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P34905 (MTB1_BREBE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase BbvI

Short name=M.BbvI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase BbvI
Gene names
Name:bbvIM
OrganismBrevibacillus brevis (Bacillus brevis)
Taxonomic identifier1393 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus

Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GCWGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the BbvI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 374374Modification methylase BbvI
PRO_0000087861

Regions

Domain3 – 347345SAM-dependent MTase C5-type

Sites

Active site921 By similarity

Sequences

Sequence LengthMass (Da)Tools
P34905 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 538127C7B761DC01

FASTA37442,350
        10         20         30         40         50         60 
MKFRKGELFC GPGGLALGAK EAKYMHPETG EVFEFEHAWA NDIDEWACET FRTNICPDRP 

        70         80         90        100        110        120 
DSVVCGDVRE LDIKSLGEKF GEIDAFTFGF PCNDYSIVGE HKGMEGNYGP LYSYGVKILN 

       130        140        150        160        170        180 
EYNPLVFIAE NVGGLQSANE GKAFLGILND LASAGKYGYK LVPHLYKFEE YGVPQRRHRI 

       190        200        210        220        230        240 
IIVGIRKDQD VAFRVPEPTH KEKYRTASEA LADIPEDALN HEFTRHKKKV VEMLNHIAPG 

       250        260        270        280        290        300 
GNAWSESIPE ELRLNVKKVR MSQIYRRLHP DQPSYTVTGS GGGGTHGYHW EEPRALTNRE 

       310        320        330        340        350        360 
RARLQTFPDD YEFIGKKEMV RKQIGMAVPP DGAKIILEAV LKTFARIEYP SINSKWDFES 

       370 
VSAEQVIEEV QEIM 

« Hide

References

[1]Barsomian J.M., Wilson G.G.
Submitted (FEB-1994) to UniProtKB
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

3D structure databases

ProteinModelPortalP34905.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTB1_BREBE
AccessionPrimary (citable) accession number: P34905
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries