P34903 (GBRA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-aminobutyric acid receptor subunit alpha-3

Alternative name(s):
GABA(A) receptor subunit alpha-3

Gene names

Name:

GABRA3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Subunit structure	Binds UBQLN1 By similarity. Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA3 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Signal Transmembrane Transmembrane helix
Ligand	Chloride
Molecular function	Chloride channel Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	gamma-aminobutyric acid signaling pathway Traceable author statement Ref.1. Source: ProtInc ion transmembrane transport Traceable author statement. Source: Reactome
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GABA-A receptor activity Inferred from electronic annotation. Source: InterPro benzodiazepine receptor activity Traceable author statement Ref.1. Source: ProtInc chloride channel activity Inferred from electronic annotation. Source: UniProtKB-KW extracellular ligand-gated ion channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 492	464	Gamma-aminobutyric acid receptor subunit alpha-3		PRO_0000000437
Regions
	Topological domain	29 – 276	248	Extracellular Probable
	Transmembrane	277 – 298	22	Helical; Probable
	Transmembrane	304 – 325	22	Helical; Probable
	Transmembrane	338 – 359	22	Helical; Probable
	Topological domain	360 – 457	98	Cytoplasmic Probable
	Transmembrane	458 – 479	22	Helical; Probable
Amino acid modifications
	Modified residue	433	1	Phosphoserine By similarity
	Glycosylation	63	1	N-linked (GlcNAc...) Potential
	Glycosylation	163	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Glycosylation	228	1	N-linked (GlcNAc...) Potential
	Disulfide bond	191 ↔ 205		By similarity
Experimental info
	Sequence conflict	180	1	L → P in AAH28629. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P34903 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 23A7390C9822691F	FASTA	492	55,165
	10 20 30 40 50 60 MIITQTSHCY MTSLGILFLI NILPGTTGQG ESRRQEPGDF VKQDIGGLSP KHAPDIPDDS 70 80 90 100 110 120 TDNITIFTRI LDRLLDGYDN RLRPGLGDAV TEVKTDIYVT SFGPVSDTDM EYTIDVFFRQ 130 140 150 160 170 180 TWHDERLKFD GPMKILPLNN LLASKIWTPD TFFHNGKKSV AHNMTTPNKL LRLVDNGTLL 190 200 210 220 230 240 YTMRLTIHAE CPMHLEDFPM DVHACPLKFG SYAYTTAEVV YSWTLGKNKS VEVAQDGSRL 250 260 270 280 290 300 NQYDLLGHVV GTEIIRSSTG EYVVMTTHFH LKRKIGYFVI QTYLPCIMTV ILSQVSFWLN 310 320 330 340 350 360 RESVPARTVF GVTTVLTMTT LSISARNSLP KVAYATAMDW FIAVCYAFVF SALIEFATVN 370 380 390 400 410 420 YFTKRSWAWE GKKVPEALEM KKKTPAAPAK KTSTTFNIVG TTYPINLAKD TEFSTISKGA 430 440 450 460 470 480 APSASSTPTI IASPKATYVQ DSPTETKTYN SVSKVDKISR IIFPVLFAIF NLVYWATYVN 490 RESAIKGMIR KQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of cDNA sequences encoding human alpha 2 and alpha 3 gamma-aminobutyric acidA receptor subunits and characterization of the benzodiazepine pharmacology of recombinant alpha 1-, alpha 2-, alpha 3-, and alpha 5-containing human gamma-aminobutyric acidA receptors." Hadingham K.L., Wingrove P., le Bourdelles B., Palmer K.J., Ragan C.I., Whiting P.J. Mol. Pharmacol. 43:970-975(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Candidate gene analysis in Rett syndrome and the identification of 21 SNPs in Xq." Amir R., Dahle E.J., Toriolo D., Zoghbi H.Y. Am. J. Med. Genet. 90:69-71(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Forbidden fruit - Issue 56 of March 2005

Cross-references

Sequence databases
EMBL GenBank DDBJ	S62908 mRNA. Translation: AAB27279.1. AF166361 , AF165901, AF165902, AF165903, AF165904, AF165905, AF165906, AF166359, AF166360 Genomic DNA. Translation: AAG12455.1. BC028629 mRNA. Translation: AAH28629.1.
IPI	IPI00002897.
PIR	I77911.
RefSeq	NP_000799.1. NM_000808.3.
UniGene	Hs.123024.
3D structure databases
ProteinModelPortal	P34903.
ModBase	Search...
Protein-protein interaction databases
IntAct	P34903. 3 interactions.
MINT	MINT-7242442.
STRING	9606.ENSP00000359334.
PTM databases
PhosphoSite	P34903.
Polymorphism databases
DMDM	462149.
Proteomic databases
PaxDb	P34903.
PRIDE	P34903.
Protocols and materials databases
DNASU	2556.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000370311; ENSP00000359334; ENSG00000011677. ENST00000370314; ENSP00000359337; ENSG00000011677. ENST00000535043; ENSP00000443527; ENSG00000011677.
GeneID	2556.
KEGG	hsa:2556.
UCSC	uc010ntk.1. human.
Organism-specific databases
CTD	2556.
GeneCards	GC0XM151334.
HGNC	HGNC:4077. GABRA3.
HPA	HPA000839.
MIM	305660. gene.
neXtProt	NX_P34903.
Orphanet	79102. Thyrotoxic periodic paralysis.
PharmGKB	PA28491.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG238757.
HOGENOM	HOG000231337.
HOVERGEN	HBG051707.
InParanoid	P34903.
KO	K05175.
OMA	ASPKTTY.
OrthoDB	EOG4X0MSC.
PhylomeDB	P34903.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System. REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	P34903.
Bgee	P34903.
CleanEx	HS_GABRA3.
Genevestigator	P34903.
GermOnline	ENSG00000011677. Homo sapiens.
Family and domain databases
Gene3D	2.70.170.10. 1 hit.
InterPro	IPR006028. GABAA_rcpt. IPR001390. GABAAa_rcpt. IPR005433. GABBAa3_rcpt. IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. [Graphical view]
PANTHER	PTHR18945. PTHR18945. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 2 hits. [Graphical view]
PRINTS	PR01079. GABAARALPHA. PR01616. GABAARALPHA3. PR00253. GABAARECEPTR. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P34903.
ChEMBL	CHEMBL3026.
DrugBank	DB00404. Alprazolam. DB00829. Diazepam. DB00189. Ethchlorvynol. DB01544. Flunitrazepam. DB00690. Flurazepam. DB00186. Lorazepam. DB00371. Meprobamate. DB00683. Midazolam.
GenomeRNAi	2556.
NextBio	10095.
SOURCE	Search...

Entry information

Entry name

GBRA3_HUMAN

Accession

Primary (citable) accession number: P34903
Secondary accession number(s): Q8TAF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 132 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.