD
  • protein ubiquitination Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Repressor, RNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30242-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
General negative regulator of transcription subunit 4
Alternative name(s):
Modulator of transcription 2
Gene namesi
Name:MOT2
Synonyms:CCL1, NOT4, SIG1, SSF1
Ordered Locus Names:YER068W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER068W
SGDiS000000870 MOT2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000816811 – 587General negative regulator of transcription subunit 4Add BLAST587

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki270Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei310PhosphothreonineCombined sources1
Modified residuei312PhosphoserineCombined sources1
Modified residuei326PhosphothreonineCombined sources1
Modified residuei360PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP34909
PaxDbiP34909
PRIDEiP34909

PTM databases

iPTMnetiP34909

Interactioni

Subunit structurei

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36811, 184 interactors
DIPiDIP-2255N
IntActiP34909, 25 interactors
MINTiP34909
STRINGi4932.YER068W

Structurei

Secondary structure

1587
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni34 – 36Combined sources3
Turni42 – 46Combined sources5
Helixi58 – 66Combined sources9
Beta strandi68 – 70Combined sources3
Turni75 – 77Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AIEX-ray2.80A30-83[»]
5AJDX-ray3.62B/D/F/H/J/L418-477[»]
ProteinModelPortaliP34909
SMRiP34909
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini137 – 228RRMPROSITE-ProRule annotationAdd BLAST92

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili94 – 128Sequence analysisAdd BLAST35

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri33 – 78RING-typePROSITE-ProRule annotationAdd BLAST46
Zinc fingeri229 – 256C3H1-typePROSITE-ProRule annotationAdd BLAST28

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000000068
HOGENOMiHOG000248860
InParanoidiP34909
KOiK10643
OMAiPMDITDK
OrthoDBiEOG092C0PJB

Family and domain databases

CDDicd12438 RRM_CNOT4, 1 hit
Gene3Di3.30.40.10, 1 hit
3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR034261 CNOT4_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR003954 RRM_dom_euk
IPR000571 Znf_CCCH
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
SMARTiView protein in SMART
SM00361 RRM_1, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
PS50103 ZF_C3H1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

P34909-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNPHVQENL QAIHNALSNF DTSFLSEDEE DYCPLCIEPM DITDKNFFPC
60 70 80 90 100
PCGYQICQFC YNNIRQNPEL NGRCPACRRK YDDENVRYVT LSPEELKMER
110 120 130 140 150
AKLARKEKER KHREKERKEN EYTNRKHLSG TRVIQKNLVY VVGINPPVPY
160 170 180 190 200
EEVAPTLKSE KYFGQYGKIN KIVVNRKTPH SNNTTSEHYH HHSPGYGVYI
210 220 230 240 250
TFGSKDDAAR CIAQVDGTYM DGRLIKAAYG TTKYCSSYLR GLPCPNPNCM
260 270 280 290 300
FLHEPGEEAD SFNKRELHNK QQAQQQSGGT AFTRSGIHNN ISTSTAGSNT
310 320 330 340 350
NLLSENFTGT PSPAAMRAQL HHDSHTNAGT PVLTPAPVPA GSNPWGVTQS
360 370 380 390 400
ATPVTSINLS KNSSSINLPT LNDSLGHHTT PTTENTITST TTTTNTNATS
410 420 430 440 450
HSHGSKKKQS LAAEEYKDPY DALGNAVDFL DARLHSLSNY QKRPISIKSN
460 470 480 490 500
IIDEETYKKY PSLFSWDKIE ASKKSDNTLA NKLVEILAIK PIDYTASVVQ
510 520 530 540 550
FLQSVNVGVN DNITITDNTK TPTQPIRLQT VSQQIQPPLN VSTPPPGIFG
560 570 580
PQHKVPIQQQ QMGDTSSRNS SDLLNQLING RKIIAGN
Length:587
Mass (Da):65,354
Last modified:February 1, 1994 - v1
Checksum:i8847084BD9BF48B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96736 Genomic DNA Translation: AAC37413.1
L26309 Unassigned DNA Translation: AAB00326.1
U18813 Genomic DNA Translation: AAB64604.1
BK006939 Genomic DNA Translation: DAA07727.1
PIRiA56015
RefSeqiNP_010991.3, NM_001178959.3

Genome annotation databases

EnsemblFungiiYER068W; YER068W; YER068W
GeneIDi856799
KEGGisce:YER068W

Similar proteinsi

Entry informationi

Entry nameiNOT4_YEAST
AccessioniPrimary (citable) accession number: P34909
Secondary accession number(s): D3DLX3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 182 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health