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P34906 (MTF1_FUSNU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase FnuDI

Short name=M.FnuDI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase FnuDI
Gene names
Name:fnuDIM
OrganismFusobacterium nucleatum
Taxonomic identifier851 [NCBI]
Taxonomic lineageBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the FnuDI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344Modification methylase FnuDI
PRO_0000087876

Regions

Domain1 – 330330SAM-dependent MTase C5-type

Sites

Active site711 By similarity

Sequences

Sequence LengthMass (Da)Tools
P34906 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 7B0CB6C9721FE91C

FASTA34439,913
        10         20         30         40         50         60 
MKLLSLFSGA GGLDLGFERA GFEIIVANEY DKTIWETYEK NHKAKLIKKD IREILSEELP 

        70         80         90        100        110        120 
KSDGIIGGPP CQSWSEAGSL RGINDPRGKL FYEYIRILKD IQPKFFLAEN VKGMLSKRNT 

       130        140        150        160        170        180 
EAVKDIIKEF EEAGYNVFIK LLNAFDYGVA QDRERVFYVG FRKDLNISNF EFPYPISEKE 

       190        200        210        220        230        240 
RKYLKDSIWD LKDNALPGKD KNKTNADDCI VENHEYLTGS YSTIFMSRNR VRQWEQPAFT 

       250        260        270        280        290        300 
VQASGRQCQL HPQAPTMIKI DKNMYKFVAG KENLYRRLSI RECARIQGFP DTFKFYYTSL 

       310        320        330        340 
EDGYKMVGNA VPVDLAYIIA KRIKETLTDK EKIKKEIRQK TLFD 

« Hide

References

[1]"Method for producing the FnuDI restriction endonuclease and methylase."
Zhang B.-H., Wilson G.G.
Patent number US4988620, 29-JAN-1991
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: D.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF051374 Genomic DNA. Translation: AAC05695.1.

3D structure databases

ProteinModelPortalP34906.
SMRP34906. Positions 1-327.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3402. M.FnuDI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTF1_FUSNU
AccessionPrimary (citable) accession number: P34906
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries