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Reviewed, UniProtKB/Swiss-Prot P34909 (NOT4_YEAST)

Last modified November 24, 2009. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    General negative regulator of transcription subunit 4
Gene names
Name: NOT4
Synonyms: CCL1, MOT2, SIG1, SSF1
Ordered Locus Names: YER068W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length587 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery. Ref.6

Subunit structure

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex. Ref.7

Subcellular location

Cytoplasm Probable. Nucleus.

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium. Ref.9

Sequence similarities

Contains 1 C3H1-type zinc finger.

Contains 1 RING-type zinc finger.

Contains 1 RRM (RNA recognition motif) domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 587587General negative regulator of transcription subunit 4
PRO_0000081681

Regions

Domain137 – 22892RRM
Zinc finger33 – 7846RING-type
Zinc finger229 – 25628C3H1-type
Coiled coil94 – 12835 Potential

Amino acid modifications

Modified residue921Phosphoserine Ref.12
Modified residue2981Phosphoserine Ref.11
Modified residue3001Phosphothreonine Ref.12
Modified residue3101Phosphothreonine Ref.10
Modified residue3121Phosphoserine Ref.12 Ref.10
Modified residue3521Phosphothreonine Ref.12
Modified residue5421Phosphoserine Ref.12
Modified residue5431Phosphothreonine Ref.12

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Sequences

Sequence LengthMass (Da)Tools
P34909-1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 8847084BD9BF48B7

FASTA58765,354
        10         20         30         40         50         60 
MMNPHVQENL QAIHNALSNF DTSFLSEDEE DYCPLCIEPM DITDKNFFPC PCGYQICQFC 

        70         80         90        100        110        120 
YNNIRQNPEL NGRCPACRRK YDDENVRYVT LSPEELKMER AKLARKEKER KHREKERKEN 

       130        140        150        160        170        180 
EYTNRKHLSG TRVIQKNLVY VVGINPPVPY EEVAPTLKSE KYFGQYGKIN KIVVNRKTPH 

       190        200        210        220        230        240 
SNNTTSEHYH HHSPGYGVYI TFGSKDDAAR CIAQVDGTYM DGRLIKAAYG TTKYCSSYLR 

       250        260        270        280        290        300 
GLPCPNPNCM FLHEPGEEAD SFNKRELHNK QQAQQQSGGT AFTRSGIHNN ISTSTAGSNT 

       310        320        330        340        350        360 
NLLSENFTGT PSPAAMRAQL HHDSHTNAGT PVLTPAPVPA GSNPWGVTQS ATPVTSINLS 

       370        380        390        400        410        420 
KNSSSINLPT LNDSLGHHTT PTTENTITST TTTTNTNATS HSHGSKKKQS LAAEEYKDPY 

       430        440        450        460        470        480 
DALGNAVDFL DARLHSLSNY QKRPISIKSN IIDEETYKKY PSLFSWDKIE ASKKSDNTLA 

       490        500        510        520        530        540 
NKLVEILAIK PIDYTASVVQ FLQSVNVGVN DNITITDNTK TPTQPIRLQT VSQQIQPPLN 

       550        560        570        580 
VSTPPPGIFG PQHKVPIQQQ QMGDTSSRNS SDLLNQLING RKIIAGN

« Hide

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References

« Hide 'large scale' references
[1]"Molecular characterization of SIG1, a Saccharomyces cerevisiae gene involved in negative regulation of G-protein-mediated signal transduction."
Leberer E., Dignard D., Harcus D., Whiteway M., Thomas D.Y.
EMBO J. 13:3050-3064(1994) [PubMed: 8039500] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"MOT2 encodes a negative regulator of gene expression that affects basal expression of pheromone-responsive genes in Saccharomyces cerevisiae."
Cade R.M., Errede B.
Mol. Cell. Biol. 14:3139-3149(1994) [PubMed: 8164669] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[3]"The yeast MOT2 gene encodes a putative zinc finger protein that serves as a global negative regulator affecting expression of several categories of genes, including mating-pheromone-responsive genes."
Irie K., Yamaguchi K., Kawase K., Matsumoto K.
Mol. Cell. Biol. 14:3150-3157(1994) [PubMed: 8164670] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed: 9169868] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[5]"NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization."
Collart M.A., Struhl K.
Genes Dev. 8:525-537(1994) [PubMed: 7926748] [Abstract]
Cited for: CHARACTERIZATION.
[6]"The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively."
Liu H.Y., Badarinarayana V., Audino D.C., Rappsilber J., Mann M., Denis C.L.
EMBO J. 17:1096-1106(1998) [PubMed: 9463387] [Abstract]
Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, FUNCTION OF THE CCR4-NOT CORE COMPLEX IN TRANSCRIPTIONAL REGULATION.
[7]"The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5."
Bai Y., Salvadore C., Chiang Y.C., Collart M.A., Liu H.Y., Denis C.L.
Mol. Cell. Biol. 19:6642-6651(1999) [PubMed: 10490603] [Abstract]
Cited for: INTERACTION WITH NOT1.
[8]"Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex."
Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L.
J. Mol. Biol. 314:683-694(2001) [PubMed: 11733989] [Abstract]
Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-310 AND SER-312, MASS SPECTROMETRY.
[11]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-298, MASS SPECTROMETRY.
[12]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92; THR-300; SER-312; THR-352; SER-542 AND THR-543, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M96736 Genomic DNA. Translation: AAC37413.1.
L26309 Unassigned DNA. Translation: AAB00326.1.
U18813 Genomic DNA. Translation: AAB64604.1.
PIRA56015.
RefSeqNP_010991.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:2255N.
IntActP34909. 26 interactions.
STRINGP34909.

Proteomic databases

PeptideAtlasP34909.

Genome annotation databases

EnsemblYER068W; YER068W; YER068W; Saccharomyces cerevisiae. [Genome view]
GeneID856799.
KEGGsce:YER068W.
NMPDRfig|4932.3.peg.2056.

Organism-specific databases

CYGDYER068w.
SGDS000000870. MOT2.

Phylogenomic databases

HOGENOMP34909.
OMAYGKINKI
OrthoDBEOG9M0GHW

Gene expression databases

ArrayExpressP34909.
GenevestigatorP34909.
GermOnlineYER068W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR012677. a_b_plait_nuc_bd.
IPR003954. RRM_1.
IPR000504. RRM_RNP1.
IPR000571. Znf_CCCH.
IPR001841. Znf_RING.
[Graphical view]
Gene3DG3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
PfamPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTSM00184. RING. 1 hit.
SM00361. RRM_1. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 1 hit.
PS00518. ZF_RING_1. False negative.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio983041.

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Entry information

Entry nameNOT4_YEAST
AccessionPrimary (citable) accession number: P34909
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 24, 2009
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents