P34908 (BRAF_COTJA) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 93.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Serine/threonine-protein kinase B-raf EC=2.7.11.1 Alternative name(s): Proto-oncogene B-Raf Proto-oncogene c-Rmil Serine/threonine-protein kinase Rmil | ||||
| Gene names |
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| Organism | Coturnix coturnix japonica (Japanese quail) (Coturnix japonica) | ||||
| Taxonomic identifier | 93934 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Perdicinae › Coturnix |
Protein attributes
| Sequence length | 807 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | May play a role in transducing specific signals in neural cells. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Cofactor | Binds 2 zinc ions per subunit Potential. |
| Subcellular location | Nucleus. Cytoplasm By similarity. Cell membrane By similarity. |
| Tissue specificity | Expressed preferentially in neural tissue. |
| Post-translational modification | Phosphorylated. |
| Sequence similarities | Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. Contains 1 RBD (Ras-binding) domain. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform Long (identifier: P34908-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Short (identifier: P34908-2) The sequence of this isoform differs from the canonical sequence as follows: 393-432: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 807 | 807 | Serine/threonine-protein kinase B-raf | PRO_0000085668 | |||||
Regions | |||||||||
| Domain | 155 – 227 | 73 | RBD | ||||||
| Domain | 497 – 757 | 261 | Protein kinase | ||||||
| Zinc finger | 234 – 280 | 47 | Phorbol-ester/DAG-type | ||||||
| Nucleotide binding | 503 – 511 | 9 | ATP By similarity | ||||||
| Compositional bias | 122 – 129 | 8 | Poly-Ser | ||||||
| Compositional bias | 248 – 280 | 33 | Cys-rich | ||||||
Sites | |||||||||
| Active site | 616 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 235 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 248 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 251 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 261 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 264 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 269 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 272 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 280 | 1 | Zinc 1 By similarity | ||||||
| Binding site | 523 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 790 | 1 | Phosphoserine; by MAPK1 By similarity | ||||||
| Modified residue | 793 | 1 | Phosphothreonine; by MAPK1 By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 393 – 432 | 40 | Missing in isoform Short. | VSP_004799 | |||||
Sequences
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References
| [1] | "Quail neuroretina c-Rmil(B-raf) proto-oncogene cDNAs encode two proteins of 93.5 and 95 kDa resulting from alternative splicing." Eychene A., Barnier J.V., Dezelee P., Marx M., Laugier D., Calogeraki I., Calothy G. Oncogene 7:1315-1323(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT). |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M80846 mRNA. Translation: AAA49493.1. M80845 mRNA. Translation: AAA49492.1. |
3D structure databases | |
| ProteinModelPortal | P34908. |
| SMR | P34908. Positions 157-227, 232-283. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Phylogenomic databases | |
| HOVERGEN | HBG001886. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.1. 1673. |
Family and domain databases | |
| InterPro | IPR020454. DAG/PE-bd. IPR011009. Kinase-like_dom. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR003116. Raf-like_ras-bd. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view] |
| Pfam | PF00130. C1_1. 1 hit. PF07714. Pkinase_Tyr. 1 hit. PF02196. RBD. 1 hit. [Graphical view] |
| PRINTS | PR00008. DAGPEDOMAIN. |
| SMART | SM00109. C1. 1 hit. SM00455. RBD. 1 hit. [Graphical view] |
| SUPFAM | SSF56112. Kinase_like. 1 hit. |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50898. RBD. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | BRAF_COTJA | ||||||||
| Accession | Primary (citable) accession number: P34908 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |