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Protein

Modification methylase FnuDI

Gene

fnuDIM

Organism
Fusobacterium nucleatum
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the FnuDI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3402. M.FnuDI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase FnuDI (EC:2.1.1.37)
Short name:
M.FnuDI
Alternative name(s):
Cytosine-specific methyltransferase FnuDI
Gene namesi
Name:fnuDIM
OrganismiFusobacterium nucleatum
Taxonomic identifieri851 [NCBI]
Taxonomic lineageiBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Modification methylase FnuDIPRO_0000087876Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP34906.
SMRiP34906. Positions 1-327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 330330SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34906-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLLSLFSGA GGLDLGFERA GFEIIVANEY DKTIWETYEK NHKAKLIKKD
60 70 80 90 100
IREILSEELP KSDGIIGGPP CQSWSEAGSL RGINDPRGKL FYEYIRILKD
110 120 130 140 150
IQPKFFLAEN VKGMLSKRNT EAVKDIIKEF EEAGYNVFIK LLNAFDYGVA
160 170 180 190 200
QDRERVFYVG FRKDLNISNF EFPYPISEKE RKYLKDSIWD LKDNALPGKD
210 220 230 240 250
KNKTNADDCI VENHEYLTGS YSTIFMSRNR VRQWEQPAFT VQASGRQCQL
260 270 280 290 300
HPQAPTMIKI DKNMYKFVAG KENLYRRLSI RECARIQGFP DTFKFYYTSL
310 320 330 340
EDGYKMVGNA VPVDLAYIIA KRIKETLTDK EKIKKEIRQK TLFD
Length:344
Mass (Da):39,913
Last modified:February 1, 1994 - v1
Checksum:i7B0CB6C9721FE91C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051374 Genomic DNA. Translation: AAC05695.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051374 Genomic DNA. Translation: AAC05695.1.

3D structure databases

ProteinModelPortaliP34906.
SMRiP34906. Positions 1-327.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3402. M.FnuDI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Method for producing the FnuDI restriction endonuclease and methylase."
    Zhang B.-H., Wilson G.G.
    Patent number US4988620, 29-JAN-1991
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: D.

Entry informationi

Entry nameiMTF1_FUSNU
AccessioniPrimary (citable) accession number: P34906
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: January 7, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.