Reviewed, UniProtKB/Swiss-Prot P34906 (MTF1_FUSNU)

Names and origin

Protein names

Recommended name:
    Modification methylase FnuDI
      Short name=M.FnuDI
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase FnuDI

Gene names

Name:

fnuDIM

Organism

Fusobacterium nucleatum

Taxonomic identifier

851 [NCBI]

Taxonomic lineage

Bacteria › Fusobacteria › Fusobacterales › Fusobacteriaceae › Fusobacterium

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the FnuDI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Modification methylase FnuDI		PRO_0000087876
Sites
	Active site	71	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34906-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 7B0CB6C9721FE91C	FASTA	344	39,913
	10 20 30 40 50 60 MKLLSLFSGA GGLDLGFERA GFEIIVANEY DKTIWETYEK NHKAKLIKKD IREILSEELP 70 80 90 100 110 120 KSDGIIGGPP CQSWSEAGSL RGINDPRGKL FYEYIRILKD IQPKFFLAEN VKGMLSKRNT 130 140 150 160 170 180 EAVKDIIKEF EEAGYNVFIK LLNAFDYGVA QDRERVFYVG FRKDLNISNF EFPYPISEKE 190 200 210 220 230 240 RKYLKDSIWD LKDNALPGKD KNKTNADDCI VENHEYLTGS YSTIFMSRNR VRQWEQPAFT 250 260 270 280 290 300 VQASGRQCQL HPQAPTMIKI DKNMYKFVAG KENLYRRLSI RECARIQGFP DTFKFYYTSL 310 320 330 340 EDGYKMVGNA VPVDLAYIIA KRIKETLTDK EKIKKEIRQK TLFD

References

[1]	"Method for producing the FnuDI restriction endonuclease and methylase." Zhang B.-H., Wilson G.G. Patent number US4988620, 29-JAN-1991 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF051374 Genomic DNA. Translation: AAC05695.1.
3D structure databases
SMR	P34906. Positions 1-327.
ModBase	Search...
Protein family/group databases
REBASE	3402. M.FnuDI.
Enzyme and pathway databases
BRENDA	2.1.1.37. 2040.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTF1_FUSNU

Accession

Primary (citable) accession number: P34906

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 24, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)