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Protein

Modification methylase BbvI

Gene

bbvIM

Organism
Brevibacillus brevis (Bacillus brevis)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GCWGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the BbvI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BbvI (EC:2.1.1.37)
Short name:
M.BbvI
Alternative name(s):
Cytosine-specific methyltransferase BbvI
Gene namesi
Name:bbvIM
OrganismiBrevibacillus brevis (Bacillus brevis)
Taxonomic identifieri1393 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878611 – 374Modification methylase BbvIAdd BLAST374

Structurei

3D structure databases

ProteinModelPortaliP34905.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 347SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST345

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiView protein in InterPro
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF00145. DNA_methylase. 1 hit.
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiView protein in PROSITE
PS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.

Sequencei

Sequence statusi: Complete.

P34905-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFRKGELFC GPGGLALGAK EAKYMHPETG EVFEFEHAWA NDIDEWACET
60 70 80 90 100
FRTNICPDRP DSVVCGDVRE LDIKSLGEKF GEIDAFTFGF PCNDYSIVGE
110 120 130 140 150
HKGMEGNYGP LYSYGVKILN EYNPLVFIAE NVGGLQSANE GKAFLGILND
160 170 180 190 200
LASAGKYGYK LVPHLYKFEE YGVPQRRHRI IIVGIRKDQD VAFRVPEPTH
210 220 230 240 250
KEKYRTASEA LADIPEDALN HEFTRHKKKV VEMLNHIAPG GNAWSESIPE
260 270 280 290 300
ELRLNVKKVR MSQIYRRLHP DQPSYTVTGS GGGGTHGYHW EEPRALTNRE
310 320 330 340 350
RARLQTFPDD YEFIGKKEMV RKQIGMAVPP DGAKIILEAV LKTFARIEYP
360 370
SINSKWDFES VSAEQVIEEV QEIM
Length:374
Mass (Da):42,350
Last modified:February 1, 1994 - v1
Checksum:i538127C7B761DC01
GO

Cross-referencesi

3D structure databases

ProteinModelPortaliP34905.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiView protein in InterPro
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF00145. DNA_methylase. 1 hit.
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiView protein in PROSITE
PS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTB1_BREBE
AccessioniPrimary (citable) accession number: P34905
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 15, 2017
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.